Auxins increase the occurrence of leaf-colour variants in Caladium regenerated from leaf explants     

E. U. Ahmed  T. Hayashi  S. Yazawa 《Scientia Horticulturae》2004,100(1-4):153-159

Leaf explants of Caladium ‘Pink Cloud’ were cultured in vitro on MS medium containing various auxins (NAA, IBA, IAA, 2,4,5-T and 2,4-D) in combination with cytokinin (BA). NAA gave the most vigorous in vitro propagation of this plant, and only 15% of the plants were leaf-colour variants on the medium containing 0.5 μmol NAA. Leaf colour variation was observed in all plants regenerated on the medium containing 2,4-D at 0.5–4.5 μmol. In hormone-free medium, only a few leaf-colour variants (6%) occurred, but the rate of plant regeneration was very low. Application of 0.5 μmol NAA together with 4.5 μmol BA seemed to be the most appropriate for in vitro propagation of Caladium ‘Pink Cloud’ with only a few leaf-colour variants.  相似文献   

Assessment of clonal fidelity of micropropagated gerbera plants by ISSR markers     

R. Bhatia  K.P. Singh  T. Jhang  T.R. Sharma 《Scientia Horticulturae》2009

True-to-type clonal fidelity is one of the most important pre-requisites in micropropagation of crop species. Genetic fidelity of in vitro raised 45 plants of gerbera (Gerbera jamesonii Bolus) derived from three different explants, viz., capitulum, leaf and shoot tips, was assessed by 32 ISSR markers, for their genetic stability. Out of 32 ISSR markers, 15 markers produced clear, distinct and scorable bands with an average of 5.47 bands per marker. The markers designed from AG motif amplified more number of bands. The markers anchored at 3′ ends produced high number of consistent bands than unanchored markers. Fifteen ISSR markers generated a total of 3773 bands, out of which 3770 were monomorphic among all the clones. The Jaccard's similarity coefficient revealed that out of 45 clones derived from different explants, 44 were grouped into a single large cluster alongwith the mother plant with a similarity coefficient value of 1.00, whereas one clone (C38) remained ungrouped. The clones derived from capitulum and shoot tip explants did not show any genetic variation, whereas, one of the leaf-derived clones exhibited some degree of variation.  相似文献   

Enhanced micropropagation of Dendrobium huoshanense C.Z. Tang et S.J. Cheng through protocorm-like bodies: The effects of cytokinins,carbohydrate sources and cold pretreatment     

Jian-Ping Luo  Christoph Wawrosch  Brigitte Kopp 《Scientia Horticulturae》2009

The effects of cytokinins, carbohydrate sources and cold pretreatment on the conversion of protocorm-like bodies (PLBs) to shoots were investigated for the enhancement of micropropagation of Dendrobium huoshanense C.Z. Tang et S.J. Cheng, an endangered medicinal plant in China. The effects of cytokinins and carbohydrate sources on the conversion of PLBs to shoots depended on their types and concentrations. The best results in terms of shoot development from PLBs occurred on 1/2 MS medium supplemented with 20 μM kinetin and 10 g l⁻¹ maltose. Cold pretreatment at 10 °C for 1–2 weeks significantly enhanced the conversion of PLBs to shoots, and over 1300 shoots were obtained from one gram of PLBs after 3 months of culture. The developed shoots were rooted on growth regulator-free MS medium supplemented with 8 g/l banana paste to give complete plantlets, which were successfully acclimatized with a survival rate of approximately 65%. The results indicate that a suitable cold pretreatment (10 °C for 1 week) followed by the use of 20 μM kinetin and 10 g/l maltose in 1/2 MS medium would produce a large number of shoots from PLBs for plantlet regeneration of D. huoshanense.  相似文献   

基于均匀设计法优化毛毡杜鹃高效快繁体系     

顾地周  高捍东  曹逊  马秋月  顾美影  朱俊义 《林业科技》2009,34(2):57-59

以毛毡杜鹃嫩茎段为外植体,应用均匀设计法筛选最适合的培养基。试验结果表明,适合嫩茎段腋芽萌发生长及生根的最佳培养基为：MS（改良）＋IAA 0．10mg/L＋IBA 0．30mg／L＋NAA 0．12mg／L＋GA 31．80mg／L,再生率达93％以上。以再生植株的茎节为材料进行快繁的结果表明,在25天的一个培养周期内,茎段的增殖倍数平均达50以上。  相似文献   

Influencing in vitro clonal propagation of Chonemorpha fragrans(moon) Alston by culture media strength,plant growth regulators,carbon source and photo periodic incubation     

Tasiu Isah  Shahid Umar 《林业研究》2020,31(1):27-43

Chonemorpha fragrans is an endangered medicinal woody climber,regarded among alternative plant sources of camptothecin.Camptothecin is a monoterpene indole anti-cancer alkaloid with annual trade value of over three billion U.S.dollars in the recent,and is used in the production of its analog drugs approved for the chemotherapy of cancer of varied types.Effects of plant growth regulators,culture media strength and photoperi-odic duration on the micropropagation ef ciency of C.fragrans from nodal segment explants were studied on Murashige and Skoog(MS)medium amended with Thidiazuron(TDZ),Benzylaminopurine(BAP)or Kinetin(Kin).Thidiazuron was more ef cient over BAP and Kin when half basal MS medium was used over full or quarter strength.Results of carbon source experiment showed sucrose as the most effective over glucose,fructose,and maltose in the clonal production.Studies on the photope-riodic incubation duration showed 12 h as the best light period and sub or supra-optimal resulted in the production of abnormal and albino micro shoots.Experimental results on the evaluation of physiological,biochemical parameters showed the role of pigment molecules and antioxidant systems in the production of albino micro shoots.  相似文献   

高山笃斯越桔离体快繁体系建立及种质试管保存研究   总被引：2，自引：0，他引：2  

顾地周  顾美影  曹逊  姜云天  朱俊义 《山东农业大学学报(自然科学版)》2009,40(2)

以高山笃斯越桔新生嫩芽为外植体,应用均匀设计法筛选其最适合的基部直接再生芽苗、生根及种质试管保存的培养基,结果表明,最适合的基部直接再生芽苗诱导培养基为:DR + TDZ2.0 mg·L-1 + IBA0.01 mg·L-1,分化率为98.8%以上;生根培养基:MS(改良) + IAA0.5 mg·L-1+IBA0.07 mg·L-1 + Kt0.1 mg·L-1,生根率达98%;试管保存培养基:1/5MS+B91.8 mg·L-1+根皮苷3.3 mg·L-1,保存时间可达43个月以上.以再生植株的茎节为材料进行快繁的结果表明,在35 d的一个培养周期内,增殖倍数平均达60以上.常温条件下,采取"低营养矮化延缓生长"的方法在试管内保存种质资源.建立了高山笃斯越桔的离体培养和种质试管保存体系.  相似文献   

转rolA、B、C基因枳橙快繁技术   总被引：5，自引：0，他引：5  

胡春华  谢玉明  黄训才  郭琛  焦徕  吴芯茹  邓子牛 《果树学报》2006,23(1):142-144,F0004

通过对转rol基因枳橙B、D、E系及对照的高接植株春梢茎段进行萌芽诱导、增殖及试管苗生根成苗的试验,摸索出适宜枳橙快繁各种培养基配方及培养条件。结果表明,以MS+6-BA1mg/L的培养基适合于芽萌发,以MS+6-BA0.5mg/L+NAA0.05mg/L的培养基适合于芽增殖,生根则以1/2MS+NAA0.5mg/L+0.2%活性炭的组合最佳,转基因各系生根率均在93.3%以上,显著高于对照生根率(66.7%)。快繁苗移栽成活率可达90%,目前生长良好。  相似文献   

柑桔三倍体苗的试管嫁接及微繁殖研究     

陈如珠  李耿光  张兰英 《中国南方果树》1992,(4)

甜橙胚乳三倍体试管苗茎段及顶芽采用试管扦插培养难于生根,亦不能正常生长;而将其茎段和顶芽嫁接于试管中的砧木上,成苗率达90％以上。在含BA0.1mg/L的MT培养基中,每株嫁接苗形成3～4个芽,可作试管快速繁殖。嫁接苗移植于无菌土中,成活率高达95％以上。田间二重接可加速三倍体小苗生长。本方法为解决柑桔试管苗移植问题提供了一条新途径。  相似文献   

美人指葡萄组培快繁技术研究   总被引：1，自引：0，他引：1  

吴丽敏  娄汉平 《辽宁农业科学》2006,(4):48-49

采用美人指葡萄秋季副梢的茎段为外植体,最佳的取材时间为9月中旬,适宜的基本培养基为B5 6-BA 2.0 mg/L IBA 0.5 mg/L 3%蔗糖 0.6%琼脂,pH值调至5.8~6.0,为美人指葡萄茎段离体培养的最佳启动萌芽培养基;B5 IBA 0.5 mg/L 3%蔗糖 0.6%琼脂,pH值调至5.8~6.0,为最佳增殖生根培养基,生根率达98%。试管苗根长3~4 cm时开始移栽,移栽成活率90%。  相似文献   

Effects of permanent magnetic fields on the proliferation of Phalaenopsis protocorm-like bodies using liquid medium     

Pham Thanh Van  Jaime A. Teixeira da Silva  Le Huy Ham  Michio Tanaka 《Scientia Horticulturae》2011

Magnetic fields (MFs) have been applied for the first time in orchid micropropagation. Protocorm-like bodies (PLBs) – approximately 3 mm in diameter – first derived from leaf segment culture of Phalaenopsis Gallant Beau ‘George Vazquez’, were subcultured every 2 months, and served as initial explants. The proliferation of Phalaenopsis PLBs in liquid medium in the Miracle Pack^® culture system was affected by the action of different intensities and polarities of MFs: 0.1, 0.15 and 0.2 Tesla (T) at North (N) and South (S) poles. The MF of 0.1 T – S resulted in the greatest fresh weight of regenerated PLBs. The average number of neo-PLBs formed per clusters in the PLB treated by MF: 0.1–0.2 T was decreased compared to the control exposed to natural MF (5 × 10⁻⁶ T). The proliferation of PLBs under 0.15 T – MF at both N and S poles for 2 and 7 weeks demonstrated that a longer duration of exposure to an MF of 0.15 T, regardless of the polarity, resulted in greater biomass of newly formed PLBs and smaller average number of newly formed PLBs. The S pole of MF had stronger effects on Phalaenopsis PLBs proliferation than the N pole did in all treatments.  相似文献