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1.
Three oat (Avena saliva L.) populations (i.e., lines of descent), high grain yield (HG), high protein content (HP), and high protein yield per se (HGP), each developed by three cycles of S, recurrent selection, were evaluated for the effect of selection for groat-protein yield upon other agronomic traits. Selections making up the HG line of descent had high protein yield primarily due to high grain yield, and those selected for HP had high protein yield due to both high protein content and high grain yield. Selection in HGP was on the basis of protein yield per se. Selection caused increases in bundle weight, harvest index, vegetative growth rate, and seed number in all lines of descent. Heading date, plant height, and seed weight were unaffected, whereas groat percentage and test weight were decreased in HP and HGP. Heritabilities were high for heading date, plant height, test weight, and seed weight, moderate for harvest index and bundle weight, and low for groat percentage. Genetic variability generally declined from CO to C3 for all traits. Groat-protein yield and amount of protein per groat increased in all lines of descent. In HG, the increase in groat weight was due primarily to increases in the nonprotein fraction, with groat-protein content actually decreasing. In HGP, groat weight increased due to increases in protein and nonprotein fractions, and groat protein content remained constant. In HP, groat weight and amount of nonprotein per groat decreased, thus increasing groat-protein content. Three cycles of recurrent selection resulted in oat lines with groat-protein yields significantly higher than the highest lines from CO. Utilization of index selection may be desirable to obtain populations of greater breeding value.  相似文献   
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Changes in blood gases, pH, and plasma electrolyte concentrations in response to intravenously infused sodium bicarbonate (NaHCO3) and sodium acetate (NaCH3CO2) solutions (1.34 mEq/mL) in 5 light breed mares were investigated. Jugular venous blood samples were collected before and after completion of the infusions in 20-minute intervals for 200 minutes. Infusion of sodium bicarbonate and sodium acetate caused significant (P < .00l) increases in blood pH and bicarbonate ion concentration that persisted throughout the collection period. The elevation in blood pH and bicarbonate ion concentrations was greater (P < .01) for sodium bicarbonate than for sodium acetate immediately after the completion of the infusions but was not different (P > .05) thereafter. There were significant reductions (P < .01) in plasma-ionized calcium and potassium after infusion of both sodium bicarbonate and sodium acetate. This study found that significant metabolic alkalosis in horses and corresponding shifts in electrolyte concentrations can be induced by intravenous infusion of solutions of either sodium bicarbonate or sodium acetate, and they persist for at least 3 hours. These data show that the short-term elevation in pH and bicarbonate ion concentration is momentarily higher after infusion of sodium bicarbonate. This is likely due to the direct infusion of bicarbonate ions in the sodium bicarbonate treatment, such that further metabolism is not required to be effective. However, the longer-term alkalosis did not differ between isomolar solutions of sodium bicarbonate and sodium acetate.  相似文献   
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Overweight and obesity are multifactorial diseases caused by an imbalance in energy metabolism. An underlying genetic predisposition is often a factor in these conditions. In the cat breeding family of the Institute of Animal Nutrition at the Vetsuisse Faculty, University of Zurich, a segregating overweight phenotype with a genetic contribution was observed. From this breeding family, 26 kittens were followed from birth up to 8 months of age. During this time, food intake was measured using an automatic feeding station, and energy expenditure was investigated using indirect calorimetry at the ages of 4 and 6 months. Dual‐energy X‐ray absorptiometry (DEXA) was performed and blood glucose, leptin and insulin were measured at the ages of 4, 6 and 8 months. The kittens were also weighed daily for the first 2 weeks of life, every second day until weaning and once per week until 8 months of age. The body condition score (BCS) was evaluated monthly between 2 and 8 months of age. The main finding of this study is that a predisposition to overweight is connected to a higher food intake early in life, with no significant alterations in energy expenditure. The leptin blood levels were related to body fat percentage, and insulin sensitivity did not seem to be affected.  相似文献   
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The serological cross reactions between Mycoplasma conjunctivae, the etiological agent of infectious keratoconjunctivitis (IKC), and the antigenetically and phylogenetically closely related Mycoplasma ovipneumoniae, which is often found in sheep, were analysed. Cross reacting antigens were identified using sera from sheep with IKC and from sheep of herds known to be free of IKC, as well as rabbit hyperimmune serum specific to the two Mycoplasma species. Cross reactions were predominantly due to the strongly antigenic proteins of 42 kDa and 83 kDa. Serospecific antigens of M. conjunctivae could be separated from cross-reacting antigens by the extraction of Tween 20-soluble membrane proteins. The Tween 20-extracted proteins of the M. conjunctivae strain HRC/581T were used for the development of an indirect ELISA test. This ELISA test was shown to be a useful serological method for the diagnosis of M. conjunctivae infections and to identify infected sheep herds.  相似文献   
6.
In the Norddeich orphanage seals about four to five months of age were exposed intranasally and by contact to a phocine herpesvirus which originated from a young seal in this station. Virus excretion by the nasal route was observed in two animals on day 2 and 15 post inoculation, respectively. Production of neutralizing antibodies was detected one week after exposure. The antibody titre range was approximately tenfold higher in the inoculated seals than in animals of the contact group. The seals did not become seriously sick.  相似文献   
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We assessed and compared host cell specificity of the haemolytic and cytotoxic activity of the RTX toxins from Actinobacillus equuli, an equine pathogen, and Actinobacillus suis, which is pathogenic for pigs. The two bacterial species are closely related, phenotypically as well as phylogenetically, sharing the same 16S rRNA gene sequence. Both species contain specific protein toxins from the family of pore-forming RTX toxins, however, the two species differ in their RTX toxin profiles. Haemolytic A. equuli contains the operon for the Aqx toxin, whereas A. suis harbours genes for ApxI and ApxII. We tested the toxic activity of the corresponding proteins on erythrocytes as well as on lymphocytes isolated from horse and pig blood. The strength of the haemolytic activity for each of the toxins was independent of the origin of erythrocytes. When testing cytotoxic activity, the Aqx protein showed a higher toxic effect for horse lymphocytes than for porcine lymphocytes. On the other hand, ApxI and ApxII showed a strong cytotoxic effect on porcine lymphocytes and a reduced toxicity for horse lymphocytes; the toxicity of ApxII was generally much lower than ApxI. Our results indicate a host species specificity of the toxic activity of RTX toxins Aqx of A. equuli and ApxI and ApxII of A. suis.  相似文献   
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