Indirect solid-phase microradioimmunoassay for detection of pseudorabies virus antibody in swine sera.     

C L Kelling  W L Staudinger  M B Rhodes 《American journal of veterinary research》1978,39(12):1955-1957

An indirect solid-phase microradioimmunoassay (IRIA) was developed for detection and quantitation of antibodies to pseudorabies virus (PRV) in swine serum. Qualitative results of the IRIA compared closely with results of the serum neutralization test (NT) and the microimmunodiffusion test (MIDT). The IRIA was more sensitive than the NT for detection of antibodies to PRV in swine serum. The IRIA result is expressed numerically. With the IRIA and NT, antibody to PRV was first detectable in 3 experimentally infected pigs at 9 days after inoculation. With MIDT, antibody was detected in the 3 experimentally infected pigs at 9 days after inoculation. With the MIDT, antibody was detected in the 3 experimentally infected pigs at 7, 8, and 9 days after inoculation. The IRIA results are obtainable within a few hours; the NT and MIDT require 48 hours for completion.  相似文献   

High night-time temperature during flowering and pod filling affects flower opening,yield and seed fatty acid composition in canola     

Meghnath Pokharel  Anuj Chiluwal  Michael Stamm  Doohong Min  Davina Rhodes  S.V. Krishna Jagadish 《Journal of Agronomy and Crop Science》2020,206(5):579-596

Winter canola (Brassica napus L.) is highly sensitive to increasing temperatures during the reproductive and pod-filling stages. Although the impact of high day-time temperature stress on yield and quality has been documented in canola, similar information under high night-time temperature (HNT) stress is not available. Using six hybrids and four open-pollinated cultivars, we observed a marked shift in peak flowering towards earlier, cooler hours of the morning under HNT. Averaged across two independent experiments, the photochemical efficiency of photosystem II was significantly decreased (3%), with a significant increase in thylakoid membrane damage (13%) in the leaves of susceptible cultivars under HNT stress. Similarly, the susceptible cultivars also recorded significant reduction in biomass (34%), pod number (22%), pod weight (37%) and total seed weight (40%) per plant while the same set of agronomic traits were not affected among the tolerant cultivars. Quantitative impact of heat stress was confirmed with increased sensitivity to HNT exposure from gametogenesis until maturity resulting in a significantly higher yield loss compared to stress exposure from post-flowering till maturity. HNT significantly decreased oil concentration, but increased protein concentration and saturated fatty acid levels in seeds of the susceptible cultivars. However, HNT had no impact on the unsaturated fatty acids in both hybrids and the open-pollinated cultivars. Breeding targets based on fatty acid composition for enhancing canola seed quality may not be easily amenable due to the inconsistency documented with the compositional changes under heat stress. In summary, our findings conclude that canola hybrids are better suited to regions experiencing heat stress, compared to open-pollinated cultivars, indicating the possibility of a complete shift to hybrid canola cultivation under predicted hotter climates in the future.  相似文献   

Serum antibody responses to vaccinal antigens in lean and obese geriatric dogs     

John Ellis  Sheryl Gow  Carrie Rhodes  Stacey Lacoste  Lyndsay Kong  Kristyna Musil  Elisabeth Snead 《The Canadian veterinary journal. La revue veterinaire canadienne》2016,57(5):531-534

The immune responses in control dogs [1 to 4 years of age, body condition score (BCS): 4 to 5 out of 9] were compared to those of aging dogs (based on breed and body size) either categorized as lean (BCS: 4 to 5 out of 9) or obese (BCS: 8 to 9 out of 9). Of interest were the serum titers to the following common agents found in vaccines, canine parainfluenza virus (CPIV), canine parvovirus (CPV), canine distemper virus (CDV), canine respiratory coronavirus (CRCoV), and Bordetella bronchiseptica. There were no statistical differences in the antibodies to CPIV, B. bronchispetica, and CRCoV, among the age/weight categories, nor among the age/weight categories and the time, in days, between the date of sample collection and the date of the last recorded vaccination for CPIV, B. bronchiseptica, CPV, and CDV. For CPV, the control dogs had significantly (P < 0.002) higher serum neutralization (SN) titers than the lean geriatric dogs and the obese geriatric dogs. For CDV SN titers, the only statistically significant (P = 0.01) difference was that the control dogs had higher SN titers than the lean geriatric dogs.  相似文献   

Retrospective evaluation of factors associated with emergency frequency and survival in equids presenting for emergency care (2019–2020): 3071 cases     

Jessica Bouton DVM  Langdon Fielding DVM  DACVECC  DACVSMR  Dustin Major DVM  DACVS  Emma Deane DVM  DACVECC  Jennifer Mayer VMD  DACVECC  Diane Rhodes DVM  DACVIM 《Journal of Veterinary Emergency and Critical Care》2023,33(5):598-605

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Treatment of anovulatory anoestrous postpartum dairy cows with a gonadotropin-releasing hormone (GnRH), prostaglandin F2 alpha, GnRH regimen or with progesterone and oestradiol benzoate     

McDougall S  Cullum AA  Annis FM  Rhodes FM 《New Zealand veterinary journal》2001,49(5):168-172

AIM: To compare 2 treatments for anovulatory anoestrus (AA) in postpartum dairy cows. The treatments were combinations of gonadotropin-releasing hormone (GnRH) and prostaglandin F2 (PG) or progesterone (P4) and oestradiol benzoate (ODB). METHODS: Forty AA cows from each of 5 herds were blocked by age (2 or 2 years old) and randomly assigned to 1 of 2 treatments. The first group (GPG) were treated with 250 mug of a GnRH analogue, gonadorelin, followed 7 days later by 15 mg of the PG analogue, luprostiol. Two days later the cows were injected with 250 mug of gonadorelin. Cows were artificially inseminated 16-24 h after the second GnRH injection. The second group (P4+ODB) were treated with an intravaginal P4 releasing device for 6 days, followed 24 h after device removal by injection of 1 mg of ODB. Cows were pregnancy tested 35-40 days after the initial insemination and twice again at 6-8 week intervals thereafter. RESULTS: There was no significant difference between P4+ODB and GPG groups in the percentage of cows submitted for insemination in the first 7 days (94.0% vs 100% for P4+ODB vs GPG, respectively; p>0.3), in conception rate to first insemination within the first 7 days (43.6% vs 35.0% for P4+ODB vs GPG, respectively; p>0.2), in the percentage of cows conceiving in the first 28 days of the breeding period (68.0% vs 58.3%, P4+ODB vs GPG, respectively; p>0.1), or in median interval from the end of treatment to conception (20 vs 21 days; p>0.1). CONCLUSIONS: No differences in the reproductive performance of AA cows treated with either P4+ODB or GPG were detected. However, given the small number of animals enrolled, further data are required before the GPG protocol can be recommended for treatment of AA cows.  相似文献   

Detection of a corpus luteum in apparently anoestrous cows by manual palpation, transrectal ultrasonography and plasma progesterone concentration     

McDougall S  Rhodes FM 《New Zealand veterinary journal》1999,47(2):47-52

AIMS: To determine the level of agreement between transrectal ultrasonography, manual palpation and measurement of progesterone in the detection of corpora lutea in post-partum cows. To evaluate the reproductive performance of cows not detected in oestrus prior to mating, but detected with a corpus luteum at the start of mating. METHODS: Cows from seven herds which were not detected in oestrus were examined between 5 and 7 days before the herd's planned start of mating. Ovaries were examined by palpation and ultrasonography in 160 cows and plasma progesterone concentrations were also measured in 103 cows. Insemination dates and pregnancy data were recorded in 159 cows not detected in oestrus but having a corpus luteum and 1405 cycling herd mates. RESULTS: The level of agreement between ultrasonography and measurement of progesterone (kappa = 0.74) was higher than with manual palpation (kappa = 0.67) although it was significant for both techniques (p <0.001). Cows which were not detected in oestrus, but detected with a corpus luteum had a longer interval to first service (12 v. 10 days, p <0.05) and conception (19 v. 15 days, p = 0.01) than cycling herd mates and had a higher empty rate (9.7% v. 3.8%, p <0.001). CONCLUSIONS: These data indicate that ultrasonography may provide a better diagnostic tool for examining cows not detected in oestrus than manual palpation or measurement of progesterone concentration. Cows not detected in oestrus but having a corpus luteum were shown to have significantly poorer reproductive performance than cycling herd mates.  相似文献   

Letter: Use of isoniazid in cattle.          下载免费PDF全文

T C Watts  S M Olson    C S Rhodes 《The Canadian veterinary journal. La revue veterinaire canadienne》1974,15(2):28

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Angiograms and Angiocardiograms in Dogs and Cats: Some Unusual Filling Defects1     

F. K. Bohn  W. H. Rhodes 《Veterinary radiology & ultrasound》1970,11(1):21-28

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Grateful for article analyzing women in veterinary medicine     

Rhodes L 《Journal of the American Veterinary Medical Association》2002,220(12):1778

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PRODUCTIVITY AND CANOPY STRUCTURE OF TWO CONTRASTING VARIETIES OF PERENNIAL RYEGRASS (LOLIUM PERENNE L.) GROWN IN A CONTROLLED ENVIRONMENT     

I. Rhodes 《Grass and Forage Science》1971,26(1):9-12

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