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本文测定了温度对香菇胞外纤维素酶和半纤维素酶活性的影响。木屑基质培养90天的香菇胞外游纸纤维素酶（C_1酶）、羧甲基纤维素酶（C_X酶）和半纤维素酶（H_C酶）的最适作用温度分别是55℃、55～60℃和50℃。在50～55℃条件下保温1小时后,C_1酶和H_C酶活性大幅度下降,而C_x酶活性下降较为缓慢,具有较高的热稳定性。本文提出相对活力系数（Relative activity coefficient,RAC）作为表示香菇菌丝生长温度与酶最适作用温度之间关系的一个参数。 相似文献
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研究了半纤维素酶Ｅ－Ａｎ－７６作为漂白助剂对桦木硫酸盐浆及麦草烧碱蒽醌浆ＣＥＨ三段漂的影响。结果表明：桦木ＫＰ浆用Ｅ－Ａｎ－７６预处理后再经ＣＥＨ三段漂，其白度明显高于相同用氯量下未用酶预处理的漂白浆。另一方面在保持白度不变的情况下酶处理浆的漂白可节省近５０％的用氯量。麦草Ｓｏｄａ－ＡＱ浆经Ｅ－Ａｎ－７６预处理后再经ＣＥＨ三段漂与酶未处理浆的ＣＥＨ三段漂比较亦可明显提高白度，但得率下降严重。通过对桦木ＫＰ浆酶解液中还原糖以及单糖组成分析，发现浆中再沉积或回吸的木聚糖在Ｅ－Ａｎ－７６助剂漂白中起着重要作用。 相似文献
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袋栽香菇生长期间不同料层中木质纤维素的降解及有关酶活性的变化 总被引：4，自引：2，他引：2
本文研究了香菇生长过程菌袋中不同料层木质纤维素的降解及有关酶活性的变化。结果表明,在栽培结束时,菌袋中不同料层木质纤维素含量的差异不大;在整个栽培过程中,菌袋中不同料层羧甲基纤维素酶,半纤维素酶和漆酶活性的变化大致相同。就菌袋中不同料层木质纤维素的降解和利用而言,菌袋的中层和下层与上层相似。 相似文献
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Annemieke van der Wal Johannes A. van Veen Richard C. Summerbell 《Soil biology & biochemistry》2006,38(9):2890-2902
In an earlier study we reported the apparent stabilization of a low fungal biomass in ex-arable lands during the first decades after abandonment. It was hypothesized that the lack of increase in fungal biomass was due to constraints on development of fungi with persistent hyphae such as lignocellulolytic basidiomycetes and ericoid mycorrhizal fungi. With respect to the former group, the slow increase of the pool of lignocellulose-rich organic matter was expected to be the major constraint for their development. To study this, we enriched soil samples of one arable land, of two recently abandoned arable lands, of one older abandoned arable land and of heathland with carbon substrates that differed in composition (glucose, cellulose and sawdust). In addition, we combined the effect of carbon addition on fungal biomass development in arable and recently abandoned lands with inoculation of 1% of soil from the older abandoned site and the heathland. All treatments induced a fast increase and a subsequent rapid decline in fungal biomass in the arable and ex-arable fields. Denaturing Gradient Gel Electrophoresis (DGGE) band patterns and enzyme activities did show differences between the carbon treatments but not between the recent and older abandoned field sites, indicating a similarly responding fungal community even after three decades of land abandonment and irrespective of soil inoculation. Identification of fungi by sequencing and culturing confirmed that decomposition processes were mostly dominated by opportunistic fungi in arable and ex-arable fields. In the heathland, only a very slow increase of microbial activity was observed after addition of carbon and sequencing of DGGE bands showed that ericoid mycorrhiza (ERM) fungi were responsible for carbon decomposition. We conclude that an increase of enduringly present fungal hyphae in ex-arable land may only be possible when a separate litter layer develops and/or when suitable host plants for ERM fungi become established. 相似文献
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秸秆腐熟快速启动条件的研究 《勤云标准版测试》2005,(5)
以加速秸秆降解速度为出发点,研究了速效碳源、速效氮源和接种量对M1、M15、M19混合菌产纤维素酶和半纤维素酶能力的影响。结果表明,蔗糖和尿素是秸秆降解的首选速效碳源和氮源,通过正交实验筛选出蔗糖、尿素和接种量的最适配比为2.0%、1.4%和10%。 相似文献
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用4种饲料在冬季饲养矮小型蛋鸡,A组为对照组,使用不加油或和美酵素的基础日粮;B组日粮是基础日粮添加0.5%油脂和500g/T和美酵素;C组日粮是基础日粮添加油脂;D组日粮是在基础日粮基础上加500g/T和美酵素,实验期11周。结果表明,使用基础日粮加和美酵素的D组产蛋率最高穴77.7%雪,和其它3个组差异显著(P<0.05雪,实验期料蛋比2.21押1熏每只鸡毛利3.52元,比对照组和加油组分别高0.45元和0.79元。结果也表明熏在代谢能较高的饲料中添加和美酵素,会使矮小型鸡的体重显著增加,并不能显著提高产蛋水平,因此和油脂同时使用效果不好。 相似文献
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