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The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy. 相似文献
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目的探讨SLeX和CK19蛋白在甲状腺乳头状癌中的表达及其与癌组织浸润转移的关系。方法应用免疫组化染色EliVisionTMplus两步法检测127例甲状腺乳头状癌组织和30例癌旁甲状腺组织中SLeX和CK19蛋白的表达状况。结果甲状腺乳头状癌组织SLeX和CK19总体阳性表达率分别为70.1%和96.1%,显著高于癌旁甲状腺组织(P0.05);存在浸润和伴有淋巴结转移者,SLeX和CK19阳性表达率显著高于无浸润和无淋巴结转移者(P0.05);SLeX与CK19表达具有一定正相关性(r=0.30)。结论 SLeX和CK19在甲状腺乳头状癌组织中多数呈强阳性表达,两者与癌组织浸润转移密切相关。 相似文献
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AIM: To observe the effect of microRNA-19a (miR-19a) on the lipid catabolism of hepatocyte LO2, and to explore the potential mechanism. METHODS: miR-19a was over-expressed or silenced by transfection of miR-19a mimics or miR-19a inhibitor into LO2 cells, then the mRNA level of miR-19a was detected by real-time PCR. The potential target of miR-19a was found by the method of bioinformatics through internet website. The effect of miR-19a on the 3' UTR of peroxisome proliferator-activated receptor α (PPARα) was measured by dual luciferase reporter assay, and the protein level of PPARα and its 2 major downstream rate-limiting enzymes involved in lipid catabolism, acyl-coenzyme a dehydrogenase (ACADM) and carnitine palmitoyltransferase 1A (CPT1A), were detected by Western blotting. Meanwhile, the effect of miR-19a on the generation of ketone body was measured by beta-hydroxybutyric acid (β-OHB) detection assay. RESULTS: The mRNA level of miR-19a was dramatically elevated by the transfection of miR-19a mimics, and sharply decreased by the transfection of miR-19a inhibitor (P<0.05). PPARα was found as a potential target of miR-19a, and dual luciferase reporter assay and Western blotting confirmed the regulatory effect of miR-19a on the expression of PPARα, with the protein level changes of ACADM and CPT1A. miR-19a mimics down-regulated, while miR-19a inhibitor up-regulated the concentration of β-OHB in LO2 cells (P<0.05). CONCLUSION: miR-19a regulates the lipid catabolism of hepatocytes by targeting the PPARα and its 2 downstream rate-limiting enzymes. 相似文献
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基于2020年3月-2020年10月猪肉价格及新冠肺炎疫情指数,借助VAR模型,定量研究新冠肺炎疫情对于猪肉价格的影响。研究结果表明:新冠肺炎疫情对猪肉价格存在影响,促使猪肉价格上涨,在此基础上文章提出相应措施。 相似文献
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Toshitaka KANEI Munetaka IWATA Hiroaki KAMISHINA Takuya MIZUNO Sadatoshi MAEDA 《The Journal of veterinary medical science / the Japanese Society of Veterinary Science》2022,84(1):25
C-C chemokine receptor 7 (CCR7) contributes to cell homing to lymph nodes (LNs). Recent studies reported that CCR7 is also expressed in tumor cells, which correlates with LN metastasis in various cancers. However, the expression of CCR7 in tumor cells is unknown in dogs due to the lack of appropriate antibodies. In the present study, a fusion protein of C-C chemokine ligand 19 (CCL19) was employed as an alternative method to CCR7 antibodies. The fusion CCL19 protein specifically detected CCR7 expressed in canine lymphoma cell lines, which showed active chemotaxis to both canine and mouse ligands. The present study will help further research on the involvement of canine CCR7 in LN metastasis. 相似文献
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