排序方式： 共有13条查询结果，搜索用时 15 毫秒
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[目的]分离和鉴定溶藻细菌,研究其溶藻特性,为进一步研究溶藻细菌对水华的治理作用提供帮助。[方法]从香溪河春季水华集聚区水体中分离得到1株有高效溶藻效果的菌株(H5),采用16S rDNA序列相似性分析和Biolog微生物自动鉴定系统等对细菌进行鉴定。采用直接计数法,研究了其对汉斯冠盘藻(Stephanodiscus hantzschii)、倪氏拟多甲藻(Peridiniopsis niei)、具尾逗隐藻(Komma cau-data)的抑制效果,及对汉斯冠盘藻的溶藻作用方式。[结果]根据生理生化及16S rDNA序列分析鉴定,H5属于纺缍形赖氨酸芽孢杆菌(Lysinibacillus fusiformis)。该菌对汉斯冠盘藻、倪氏拟多甲藻、具尾逗隐藻的溶藻率最高为71.3%,最低为57.4%。培养滤液、热处理培养滤液对汉斯冠盘藻的生长具有明显抑制作用,而细菌无细胞提取物无溶藻能力。[结论]该菌对汉斯冠盘藻有较强的溶藻效果,且是通过分泌溶藻物质溶藻。 相似文献
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为解决羊栖菜养殖生产需要的优良种苗,经过10多年努力,采用有性繁殖方式,进行定向选育,获得羊栖菜"鹿丰1号"。该优质羊栖菜的主要性状是:枝叶粗壮繁茂、气囊产生早、颗粒大、产量高,经过8年的养殖及检验,目标性状表现稳定,附苗总面积达到1912m2,培育出苗59.3hm2,总产量383.746t,平均产量6471kg/hm2。目前,该选育的羊栖菜已在温州地区的洞头县和苍南县以及山东部分海域进行了养殖中试,选育性状表现稳定,养殖产量高,为羊栖菜良种化的实现打下基础。 相似文献
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用自然感染的中国水牛食道上的梭形肉孢子虫(Sarcocystis fusiformis)的包囊喂猫6只、犬5只、仔猪2头及3名自愿受试者。包囊大小为6—17×2—7mm(平均11.64×3.84mm)。所有试验猫均排出孢子囊,而犬、猪及自愿受试者未发现孢子囊或卵囊。猫的潜隐期为7—13天。开放期为61—69天以上。其中有一只猫试验结束时继续排出孢子囊,未见任何症状。孢子囊大小为7.8—13×6.5—7.8μm(平均为10.6×6.8μm),内含4个子孢子及一个残体,无微孔及斯氏体,通常是单个的,偶而成对。 以人工感染猫的孢子囊分别喂犊水牛3头和小乳公牛1头,未见任何症状。第50天解剖其中1头犊水牛,其心、肝、肾及食道周围淋巴结有明显病理变化,并发现虫体。小乳牛既无病变,亦未发现虫体。 相似文献
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一株石油降解菌Lysinibacillus fusiformis 23-1的筛选鉴定及原油降解特性 总被引：1，自引：0，他引：1
石油泄露造成了严重的环境问题和重大经济损失,微生物修复是解决石油污染的最为有效的途径。从青藏高原石油污染土样筛选产生物表面活性剂的低温石油降解菌,并研究了该菌株对石油的乳化性能、降解特性、降解条件以及对不同碳链烃类的利用。本实验用血平板法分离到一株产表面活性剂石油降解菌23-1,形态学和16S rRNA基因序列分析鉴定其为纺锤形赖氨酸芽孢杆菌(Lysinibacillus fusiformis 23-1),比色法测定该菌株的乳化性能为19.6%,超声波法测定乳化稳定性为37.5%,表明其能够降低油水界面张力,具有增溶作用。质量法测定L. fusiformis 23-1对石油的降解率为57%,适宜降解条件为:初始pH为7.5,温度为25 ℃,培养8 d后获得最佳降解效果。GC-MS方法测定该菌株的石油降解特性,结果表明,该菌对石油中不同碳链的烃类降解能力不同。L. fusiformis 23-1能产表面活性剂,具有较强的降解石油能力,可用于石油污染修复。 相似文献
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羊栖菜硫酸多糖的抗凝血活性研究 总被引：1，自引：0，他引：1
[目的]探讨羊栖菜硫酸多糖的抗凝血活性。[方法]从水提液中提取羊栖菜硫酸多糖,通过体内和体外的抗凝血试验系统地研究其抗凝血活性。[结果]利用葡聚糖凝胶G-75分离纯化所提取的羊栖菜硫酸多糖F1,得到5个不同组分。在2.0 g/L的受试条件下,组分F12抗凝血活性的最强,其延长小白鼠的出血时间和凝血时间、大白兔的凝血活酶时间、凝血酶时间、凝血酶原时间分别为粗多糖F0的174.2%、153.7%、311.9%1、57.8%和218.2%。羊栖菜硫酸多糖F1与抗凝血效果有显著的量效关系。在受试条件下(浓度为0.5~2.0g/L),多糖的浓度与小白鼠的抗凝血指标、与大白兔体外抗凝血指标之间均呈现良好的直线回归关系。[结论]该研究为羊栖菜类保健品及临床药物的开发提供理论基础。 相似文献
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Ketan V. Tank Thresiamma James C. Mohanakumaran Nair Viral C. Bajaniya 《Journal Of Applied Aquaculture》2013,25(2):109-116
The cyanobacterium Arthrospira fusiformis (AF) has a high protein content of 60%-65% and is rich in vitamins, minerals, fatty acids (gamma-linolenic acid), carotenoid, and other antioxidant pigments. A 60-day feeding trial was conducted using postlarval Penaeus monodon (0.041 g mean initial weight) to evaluate the efficacy of six isonitrogenous (40% protein) test diets containing various inclusion levels of AF. Clam meat was replaced with AF at an inclusion level of control, 5%, 10%, 20%, 30%, and 40%. The diet with 20% AF produced a significantly greater weight gain, specific growth rate, food conversion ratio, and protein efficiency ratio than all other diets (p<0.05). Further increase in the AF inclusion levels significantly decreased growth and feeding performance. Percentage survival and water stability of diet was significantly higher at inclusion levels of 10% and 20%. The results suggest that AF cannot serve as the sole protein source in the diet of P. monodon postlarvae, but can be effectively used as a supplementary protein source. 相似文献
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Shao Jun Pang Ti Feng Shan Zhi Huai Zhang Jian Zhang Sun 《Aquaculture Research》2008,39(13):1408-1415
Mariculture of the brown alga Hizikia fusiformis (Harvey) Okamura as an export‐oriented human food has been there more for than 20 years in China. It is now one of the five major farmed algal species along the Chinese coast. Stable and sufficient supply of young seedlings for scaling up the cultivation has been a problem throughout the farming history of this species due to the unique dioecious life cycle and relatively short time window of sexual reproduction in nature. These two factors led to a practical difficulty in obtaining zygotes at identical developmental stage in viable amounts for seedling production. A key solution to this problem is to control the synchronization of the receptacle development and to realize the simultaneous discharge of male and female gametes, such that the fertilization rate could be greatly enhanced. Focusing on one of the farmed populations in this report, we present our results on mass production of seedlings using the synchronization technique on a large scale performed in 2007. Totally 5.5 hundred million embryos were obtained from 100 kg female sporophytes. The seedlings were raised up to 3.5 mm in length in greenhouse tanks over a month and were further grown in open sea for over 3 months at two experimental sites. The success of mass production of seedlings in this alga helped to lay the basis for future trials in other species in the genus of Sargassum that have identical life cycle. 相似文献
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Effectiveness of Ethrel as a Male Gametocide in Pearl Millet and its Influence on Ergot 总被引：1，自引：0，他引：1
In field and greenhouse experiments Ethrel (2-chloroethyl phosphonic acid) was tested for its male garnetocidal effects on pearl millet (Pennisetum americanum) and its subsequent effects on ergot development. Application of Ethrel at 2000 ppm at late boot or early protogyny was the most effective for inducing male sterility in the hybrid, EJ 104. Female fertility in a male sterile line, however, was not affected by Ethrel treatment. Ethrel at 2030 ppm applied at ihe late boot stage resulted1 in partial paniele exsertion, and reduced plant height anc. panide length. In vitro Ethrel (2000 ppm) completely inhibned pollen germination but did not affect germination of conidia of Claviceps fusiformis, the causal agent of ergot of pearl millet. Ergot resistance or susceptibility in pearl millet lines was not affected., probably because Ethrel could not induce complete male sterility. 相似文献