全文获取类型
收费全文 | 84篇 |
免费 | 3篇 |
专业分类
林业 | 1篇 |
10篇 | |
综合类 | 22篇 |
农作物 | 2篇 |
水产渔业 | 16篇 |
畜牧兽医 | 34篇 |
园艺 | 2篇 |
出版年
2021年 | 2篇 |
2020年 | 1篇 |
2019年 | 2篇 |
2018年 | 1篇 |
2016年 | 2篇 |
2015年 | 1篇 |
2014年 | 2篇 |
2013年 | 1篇 |
2011年 | 5篇 |
2010年 | 4篇 |
2009年 | 6篇 |
2008年 | 5篇 |
2007年 | 2篇 |
2006年 | 6篇 |
2005年 | 1篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 4篇 |
2001年 | 3篇 |
2000年 | 5篇 |
1999年 | 5篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1992年 | 1篇 |
1989年 | 4篇 |
1988年 | 1篇 |
1984年 | 1篇 |
1983年 | 1篇 |
1980年 | 1篇 |
1977年 | 1篇 |
1976年 | 1篇 |
1975年 | 1篇 |
1974年 | 1篇 |
1970年 | 1篇 |
1945年 | 1篇 |
1943年 | 1篇 |
1942年 | 1篇 |
1941年 | 1篇 |
1938年 | 1篇 |
1927年 | 1篇 |
1911年 | 1篇 |
排序方式: 共有87条查询结果,搜索用时 812 毫秒
1.
A urinary bladder mass in a 12-year-old spayed female West Highland White Terrier was diagnosed after exploratory surgery and biopsy as a transitional cell carcinoma. Four months later the dog presented with an ulcerated plaque-like cutaneous lesion at the previous surgical incision site; concurrent inguinal lymphadenopathy and recurrence of the urinary bladder mass were identified. Transitional cell carcinoma was diagnosed at all 3 sites. Although a definitive relationship cannot be established between the initial surgery for urinary bladder mass and the resultant subcutaneous lesion, surgical implantation should be considered as a source for the neoplastic cells. 相似文献
2.
Effect of vaccination on serum concentrations of total and antigen-specific immunoglobulin E in dogs
HogenEsch H Dunham AD Scott-Moncrieff C Glickman LT DeBoer DJ 《American journal of veterinary research》2002,63(4):611-616
OBJECTIVE: To determine the effect of vaccination on serum concentrations of total and antigen-specific IgE in dogs. ANIMALS: 20 female Beagles. PROCEDURE: Groups of 5 dogs each were vaccinated repeatedly between 8 weeks and 4 years of age with a multivalent and rabies vaccine, a multivalent vaccine only, or a rabies vaccine only. A fourth group of 5 dogs served as unvaccinated controls. Serum concentrations of total immunoglobulins and antigen-specific IgE were determined following vaccination. RESULTS:-The multivalent vaccine had little effect on serum total IgE concentrations. The concentration of IgE increased slightly following vaccination for rabies at 16 weeks and 1 year of age and increased greatly after vaccination at 2 and 3 years of age in most dogs, with a distinct variation between individual dogs. Vaccination had no effect on serum concentrations of IgA, IgG, and IgM as measured at 2 and 3 years of age. The rabies vaccine contained aluminum adjuvant in contrast to the multivalent vaccine. An increase of IgE that was reactive with vaccine antigens, including bovine serum albumin and bovine fibronectin, was detected in some of the dogs vaccinated for rabies. There was no significant correlation between serum concentrations of total IgE and antigen-specific IgE following vaccination. Serum total IgE concentration rapidly returned to preimmunization concentrations in most dogs, but high concentrations of antigen-specific IgE persisted. CONCLUSIONS AND CLINICAL RELEVANCE: Vaccination of dogs for rabies increases serum concentrations of total IgE and induces IgE specific for vaccine antigens, including tissue culture residues. Vaccination history should be considered in the interpretation of serum total IgE concentrations. 相似文献
3.
Spatial and temporal landscape patterns have long been recognized to influence biological processes, but these processes often
operate at scales that are difficult to study by conventional means. Inferences from genetic markers can overcome some of
these limitations. We used a landscape genetics approach to test hypotheses concerning landscape processes influencing the
demography of Lahontan cutthroat trout in a complex stream network in the Great Basin desert of the western US. Predictions
were tested with population- and individual-based analyses of microsatellite DNA variation, reflecting patterns of dispersal,
population stability, and local effective population sizes. Complementary genetic inferences suggested samples from migratory
corridors housed a mixture of fish from tributaries, as predicted based on assumed migratory life histories in those habitats.
Also as predicted, populations presumed to have greater proportions of migratory fish or from physically connected, large,
or high quality habitats had higher genetic variability and reduced genetic differentiation from other populations. Populations
thought to contain largely non-migratory individuals generally showed the opposite pattern, suggesting behavioral isolation.
Estimated effective sizes were small, and we identified significant and severe genetic bottlenecks in several populations
that were isolated, recently founded, or that inhabit streams that desiccate frequently. Overall, this work suggested that
Lahontan cutthroat trout populations in stream networks are affected by a combination of landscape and metapopulation processes.
Results also demonstrated that genetic patterns can reveal unexpected processes, even within a system that is well studied
from a conventional ecological perspective. 相似文献
4.
W W Miller J S Wilkins L L Dunham 《Journal of the Association of Official Analytical Chemists》1975,58(1):10-18
Residues of isopropyl (2E,4E)-11-methoxy-3,7,11-trimethyl-2,4-dodecadienoate (Altosid) insect growth regulator are determined in waters, soils, plants, milk, eggs, fish, shellfish, poultry and cattle tissues, blood, urine, and feces. Acetonitrile is the primary extraction solvent for all samples. Residues are extracted by high-speed blending followed by vacuum filtration. Fatty extracts are subjected to cold-temperature precipitation and filtration. Samples are cleaned up by petroleum ether partitioning and Florisil and neutral alumina chromatography. The concentrated eluants are analyzed by gas-liquid chromatography (GLC) on columns of differing polarity, using hydrogen flame ionization detectors. The identity of suspected residues is confirmed by additional GLC and by mass fragmentography. The lower limits of detection were: water samples, 0.0004-0.001 ppm; soils, blood, and urine, 0.001 ppm; forage grasses, forage legumes, and rice foliage, 0.005 ppm; and milk, eggs, fish, shellfish, poultry and cattle tissues, and feces, 0.010 ppm. 相似文献
5.
Putative nonautonomous transposable elements from channel catfish (Ictalurus punctatus) were identified. They were named Tipnon elements for Tc1-like transposable elements from channel catfish that are nonautonomous. These elements were defined by their terminal repeats that share identity to members of the known Tc1/mariner transposon superfamily. They show structural similarities to Tc1-like elements, but share little sequence identity beyond the terminal inverted repeats. They do not harbor any amino acid blocks that show similarities to the Tc1-like or other transposases and thus may represent truly nonautonomous transposons in channel catfish. They are abundant in the channel catfish genome with a copy number of 32000, having 500 base pair per copy, this family of nonautonomous transposon-like elements account for 1.6% of the channel catfish genomic DNA. Their high abundance and transposon-like terminal repeats indicate that they may play important roles in gene evolution and in genomic architecture of catfish. Similarity search for potential coding capacity of the Tipnon elements revealed that they contain sequence blocks that can potentially encode amino acid blocks similar to the para-type sodium channel proteins in cockroaches or house flies, proteins that function in the central nervous system as voltage-gated sodium transporters. Sequences surrounding the terminal inverted repeats are divergent from those used by the reconstructed Sleeping Beauty fish transposase. 相似文献
6.
7.
8.
R.J. Dunham 《Soil & Tillage Research》1983,3(2):123-133
A traditional cultivation technique for cereals in the West African Savanna is described. Measurements of soil moisture showed that at the start of the rainy season when rainfall is erratic, the technique assists an early-planted crop to survive dry spells by providing the maximum amount of available water close to the planting position. This is achieved by the ingenious device of making planting hills in the furrows between existing ridges. The essential features of this hill-in-furrow technique could be retained advantageously in mechanised cultivation systems. 相似文献
9.
Cytotoxic T lymphocytes (CTL) against mouse P815 cells were detected after stimulation of porcine peripheral blood mononuclear cells (PBMC) with irradiated Balb/c splenocytes. In vivo priming prior to in vitro stimulation slightly enhanced CTL activity, but lysis of targets was undetectable from lymphocytes from non-immune or immune animals that were not cultured with mouse splenocytes. After primary culture with Balb/c (H-2d) splenocytes, specific killing of P815 (H-2d) targets and not L929 (H-2k) targets indicated that recognition was specific for the H-2 locus. Similarly, CTL primed by mouse cells from either of two congenic strains recognized targets with alleles homologous to the stimulating cells. The anti-murine CTL was confirmed to be a CD8+ T cell based on studies using specific monoclonal antibodies to the porcine CD4 or CD8 cells. The cells responsible for the cytotoxicity of P815 targets lacked the characteristics of non-specific NK cells because (1) naive PBMC were unable to lyse NK targets (K562 cells) during the 4 h cytotoxic assay and (2) CTL killing of P815 targets increased with time after primary stimulation, whereas killing of K562 cells remained low at all times. These results suggest that porcine CTL can be readily generated against the xenogeneic mouse major histocompatibility complex. 相似文献
10.