Systemic and pulmonary antibody responses of calves to Pasteurella haemolytica after intrapulmonary inoculation.     

J W McBride  R E Corstvet  D B Paulsen  J R McClure  F M Enright 《American journal of veterinary research》1992,53(10):1889-1894

Systemic and pulmonary antibody responses of calves to Pasteurella haemolytica were evaluated by measuring immunoglobulin production in blood for 9 days and in pulmonary lavage fluid for 7 days after intrapulmonary inoculation. Clinical signs, pulmonary lesions, pulmonary and systemic inflammatory response, and amount of antigen in lavage fluid were used to evaluate the response of calves to challenge with P haemolytica. The pulmonary response consisted of production of IgG, IgE, and IgM antibodies to P haemolytica antigens and a 17- to 68-fold increase of cells in lavage fluid 8 hours after inoculation, with a gradual decrease toward normal. Antibodies of the IgM isotype to P haemolytica were demonstrated as early as 8 hours through 7 days after inoculation in 3 of 3 calves. Of the anti-P haemolytica isotypes, IgM was found in the highest concentration. In all of the inoculated calves, IgE was found 1 to 2 days after inoculation, and IgG was found in 2 of 3 inoculated calves from day 1 through 7 after inoculation. Detection of IgG correlated with smaller pulmonary lesions. Immunoglobulin A was not detected in lavage fluid. Serum was evaluated for IgG and IgM antibody response to P haemolytica. Specific IgM was detectable 5 days after inoculation, and IgG was detectable 7 days after inoculation. Pasteurella haemolytica antigens were not detected in serum or plasma. A transient increase in neutrophil count was found 8 hours after inoculation, with return to baseline values by 24 hours after inoculation.(ABSTRACT TRUNCATED AT 250 WORDS)  相似文献   

Prevalence of Cryptosporidium sp in equids in Louisiana   总被引：3，自引：0，他引：3  

S U Coleman  T R Klei  D D French  M R Chapman  R E Corstvet 《American journal of veterinary research》1989,50(4):575-577

In 1985, 22 pony foals reared in a helminth-free environment were tested daily for oocysts of Cryptosporidium sp by use of fecal flotation. Oocysts were found in all foals. Oocysts were first observed in feces collected from foals 9 to 28 days after birth. The mean period of oocyst shedding was 10 days and ranged from 2 to 18 days in individual foals. Diarrhea was observed in 14 of 22 (64%) foals and began before the period of oocyst shedding. Fecal samples also were examined for other infective agents. Salmonella poona was isolated from 1 foal that did not have diarrhea, and coronavirus particles were observed in the feces of 2 foals with diarrhea. Cryptosporidium sp oocysts also were observed in feces of 2 of 17 Thoroughbred foals, 3 of 14 Quarter Horse foals, and 3 of 26 pony foals reared on pastures with their dams. Samples from pasture-reared foals were collected at irregular intervals. Of the 11 Cryptosporidium-positive fecal samples collected from pastured foals, 2 were from foals with diarrhea. A similar survey was conducted during the 1986 foaling season, using the same procedures. Examination of 300 samples from 58 Quarter Horse, Arabian, and pony foals did not detect oocysts. Daily examination of feces from 10 pony foals reared under helminth-free conditions for 30 days also failed to detect Cryptosporidium oocysts.  相似文献   

Detection of Ehrlichia risticii using an avidin-biotin-immunoperoxidase staining system     

M A Breider  G Callahan  R E Corstvet 《Journal of veterinary diagnostic investigation》1989,1(3):215-218

An indirect immunoperoxidase procedure using a specific anti-Ehrlichia risticii monoclonal antibody and an avidin-biotin-peroxidase staining method was used to detect E. risticii antigen in infected P388D1 murine monocytes. Several different methods of cytological fixation were used, including acetone (15 min), 95% ethanol (15 min), Bouin's fixative (5 hr), and 10% buffered neutral formalin (24 hr). The E. risticii organisms were labeled effectively and identified in cells fixed with acetone and ethanol. However, infected P388D1 cells fixed in 10% formalin or Bouin's fixative required enzymatic digestion with 1.0% trypsin for 15 min at 37 C before positive results were evident. This indirect immunoperoxidase avidin-biotin staining procedure proved to be a sensitive assay for the detection of intracellular E. risticii and may be an effective diagnostic procedure for formalin-fixed paraffin-embedded tissue.  相似文献   

Gossypol toxicity in preruminant calves     

RZ ZELSKI  JT ROTHWELL†  RE MOORE‡  DJ KENNEDY§ 《Australian veterinary journal》1995,72(10):394-398

SUMMARY Twenty-four of 57 calves fed a diet containing 33% cotton seed meal (CSM) died between 7 and 15 weeks of age. Initial deaths were not accompanied by premonitory signs, but after CSM withdrawal most calves developed rough coats, anorexia, weakness, ascites and subcutaneous oedema. Those that died had large volumes of serous fluid in the body cavities, hard livers of ‘nutmeg’ appearance, and pulmonary congestion. Histopathologically the livers showed periacinar necrosis in acute cases and periacinar fibrosis in chronic cases. Lungs from several calves had oedema, haemosiderosis and fibrosis in some pulmonary vessels. Atrophy of myocardial fibres was present in most cases. The concentration of free gossypol in the diet was 100 to 220 mg/kg. Ante-mortem and post-mortem findings supported a diagnosis of gossypol poisoning. The deaths continued for 4 weeks after withdrawal of CSM from the diet.  相似文献   

The use of ultrasonography in the reproductive evaluation of boars          下载免费PDF全文

RO Pinho  BS Camilo  DMA Lima  FAC Villadiego  JCM Vergara  HH Shiomi  RE Cardoso  PS Lopes  JD Guimarães 《Reproduction in domestic animals》2018,53(2):393-400

The objective was to study the use of ultrasound as a complementary test in the breeding soundness evaluation in male pigs and study the pattern of echogenicity of the testicular parenchyma in boars of different racial groups. Twenty‐six adult boars from four different racial groups were used, 10 from the Piau breed (group 1), four from the commercial and finishing group (group 2), six Pietrain breed (group 3) and six from the Duroc breed (group 4). All animals were evaluated for breeding soundness evaluation and the ultrasound examination of the testicles. The groups of animals that were evaluated showed no difference in the main semen parameters that were evaluated, except for the sperm volume, concentration of the ejaculated sperm and the supravital staining; the lowest figures were for the animals from the Piau breed (group 1). In relation to the testicular biometrics, Duroc animals (group 4) had a greater scrotal width compared to the other groups. But when we assessed the intensity of pixels of the testicles, there was a difference between groups. The groups 2 (finishing animals), 3 (Pietrain) and 4 had no difference between themselves. Group 3 had greater pixel intensity in relation to group 1. Of the 26 animals studied, five showed an abnormality during ultrasound evaluation, like hydrocele, hyperechoic mass in the testicular parenchyma, cyst in the head of the epididymis and the presence of fluid in the head and tail of the epididymis. The various animal groups studied did not differ in the principal reproductive parameters evaluated, showing that despite the great variability of reproductive traits between breeds and within the same breed, the breeding soundness evaluation, the more complete it is, is essential for the selection of breeders and the ultrasonography of the reproductive system becomes an important addition in this examination.  相似文献   

Analgesic efficacy of an oral transmucosal spray formulation of meloxicam alone or in combination with tramadol in cats with naturally occurring osteoarthritis          下载免费PDF全文

Beatriz P Monteiro  Mary P Klinck  Maxim Moreau  Martin Guillot  Paulo VM Steagall  Daniel K Edge  Jean‐Pierre Pelletier  Johanne Martel‐Pelletier  Dominique Gauvin  Jérôme RE del Castillo  Eric Troncy 《Veterinary anaesthesia and analgesia》2016,43(6):643-651

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A02Effects of housing and short term transportation on hormonal levels and on lymphocyte glucocorticoid and β-adrenergic receptor concentrations in beef calves     

R. ODORE  P. BADINO  C. GIRARDI  R. BARBERO  B. CUNIBERTI  M. TARANTOLA  & G. RE 《Journal of veterinary pharmacology and therapeutics》2006,29(S1):52-53

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G10Effects of herbal extracts on blood prostaglandins, leukotrienes and thromboxanes in the horse: comparison with phenylbutazone     

E. VALLE  R. BARBERO  B. CUNIBERTI  A. BERTUGLIA  G. RE  & D. BERGERO 《Journal of veterinary pharmacology and therapeutics》2006,29(S1):209-210

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Repeated grading of weed abundance and multivariate methods to improve the efficacy of on-farm weed control trials     

LIBÈRE NKURUNZIZA  PER MILBERG 《Weed Biology and Management》2007,7(2):132-139

We evaluated whether new information could be drawn from additional data collection and unconventional statistical analyses of an on-farm trial. First, we compared a conventional sampling method using a biomass estimate of weed abundance to repeated visual assessment of the percentage ground cover of weeds. The biomass was sampled once after the treatment, whereas the ground cover was repeatedly sampled once before weed control plus several occasions after weed control. Second, we contrasted the outcomes from analysis of variance ( anova ), taking samples from a single point in time with repeated measures (rm) anova and a multivariate method. As the outcomes and conclusions drawn were relatively similar, we conclude that the ground cover estimate of weed abundance was as reliable as the biomass estimate. The rm anova enabled us to follow the temporal trend in response to treatments in the most abundant species, including possible initial differences. Multivariate analysis went even further, by clearly displaying species-wise responses and treatment selectivity.  相似文献   

Expression Pattern of Apoptotic Genes in Vitrified‐Thawed Bovine Oocytes     

VM Anchamparuthy  RE Pearson  FC Gwazdauskas 《Reproduction in domestic animals》2010,45(5):e83-e90

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