地图鱼迟钝爱德华氏菌病病原菌的鉴定及毒力基因的检测   总被引：3，自引：0，他引：3  

赵飞  邹为民  谭爱萍  罗理  姜兰  陆小萏 《大连水产学院学报》2007,22(6):403-408

从患病地图鱼Astronotus ocellatus肝脏组织中分离到菌株AO1,经人工感染试验证实其为致病菌。通过ATB Expression半自动细菌鉴定仪和常规生理生化特性的分析,初步鉴定该菌株为迟钝爱德华氏菌Ed-wardsiella tarda。测定菌株AO1的16S rRNA基因序列并进行分析,结果显示,该菌株与迟钝爱德华氏菌的同源性最高,达99.9%。因此,可鉴定该菌株为迟钝爱德华氏菌。根据迟钝爱德华氏菌的毒力基因菌毛亚基(fimA)基因序列设计引物,进行PCR扩增,得到383 bp序列,该序列与迟钝爱德华氏菌的fimA基因序列相似性达99.0%,进一步说明菌株AO1具有fimA基因,有一定的致病力。药物敏感试验结果表明,菌株AO1对阿米卡星、氧氟沙星、庆大霉素、新生霉素、氨苄西林、氨曲南、卡那霉素、头孢咪啉、诺氟沙星、环丙沙星、呋喃妥因、妥布霉素、氨苄青霉素13种抗生素较为敏感。  相似文献   

应用Dot-IGS快速检测牙鲆腹水病病原的研究     

朱壮春  白云  陈阳  张文丽  邢朝斌  吴鹏  白崇洲  李金龙  史相国 《安徽农业科学》2009,37(22):10539-10541

[目的]建立一种简便快速检测迟钝爱德华氏菌的斑点免疫金染色诊断方法。[方法]选用硝酸纤维素膜作固相载体,以胶体金标记羊抗兔IgG,根据棋盘试验,确定Et免疫血清和金标抗体最佳工作浓度,以出现明显清晰斑点者判定为阳性。[结果]迟钝爱德华氏菌呈阳性,温和气单胞菌、嗜水气单胞菌、河流弧菌、溶藻弧菌、鳗弧菌、腐败希瓦氏菌、产碱普罗威斯登菌、阪崎肠杆菌和大肠杆菌均呈阴性。[结论]Dot—IGS方法简便、特异、快速、结果直观,便于在基层推广使用。  相似文献   

加州鲈迟缓爱德华氏菌病的检测和防治     

宣云峰  沈勇亮  杨小猛  曹勇  顾泽茂 《水产养殖》2014,35(10):8-10

以江苏省吴江市八坼镇区域养殖场加州鲈鱼病鱼的细菌性病原体为研究对象。通过分离纯化,形态学观察,结合16S rRNA基因序列同源性检索,对该病原菌进行初步判断。同时进行药敏实验,了解该病原菌对各种抗生素的耐药性和敏感程度。研究结果:分离纯化得到2株纯菌株L2F和L2P2,革兰染色均呈红色,阴性杆状菌。16S rDNA序列分析,L2F和L2P2菌株与迟缓爱德华氏菌(Edwardsiella tarda)的同源性达99%。初步判断该菌为迟缓爱德华氏菌。药敏实验结果,该菌对磺胺异恶唑、链霉素和利福平等药物不敏感或耐药,对左氧氟沙星、诺氟沙星、恩诺沙星、强力霉素、氟苯尼考、头孢哌酮相对敏感。  相似文献   

Surface-exposed expression of Edwardsiella tarda EseB in live attenuated Vibrio anguillarum based on novel surface display systems     

Qiyao Wang  Zhao Yang  Qin Liu  & Yuanxing Zhang 《Aquaculture Research》2009,40(13):1459-1467

Live, attenuated Vibrio anguillarum strains can serve as vectors for the delivery of heterologous antigens for development of multivalent recombinant vaccines. Based on the outer membrane anchoring elements of V. anguillarum , we have previously constructed several efficient surface display systems Lpp-Omporf1, Lpp-OmpU, Lpp-Omp26La, Wza-Omporf1, Wza-OmpU and Wza-Omp26La. In this study, with these constructed surface display systems, a putative antigen protein EseB from pathogenic Edwardsiella tarda was successfully expressed on the surface of an attenuated V. anguillarum strain to get multivalent vaccine candidates. Further immune protection evaluation in zebra fish ( Danio rerio ) demonstrated that the V. anguillarum EseB-display strain AV/pW-26La-B could trigger full protection against V. anguillarum infection and early protection against E. tarda infection in the immunized fish. These results suggest that surface display of heterologous protective antigens in attenuated V. anguillarum could be used as a tool to develop potential V. anguillarum vector vaccine.  相似文献   

Occurrence of multiple antibiotic resistance and genotypic characterization in Edwardsiella tarda isolated from cage‐cultured hybrid red tilapia (Oreochromis sp.) in the Ping River,Northern Thailand     

Guoyi Niu  Dilok Wongsathein  Sukolrat Boonyayatra  Rutch Khattiya 《Aquaculture Research》2019,50(12):3643-3652

Edwardsiella tarda is a pathogen that causes edwardsiellosis in aquatic animals. The emergence of multiple antibiotic‐resistant strains makes antibiotic treatment difficult. This study aimed to investigate the antibiotic susceptibility patterns and the genotypic characterization of E. tarda isolated from cage‐cultured red tilapia in Thailand. A total of 30 isolates were identified as E. tarda using biochemical and molecular analysis. The disc diffusion method for testing antibiotic susceptibility showed all the isolates were resistant to colistin sulphate and oxolinic acid. High levels of resistance to amoxicillin, ampicillin, ceftazidime, oxytetracycline and sulphamethoxazole/trimethoprim were observed as well. The multiple antibiotic resistance index ranged from 0.25 to 0.92, indicating that these isolates had been exposed to high risk sources of contamination where antibiotics were commonly used. All the isolates carried the bla_TEM gene based on polymerase chain reaction (PCR). The tetA and sul3 genes were detected in 90% (27/30) and 26.7% (8/30) of the isolates respectively. Nine different genetic groups of isolates were obtained using enterobacterial repetitive intergenic consensus polymerase chain reaction (ERIC‐PCR). A correlation between genetic types and multiple antibiotic‐resistant patterns was found. These results highlight the potential risks of multiple antibiotic‐resistant isolates for humans and the environment.  相似文献   

Edwardsiellosis in farmed turbot,Scophthalmus maximus (L.), associated with an unusual variant of Edwardsiella tarda: a clinical,aetiological and histopathological study     

L Qin  J Xu  Y G Wang 《Journal of fish diseases》2014,37(2):103-111

During 2005 and 2010, a survey of edwardsiellosis on eight turbot, Scophthalmus maximus (L.), farms was conducted in China. This report presents the detailed results of the study on this disease. Diseased turbot displayed two distinct types of gross signs: black discoloration of the dorsal skin on the posterior portion of the body; and red cutaneous foci on the ventral side. Internally, the most pronounced clinical signs in all fish examined were enlarged kidneys. The causal agent of the disease was finally proved to be one species of bacterium that was identified as Edwardsiella tarda by physiological and biochemical tests, API 32E and 16S ribosomal RNA sequence analysis. It is noteworthy that unlike the commonly described E. tarda strains, the isolates in this study were non‐motile strains without flagella. A histopathological study revealed that E. tarda infection was systemic in turbot and that kidney showed the most significant pathological changes, including acute focal necrosis, an influx of macrophages and formation of granuloma. The most common histopathological characteristics of this disease are the proliferation of macrophage in various organs and formation of granuloma. In addition, this article also gave background information on the disease and presented the results of virulence tests with the E. tarda strain identified in this study.  相似文献   

水产动物6种主要病原菌与抗血清的免疫交叉反应   总被引：14，自引：0，他引：14       下载免费PDF全文

战文斌  齐继光  刘洪明  周丽  邢婧 《中国水产科学》2004,11(1):14-19

采用ELISA、试管凝集、Western-blot等方法,分析了鳗弧菌(Vibrio anguillarum)、哈维氏弧菌(V. harveyi)、溶藻胶弧菌(V. alginolyticus)、副溶血弧菌(V. paraheamolyticus)、迟钝爱德华氏菌(Edwardsiella tarda)和荧光假单胞菌(Psedomonas fluorescens)等水产养殖中主要病原细菌与抗血清之间的免疫交叉反应.结果表明,弧菌属细菌之间的交叉反应程度比较大,而与其他两属的细菌之间存在的交叉反应程度小,或不存在交叉反应;Western-blot分析结果显示,哈维氏弧菌、溶藻胶弧菌和副溶血弧菌抗血清分别与其他3种弧菌在分子量为135.6 kD和121.5 kD;95.6 kD,48.4 kD,39.2 kD和34.9 kD;55.1 kD的蛋白带处存在交叉反应,而这些分子量的蛋白带与其他两属的抗血清均不发生反应.  相似文献   

新疆昌吉州波斑鸨分布与数量考察初报   总被引：5，自引：4，他引：5  

高行宜  乔德录  熊义锋  赵斌  许可芬  姚军 《干旱区研究》1996,(1)

本文报道了波斑鸨（Ｏｔｉｓｕｎｄｕｌａｔａ＝Ｕｎｄｕｌａｔａｕｎｄｕｌａｔａ）在新疆昌吉州境内的分布和种群数量。昌吉州为我国波斑鸨的主要繁殖地，繁殖区由三片不连续的地区组成，总面积约１３４００ｋｍ２，界于Ｅ８８°３０’－９１°１５’，Ｎ４３°５７’－４５°之间。据１２条样带统计结果，平均栖息密度为０．０３２±０．００８２只／ｋｍ２，种群数量为４２８．８±１００．８８只，即３１９—５３９只。  相似文献   

Blood parameters in Nile tilapia (Oreochromis niloticus L.) spontaneously infected with Edwardsiella tarda   总被引：6，自引：0，他引：6  

A Ca&#;lan Karasu Benli  & Hijran Yavuzcan Yildiz 《Aquaculture Research》2004,35(14):1388-1390

  相似文献   

迟缓爱德华菌(Edwardasiella tarda)对小鼠和剑尾鱼的免疫保护试验   总被引：3，自引：0，他引：3  

熊清明  陆承平 《中国兽医学报》2002,22(3):251-253

为了评价迟缓爱德华菌 (Edwardasiella tarda)的免疫保护效果 ,用致病性 E.tarda JEL4的全菌灭活物 (FKC)和胞外产物 (ECP)分别 2次免疫小鼠 15 0只和 1次免疫剑尾鱼 30 0尾 ,7周后用同源菌株和异源菌株进行攻击。结果显示 ,除 ECP组对某一异源菌株为 4/ 5保护外 ,免疫小鼠 FKC组和 ECP组全部获得保护 (5 / 5 )。在免疫的剑尾鱼中 ,FKC和 ECP组对同源菌株保护率分别为 6 0 %和 10 0 % ;对异源菌株的攻击 ,均不能全部保护 ,但 ECP组可明显延缓剑尾鱼死亡时间  相似文献