Correction to: Predation on glass eels of Japanese eel <Emphasis Type="Italic">Anguilla japonica</Emphasis> in the Tone River Estuary,Japan     

Yoichi Miyake  Aigo Takeshige  Hikaru Itakura  Hajime Itoh  Hiroaki Onda  Akira Yamaguchi  Akihito Yoneta  Kohma Arai  Yulina V. Hane  Shingo Kimura 《Fisheries Science》2018,84(6):1015-1016

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Accuracy of imputation of single nucleotide polymorphism marker genotypes from low‐density panels in Japanese Black cattle          下载免费PDF全文

Shinichiro Ogawa  Hirokazu Matsuda  Yukio Taniguchi  Toshio Watanabe  Akiko Takasuga  Yoshikazu Sugimoto  Hiroaki Iwaisaki 《Animal Science Journal》2016,87(1):3-12

Using target and reference fattened steer populations, the performance of genotype imputation using lower‐density marker panels in Japanese Black cattle was evaluated. Population imputation was performed using BEAGLE software. Genotype information for approximately 40 000 single nucleotide polymorphism (SNP) markers by Illumina BovineSNP50 BeadChip was available, and imputation accuracy was assessed based on the average concordance rates of the genotypes, varying equally spaced SNP densities, and the number of individuals in the reference population. Two additional statistics were also calculated as indicators of imputation performance. The concordance rates tended to be lower for SNPs with greater minor allele frequencies, or those located near the ends of the chromosomes. Longer autosomes yielded greater imputation accuracies than shorter ones. When SNPs were selected based on linkage disequilibrium information, relative imputation accuracy was slightly improved. When 3000 and 10 000 equally spaced SNPs were used, the imputation accuracies were greater than 90% and approximately 97%, respectively. These results indicate that combining genotyping using a lower‐density SNP chip with genotype imputation based on a population of individuals genotyped using a higher‐density SNP chip is a cost‐effective and valid approach for genomic prediction.  相似文献   

Androgen induces production of male effect pheromone in female goats   总被引：1，自引：0，他引：1  

Kakuma Y  Ichimaru T  Yonezawa T  Momozawa Y  Hashizume C  Iwata E  Kikusui T  Takeuchi Y  Ohkura S  Okamura H  Mori Y 《The Journal of reproduction and development》2007,53(4):829-834

Previously we showed that the primer pheromone responsible for the "male effect" was produced in specific skin regions of castrated male goats by androgen treatments. In the present study, we examined whether androgen can also induce production of the male effect pheromone in female goats. Capsules containing dihydrotestosterone (DHT) or testosterone (T) were subcutaneously implanted into six ovariectomized (OVX) goats for 28 days. Small skin samples were collected from the head and rump regions, and the pheromone activity of their ether extracts was examined using a bioassay that monitors the electrophysiological manifestation of the hypothalamic gonadotropin-releasing hormone pulse generator as multiple-unit activity. Behaviors of OVX goats towards ovary-intact estrous goats were also examined before and at the end of DHT or T treatment. Before androgen treatment, neither the head nor rump skin samples in OVX goats showed pheromone activity. DHT treatment induced pheromone activity in the head skin sample of six OVX goats and in the rump skin sample of two OVX goats. Similar results were obtained by T treatment. In addition, OVX goats treated with T showed masculine-type sexual behaviors such as courtship and mounting behaviors towards the estrous goats. These results demonstrate that androgen is capable of inducing primer pheromone activity in the female and suggest that the synthesis pathway of the male effect pheromone exists in both sexes in the goat.  相似文献   

Effects of a macrolide antibiotic on enamel formation in rat incisors--primary lesion of ameloblast at the transition stage     

Abe T  Miyajima H  Okada K 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(9):985-988

A novel macrolide antibiotic was administered orally to 5-week-old Jcl:Wistar rats at a dose of 5,000 mg/kg/day for 5 weeks, and then a half of animals were maintained without any treatment for 10 weeks. A white discolored lesion with horizontal stripes developed on the surface of the upper and lower incisors after dosing for 4 weeks, and these macroscopical incisal lesions disappeared with the eruption in 4 weeks after stop of administration. Histopathologically, increase in number of karyopycnosis of ameloblast at the transitional stage, vacuolar degeneration of ameloblast and cystic change in the maturation stage, and impaired iron pigment secretion at the pigmentation stage were observed. Microradiography, calcio-traumatic zones, which means hypocalcification, were observed on the superficial layer of enamel. These results suggest that the primary lesion induced by a novel macrolide antibiotic is the increased karyopycnosis of ameloblast at the transitional stage, and followed by later stage.  相似文献   

Down regulation of a novel protein,PSP, in rat hepatoma cdRLh 84-beared tumor     

Kaneki K  Kanouchi H  Matsumoto M  Kawasaki Y  Akuzawa M  Oka T 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2003,65(7):781-785

Perchloric acid-soluble protein (PSP) is a members of a new hypothetical family (YER057c/YJGF family) of small proteins with presently unknown function. The high degree of evolutionary conservation of these proteins reflect an involvement in basic cellular regulation. The expression of PSP was examined in rat hepatoma cell dRLh 84-beared rats. The tumor weight increased to 4.24 g at 3 weeks after the transplantation of hepatoma cells and hepatoma which has less differentiated characteristics were observed in rat liver. The expression of PSP in rat hepatoma was down regulated as compared with intact tissue. Thus the expression of PSP seems to be associated with the differentiation process in these transformed cells. On the other hand, some positive cells against the PSP-antibody were observed in the central region of tumor tissue by immunohistochemistry. These cells were shown to be the lymphocytes and the macrophages. The involvement of PSP to cellular growth and differentiation is discussed.  相似文献   

Genetic and biological comparison of tick-borne encephalitis viruses from Hokkaido and far-eastern Russia     

Goto A  Hayasaka D  Yoshii K  Mizutani T  Kariwa H  Takashima I 《The Japanese journal of veterinary research》2002,49(4):297-307

We compared the biological properties of Oshima 5-10 (tick-borne encephalitis [TBE] virus isolated in Hokkaido, Japan) and Sofjin-HO (Far-Eastern subtype TBE virus) including plaque formation, virus replication and virus protein synthesis in BHK-21 cell cultures to reveal strain differences. We also determined the complete nucleotide sequences of both strains and compared the deduced amino acid sequences. Plaques of Oshima 5-10 were smaller than those of Sofjin-HO. Virus titers in culture fluid of Oshima 5-10 were 1/100 of those of Sofjin-HO at 9 and 12 hr after infection. Less viral protein and RNA syntheses of strain Oshima 5-10 was observed than with Sofjin-HO. Genetic analysis revealed 1.4% of amino acids to differ with Sofjin-HO. No difference between the two strains was detected in the motif sequence of the viral enzyme, cleavage sites of viral protein or glycosylation sites of NS1.  相似文献   

中国锦紫苏类病毒的检测及其分子生物学特征研究   总被引：4，自引：0，他引：4  

苏前富  郭瑞  成卓敏  SANO TERUO  李世访 《植物病理学报》2006,36(3):226-231

 从无明显症状表现的11株锦紫苏叶片中抽提低分子量的RNA,经Return-PAGE、RT-PCR和Dot-blot hybridization检测,结果表明11株锦紫苏全部带有锦紫苏类病毒(Coleus blumei viroid,CBVd)。将部分PCR产物克隆到pGEM-3Zf(+)载体上并进行DNA序列测定。序列分析结果,所克隆的序列(GenBank登录号分别为DQ178395、DQ178396、DQ178397、DQ178398和DQ178399)与GenBank中报道的锦紫苏类病毒1号(CBVd-1)序列同源性为85.23%~99.20%。从市场上购买的锦紫苏种子经Return-PAGE和RT-PCR检测不携带锦紫苏类病毒。这是中国发生的锦紫苏类病毒的首次报道。  相似文献   

Productivity of kayu putih (Melaleuca leucadendron LINN) tree plantation managed in non-timber forest production systems in Java,Indonesia     

BudiadiEmail author  Yoichi?Kanazawa  Hiroaki?T.?Ishii  M.?Sambas?Sabarnurdin  Priyono?Suryanto 《Agroforestry Systems》2005,64(2):143-155

Kayu putih oil, extracted from the kayu putih tree (Melaleuca leucadendron), is an important non-timber forest product (NTFP) in Indonesia. We compared productivity of kayu putih tree plantations among three different sites in East, Central, and West Java, Indonesia. Leaf-twig, and branch production per tree differed significantly among the three sites. Tree biomass production was highest at the site in West Java practicing tumpangsari (a form of shifting cultivation) of kayu putih with rice (Oryza sativa) on alluvial soils. Tree biomass production was lowest at the site in East Java, practicing tumpangsari of kayu putih with maize (Zea mays) and cassava (Mani- hot esculenta). At this site, tree biomass production increased with increasing stand age and low production was compensated by greater biomass yield of companion crops. Intermediate tree biomass production was observed at the site in Central Java where intercropping is only practiced for the first two years after plantation establishment. Production of tree biomass and kayu putih oil did not increase with increasing stand density, indicating that at a given site, high tree stocking did not lead to high production. Stand-level productivity of the plantations increased with increasing stand age and maximum productivity was attained at 15 to 35 years, after which productivity declined. This suggested unsustainable stand productivity as plantations increase in age.  相似文献   

The pharmacological effects of the anesthetic alfaxalone after intramuscular administration to dogs     

Jun TAMURA  Tomohito ISHIZUKA  Sho FUKUI  Norihiko OYAMA  Kodai KAWASE  Kenjiro MIYOSHI  Tadashi SANO  Kirby PASLOSKE  Kazuto YAMASHITA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(3):289-296

The pharmacological effects of the anesthetic alfaxalone were evaluated after intramuscular (IM) administration to 6 healthy beagle dogs. The dogs received three IM doses each of alfaxalone at increasing dose rates of 5 mg/kg (IM5), 7.5 mg/kg (IM7.5) and 10 mg/kg (IM10) every other day. Anesthetic effect was subjectively evaluated by using an ordinal scoring system to determine the degree of neuro-depression and the quality of anesthetic induction and recovery from anesthesia. Cardiorespiratory variables were measured using noninvasive methods. Alfaxalone administered IM produced dose-dependent neuro-depression and lateral recumbency (i.e., 36 ± 28 min, 87 ± 26 min and 115 ± 29 min after the IM5, IM7.5 and IM10 treatments, respectively). The endotracheal tube was tolerated in all dogs for 46 ± 20 and 58 ± 21 min after the IM7.5 and IM10 treatments, respectively. It was not possible to place endotracheal tubes in 5 of the 6 dogs after the IM5 treatment. Most cardiorespiratory variables remained within clinically acceptable ranges, but hypoxemia was observed by pulse oximetry for 5 to 10 min in 2 dogs receiving the IM10 treatment. Dose-dependent decreases in rectal temperature, respiratory rate and arterial blood pressure also occurred. The quality of recovery was considered satisfactory in all dogs receiving each treatment; all the dog exhibited transient muscular tremors and staggering gait. In conclusion, IM alfaxalone produced a dose-dependent anesthetic effect with relatively mild cardiorespiratory depression in dogs. However, hypoxemia may occur at higher IM doses of alfaxalone.  相似文献   

Combination of serum phosphorylated neurofilament heavy subunit and hyperintensity of intramedullary T2W on magnetic resonance imaging provides better prognostic value of canine thoracolumbar intervertebral disc herniation     

Tadahisa MASHITA  Hiroaki KAMISHINA  Yuya NAKAMOTO  Yosuke AKAGI  Ataru NAKANISHI  Yusuke HARASAKI  Tsuyoshi OZAWA  Takashi UEMURA  Yui KOBATAKE  Shunsuke SHIMAMURA  Naoki KITAMURA  Sadatoshi MAEDA  Yuji UZUKA  Gerry SHAW  Jun YASUDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2015,77(4):433-438

The aim of this study was to evaluate the prognostic value of concurrent measurement of serum phosphorylated neurofilament heavy subunit (pNF-H) concentration and intramedullary T2W hyperintensity in paraplegic to paraplegic dogs. Our hypothesis was that concurrent measurement of these would provide a more accurate prediction of functional outcome in dogs with thoracolumbar intervertebral disc herniation (IVDH). A prospective case-control clinical study was designed using 94 dogs with acute onset of thoracolumbar IVDH. The association of serum pNF-H concentration, T2W hyperintensity on sagittal MRI (T2H/L2), deep pain perception and surgical outcome were evaluated with logistic regression analysis after three months for all 94 surgically treated dogs. Sensitivity to predict non-ambulatory outcome was compared among pNF-H and T2H/L2 and combination of both. Logistic regression analysis indicated that serum pNF-H concentration and T2H/L2 were significantly correlated with surgical outcome (P<0.05); however, deep pain perception was not (P=0.41). The results of logistic regression analysis indicated that the odds ratios of unsuccessful long-term outcome were 2.6 for serum pNF-H concentration, 1.9 for T2H/L2 and 2.3 for deep pain sensation. The sensitivity and specificity to predict non-ambulatory outcome for using serum parameter pNF-H>2.6 ng/ml, using T2H/L2 value of>0.84 and using both serum pNF-H and T2H/L2, were 95% and 75.7%, 65% and 86.5%, and 90.0% and 97.5%, respectively. Therefore, combined measurements of serum pNF-H and T2H/L2 might be useful for predicting long-term outcome in dogs with thoracolumbar IVDH.  相似文献