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Hayashi M Hirai R Ishihara Y Horiguchi N Endoh D Okui T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2007,69(10):1039-1045
Combined effects of treatment with trientine, a copper-chelating agent, and X-irradiation on development of fibrosarcoma using a murine transplantation model in vivo and on cellular survival in vitro were examined. Copper contents in the tumors and serum of trientine-treated mice were significantly lower than those of untreated mice. The tumor volumes of mouse fibrosarcoma QRsp-11 cells increased more slowly in the trientine-treated and the X-irradiated mice than in the control mice from 10 to 24 days postinoculation. The extent of inhibition of tumor growth by X-irradiation at 3 Gy was similar to that obtained by treatment with trientine. A combination of trientine and X-irradiation at 3 Gy showed inhibitory effects on tumor growth similar to those obtained by X-irradiation at 6 Gy. The results showed that trientine and X-irradiation interacted additively in inhibition of tumor growth. When QRsp-11 cells and mouse and bovine endothelial cells were treated with trientine after X-irradiation, the surviving fractions of the cells with combined treatments were essentially consistent with the products of the surviving fractions of trientine-treated cells and those of X-irradiated cells. When the cells were pretreated with trientine and X-irradiated, the surviving fractions of the pretreated cells were lower than those of cells without treatment. 相似文献
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通过1980～1987年秋季在大连老铁山、山东省长岛县大黑山岛及青岛浮山的观察研究,确认猛禽的迁徙与气候、天气有着密切的关系。猛禽迁徙通常选择在风力三级(14 km/h)以上西北风或南风的有利天气条件下顺风或侧顺风迁徒。这种大气环流形势在天气图上的反映是地面冷锋过境后当天或次日。冷锋过境前的天气特征是偏南风或西南风,气温升高,冷锋过境后风向转为西或西北风,同时气温下降,气压升高,风速加大。5～6级西北风(30～40 km/h)天气会出现猛禽大规模迁徙过境。故在猛禽的研究中,可根据这种天气变化模式进行猛禽迁徙的预报。 相似文献
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Tsutomu Hattori Yoji Narimatsu Masaki Ito Yuji Ueda Kunihiro Fujiwara Daiji Kitagawa 《Fisheries Science》2007,73(2):341-347
ABSTRACT: The biomass of bighand thornyhead Sebastolobus macrochir was increased by the high recruitment success of the 1999–2002 year classes off the Pacific coast of northern Honshu, Japan. In this study, the growth of bighand thornyhead was examined over a 9-year period from 1996 to 2004 in this area. The growth of the 1999 year class and the 2000–2002 year classes was reduced at 3 and 2 years old, respectively, while the 1999–2002 year classes were smaller than the 1993–1998 year classes. In 2-, 3- and 4-year-old fish, the relationship between abundance and mean standard length was expressed by negative linear regressions, while fish became smaller when abundance of the year class was larger. Mean bottom temperatures were stable at depths of 350–900 m; variations in water temperature were small in the main distribution area of bighand thornyhead. We discuss the factors affecting the growth of bighand thornyhead via changes in the demersal fish community and feeding habits. 相似文献
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Namiki Y Kon Y Sasaki N Agui T Endoh D 《The Japanese journal of veterinary research》2004,52(3):125-134
In MRL/MpJ mice, there is a genetic mutation of exonuclease 1 (Exo1), in which the exon 9 is sometimes deleted. In the present study, to check the generation of the spliced exons, exon 8-intron 8-exon 9 (pCX/Ex/EIE/B and pCX/Ex/EIE/M) plasmids were temporally transfected in vitro into BALB 3T3 cells, and RT-PCR using appropriate primer pair was carried out 1 day after transfection. In these constructions, pCX/Ex/EIE/B was derived from genomic sequence of C57BL/6 mice, and pCX/Ex/EIE/M was from MRL/MpJ. A spliced band was detected in pCX/Ex/EIE/B, but was present little or very weakly in pCX/Ex/EIE/M. Next, the same spliced band was demonstrated in the pCX/Ex/EIE/M(T) plasmid, in which the branchpoint sequence (BPS) of pCX/Ex/EIE/M including the exon 9 was changed into that of pCX/Ex/EIE/B. The splicing did not occur in the dell1/B mutant, in which 1960 nucleotides of the intron 8 were deleted, whereas it was detected in the del2/B plasmid deleted 1036 nucleotides in its middle region. These results suggest that the nucleotide T to A mutation of the BPS in the intron 8 is at least a sufficient for generation of splice variants (tr-1 and tr-2 Exo1). 相似文献
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Yasuda D Fujita M Yasuda S Taniguchi A Miura H Hasegawa D Orima H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2004,66(11):1447-1451
We evaluated the usefulness of MRI and compared it with CT for diagnosis of mesenteric lymphoma in a dog. The results in the plain CT, dynamic CT and plain MR (T1WI and T2WI) images suggested that the mass was a large single nodular lesion with abundant blood perfusion. On enhanced MRI(T1WI) , the mass was depicted as a tumor with adhesion to the gut wall. Exploratory laparotomy confirmed the mass was consistent with the findings on enhanced MRI. We think that MRI might be a useful imaging tool for diagnosis of canine mesenteric lymphoma. 相似文献
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Fujita M Takaishi Y Yasuda D Hasegawa D Taniguchi A Takahashi K Orima H 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2008,70(5):525-528
Magnetic resonance (MR) was conducted for an 8-year-old, intact male Spitz with sneezing, serous discharge and epistaxis from the left nasal cavity. MR imaging showed a nasal cavity-occupied mass of iso-intensity on T1WI , high-intensity on T2WI and markedly enhanced on contrast-enhanced T1WI at parts of rostal to medial ocular angle in the left cavity. After Surgery and intraoperative radiation, the mass was diagnosed intranasal hemangiosarcoma by histopathology. Although the dog showed the finding, which suggested recurrence after the treatment ending, about 30 months later, it maintained good conditions without evidence of metastasis. 相似文献
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Osamu Yamato Asogi Kobayashi Hiroyuki Satoh Daiji Endoh Toru Shoda Yukiko Masuoka Ayano Hatakeyama Eun-Og Jo Tomoya Asano Madoka Yonemura Masahiro Yamasaki Yoshimitsu Maede 《Journal of veterinary diagnostic investigation》2004,16(4):299-304
In the present study, diagnostic methods for canine G(M1)-gangliosidosis were examined by comparing a DNA mutation assay with an enzyme assay. Sixty-two Shiba dogs of a pedigree with G(M1)-gangliosidosis were differentiated into 3 genotypes, i.e., normal, heterozygous, and homozygous affected dogs, using a DNA mutation assay, which consists of polymerase chain reaction amplification and the determination of restriction fragment length polymorphisms. The beta-galactosidase activity in leukocytes, umbilical cords, and plasma was measured using 4-methylumbelliferyl beta-D-galactoside and p-nitrophenyl beta-D-galactoside as artificial substrates and compared among the 3 genotypes. The results showed that it was possible to identify homozygous dogs with the enzyme assay using leukocytes and umbilical cords. When using leukocytes, heterozygous carriers could be differentiated from normal dogs in many cases. However, the use of the DNA mutation assay is essential for a complete determination of heterozygous carriers because of the overlap in the distribution of enzyme activity between these 2 groups. When umbilical cords were used, heterozygous carriers could not be differentiated from normal dogs because of no significant difference in enzyme activity between these 2 groups. The beta-galactosidase activity in plasma was not applicable to the diagnosis and genotyping of G(M1)-gangliosidosis in Shiba dogs. 相似文献
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Yutaka Kanae Daiji Endoh Hajime Nagahata Masanobu Hayashi 《Journal of veterinary diagnostic investigation》2005,17(3):258-262
Complex vertebral malformation (CVM), a hereditary lethal disease in Holstein calves, is characterized by complex anomalies of the vertebral column and limbs in an aborted fetus and in prematurely born, stillborn, and neonatal calves. The mode of inheritance of CVM is autosomal recessive, and CVM is caused by a point mutation from G to T at nucleotide position 559 of the bovine solute carrier family 35 member 3 (SLC35A3) gene. Although an allele-specific polymerase chain reaction (AS-PCR) is a useful method for diagnosis of CVM, the AS-PCR requires selected DNA polymerases and strictly controlled reaction conditions to obtain reliable results. Therefore, an alternative screening method for the CVM gene would be useful. Polymerase chain reaction-primer introduced restriction analysis (PCR-PIRA) is a method that can be used for detecting a single nucleotide mutation in any gene without a restriction site around the mutation site. In this study, primers were designed to introduce PstI or EcoT22 sites into PCR products from the wild-type and CVM alleles, respectively. The wild-type allele, a heterozygote, and a homozygote of the CVM allele could be discriminated by restriction fragment length polymorphism analysis. Specific introduction of restriction sites into PCR products depending on the change in a single nucleotide of template was shown using a variety of DNA polymerases and PCR machines. Therefore, the PCR-PIRA technique using primers designed in this study might provide a more useful method for extensive screening of CVM. 相似文献
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玉米种植体系土壤磷素有效性对有机肥长期施用响应的Meta分析 总被引：2，自引：0，他引：2
长期施用有机肥能够提高土壤有效磷含量,但不同的研究间差异较大,通过基于有机肥种类、施用量、有机肥替代化肥比例和土壤因素等大量试验数据的整合分析,研究不同因素下土壤磷素有效性对有机肥长期施用的响应,对指导有机肥的科学施用具有重要意义。本研究收集了公开发表的文献147篇,建立了305组包含单施化肥（CF）和化肥配施有机肥（CFM）处理的土壤有效磷含量数据库。采用Meta分析研究全国多个长期定位试验点的CF和CFM处理下土壤有效磷含量差异及其影响因素。结果表明,CFM处理能显著提高土壤有效磷含量,每投入100 kg·hm-2的磷,土壤有效磷增加量约为CF处理的2.6倍。高施磷量（>300kg·hm-2）对土壤有效磷含量的提升幅度,约是低施磷量（<75 kg·hm-2）和中等施磷量（75~150 kg·hm-2和150~300 kg·hm-2）的3.5倍和2.3~2.6倍。不同有机肥对土壤有效磷含量的影响表现为畜禽粪便类>商品有机肥类>秸秆类>城市垃圾类有机肥。其中,畜禽粪便类中,猪粪、鸡粪和牛粪对土壤有效磷含量的影响较为显著,增幅分别为210.4%、209.3%和156.7%。在不同有机肥替代化肥比例下,施用有机肥对土壤有效磷含量的提升效果,随有机肥替代化肥比例的增加而升高,且差异显著,有机肥替代化肥比例为75%和100%时,增幅可达211.7%和239.7%。土壤类型、有机质含量、pH等土壤因素是影响施用有机肥对土壤有效磷含量提升效果的重要因素。其中,不同土壤类型下土壤有效磷增幅在12.7%~212.1%之间,表现为红壤>潮土>褐土>黑土>棕壤;在土壤有机质含量为0.6%~1%时,土壤有效磷增幅可达248.5%,分别是土壤有机质含量<0.6%、1%~2%和2%~3%时的2.1、2.0倍和10.9倍;当pH为酸性（5~6.5）或者pH为中性（6.5~7.5）时,施用有机肥对土壤有效磷的提升效果显著高于pH为碱性（7.5~9）时。此外,土壤有效磷增幅随年均降雨量和年均温度的升高而增加,其中年均降雨量>800 mm或年均温度>16℃时增幅最大。长期施用不同种类有机肥、施用量、有机肥替代化肥比例均显著影响土壤有效磷含量,并且在不同的土壤环境、轮作方式和气候因子条件下,施用有机肥对土壤有效磷的效应有显著差异。因此,在农业生产过程中,科学施用有机肥不仅要充分考虑有机肥种类、施用量、有机肥替代化肥比例,而且要综合考虑不同环境因素的影响,从而保障有机废弃物资源的科学利用,避免资源浪费和环境污染,推动农业绿色高质量和可持续发展。 相似文献