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Soil food webs cycle nutrients and regulate parasites and pathogens, services essential for both agricultural productivity and ecosystem health. Nematodes provide useful indicators of soil food web dynamics. This study was conducted to determine if nematode soil food web indicators and crop yield can be enhanced by combinations of cover crops in a conservation tillage system. The effects of three cover crop treatments (vetch/pea, oat/wheat and oat/wheat/pea/vetch) with low, medium and high C:N and a bare fallow control were investigated in Davis, CA. Nematode fauna, soil properties and plant productivity were measured. Soil food web indices, including the Enrichment Index (EI), Structure Index (SI), Basal Index (BI), and Channel Index (CI), based on the composition of nematode assemblages, were calculated to infer soil food web condition. Cover cropped tomato/corn rotations had twice the number of enrichment opportunist bacterial feeding nematodes, active participants in nitrogen mineralization, than fallowed tomato/corn rotations (opportunist bacterial feeders = 163 versus 98). In winter fallowed plots food webs were basal, common in disturbed, nutrient-poor conditions (BI = 37). Total number of enrichment opportunist nematodes, soil NH4-N levels, and inferred nitrogen mineralization, were higher in cover crop treatments with low to mid C:N ratios. Omnivore and predator nematodes were scarce, averaging less than 6 nematodes 100 g?1 in all treatments. In year one, plant productivity was highest after fallow. In contrast, in year two productivity was highest after cover crops with high nitrogen content and productivity significantly correlated with the structure of the soil fauna. Monitoring the abundance of enrichment opportunists may provide managers with a new tool to evaluate soil food web nitrogen mineralization and plant productivity.  相似文献   
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Live weight gains of light and heavy calves grazing Plains Old World bluestem at three stocking rates were evaluated during the summers of 1997 and 1998. Initial weights of mixed-breed light-weight steers (LHT) were 141 SD = 17 kg (n = 214) in 1997 and 160 SD = 23 kg (n = 193) in 1998. Initial weights of mixed-breed heavy steers (HWT) were 265 SD = 17 kg (n = 115) in 1997 and 248 SD = 13 kg (n = 126) in 1998. Initial stocking rates for both sizes of steers were as follows: light, 392 kg of live weight/ha; moderate, 504 kg of live weight/ha (increased to 616 kg live weight/ha in 1998); and heavy, 840 kg of live weight/ ha. Averaged gain and gain/hectare are reported as stocking rate by steer type within year. Heavy steers had greater ADG than LHT steers during both years. Forage intake, expressed as a percentage of BW, was greater (P = 0.05) for LHT (3.1%) than for HWT (2.8%) calves. Grazing time (min/d; 1998 only) was greater (P = 0.05) for LHT (665) than for HWT (624) steers. Forage CP and in vivo digestible organic matter (DOM) were slightly greater (P < 0.05) in pastures grazed by HWT vs LHT cattle. Gain/hectare was greater (P < 0.05) for LHT than for HWT calves at all three stocking rates during both years. A linear decline in ADG was observed (P < 0.07) as stocking rates increased for HWT steers in 1997 and LHT steers in 1998. However, ADG did not decline with increasing stocking rate for LHT calves during 1997 or HWT calves during 1998. Forage intake was not different among stocking rates in either 1997 or 1998. Grazing time was greatest (P < 0.05) for steers in the moderate and heavy stocking rates. Forage in vivo DOM decreased (P < 0.05) as stocking rate increased. Both LHT and HWT steers had lower (P < 0.05) ADG at all three stocking rates during 1998 compared with 1997. Despite lower ADG, LHT steers had greater gain/hectare than HWT steers during both 1997 and 1998.  相似文献   
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Fall-weaned crossbred steer calves (n = 300; 184 +/- 2.9 kg) received either no implant (Control) or were implanted with Synovex-C (SC = 10 mg estradiol benzoate + 100 mg progesterone), Synovex-S (SS = 20 mg estradiol benzoate + 200 mg progesterone), or Revalor-G (RG = 8 mg estradiol-17beta + 40 mg trenbolone acetate) to determine the effects of implants on weight gain during winter grazing on dormant tallgrass prairie, subsequent grazing and finishing performance, and carcass characteristics. Steers grazed two dormant tallgrass prairie pastures from October 16, 1996, until March 29, 1997 (164 d), and received 1.36 kg/d of a 25% CP supplement that supplied 100 mg of monensin/steer. Following winter grazing, all steers were implanted with Ralgro (36 mg zeranol) and grazed a common tallgrass prairie pasture until July 17 (110 d). After summer grazing, all steers were implanted with Revalor-S (24 mg estradiol-17beta + 120 mg trenbolone acetate), and winter implant treatment groups were equally allotted to four feedlot pens. Steers were harvested November 17, 1997, after a 123-d finishing period. Daily gains during the winter grazing phase averaged .28, .32, .32, or .35 kg/d, respectively, for Control, SC, SS, or RG steers and were greater (P < .01) for implanted steers than for Controls. Summer daily gains were similar (1.05 +/- .016 kg/d; P > or = .61) for all treatment groups. Feedlot daily gains were also similar (1.67 +/- .034 kg/d; P > or = .21), with implanted steers weighing 14 kg more than Control steers (P = .05) at harvest, despite similar management during summer grazing and feedlot phases. Control steers tended (P = .06) to have lower yield grades. There were no differences (P = .99) in marbling between implanted and nonimplanted steers. Steers implanted during the wintering phase had increased skeletal and overall (P < .01) carcass maturities compared with nonimplanted steers, which resulted in more "B" and "C" maturity carcasses. Because carcass maturity score affects quality grade, the increased maturities of implanted steers resulted in a $9.04 decrease in carcass value/100 kg (P < .01) compared with Controls. The results of this study indicate that growth-promoting implants are efficacious for cattle wintered on dormant native range despite low daily gains. This increased weight is maintained through the summer grazing and feedlot phases; however, the benefit of the increased weight may be offset by decreased carcass quality grade and value due to increased carcass maturity.  相似文献   
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Experiments were conducted to determine the effects of increasing supplement protein concentration on performance and forage intake of beef cows and forage utilization of steers consuming stockpiled bermudagrass forage. Bermudagrass pastures were fertilized with 56 kg of N/ha in late August. Grazing was initiated during early November and continued through the end of January each year. Treatments for the cow performance trials were: no supplement or daily equivalents of 0.2, 0.4, and 0.6 g of supplemental protein per kilogram of BW. Supplements were formulated to be isocaloric, fed at the equivalent of 0.91 kg/d, and prorated for 4 d/wk feeding. Varying the concentration of soybean hulls and soybean meal in the supplements created incremental increases in protein. During yr 1, supplemented cows lost less weight and condition compared to unsupplemented animals (P < 0.05). During yr 2, supplemented cows gained more weight (P = 0.06) and lost less condition (P < 0.05) compared to unsupplemented cows. Increasing supplement protein concentration had no affect on cumulative cow weight change or cumulative body condition score change. Forage intake tended to increase (P = 0.13, yr 1 and P = 0.07, yr 2) in supplemented cows. Supplement protein concentration did not alter forage intake. In a digestion trial, four crossbred steers were used in a Latin square design to determine the effects of supplement protein concentration on intake and digestibility of hay harvested from stockpiled bermudagrass pasture. Treatments were no supplement; or 0.23, 0.46, and 0.69 g of supplemental protein per kilogram of BW. Forage intake increased (P < 0.05) 16% and OM intake increased (P < 0.01) 30% in supplemented compared to unsupplemented steers. Diet OM digestibility increased (P = 0.08) 14.5% and total digestible OM intake increased (P < 0.05) 49% in supplemented compared to unsupplemented steers. Supplement protein concentration did not alter forage intake, total digestible OM intake, or apparent digestibility of OM or NDF. During the initial 30 d after first killing frost, beef cows did not respond to supplementation. However, later in the winter, supplementation improved utilization of stockpiled bermudagrass forage.  相似文献   
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Beta-adrenoceptors are important regulators of cardiac function and their characteristics are known to change in human and canine diseased myocardium. This study aimed to determine the density and subtypes of beta-adrenoceptors in the normal and failing equine ventricular myocardium. Membrane preparations of the left papillary muscles were incubated with increasing concentrations of the nonselective beta-adrenoceptor antagonist [3H]-CGP12177. Saturable and reversible binding of [3H]-CGP12177 to myocardial membranes was demonstrated with Kd values (+/- s.d.) of 0.49 +/- 0.40 and 0.43 +/- 0.22 nmol/l and Bmax values of 93.4 +/- 20.5 and 110.0 +/- 21.2 and fmol/mg protein for normal (n = 19) and heart failure (n = 10) tissues, respectively. Heart failure had no significant effect on the density of ventricular beta-adrenoceptors. The cardiac beta-adrenoceptors were further characterised by studying displacement of [3H]-CGP12177 (0.6 nmol/l) with the beta1-selective antagonists CGP20712A and the beta2-selective antagonist ICI118.551. In normal ventricular muscle, CGP20712A was 26 times more potent than ICI118.551 (Ki values 30.4 +/- 24.8 and 814.1 +/- 485.2 nmol/l, respectively). In heart failure cases, CGP 20712A curves were monophasic with a Ki value of 45.6 +/- 39.7 nmol/l. ICI 118.551 curves were biphasic in 5 horses where 11-31% of the cardiac beta-adrenoceptors had a high affinity for ICI 118.551. These data suggest that the normal equine ventricular myocardium possesses predominately beta1-adrenoceptors, with no evidence for co-existence of a significant population of beta2-adrenoceptors. The density of beta-adrenoceptors did not appear to change in heart failure, but the appearance of receptors with a high affinity for ICI118.551 may suggest that, in some cases, heart failure increases the expression of beta2-adrenoceptors in equine ventricular myocardium. This study provides an insight into the role of the adrenergic system in heart disease in the horse. Further studies in this area are warranted.  相似文献   
8.
A 4-year-old, entire female, English Cocker Spaniel was presented for treatment of lymphosarcoma and secondary hypercalcaemia. After induction chemotherapy the dog became severely hypocalcaemic and showed signs of weakness, muscle fasciculation and facial pruritus. Hormone analysis confirmed inadequate production of parathyroid hormone. Although hypocalcaemia has been previously reported as a component of tumour lysis syndrome, it has not been associated with transient parathyroid hormone deficiency.  相似文献   
9.
One in vitro and one in vivo metabolism experiment were conducted to examine the effects of supplemental Zn on ruminal parameters, digestion, and DMI by heifers fed low-quality prairie hay supplemented with urea. In Exp. 1, prairie hay was incubated in vitro for 24 h with five different concentrations of supplemental Zn (0, 5, 10, 15, and 20 ppm) and two concentrations of supplemental Mn (0 and 100 ppm), both provided as chloride salts. Added Mn increased (P < 0.02) IVDMD, but added Zn linearly decreased (P < 0.03) IVDMD. Added Zn tended to increase the amount of residual urea linearly (P < 0.06) at 120 min and quadratically (P < 0.02) at 180 min of incubation, although added Mn counteracted these effects of added Zn. Six 363-kg heifers in two simultaneous 3 x 3 Latin squares were fed prairie hay and dosed once daily via ruminal cannulas with urea (45 or 90 g/d) and with Zn chloride to provide the equivalent of an additional 30 (the dietary requirement), 250, or 470 ppm of dietary Zn. After a 7-d adaptation period, ruminal contents were sampled 2, 4, 6, 12, 18, 21, and 24 h after the supplement was dosed. Supplemental Zn did not alter prairie hay DMI (mean = 4.9 kg/d) or digestibility, although 470 ppm added Zn tended to decrease (P < 0.06) intake of digestible DM, primarily due to a trend for reduced digestibility with 470 ppm supplemental Zn. Zinc x time interactions were detected for both pH (P = 0.06) and NH3 (P = 0.06). At 2 h after dosing, ruminal pH and ruminal ammonia were linearly decreased (P < 0.05; P < 0.01) by added Zn. At 5 h after feeding, ruminal pH was linearly increased (P < 0.05) by added Zn, suggesting that added Zn delayed ammonia release from urea. The molar proportion of propionate in ruminal fluid was linearly and quadratically increased (P < 0.02; P < 0.01) whereas the acetate:propionate ratio was linearly and quadratically decreased (P = 0.02; P < 0.05) by added Zn. Through retarding ammonia release from urea and increasing the proportion of propionate in ruminal VFA, Zn supplementation at a concentration of 250 ppm may decrease the likelihood of urea toxicity and increase energetic efficiency of ruminal fermentation.  相似文献   
10.
A retrospective study was performed on skin samples from an outbreak of cutaneous papillomatosis in Merino sheep that occurred in 1995. The samples were processed for routine histology, electron microscopy and immunocytochemistry for papilloma viruses. Particles of approximately 55 nm diameter were found in some nuclei of the stratum granulosum cells, while immunocytochemistry gave positive staining of cell nuclei in this layer. This study confirms that papillomas associated with papillomaviruses occur in sheep in Patagonia.  相似文献   
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