Experimental infection of pregnant gilts with swine hepatitis E virus   总被引：1，自引：0，他引：1       下载免费PDF全文

Chaiyan Kasorndorkbua  Brad J. Thacker  Patrick G. Halbur  Denis K. Guenette  Ryan M. Buitenwerf  Ryan L. Royer    Xiang-Jin Meng 《Canadian journal of veterinary research》2003,67(4):303-306

To determine the effect of swine hepatitis E virus (HEV) infection on pregnant gilts, their fetuses, and offspring, 12 gilts were intravenously inoculated with swine HEV. Six gilts, who were not inoculated, served as controls. All inoculated gilts became actively infected and shed HEV in feces, but vertical transmission was not detected in the fetuses. There was no evidence of clinical disease in the gilts or their offspring. Mild multifocal lymphohistiocytic hepatitis was observed in 4 of 12 inoculated gilts. There was no significant effect of swine HEV on fetal size, fetal viability, or offspring birth weight or weight gain. The offspring acquired anti-HEV colostral antibodies but remained seronegative after the antibodies waned by 71 days of age. Swine HEV infection induced subclinical hepatitis in pregnant gilts, but had no effect on the gilts' reproductive performance, or the fetuses or offspring. Fulminant hepatitis associated with HEV infection was not reproduced in gilts.  相似文献   

Influence of intensive feeding on reproductive performance of Gobra zebus at the Dahra Center of Zootechnical Research]     

J P Denis  A I Thiongane 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1978,31(1):85-90

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Integrated Pest Management in Cocoa Agroforests in Southern Cameroon: Constraints and Overview     

Denis J. Sonwa  Ousmane Coulibaly  A. Akinwumi Adesina  Stephan F. Weise  Mathurin Tchatat 《Integrated Pest Management Reviews》2002,7(3):191-199

Imports of pesticides have long been subsidised for use in the cocoa agroforests of the humid forest zone of southern Cameroon. With the liberalisation of the cocoa and pesticides sectors and the devaluation of the local currency (CFA franc), farmers are facing fluctuations in the price paid for cocoa and the high cost of farm inputs. Without the support of the extension services, they themselves have developed traditional integrated control methods based on the use of plant extracts mixed with conventional pesticides. From a survey of 300 cocoa farmers, the study assesses the farmers' command of these methods, the pests controlled, the problems encountered and the institutional constraints in the definition and dissemination of integrated control methods. The study concludes with recommendations for research and development towards the better definition and dissemination of integrated control methods. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Molecular detection of sugarcane striate virus and sugarcane white streak virus and their prevalence in the Miami World Collection of sugarcane and related grasses     

Wardatou Boukari  Denis Filloux  Jean Heinrich Daugrois  Emmanuel Fernandez  Dimitre Mollov  Claudia Kaye  Martha Hincapie  Alexander Sanchez  Jianping Wang  Philippe Roumagnac  Philippe Rott 《Plant pathology》2020,69(6):1060-1069

Viruses in the genus Mastrevirus (family Geminiviridae), including those infecting sugarcane, have natural geographical ranges almost exclusively restricted to Africa and the Indian Ocean islands off the African coast. Only sugarcane white streak virus (SWSV) in Barbados and sugarcane striate virus (SStrV) in Florida and Guadeloupe are known to infect a few sugarcane varieties in the Western Hemisphere. In this study, PCR assays were developed to detect these two viruses in sugarcane. Five hundred and seventy-one DNA samples from Saccharum species and interspecific hybrids from the Miami World Collection of sugarcane and related grasses were tested for the presence of SStrV and SWSV by PCR. No variety was found infected by SWSV but SStrV was detected in 19 varieties. PCR data were confirmed by sequencing amplified fragments (248 bp). These fragments shared 93%–100% nucleotide identity with SStrV sequences from the GenBank database. SStrV isolates were distributed in six phylogenetic groups, including the four strains of the virus. Most varieties infected by SStrV originated from Asia, thus confirming a previous hypothesis stating that this virus originated from this continent. Absence of SStrV in commercial sugarcane in Florida also suggested that this virus has not been spread in this location, while infected plants have been present for several decades.  相似文献   

Validity of goniometric joint measurements in cats   总被引：2，自引：0，他引：2  

Jaeger GH  Marcellin-Little DJ  Depuy V  Lascelles BD 《American journal of veterinary research》2007,68(8):822-826

OBJECTIVE: To compare and validate goniometric joint measurements obtained from nonsedated and sedated cats with measurements from radiographic evaluation. ANIMALS: 20 adult cats with no evidence of joint disease. PROCEDURES: Measurements of flexion and extension of the carpus, elbow, shoulder, tarsus, stifle, and hip joints and of carpal and tarsal joints during varus and valgus angulation were made by a single investigator before and after sedation of cats. Measurements were made by use of a goniometer with a masked dial. Joint angle measurements were compared between nonsedated and sedated cats and also with measurements from radiographs made while cats were sedated. Each series of measurements was repeated 4 times. To evaluate repeatability, Cronbach alpha values were calculated for repeated measure results of goniometric joint measurements of nonsedated and sedated cats. An intraclass correlation was calculated to determine reliability among the 3 measurement types (ie, measurements from nonsedated and sedated cats and on radiographic evaluation). RESULTS: Joint measurements did not differ significantly by measurement type, when comparing radiographic measurements with goniometric measurements in sedated and nonsedated cats. Cronbach alpha values were > 0.99 for goniometric joint measurements within individual nonsedated and sedated cats and also for comparison of mean measurements obtained from sedated cats versus nonsedated cats versus radiographs. An intraclass correlation of 0.999 revealed high reliability among measurement types. CONCLUSIONS AND CLINICAL RELEVANCE: Results indicated that goniometric joint measurements in nonsedated and sedated cats are repeatable and valid.  相似文献   

Distribution and metabolism of abamectin in larval stages of Spodoptera littoralis boisduval and Heliothis armigera hübner (Lepidoptera: Noctuidae)     

Paul T. Christie  Denis J. Wright 《Pest management science》1991,31(2):249-261

Comparisons were made between the amounts of abamectin and total radioactivity recovered from Spodoptera littoralis and Heliothis armigera larvae after topical application of [³H]abamectin. Penetration (as shown by wash-off experiments) did not differ significantly between the Spodoptera instars. Significantly more abamectin was recovered from ventral nerve cord samples of larvae showing symptoms of poisoning than from larvae not showing these symptoms. Fifth-instar S. littoralis larvae had a significantly lower proportion of radioactivity as abamectin in the ventral nerve cord than in sixth-instar S. littoralis or fifth- and sixth-instar H. armigera. The proportion of radioactivity present as abamectin (but not total radioactivity) was significantly increased when the fifth-instar S. littoralis larvae were pre-treated with piperonyl butoxide (PB) suggesting that the relative insensitivity of fifth-instar S. littoralis larvae to abamectin is due at least in part to greater metabolism, particularly by microsomal oxidases. Fat body samples consistently had a greater proportion of radioactivity as abamectin than the nerve cord and the former may act as storage sites for abamectin.  相似文献   

Introduction to veterinary physical rehabilitation.     

David Levine  Darryl L Millis  Denis J Marcellin-Little 《Veterinary Clinics of North America: Small Animal Practice》2005,35(6):1247-54, vii

Physical therapy is a profession with an established scientific basis in human beings and companion animals. It has a large number of clinical applications in the restoration, maintenance, and promotion of optimal physical function. In providing physical therapy, the goal is to restore, maintain, and promote optimal function, optimal fitness, wellness, and quality of life as they relate to movement disorders and health. A major emphasis is to prevent or minimize the onset, clinical signs, and progression of impairments, functional limitations, and disabilities that may result from diseases, disorders, conditions, and injuries.  相似文献   

Rehabilitation and conditioning of sporting dogs.     

Denis J Marcellin-Little  David Levine  Robert Taylor 《Veterinary Clinics of North America: Small Animal Practice》2005,35(6):1427-39, ix

Owners and trainers exercise sporting dogs to increase their fitness and optimize their conditioning and performance. Training is designed to in-crease strength, endurance, and agility and is sport-specific. Sporting dogs are susceptible to specific musculoskeletal injuries. The rehabilitation of sporting dogs after these injuries follows specific principles during the acute, subacute, and reconditioning periods.  相似文献   

Contact activity of synthetic pyrethroids against adult grass grub,Costelytra zealandica (white)     

Ronald F. Henzell  Denis R. Lauren 《Pest management science》1978,9(6):582-586

The field life for contact activity of cypermethrin and permethrin, applied to pasture, was similar for all of five application rates between 0.25 and 1.5 kg ha^?1. Laboratory bioassays with grass grub beetles, Costelytra zealandica (White), showed that the insecticides lost their activity after about 4–9 days on pasture. Microencapsulation significantly increased the active field life of permethrin.  相似文献   

Characteristics of reproduction in the zebu, studied at the Dahra Center for Zootechnical Research     

J Denis  A I Thiongane 《Revue d'élevage et de médecine vétérinaire des pays tropicaux》1973,26(4):49a-60a

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