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The complete genomic sequences of several Pseudomonas spp. that inhabit the rhizosphere are now available, providing a new opportunity to advance knowledge of plant growth-promoting rhizobacteria (PGPR) through genomics. Among these is the biological control bacterium Pseudomonas fluorescens Pf-5. Nearly 6% of the 7.07 Mb genome of Pf-5 is devoted to the biosynthesis of secondary metabolites, including antibiotics toxic to soilborne fungi and Oomycetes that infect plant roots, and two siderophores involved in iron acquisition. Three orphan gene clusters, for which the encoded natural product was unknown, also were identified in the genome of Pf-5. The product synthesized from one of the orphan gene clusters was identified recently using a new ‘genomisotopic approach’, which employs a combination of genomic sequence analysis and isotope guided fractionation. Application of the genomisotopic approach to one orphan gene cluster in Pf-5 resulted in the discovery of orfamide A, founder of a new group of bioactive cyclic lipopeptides with a putative role in biological control of plant disease.  相似文献   
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Angular deformities of 25 or 37 degrees valgus were created by transverse wedge osteotomies in the diaphysis of the radius in 3-month-old Cocker Spaniels. Spontaneous correction was judged from radiographs until skeletal maturity. The magnitude of correction was estimated, using the opposite normal leg as a control. Asymmetrical physeal growth corrected the induced angulation between the proximal and distal radial joint plane surfaces (joint plane angle). The average correction of the joint plane angle was 80% in the 25 degree group, and 72% in the 37 degree group. Bone remodeling of the radial shaft corrected the induced diaphyseal angulation by 50% in both groups during the study.  相似文献   
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The effect of spraying winter rape and winter wheat with deltamethrin on the numbers of epigeal Carabidae, Staphylinidae and Linyphiidae caught in pitfall traps was studied in Northern Germany in 1980 and 1981. Two experiments were performed with each crop, deltamethrin being sprayed at 7.5 g a.i. ha?1 in 300 litres of water in springtime on rape, and in midsummer on wheat. Sprayed plots were rectangles of 2 and 3 ha; unsprayed plots were squares of 0.5 ha each (two replicates). Ten pitfall traps were placed per replicate at least 3 weeks before spraying and were left for at least 3 weeks after spraying, the traps being emptied weekly. Beneficial Carabid species which have been shown already to have an economic significance, were rare during this study, but four other species (three of them were autumn breeders) were not affected by deltamethrin. At the spraying dates the autumn breeders were hidden in the soil as pupae. Three Staphylinid species, studied in only one experiment each, appeared to be strongly reduced in numbers by deltamethrin. The Linyphiid spiders were affected most heavily by deltamethrin sprayed in spring or in midsummer; the initial mortality of more than 92% lasted longer than 4 weeks, and an adverse effect was still significant in winter wheat 6 weeks after spraying. Overall, deltamethrin appears to have a broad spectrum effect concerning the epigeal predatory arthropods.  相似文献   
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A recirculation unit for salmon smolt production is described. The preliminary results for growth rate and viability of the fry were satisfactory.  相似文献   
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Mixed tree cropping systems have been proposed for sustainable nutrient management in the humid tropics. Yet, the nutrient interactions between intercropped trees have not been addressed sufficiently. In the present study we compare the temporal and spatial patterns of the uptake of applied 15N by four different tree crops in a mixed tree cropping system on a Xanthic Ferralsol in central Amazônia, Brazil, during one year. Most of the N uptake occurred during the first two weeks. Very little N was recovered by peach palm (Bactris gasipaes), more by cupuassu (Theobroma grandiflorum) and annatto (Bixa orellana) and most by Brazil nut (Bertholletia excelsa). Due to tree pruning the total accumulation of applied 15N in the above-ground biomass of annatto decreased throughout the year. It remained constant in cupuassu and peach palm and increased in Brazil nut. Brazil nut showed an extensive root activity and took up more fertilizer N applied to neighboring trees than from the one applied under its own canopy in contrast to the other three tree crops. Therefore, trees with wide-spread root systems may not need to receive N fertilizer directly but can take up N applied to other trees in the mixed cropping system. This means that such trees may effectively decrease N leaching when intercropped with trees that have dormant periods or places with low N uptake, but also exert considerable resource competition.This revised version was published online in November 2005 with corrections to the Cover Date.  相似文献   
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Studies on carbohydrate digestion in rainbow trout   总被引:5,自引:0,他引:5  
Trout are poor starch digesters. The reason for the different digestibilities of carbohydrates were investigated and discussed. The inclusion of soluble starch products in the diet increases the volume of intestinal juices. Crude starch in the diet reduces amylase activity, amylase is adsorbed to crude starch so that starch hydrolysis is inhibited. Crude starch accelerates the passage through the intestine, thus reducing the time available for absorption.  相似文献   
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Rabbit-fish, Siganus oramin, reared in a closed seawater system on an artificial diet, were brought to maturity and spawned artifically after human chorionic gonadotropin (HCG) injections. Eggs were incubated under different salinity regimes. Hatching success between 20.9 and 32.2‰ S was ≥95%.  相似文献   
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