Pathogenicity of porcine enterotoxigenic Escherichia coli that do not express K88, K99, F41, or 987P adhesins.     

T A Casey  B Nagy  H W Moon 《American journal of veterinary research》1992,53(9):1488-1492

Three-week-old weaned and colostrum-deprived neonatal (less than 1 day old) pigs were inoculated to determine the pathogenicity of 2 enterotoxigenic Escherichia coli isolates that do not express K88, K99, F41, or 987P adhesins (strains 2134 and 2171). Strains 2134 and 2171 were isolated from pigs that had diarrhea after weaning attributable to enterotoxigenic E coli infection. We found that both strains of E coli adhered in the ileum and caused diarrhea in pigs of both age groups. In control experiments, adherent bacteria were not seen in the ileum of pigs less than 1 day old or 3 weeks old that were noninoculated or inoculated with a nonpathogenic strain of E coli. These control pigs did not develop diarrhea. Antisera raised against strains 2134 and 2171 and absorbed with the autologous strain, grown at 18 C, were used for bacterial-agglutination and colony-immunoblot assays. Both absorbed antisera reacted with strains 2134 and 2171, but not with strains that express K99, F41, or 987P adhesins. A cross-reaction was observed with 2 wild-type K88 strains, but not with a K12 strain that expresses K88 pili. Indirect immunofluorescence with these absorbed antisera revealed adherent bacteria in frozen sections of ileum from pigs infected with either strain. We concluded that these strains are pathogenic and express a common surface antigen that may be a novel adhesin in E coli strains that cause diarrhea in weaned pigs.  相似文献   

Antiviral Effect of Saccharomyces cerevisiaeβ‐glucan to Swine Influenza Virus by Increased Production of Interferon‐γ and Nitric Oxide     

K. Jung  Y. Ha  S.‐K. Ha  D. U. Han  D.‐W. Kim  W. K. Moon  C. Chae 《Zoonoses and public health》2004,51(2):72-76

The aim of these experiments was to investigate the potential antiviral effect of Saccharomyces cerevisiaeβ‐glucan on the pneumonia induced by swine influenza virus (SIV). Forty colostrum‐deprived 5‐day‐old piglets were randomly divided into four groups of 10. The 20 pigs in groups 1 and 2 were administered Saccharomyces cerevisiaeβ‐glucan orally (50 mg/day/pig; En‐Bio Technology Co., Ltd) for 3 days before SIV infection and those in groups 3 and 4 were given culture medium/diluent alone. Groups 1 and 3 were inoculated intranasally with 3 ml of tissue culture fluid containing 2 × 10⁶ tissue culture infective doses 50% (TCID₅₀)/ml of SIV and those in groups 2 and 4 were exposed in the same manner to uninfected cell culture supernatant. The microscopic lung lesions induced by SIV infection (group 1 pigs) were significantly more severe than those induced by infection in animals pre‐administered β‐glucan (group 3) (P < 0.05). Significantly more SIV nucleic acid was detected in the lungs of pigs experimentally infected with SIV only (group 1) at 5, 7 and 10 days post‐inoculation (dpi) compared with lungs from pigs pre‐administered β‐glucan and infected with SIV (group 3) (P < 0.05). The concentrations of interferon‐γ (IFN‐γ) and nitric oxide (NO) in bronchoalveolar lavage fluid from pigs pre‐administered β‐glucan and infected with SIV (group 3) were significantly higher than for any other group at 7 and 10 dpi for IFN‐γ, and at 5, 7 and 10 dpi for NO (P < 0.05). Saccharomyces cerevisiaeβ‐glucan reduced the pulmonary lesion score and viral replication rate in SIV‐infected pigs. These findings support the potential application of β‐glucan as prophylactic/treatment agent in influenza virus infection.  相似文献   

Association mapping reveals loci associated with multiple traits that affect grain yield and adaptation in soft winter wheat     

Dennis N. Lozada  R. Esten Mason  Md Ali Babar  Brett F. Carver  Gina-Brown Guedira  Keith Merrill  Maria Nelly Arguello  Andrea Acuna  Lucas Vieira  Amanda Holder  Christopher Addison  David E. Moon  Randal G. Miller  Susanne Dreisigacker 《Euphytica》2017,213(9):222

Genome-wide association studies (GWAS) are useful to facilitate crop improvement via enhanced knowledge of marker-trait associations (MTA). A GWAS for grain yield (GY), yield components, and agronomic traits was conducted using a diverse panel of 239 soft red winter wheat (Triticum aestivum) genotypes evaluated across two growing seasons and eight site-years. Analysis of variance showed significant environment, genotype, and genotype-by-environment effects for GY and yield components. Narrow sense heritability of GY (h ²  = 0.48) was moderate compared to other traits including plant height (h ²  = 0.81) and kernel weight (h ²  = 0.77). There were 112 significant MTA (p < 0.0005) detected for eight measured traits using compressed mixed linear models and 5715 single nucleotide polymorphism markers. MTA for GY and agronomic traits coincided with previously reported QTL for winter and spring wheat. Highly significant MTA for GY showed an overall negative allelic effect for the minor allele, indicating selection against these alleles by breeders. Markers associated with multiple traits observed on chromosomes 1A, 2D, 3B, and 4B with positive minor effects serve as potential targets for marker assisted breeding to select for improvement of GY and related traits. Following marker validation, these multi-trait loci have the potential to be utilized for MAS to improve GY and adaptation of soft red winter wheat.  相似文献   

Thoracolumbar myelopathies in pug dogs     

Ian J. Wachowiak  Jon S. Patterson  Kathryn M. Winger  Kathleen L. Smiler  Robert Cole  Rachel Moon  Michael Kluz  Lisa R. Bartner 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2023,37(2):618-625

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Seasonal Effect on Zebu Embryo Quality as Determined by Their Degree of Apoptosis and Resistance to Cryopreservation     

YC Marquez  CS Galina  N Moreno  H Ruiz  A Ruiz  H Merchant 《Reproduction in domestic animals》2005,40(6):553-558

In order to optimize the production of embryos under tropical conditions and to test a possible seasonal effect on embryo quality, 40 Zebu cows were superovulated during the dry season (April to May) and during the rainy season (July to August). A total of 116 (average 2.7/cow) and 83 embryos (3.5 average/cow) were obtained during the respective seasons. After classification as good, fair or poor quality, embryos were tested based on their ultrastructural differences (n = 53 dry season 16 good, 20 fair and 17 poor and n = 61 rainy season 21 good, 20 fair and 20 poor) and their degree of apoptosis using the TUNEL technique (n = 30 during the dry season and n = 55 in the rainy season). Structural characteristics determining embryo quality varied between good and fair quality embryos. No difference, however, was observed between good, fair and poor quality embryos from the two seasons. The number of TUNEL-positive cells was different among embryos (p < 0.001), being lower in labelled cells of good quality embryos regardless of the season. Fewer apoptotic cells were observed in embryos assigned in all three quality levels during the rainy season (p < 0.001). Ultrastructural evaluations confirmed the results obtained by TUNEL. Cryopreserved embryos of good (n = 25 in each season) and fair quality (n = 11 dry season; n = 17 rainy season) showed a significant decrease of TUNEL-positive cells during the rainy season (p < 0.05). Results suggest that embryos collected in the dry season have more cellular damage in contrast; embryos cryopreserved in the rainy season appeared morphologically better equipped to result in a pregnancy following transfer.  相似文献   

An atlas of mammalian chromosomes. volume 8          下载免费PDF全文

Y. S. Moon 《Canadian journal of veterinary research》1975,39(4):473

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Epithelial cell dynamics in transmissible gastroenteritis of neonatal pigs     

D C Thake  H W Moon  G Lambert 《Veterinary pathology》1973,10(4):330-341

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THE USE OF IMIDOCARB DIPROPRIONATE FOR THE TREATMENT OF THEILERIA SERGENTI INFECTIONS OF CATTLE   总被引：1，自引：0，他引：1  

R. E. Purnell  Moon Chang Rae 《Australian veterinary journal》1981,57(5):224-226

SUMMARY Imidocarb diproprionate (IMDP) was used therapeutically at a dose of 1.2 mg IMDP/kg of body weight against Theileria sergenti infections in Hereford heifers on the island of Jeju-Do, Republic of Korea. In the first experiment IMDP was seen to be at least as effective in reducing T. sergenti parasitaemias as primaquine phosphate (primaquine), the drug normally used on the island. In the second and third experiments IMDP was shown to reduce T. sergenti parasitaemias by about 80%. No drug is known to be completely effective against T. sergenti infections but the suggestion is made that IMDP may be used in preference to primaquine for treatment of acutely ill animals since it also has the advantage of eliminating Babesia ovata, another element of the piroplasmosis syndrome on the island.  相似文献   

Transmission of porcine reproductive and respiratory syndrome virus by houseflies (Musca domestica)     

Otake S  Dee SA  Rossow KD  Moon RD  Trincado C  Pijoan C 《The Veterinary record》2003,152(3):73-76

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Genetic characterization of canine rotavirus isolated from a puppy in Korea and experimental reproduction of disease.   总被引：3，自引：0，他引：3  

Bo Kyu Kang  Dae Sub Song  Kwon Il Jung  Chul Seung Lee  Sung Jun Park  Jin Sik Oh  Dong Jun An  Jeong Sun Yang  Hyoung Joon Moon  Sang Sun Lee  Young Dhuk Yoon  Bong Kyun Park 《Journal of veterinary diagnostic investigation》2007,19(1):78-83

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