The role of ear environment in postharvest susceptibility of maize to toxigenic Aspergillus flavus     

Samuel K. Mutiga  Nelson Chepkwony  Owens A. Hoekenga  Sherry A. Flint‐Garcia  Rebecca J. Nelson 《Plant Breeding》2019,138(1):38-50

A kernel screening assay (KSA) was used to assess the genetic and environmental effects on the vulnerability of maize to aflatoxin accumulation. Kernels of 26 inbred lines that had been grown in seven environments, and 190 lines of the Intermated B73xMo17 (IBM) population grown in one location in the United States, were inoculated with a toxigenic strain of A. flavus and incubated in the dark at 30°C for 6 days. Percent kernel colonization (PKC), sporulation and aflatoxin were influenced by the maize genotypes (G), the location (“ear environment” or E) and the GxE interactions. Overall, low broad‐sense heritabilities were observed for PKC, sporulation and aflatoxin. PKC was significantly correlated with sporulation in all environments. Aflatoxin was positively correlated with colonization for two and with sporulation for all ear environments. Higher grain sulphur or magnesium in IBM was associated with less colonization or aflatoxin. Postharvest susceptibility of maize to aflatoxin is thus influenced by factors that are modulated by the ear environment. In a KSA, sporulation could be a proxy test for aflatoxin accumulation.  相似文献   

Neurological Manifestations of Niemann-Pick Disease Type C in Cats   总被引：3，自引：1，他引：2  

Karen R. Muñana  Patricia J. Luttgen  Mary Anna Thrall  Thomas W. Mitchell  David A. Wenger 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(2):117-121

Seven Domestic shorthair cats with a lysosomal storage disorder analogous to human Niemann-Pick disease type C, from a breeding colony were studied to characterize the neurological manifestations of this disorder. Affected cats were identified by means of liver biopsies at 4 to 6 weeks of age. Neurological examinations were performed at 2 week intervals from the onset of clinical signs. All cats displayed signs referrable to the cerebellum, with a subtle intention tremor noticed initially at 8 to 12 weeks of age; the disease was rapidly progressive. The tremor became more pronounced, menace response was lost, and severe dysmetria and ataxia developed. Three cats also had signs referrable to other areas of the central nervous system. Cats died or were euthanized between 12 and 43 weeks of age. Pathological findings included accumulation of substrate within neurons throughout the central nervous system, and axonal spheroid formation. The clinical and pathological findings in these cats are comparable to those in the human form of the disease.  相似文献   

Suspected Tension Pneumomediastinum In A Dog: A Case Report     

Elke Rudloff DVM  Dennis T. Crowe DVM  Jr. Rebecca Kirby DVM  Bobbie Mammato DVM  MPH 《Journal of Veterinary Emergency and Critical Care》1996,6(2):103-107

Successful resuscitation of a trauma-induced, suspected tension pneumomediastinum in a dog is presented. The cause was a caudal cervical penetration wound which acted as a one-way valve allowing air to enter, but not exit, the cranial mediastium. Early in the course of hospitalization, respiratory and cardiovascular collapse occurred; an emergent parasternotomy allowed decompression of the mediastinum, and the cradiovascular system immediately responded. The dog made a complete recovery following surgical exploration and removal of contaminated mediastinal tissue. Nine days of intensive care included 24-hour monitoring, oxygen therapy, intravenous crystalloids and colloids, continuous pleural suctioning, nutritional support, antibiotics, and pain control.  相似文献   

Recent advances in filtered-air positive-pressure (FAPP) housing for the production of disease-free chickens     

B W Mitchell  C W Beard  H W Yoder 《Avian diseases》1989,33(4):792-800

Design and performance information on a filtered-air positive-pressure (FAPP) housing system for disease-free poultry flocks is presented. The system includes many special features that result in excellent biological security, easy cleanup and maintenance, efficient control of environment, and a centralized alarm in the event of problems. The system has now housed eight flocks without any major problems. Based on its performance thus far, it should be useful as a reliable housing system for disease-free poultry.  相似文献   

DOES THE AMOUNT OF FOOD CONSUMED INFLUENCE THE GROWTH OF AN ANIMAL?     

Mitchell HH 《Science (New York, N.Y.)》1927,66(1720):596-600

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USE OF ULTRASOUND-GUIDED FINE-NEEDLE ASPIRATION BIOPSY AND AUTOMATED CORE BIOPSY FOR THE DIAGNOSIS OF GASTROINTESTINAL DISEASES IN SMALL ANIMALS     

Mitchell A.  Crystal  DVM  Dominique G.  Penninck  DVM  Michael E.  Matz  DVM  Susan H.  Pearson  BA  Greg O.  Freden  DVM  Richard M.  Jakowski  DVM 《Veterinary radiology & ultrasound》1993,34(6):438-444

The purpose of this study was to evaluate the usefulness of ultrasound-guided fine-needle aspiration biopsy and core biopsy in the diagnosis of infiltrative gastrointestinal diseases. Six dogs and seven cats with clinical signs of gastrointestinal disease underwent ultrasonography and intestinal lesions were identified. One or more ultrasound-guided fine-needle aspiration biopsy and/or core biopsy procedures were performed in each patient. Each patient also underwent one of the following additional procedures for comparison of results: 1) surgery (n=4), 2) endoscopy (n=2), 3) post mortem exam (n=3), and, 4) for lymphoma diagnosed with ultrasound-guided procedures, response to chemotherapy (n=4). Correct diagnoses were obtained in nine of the 13 patients, incorrect diagnoses in two of the 13 patients, and inconclusive diagnoses in two of the thirteen patients. Of the 16 total ultrasound-guided procedures performed, ten were confirmed as correct, three as incorrect, and three were non-diagnostic. Intestinal lesions with bowel wall thickness greater than 2.0 cm had a higher percentage of correct diagnoses than lesions of lesser wall thickness. Gastric lesions had a higher percentage of correct diagnoses than small and large intestinal lesions. Malignant lesions had a higher percentage of correct diagnoses than benign lesions. There were no complications. Ultrasound-guided gastrointestinal fine-needle aspiration biopsy and core biopsy appears to be a safe, accurate, and rapid procedure for use in the diagnosis of infiltrative gastrointestinal disease.  相似文献   

UV-B radiation and photosynthetic irradiance acclimate eggplant for outdoor exposure     

Latimer JG  Mitchell CA  Mitchell GA 《HortScience : a publication of the American Society for Horticultural Science》1987,22(3):426-429

Treatment of greenhouse-grown eggplant (Solanum melongena L. var. esculentum Nees. 'Burpee's Black Beauty') seedlings with supplemental photosynthetically active radiation from cool-white fluorescent lamps increased growth of plants subsequently transferred outdoors relative to growth of plants that received no supplemental radiation or were shaded to 45% of solar irradiation in the greenhouse before transfer outdoors. Eggplant seedlings transferred outdoors were placed under plastic tarps either to provide relative protection from solar ultraviolet-B (UV-B) radiation (280-315 nm) using Mylar film or to allow exposure to UV-B using cellulose acetate. Protection of seedlings from UV-B radiation resulted in greater leaf expansion than for UV-B-exposed seedlings, but no change in leaf or shoot dry weight occurred after 9 days of treatment. Specific leaf weight increased in response to UV-B exposure outdoors. Exposure of eggplant to UV-B radiation from fluorescent sunlamps in the greenhouse also decreased leaf expansion and leaf and shoot dry weight gain after 5 days of treatment. However, there were no differences in leaf or shoot dry weight relative to control plants after 12 days of UV-B treatment, indicating that UV-B treated plants had acclimated to the treatment and actually had caught up with non-UV-B-irradiated plants in terms of growth.  相似文献   

The veterinary practitioner's right to prescribe          下载免费PDF全文

Mitchell GA 《The Canadian veterinary journal. La revue veterinaire canadienne》1988,29(9):689-692

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Seismic stress effects on reproductive structures of tomato, potato, and marigold     

Akers SW  Mitchell CA 《HortScience : a publication of the American Society for Horticultural Science》1985,20(4):684-686

Periodic seismic (shaking) stress influenced the growth of sexual and asexual reproductive structures of three cultivated species. It delayed anthesis of Lycopersicon esculentum Mill. 'Patio' but not of Tagetes erecta L. 'Jubilee' or Solanum tuberosum L. 'Kennebec'. Shaken 'Jubilee' marigold plants produced the same number of flowers as undisturbed plants, but flowers of shaken plants were smaller. In contrast, seismic-stressed 'Patio' tomato produced fewer buds and flowers, but fruit set was enhanced relative to that of controls. Seismic stress also reduced tuber weight of 'Kennebec' potato, whereas tuber number was unaffected. The overall effect of seismic stress was to reduce the growth of reproductive structures and, in some cases, the number of reproductive structures that developed.  相似文献   

Identification of tissue hypoxia in the livers of ascitic and hypoxia‐induced broilers using trypan blue     

M. H. Maxwell  I. A. Alexander  G. W. Robertson  M. A. Mitchell  C. C. McCorquodale 《British poultry science》1995,36(5):791-798

1. The perfusion of livers with the vital dye trypan blue was performed to test for evidence of tissue hypoxia in 3 groups of young broiler chickens, namely, ascitic, hypoxia‐induced and controls.

2. Hepatocytes that stained with trypan blue were considered to be dead or dying before fixation and represented damaged cells.

3. The proportion of trypan blue‐stained hepatocytes in the livers of ascitic birds was slightly less than half that observed in the hypoxia‐induced birds but significandy more than the proportion of stained cells observed in control birds.

4. Liver damage in the ascitic birds was also assessed biochemically by an altered enzyme profile.

5. The study demonstrated that increased trypan blue uptake in the livers of ascitic birds reared at sea‐level may be the consequence of hypoxia stress caused by reduced oxygen utilisation.  相似文献