Culture of bovine bone marrow progenitor cells in vitro.     

V Van Merris  M Lenjou  D Hoeben  G Nijs  D Van Bockstaele  C Burvenich 《The Veterinary quarterly》2001,23(4):170-175

In vitro methylcellulose cultures of bovine bone marrow progenitor cells were developed. An existing technique described for bovine species was compared to a method for human tissue and further adapted during subsequent experiments. Bovine bone marrow samples were collected at the slaughterhouse, and mononuclear cells were separated by gradient centrifugation (1.077 g/ml specific density and 400 g). The use of 3% bovine leucocyte-conditioned medium, produced by stimulation of blood lymphocytes with 4 microg/ml concanavalin A and harvested on day 4 of culture, gave better results than the use of supernatant of the human bladder carcinoma 5637, which is widely used in human bone marrow cultures. However, bovine leucocyte-conditioned medium was not added to erythroid cultures because inhibitory effects were observed. Erythroid colonies were stimulated with erythropoietin, and hemin was added to enable microscopic identification. Reduced oxygen tension was necessary to induce growth of erythroid colonies. This was not necessary for myeloid cultures. In conclusion, the results of this study show that the growth of myeloid and erythroid colonies in methylcellulose-based medium requires different culture conditions, which are different from the culture conditions for human cells.  相似文献   

Familial stomatocytosis--hypertrophic gastritis (FSHG), a newly recognised disease in the dog (Drentse patrijshond)     

R J Slappendel  I van der Gaag  J J van Nes  T S van den Ingh  R P Happé 《The Veterinary quarterly》1991,13(1):30-40

A newly recognised disease, which we have given the provisional name of familial stomatocytosis-hypertrophic gastritis (FSHG), is described in two families of dogs of the Drentse partrijshond breed. The affected dogs consisted of 3 females and 5 males, 3 to 19 (mean 9.5) months of age at admission. The main clinical problems were diarrhoea, icterus, and ataxia and paresis of the pelvic limbs. Laboratory evaluation revealed abnormal red cell shape (stomatocytosis), increased osmotic fragility, haemolytic anaemia, and increased liver enzymes and serum bilirubin. Gastroscopic and histopathologic examination of the gastric mucosa revealed hypertrophic gastritis resembling Ménétrier's disease in man. Histologic findings in the liver were suggestive of progressive liver disease. Cysts were found in the kidneys of the five oldest patients. Electroneurography in 2 dogs revealed polyneuropathy. In the parents of 2 patients (sister and brother), there were no clinical or laboratory abnormalities. An autosomal recessive hereditary defect of lipid metabolism is suspected.  相似文献   

Mystery swine disease in The Netherlands: the isolation of Lelystad virus.   总被引：115，自引：0，他引：115  

G Wensvoort  C Terpstra  J M Pol  E A ter Laak  M Bloemraad  E P de Kluyver  C Kragten  L van Buiten  A den Besten  F Wagenaar 《The Veterinary quarterly》1991,13(3):121-130

In early 1991, the Dutch pig-industry was struck by the so-called mystery swine disease. Large-scale laboratory investigations were undertaken to search for the etiological agent. We focused on isolating viruses and mycoplasmas, and we tested paired sera of affected sows for antibodies against ten known pig viruses. The mycoplasmas M. hyosynoviae, M. hyopneumoniae, and Acholeplasma laidlawii, and the viruses encephalomyocarditis virus and porcine enterovirus types 2 and 7 were isolated from individual pigs. An unknown agent, however, was isolated from 16 of 20 piglets and from 41 of 63 sows. This agent was characterised as a virus and designated Lelystad virus. No relationship between this virus and other viruses has yet been established. Of 165 sows reportedly afflicted by the disease, 123 (75 per cent) seroconverted to Lelystad virus, whereas less than 10 per cent seroconverted to any of the other virus isolates or to the known viral pathogens. Antibodies directed against Lelystad virus were also found in pigs with mystery swine disease in England, Germany, and in the United States. We conclude that infection with Lelystad virus is the likely cause of mystery swine disease.  相似文献   

Quantitative Aspects of Thyroid Scintigraphy With Pertechnetate (^99mTcO₄) in Cats     

Ariana M.P. Nap  Yvonne W.E.A. Pollak  Walter E. van den  Brom Ad Rijnberk DVM  PhD 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》1994,8(4):302-303

Thyroidal ^99mTcO₄(pertechnetate) uptake percentages were determined in unanesthetized euthyroid (n = 13) and hyperthyroid (n = 18) cats. Maximal uptakes were observed 60 minutes after IV injection of the radionuclide and ranged from 0.3 to 3.9% of the dose in euthyroid cats (median 2.23%) and from 5.2% to 23.9% of the dose in hyperthyroid cats (median 14.8%) ( P < .05). There were no overlaps in pertechnetate uptake percentages during any of the intervals evaluated. It is concluded that the optimal time for visualization of the thyroid by ^99mTcO₄-scanning is 60 minutes after IV injection of the radionuclide. Calculation of the percentage uptake is of additional diagnostic value.  相似文献   

Landscape-ecological mapping of the Netherlands   总被引：1，自引：0，他引：1  

Kees J. Canters  Cees P. den Herder  Aart A. de Veer  Paul W. M. Veelenturf  Rein W. de Waal 《Landscape Ecology》1991,5(3):145-162

The Landscape-ecological Mapping of the Netherlands project (LMN project) started in 1983 with the aim of establishing a landscape-ecological database for use in developing and evaluating national land-use plans. The project, working with grid cells of 1 km², has four working objectives: a) development of mapping potential for basic landscape-ecological data, b) assessment of susceptibility to interventions, c) evaluation of significance for nature conservation and d) production of vulnerability maps, as a combination of susceptibility and significance. In addition to information on soil, groundwater, ecotopes, flora and fauna, the database also incorporates information on physiographical features and entire landscapes. The resulting database is a geographic information system (GIS). This article describes the second phase of the project (1985–1989), covering the Randstad area, and focusses on the methods and the applications potential of the database.  相似文献   

Covalently protein-bound bilirubin conjugates in cholestatic disease of dogs     

J Rothuizen  T van den Ingh 《American journal of veterinary research》1988,49(5):702-704

We investigated the occurrence of covalently protein-bound bilirubins in the plasma of dogs with hyperbilirubinemia attributable to hepatobiliary diseases or Coombs test-positive hemolysis. The bilirubins in plasma were measured with the conventional Van den Bergh reaction, by treatment with diazotized p-iodoaniline, and by high-performance liquid chromatography of bilirubin and its methylesters after alkaline methanolysis. All but one dog had covalently protein-bound bilirubin conjugates. The concentration and the fraction of total bilirubins varied in all diseases investigated, but they tended to be low in primary hemolysis. The "biliprotein" complex accounted for 2 to 94% of total plasma bilirubins. Because biliprotein usually is not cleared by the liver, but has a half-life comparable with that of albumin, it prevents the evaluation of the actual state of the underlying disease. Measurement of the total bilirubin concentration exclusively with the Van den Bergh reaction, therefore, is clinically useless. Other methods should be introduced for routine bilirubin assays, permitting the measurement of noncovalently bound pigment as a meaningful estimate of the course of the disease.  相似文献   

Optimal cultivar mixtures in focal plant diseases     

F. van den Bosch 《European journal of plant pathology / European Foundation for Plant Pathology》1993,99(Z3):41-50

This paper deals with a study on the effects of mixtures of partially resistant cultivars in focus forming plant diseases. The focus expansion velocity in a mixture of two partially resistant cultivars depends on four parameters, the focus expansion velocity in a crop of cultivar 1 only and in a crop of cultivar 2 only, the net-reproduction in a crop of cultivar 1 and of cultivar 2, respectively. An explicit equation is developed for the damage of a cultivar mixture where the crop is infected by two physiological races of the pathogen. It is shown that cultivar mixtures exist where damage is smaller than in crops of either of the two cultivars only. A simple explicit formula is given on the basis of which it can be decided whether an optimal mixture exists for a given pathosystem.  相似文献   

Effects of air temperature, air movement and artificial rain on the heat production of brushtail possums (Trichosurus vulpecula): an exploratory study     

van den Oord QG  van Wijk EJ  Lugton IW  Morris RS  Holmes CW 《New Zealand veterinary journal》1995,43(7):328-332

Two groups of six mature brushtail possums (Trichosurus vulpeculu) were housed in two respiration chambers, and their heat production, whole body conductance and lower critical temperatures were measured under a variety of simulated weather patterns. The possums were subjected to ambient temperatures of 30, 20 and 3 degrees C. At 20 and 3 degrees C, the animals were exposed to near still air and light winds (wind speed 0.8 and 6.7 km/h), both with, and without, simulated rain every 8 hours. The lower critical temperature in near still air lies between 7 and 10 degrees C. This temperature increases by about 2, 6 and 8 degrees C respectively for a wind velocity of 6.7 km/h, simulated rain and a combination of the two factors. Weather in New Zealand, especially in the cooler part of the year, will often produce conditions below the lower critical temperature of the thermoneutral zone of possums. This will necessitate significant increases in metabolic rate and hence food consumption or mobilisation of body fat reserves, which if not sustainable will result in the death of possums. Field studies have shown that this is often the case in the wild. It is proposed that this stress may be sufficient to decrease the resistance (especially cell-mediated immunity) of some possums and allow acceleration of the disease process in those infected with Mycobacterium bovis.  相似文献   

Comparison of aloe vera and omeprazole in the treatment of equine gastric ulcer syndrome          下载免费PDF全文

J. Bush  R. van den Boom  S. Franklin 《Equine veterinary journal》2018,50(1):34-40

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An improved vitrification protocol for equine immature oocytes,resulting in a first live foal          下载免费PDF全文

N. Ortiz‐Escribano  O. Bogado Pascottini  H. Woelders  L. Vandenberghe  C. De Schauwer  J. Govaere  E. Van den Abbeel  T. Vullers  C. Ververs  K. Roels  M. Van De Velde  A. Van Soom  K. Smits 《Equine veterinary journal》2018,50(3):391-397

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