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1.
The primate fauna of South Africa has historically been viewed as comprising three diurnal cercopithecoid taxa – chacma baboons (Papio ursinus), vervet (Chlorocebus pygerythrus) and samango monkeys (Cercopithecus albogularis) – and two nocturnal lorisoid species – the thick-tailed greater galago (Otolemur crassicaudatus) and the southern lesser galago (Galago moholi). Here we report the positive identification of a third galago species within South Africa’s borders: the Mozambique dwarf galago or Grant’s galago, Galagoides granti (Thomas and Wroughton, 1907). The taxon was previously held to be restricted to Mozambique, eastern Zimbabwe, Malawi and Tanzania, but we have also observed it in the sand forest of Tembe Elephant Park and the Tshanini Community Reserve, near the Mozambique border. The species was formerly mistaken for Galago moholi, erroneously (we believe) extending the range of the latter species into northern KwaZulu-Natal. In South Africa the two small galagos are unlikely to have overlapping ranges: Galago moholi prefers dry savanna woodlands, whereas Galagoides granti is apparently confined to dry sand forest. However, both species may coexist with the larger and more widespread Otolemur crassicaudatus, an inhabitant of moist savanna, forest edge and thicket. The true South African ranges of both small galago species need to be ascertained.  相似文献   
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The present study aimed to determine the most efficient experimental conditions of iron sulfate use leading to optimal inhibition in the development of fungal pathogens. Assays have been focused on fungal species inducing severe grapevine diseases. FeSO4 directly inhibited the in vitro mycelial growth of Botrytis cinerea, Eutypa lata, Phaeomoniella chlamydospora, Phaeoacremonium aleophilum, Diplodia seriata, and Neofusicoccum parvum with variable efficiency in the range of 0.5–10 mM. The development was always completely inhibited at 20 mM. This inhibitory effect was greatly increased at acidic pH values. The anionic moiety of the molecule was of importance since bromide, chloride and sulfate were highly active, whereas acetate and oxalate showed a small effect. Electron microscope observations on E. lata and B. cinerea showed that a treatment with FeSO4 induced dramatic changes in the hyphal organization leading to cell death. No toxicity was observed on grapevine leaves following repeated FeSO4 sprays in the antifungal concentration range. Therefore, FeSO4 may be proposed to effectively replace the long-term pollutant use of CuSO4 as an antifungal agent, with the additional advantage of iron being an important plant micronutrient.  相似文献   
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In Central Europe vast wetland areas have been converted into agricultural land over the past few centuries. Long-term spatially explicit reconstructions of wetland cover changes at regional scale are rare but such information is vital for setting appropriate wetland conservation and restoration goals. In this study wetland cover change over the past 150 years was analyzed for the Canton Zurich (Switzerland) using information from historical and current topographical maps. Mapping instructions changed significantly over time, i.e., wetlands were mapped more conservatively on older maps. Therefore a technique was developed to account for changes in mapping instructions and to reconstruct a series of comparable maps spanning 1850–2000. Wetland cover dramatically decreased from 13,759 ha in 1850 (more than 8% of the total study area) to 1,233 ha in 2000 (less than 1%). Largest loss is observed for the first half of the twentieth century when more than 50% of the total wetland loss occurred. In 1850, almost all wetland patches were connected in two large networks defined by a 500 m buffer around all wetland patches to account for typical dispersal distances of wetland animals. Despite extensive wetland loss, this networks remained largely intact until 1950, but then collapsed into many medium and small networks consisting of only few wetland patches. In addition to the direct loss of wetland habitats increased habitat fragmentation is limiting metapopulation dynamics and hindering genetic exchange between populations. Amphibians and other wetland animals are particularly prone to habitat fragmentation because of their limited migration abilities. This may lead to time-delayed extinction in the future because current species occurrence might rather reflect historical than current wetland cover and habitat configuration. Future restoration efforts should focus on reestablishing connectivity between remaining smaller wetland networks.  相似文献   
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Green labeled pectins were extracted by an environmentally friendly way using proteases and cellulases being able to act on proteins and cellulose present in cell walls. Pectins were isolated from different plant byproducts, i.e., chicory roots, citrus peel, cauliflower florets and leaves, endive, and sugar beet pulps. Enzymatic extraction was performed at 50 degrees C for 4 h, in order to fulfill the conditions required for microbiological safety of extracted products. High methoxy (HM) pectins of high molar mass were extracted with three different enzyme mixtures. These pectins were subsequently demethylated with two pectin methyl esterases (PMEs), either the fungal PME from Aspergillus aculeatus or the orange PME. It was further demonstrated that high molar mass low methoxy (LM) pectins could also be extracted directly from cell walls by adding the fungal PME to the mixture of protease and cellulase. Moreover, health benefit pectic oligosaccharides, the so-called modified hairy regions, were obtained after enzymatic treatment of the residue recovered after pectin extraction. The enzymatic method demonstrates that it is possible to convert vegetable byproducts into high-added value compounds, such as pectins and pectic oligosaccharides, and thus considerably reduce the amount of these residues generated by food industries.  相似文献   
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The aim of this study was to investigate the cause of variation in the digestibility of pea protein in poultry and to find a tool to select genotypes with high digestibility potential by using an in vitro hydrolysis assay. Eight pea genotypes were selected for their difference in seed protein content and composition. To reduce the variation due to tannins and particle size, seeds from these 8 genotypes were dehulled and micro-ground. They were incorporated as the only protein source in 8 different experimental isoproteinaceous diets with similar metabolisable energy content. The amino acid digestibility was studied in cecectomized chickens. A balance method was used to obtain apparent digestibility, and the isotope dilution technique was used to determine endogenous losses and true digestibility, after feeding a double labelled test meal containing chromic oxide and 15N-labelled peas. The 8 diets showed differences in apparent amino acid digestibility. The average apparent digestibility for all amino acids varied between 79.5 and 86.3%, with the highest values for arginine (85.2 to 90.8%) and glutamic acid (85.2 to 90.5%), and the lowest values for cystine (63.3 to 69.7%) and tryptophan (69.1 to 80.3%). This variability of apparent amino acid digestibility was due to variations in endogenous losses and true digestibility among the 8 pea genotypes. The average endogenous losses as determined for 9 amino acids ranged from 3.6 to 5.4% of ingested amino acids, with the highest value for threonine (8.0 to 11.0%). The average true digestibility varied between 84.4 and 90.2%, with the highest values for lysine (89.0 to 95.0%), and the lowest for isoleucine (81.0 to 88.7%) and valine (82.4 to 88.7%). In vitro hydrolysis of protein from micro-ground seeds was performed for the 8 pea genotypes using three proteases (pepsin, trypsin and chymotrypsin). The quantity of small peptides (< 3 kDa) that appeared after the combined hydrolysis with pepsin (3 h) followed by trypsin and chymotrypsin (15 min) was significantly correlated with the average true digestibility of the 8 genotypes (R = 0.74; P < 0.05).  相似文献   
8.
As the various components of the cadmium (Cd) root sink have not been clearly described, there is a need to precisely measure the respective contributions of apoplast and symplast to short‐term root Cd uptake and to explain the linear component of the absorption isotherms. A new method of fractionating Cd in roots was applied to two plant species with contrasting abilities to accumulate Cd: maize (Zea mays) and a Cd‐hyperaccumulating ecotype of alpine pennycress (Noccaea caerulescens). Their roots were exposed for 1 h to increasing concentrations of labeled Cd. Series of desorption baths were used to obtain the root apoplastic Cd in combination with a brief freezing step in liquid nitrogen to separate the intracellular metal from the apoplastic one. The apoplastic uptake accounted for 15% to 82% and for 48% to 96% of the total Cd uptake of maize and of alpine pennycress roots, respectively. In the case of maize, the concentration‐dependent symplastic net flux fitted a biphasic Michaelis‐Menten function, while in the case of alpine pennycress, a Michaelis‐Menten‐plus‐linear function proved a better fit. The second component of the symplastic net flux may reflect absorption through a low‐affinity transport system. Short‐term Cd uptake by roots is dominated by the high‐affinity transport system for exposure concentrations below 1 μM for maize and 0.2 μM for alpine pennycress, while cell‐wall binding prevailed for higher exposure concentrations.  相似文献   
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The effects of fruit and vegetable extract (Oxxynea) on plasma cholesterol, early atherosclerosis, cardiac production of superoxide anion, and NAD(P)H oxidase expression were studied in an animal model of atherosclerosis. Thirty six hamsters were divided into two groups of 18 and fed an atherogenic diet for 12 weeks. They received by gavage either water or Oxxynea in water at a human dose equivalent of 10 fruits and vegetables per day. Oxxynea lowered plasma cholesterol and non-HDL cholesterol, but not HDL-cholesterol, and increased plasma antioxidant capacity. It also strongly reduced the area of aortic fatty streak deposition by 77%, cardiac production of superoxide anion by 45%, and p22phox subunit of NAD(P)H oxidase expression by 59%. These findings support the view that chronic consumption of antioxidants supplied by fruits and vegetables has potential beneficial effects with respect to the development of atherosclerosis. The underlying mechanism is related mainly to inhibiting pro-oxidant factors and improving the serum lipid profile.  相似文献   
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