Screening of an allelopathic potential in arbor species     

SAYAKA MORITA  MIHO ITO  JIRO HARADA 《Weed Biology and Management》2005,5(1):26-30

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Effects of soil moisture conditions on the emergence of weeds and rice plants from rainfed paddy soils in north‐east Thailand     

HIROHIKO MORITA  NOBUYUKI KABAKI 《Weed Biology and Management》2002,2(4):209-212

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Hematological and biochemical features of postpartum fever in the heavy draft mare     

Takahiro AOKI  Yuki KIMURA  Anna OYA  Akiko CHIBA  Mitsuo ISHII  Yasuo NAMBO 《Journal of Equine Science》2016,27(1):13-16

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Time-dependent changes in protein phosphorylation patterns in rat brain synaptosomes caused by deltamethrin     

Yoshiro Kanemoto  Essam E. Enan  Fumio Matsumura  Mitsuo Miyazawa 《Pest management science》1992,34(3):281-290

Effects of deltamethrin, a powerful pyrethroid insecticide, on the protein phosphorylation and dephosphorylation processes during depolarization in rat brain synaptosomes were studied by using [³²P]phosphoric acid as a starting radiotracer and high external concentration of potassium ions or veratridine (10^?-5 M) as depolarizing agents. At the onset of depolarization there was a quick rise in phosphorylation in various synaptic proteins for about 15–30 s followed by a gradual decline in levels of phosphorylation. The effect of deltamethrin (10^?-7 M) on this system was found to be dependent on the length of preincubation of the synaptosome with the pesticide prior to depolarization. At an early stage (0–3 min preincubation period) it caused a modest suppression of protein phosphorylation activities. When the period of deltamethrin preincubation was extended to 5–20 min, however, it caused a significant increase in protein phosphorylation throughout the depolarization period. At the later stage of the action of deltamethrin (e.g. preincubation period of 30–40 min), deltamethrin-treated synaptosomes no longer responded to the depolarization signal to raise the level of phosphorylation on many proteins. These results indicate that deltamethrin's actions on the synaptic process are complex. Depending on the length of exposure, its effects on protein phosphorylation responses in intact synaptosomes could be either stimulatory or inhibitory. To study the cause of deltamethrin-induced synaptic block at the later stage, effects of deltamethrin on protein kinases were studied by using lysed synaptic membranes with [gamma-³²P]ATP. Deltamethrin was shown to inhibit calcium–calmodulin-dependent protein phosphorylation activities at 10^?-7 M when given directly to the enzyme source 10 min prior to the addition of [³²P]ATP. Such an observation helps to explain the inhibitory action of deltamethrin on protein phosphorylation which occurs at the late stage of its action (i.e. preincubation time > 20 min).  相似文献   

Relation of Japanese <Emphasis Type="Italic">Xanthomonas campestris</Emphasis> pv. <Emphasis Type="Italic">vesicatoria</Emphasis> with worldwide strains revealed with three specific monoclonal antibodies     

Kenichi?TsuchiyaEmail author  Christophe C.M.?D’Ursel  Mitsuo?Horita  Yuzo?Nozu 《Journal of General Plant Pathology》2003,69(5):310-315

Strains of Xanthomonas campestris pv. vesicatoria Dye 1978 (Xcv), the causal agent of bacterial spot, have been classified into two groups based on their ability to hydrolyze starch. Three monoclonal antibodies (MAbs), 7AH10, 5HB3, and 4AD2, were produced immunized against the living bacteria and were specific to and could distinguish Xcv strains able or unable to hydrolyze starch (Amy⁺ or Amy^–). The MAb 7AH10, obtained against strain UPB141(Amy^–) reacted in an enzyme-linked immunosorbent assay with all the Amy^– strains (n = 19) and 1 of 11 Amy⁺ strains. Against Xcv 2625, an Amy^– unusual phenotype strain, MAb 5HB3, recognized 97% of our worldwide collection of Xcvs (n = 30). Also against that strain, the MAb 4AD2 reacted with none of the homologous Amy^– phenotypes and with 90% (n = 11) of the heterologous Amy⁺ phenotypes. For all the MAbs, cross reactions with other pathovars or species were less than 4% (n = 67). By assaying a Japanese collection of strains against the three MAbs, the Amy⁺ strains were distinguished from the Amy^– strains, and their relation with other world strains could be demonstrated. All the MAbs reacted with the lipopolysaccharide fraction of the bacterial cell wall during immunoblotting.  相似文献   

<Emphasis Type="Italic">Spongospora subterranea</Emphasis> soil contamination and its relationship to severity of powdery scab on potatoes     

Takato Nakayama  Mitsuo Horita  Tadayuki Shimanuki 《Journal of General Plant Pathology》2007,73(4):229-234

We investigated soil contamination by Spongospora subterranea f. sp. subterranea (Sss) and disease severity of powdery scab in 29 potato fields in Hokkaido, Japan, using a hydroponic culture method with tomato seedlings as bait plants. The quantity of Sss infection on the roots of bait plants was evaluated using the polymerase chain reaction (PCR) and expressed in terms of the infection potential in the soil. The infection potential was positively correlated with the disease severity of harvested tubers, whereas the spore ball density determined using PCR had an indistinct relationship with disease severity. The infection potential can be useful in evaluating soil contamination and in applying countermeasures against powdery scab.  相似文献   

A method for isolation of milled-wood lignin involving solvent swelling prior to enzyme treatment     

J. -Y. Chen  Yuichi Shimizu  Mitsuo Takai  Jusuke Hayashi 《Wood Science and Technology》1995,29(4):295-306

Summary A new lignin isolation method has been developed. Wood and pulp were subjected to ball milling, swelled in an organic solvent, and then treated with a cellulase. The enzyme digestion time could be shortened to 1 day with this method. The lignin obtained has been named Swelled Enzyme Lignin (SEL). Swelling and enzyme digestion conditions and their effects on lignins were investigated. The SEL's from wood could be directly washed with water, while those from pulp had to be washed with aqueous acetic acid because they were water soluble. The purification of crude SEL's was accomplished by extracting them with dioxane-water, and then precipitating and washing with ethyl ether. Lignin yields were 24–67% based on the total amount of lignin present. The characteristics of the SEL's were further investigated by gel-permeation chromatography (GPC), infrared and ¹³C nuclear magnetic resonance (NMR) spectroscopy.Symbols SUKP Softwood Unbleached Kraft Pulp - BUKP Birch Unbleached Kraft Pulp - TD Todomatsu (Abies sachalinensis) wood - MWL Milled Wood Lignin - MWLS (BK, TD) Milled Wood Lignin of SUKP (BUKP, TD) - SEL-C Swelled Enzyme Lignin (crude) - SELS-C SEL-C of SUKP, other materials named in the same way - SELS-90 SEL-C of SUKP and extracted with 90% dioxane-water - SELS-96 SEL-C of SUKP and extracted with 96% dioxane-water, other materials named in the same way  相似文献   

Condensation reactions of phenolic resins III: self-condensations of 2,4-dihydroxymethylphenol and 2,4,6-trihydroxymethylphenol (1)     

Naruyuki Kamo  Mitsuo Higuchi  Takehiro Yoshimatsu  Yukiko Ohara  Mitsuhiro Morita 《Journal of Wood Science》2002,48(6):491-496

This study reexamined the kinetics of the condensation reactions of hydroxymethylphenols with the purpose of elucidating the reaction mechanisms. This report discusses experimental results on the self-condensations of 2,4-dihydroxymethylphenols (2,4-DHMP) and 2,4,6-trihydroxymethylphenol (THMP), focusing on the order of reaction. The relations between the initial rates of reaction and the initial concentrations of reactants were investigated. Results quite different from those of previous reports were obtained. The order of reaction of the selfcondensation of 2,4-DHMP was found to be 1.1, which did not change with the alkali/2,4-DHMP molar ratio. The order of reaction of the self-condensation of THMP was found to vary with both the concentration of THMP and the alkali/THMP molar ratio. In the region of THMP concentrations above 1.5mol/l, the order of reaction was confirmed to be 2.0, which did not change with the alkali/THMP molar ratio. In the region of THMP concentrations below 1.0mol/l, the order of reaction varied with the alkali/THMP molar ratio, showing fractional numbers of 1.2–1.6. These results indicate that unimolecular reaction(s) and bimolecular reaction(s) take place simultaneously as the ratedetermining step in the condensation reactions of 2,4-DHMP and THMP and that the reaction mechanism changes with the species of reactant and, in some cases, with the reaction conditions.Part of this study was presented at the Wood Adhesives 2000 symposium, South Lake Tahoe, Nevada, USA, June 2000  相似文献   

Condensation reactions of phenolic resins V: cure-acceleration effects of propylene carbonate     

Naruyuki?Kamo  Hiroyuki?Okamura  Mitsuo?Higuchi  Mitsuhiro?MoritaEmail author 《Journal of Wood Science》2004,50(3):236-241

Propylene carbonate (PC) is known as one of the cure accelerators for alkaline phenol–formaldehyde resins. To elucidate the cure-acceleration mechanisms, the effects of PC on the condensation reactions of monomeric hydroxymethylphenols (HMPs) were investigated and compared with those of PC hydrolysate, sodium bicarbonate (NaHCO₃) and ethyl formate. Immediately after the reaction started, PC, decomposing itself simultaneously, accelerated the formation of the ortho-para methylene-bonded dimer of 2,4,6-trihydroxymethylphenol. This effect of PC was very similar to that of ethyl formate. To the contrary, PC hydrolysate accelerated the formation of the para-para methylene-bonded dimer throughout the course of the reaction. This effect of PC hydrolysate was identical to that of NaHCO₃. These results indicate that PC increases the reactivity of the ortho-hydroxymethyl group, presumably through transesterification. On the other hand, NaHCO₃ is formed by the hydrolysis of PC or decomposition of the transesterified HMPs and it increases the reactivity of the para-hydroxymethyl group.  相似文献   

Genetic analysis of fat‐to‐protein ratio,milk yield and somatic cell score of Holstein cows in Japan in the first three lactations by using a random regression model          下载免费PDF全文

Akiko Nishiura  Osamu Sasaki  Mitsuo Aihara  Hisato Takeda  Masahiro Satoh 《Animal Science Journal》2015,86(12):961-969

We estimated the genetic parameters of fat‐to‐protein ratio (FPR) and the genetic correlations between FPR and milk yield or somatic cell score in the first three lactations in dairy cows. Data included 3 079 517 test‐day records of 201 138 Holstein cows in Japan from 2006 to 2011. Genetic parameters were estimated with a multiple‐trait random regression model in which the records within and between parities were treated as separate traits. The phenotypic values of FPR increased soon after parturition and peaked at 10 to 20 days in milk, then decreased slowly in mid‐ and late lactation. Heritability estimates for FPR yielded moderate values. Genetic correlations of FPR among parities were low in early lactation. Genetic correlations between FPR and milk yield were positive and low in early lactation, but only in the first lactation. Genetic correlations between FPR and somatic cell score were positive in early lactation and decreased to become negative in mid‐ to late lactation. By using these results for genetic evaluation it should be possible to improve energy balance in dairy cows.  相似文献