Changes of Ca^2＋ activated potassium channels and cellular-proliferation in autogenous vein grafts     

Changes of Ca  SONG Sheng-yun  MA Bao-an  FAN Qing-yu 《园艺学报》2004,20(4):575-579

AIM: To investigate changes of Ca^2＋ activated potassium channels (K_Ca) in autogenous vein grafts. METHODS: The contraction of venous ring was measured by means of perfusion in vitro. The intimal proliferation and proliferation of cultured smooth muscle cells (vascular smooth muscle cells, VSMCs) were observed by the means of computerised image analysis and MTT method, respectively. Furthermore, whole cell mode of patch clamp was used to record K_Ca of VSMCs isolated from autogenous vein grafts. RESULTS: 1 week after transplantation there were no significant differences of contraction and intimal relative thickness between autogenous vein grafts and control. Contraction and intimal relative thickness of autogenous vein graft were significantly increased 2 weeks after transplantation (P＜0.05, n=8 vs control), and they were more enhanced 4 weeks after vein transplantation (P＜0.01, n=8 vs control). TEA (blocker of Ca^2＋ activated potassium channels) increased MTT A₄₉₀ value of VSMCs from femoral vein in a dose-dependent manner (P＜0.05, n=8). K_Ca current density was significantly attenuated in VSMCs from autogenous vein grafts 1-4 weeks after transplantation (P＜0.05, n=5). CONCLUSION: K_Ca was inhibited in autogenous vein graft, which accounted for vasospasm and intimal proliferation.  相似文献   

不同基质活性炭对阿特拉津吸附特性研究     

杨峰山  孙丛  于苹艳  马玉堃  付海燕  刘春光 《中国农学通报》2020,36(12):68-72

为了解决阿特拉津给土壤带来的污染问题以及寻找吸附效果较好的活性炭基质,本研究利用高效液相色谱(HPLC)技术检测了阿特拉津溶液和悬浮液经煤、木、果和竹质4种生物炭处理后的残留量,并对其在阿特拉津溶液和土壤中的吸附动态进行检测。结果表明,煤质活性炭对初始浓度为100 mg/L和10 mg/L的阿特拉津溶液和悬浮液的吸附效果较好,其吸附率为5.651%~68.42%;当阿特拉津初始浓度为100 mg/L时,煤质活性炭在40 min时对阿特拉津溶液的吸附率高达23.49%;与对照组相比,当阿特拉津的初始浓度为100 mg/L时,土壤中阿特拉津经煤质活性炭处理42天后的残留浓度最低,活性炭吸附率达到91.39%。综上,煤质活性炭能有效降解溶液及土壤中阿特拉津含量,这将为阿特拉津污染土壤的改良研究奠定基础。  相似文献   

Effects of water‐misting sprays with forced ventilation on post mortem glycolysis,AMP‐activated protein kinase and meat quality of broilers after transport during summer          下载免费PDF全文

Nannan Jiang  Tong Xing  Minyi Han  Shaolin Deng  Xinglian Xu 《Animal Science Journal》2016,87(5):718-728

Effects of water‐misting sprays with forced ventilation on post mortem glycolysis, adenosine monophosphate‐activated protein kinase (AMPK) and meat quality of broilers after transport during summer were investigated in the present paper. A total of 105 mixed‐sex Arbor Acres broilers were divided into three treatment groups: (i) 45 min transport without rest (T); (ii) 45 min transport with 1 h rest (TR); and (iii) 45 min transport with 15 min water‐misting sprays with forced ventilation and 45 min rest (TWFR). Each treatment consisted of five replicates with seven birds each. The results indicated that the water‐misting sprays with forced ventilation could mitigate the stress caused by transport under high temperature conditions during summer, which reduced the energy depletion in post mortem Pectoralis major (PM) muscle. This resulted in a higher energy status compared to the T group, which would decrease the expression of phosphorylation of AMPK (p‐AMPK). Furthermore, decreased the expression of p‐AMPK then slowed down the rate of glycolysis in post mortem PM muscle during the early post mortem period, which in turn lessened the negative effects caused by transport on meat quality. In conclusion, water‐misting sprays with forced ventilation may be a better method to control the incidence of the pale, soft and exudative meat in broilers.  相似文献   

Molecular mechanisms of suberoylanilide hydroxamic acid in the inhibition of TGF‐β1‐mediated canine corneal fibrosis          下载免费PDF全文

Kristina M. Gronkiewicz  Elizabeth A. Giuliano  Ajay Sharma  Rajiv R. Mohan 《Veterinary ophthalmology》2016,19(6):480-487

  相似文献   

Cyclooxygenase (COX) inhibitors and the intestine   总被引：1，自引：0，他引：1  

Little D  Jones SL  Blikslager AT 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2007,21(3):367-377

Nonsteroidal anti-inflammatory drugs (NSAIDs) have long been used for the treatment of pain and inflammation because of their inhibitory effects on cyclooxygenase (COX). For almost as long as NSAIDs have been in use, multiple adverse effects have been noted. Assessment of many of these adverse effects have been complicated because of the discovery of multiple splice variants of the cox gene, and a greater array of COX inhibitors, especially the COX-2 selective inhibitors have become available. Some of these adverse effects cannot be readily explained by the effect of these drugs on COX. This has sparked a new field of investigation into the COX-independent effects of the COX inhibitors. The major noncyclooxygenase targets of the COX inhibitors of particular relevance to inflammation and the gastrointestinal tract are phosphatidylinositol 3'-kinase Akt signaling, uncoupling of oxidative phosphorylation, PPARgamma, nuclear factor KB, mitogen activated protein kinases, and heat shock proteins.  相似文献   

时间和温度对活性炭与板栗壳吸附铜锌铅镉的影响     

段孟辰  薛科社  邹卓彪 《农业环境与发展》2013,(5)

随着工业化和城市化进程的加快,废水排放引起的水环境重金属污染问题越来越突出,去除污水中的重金属成为水环境保护的重要内容之一。本文选取活性炭与板栗壳作为吸附剂,研究在不同的时间及温度下,这2种吸附剂对废水中的Cu2+、Zn2+、Pb2+、Cd2+的吸附性能影响。研究发现,活性炭作为吸附剂时,Cu2+吸附量最大是在0.5 h时,Zn2+是在3 h时,Pb2+和Cd2+则是1 h时;用板栗壳吸附时,Cu2+和Pb2+吸附量最大在1 h,Zn2+是在1 h时,Cd2+则是0.5 h时。吸附剂为活性炭或板栗壳,温度对Zn2+的吸附率影响都不大;吸附Cu2+时,活性炭受温度的影响大于板栗壳,其吸附率随温度的升高而减小;Pb2+和Cd2+的吸附受温度影响明显,均是40℃时吸附率较高,但板栗壳>活性炭,且Cd2+>Pb2+。  相似文献   

磁化或电离化微咸水理化特性试验   总被引：4，自引：2，他引：4  

王全九  张继红  门旗  谭帅  周莉薇  刘湘岩 《农业工程学报》2016,32(10):60-66

改善微咸水理化特性,提高微咸水利用效率,成为合理利用微咸水重要研究内容。为了对微咸水活化效果及活化微咸水理化特性进行定量评价,该文采用磁化和电离化法对微咸水进行不同的活化处理,通过室内测试,对电离化微咸水活化系统的技术参数进行了分析,并对活化微咸水的表面张力系数、溶氧量、电导率及p H值等理化特性进行了研究。结果表明,电离化系统要求接地电阻不超过5Ω;活化微咸水表面张力系数显著减小、溶氧量显著增加、电导率和p H值受到不同程度的影响;但对表面张力系数和溶解氧影响比较明显,对电导率和p H值影响不显著。在矿化度5 g/L时,活化微咸水表面张力相对减少9.14%~13.84%,溶氧量相对增加8.04%~10.23%,表面张力相对减少量与溶氧量相对增加量可以从客观上反应活化微咸水理化特性的变化,而表面张力相对减少量与溶氧量相对增加量之间又存在显著的指数函数关系。根据表面张力相对减少量,电离化微咸水活化处理效果优于磁化处理,磁场强度3 000 Gs与变频磁化处理活化效果显著。因此,可以用表面张力相对减少量作为定量评价指标对活化微咸水的理化特性进行综合性定量评价,以判断不同活化方式的活化效果。  相似文献   

活性污泥应用于农业前发酵处理过程中病原菌变化研究     

穆国俊 魏静王殿武  李卫国孟静 《华北农学报》2007,22(B08):38-42

采用黑曲霉为发酵剂对活性污泥进行全程无公害处理并对各种病原菌的生长变化进行监测。结果表明病原菌总量在发酵过程中呈“马鞍型”变化。由于不同堆肥处理组合的C/N比及添加辅料的不同,导致各种病原菌的生长规律出现差异;采用黑曲霉处理城市活性污泥可使病原菌数目减少,但不能达到完全杀灭的目的;对发酵污泥进行200℃高温物理处理后,病原菌存活量为零。因而活性污泥在堆肥后必须经过高温物理处理才能达到安全化和资源化。  相似文献   

化学强化初沉污泥厌氧消化产甲烷潜力研究     

肖丽君  马向伟  王永生  都的箭  吴晴  熊蒂程 《中国沼气》2022,(1):18-23

该试验以化学强化初沉污泥为研究对象,采用中温/高温(35℃/55℃)厌氧消化方法,研究采用化学一级强化处理工艺(CEPT)产生的化学强化初沉污泥厌氧消化产甲烷效果,并对比化学强化初沉污泥在中温、高温工况的厌氧消化产甲烷性能,明确适合化学强化初沉污泥的处理工艺.研究结果表明,化学强化初沉污泥单位VSS累积产甲烷量和VSS...  相似文献   

高锰酸钾复合药剂与粉末活性炭联用组合对微污染源水的强化混凝效能   总被引：8，自引：0，他引：8  

池秀静  魏晓妹  李洪源 《水土保持通报》2002,22(1):10-13

通过对高锰酸钾复合药剂、粉末活性炭、预氯化工艺的对比及它们之间的联用组合工艺的试验研究 ,证明高锰酸钾复合药剂与粉末活性炭联用组合对微污染水的除色、除味、降低出水浊度效果明显。从而为老水厂寻找了一种简便易行、经济有效的去污染手段。  相似文献