品质不同的稻米胚乳细胞形态特征的扫描电镜观察   总被引：5，自引：0，他引：5  

瞿波  徐运启 《华中农业大学学报》1991,10(4):404-408,T002

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松树种子胚乳DNA的抽提与分析     

文亚峰  谭晓风 《中南林业科技大学学报(自然科学版)》2004,24(4):20-23

运用改良SDS(十二烷基硫酸钠)法,从马尾松、黄山松、黑松等松树种子胚乳中提取到较高纯度的DNA,并对其纯度、浓度及产率进行了分析.经RAPD检测表明,DNA扩增效果良好,完全能满足常规DNA实验的要求,为针叶树种等其它小粒种子DNA的提取提供了经济、快速、可靠的实验方法.  相似文献   

Physicochemical Property Analysis and Gene Mapping of a Floury Endosperm Mutant flo7 in Rice     

FANG Peng fei  LI San feng  JIAO Gui ai  XIE Li hong  HU Pei song  WEI Xiang jin  TANG Shao qing 《中国水稻科学》2014,28(5):447

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基于透射光和反射光图像同位分割的小麦品种识别方法研究     

闫宁  张晗  董宏图  康凯  罗斌 《浙江农业学报》2022,34(3):590-598

为提高基于机器视觉的小麦品种识别准确性,本文通过透射光和反射光同位图像分割对种子颜色特征参数进行了优化提取.采用透射光图像辅助反射光图像分割的方式从种子图像中分割出胚部区域,并分别提取小麦整粒、种胚、胚乳区域的颜色特征参数.以济麦22、济麦44、京麦9、京麦11共4个品种种子作为研究对象,利用HALCON机器视觉软件获...  相似文献   

Protein Phosphorylation and Protein Patterns in Developing Endosperms of Barley     

T. Yupsanis  P. R. Shewry 《Plant Breeding》1993,111(1):73-77

The SDS-soluble protein-fractions of barley endosperms (2 and 3 weeks after anthesis) were compared using 1-D SDS-PAGE and 2-D IEF/SDS-PAGE. Similar procedures have been followed after in vivo phosphorylation of isolated endosperm with ³²PO₄.Protein patterns were similar when 1-D SDS-PAGE was used. On the other hand, 2-D IEF/SDS-PAGE revealed several major differences, the most important being the appearance of a major protein of Mr about 40,000 in the 3-weeks old endosperm. By contrast, the patterns of phosphorylated proteins, with both methods, were relatively simple. Only two phosphorylated bands showed up in the 3-weeks old endosperm: a highly labelled band of Mr about 65,000, and a band of about 75,000. In the case of 2-weeks old endosperm, where the phosphorylation was more active, these and several others were also labelled, but the Mr 75,000 band was labelled more intensely.  相似文献   

Interspecific hybridization of a multiploid mutant of durum wheat with rye and Triticum monococcum L. results in pentaploid hybrids     

D. L. Klindworth  N. D. Williams  R. A. McIntosh 《Plant Breeding》2003,122(3):213-216

The multiploid mutant of durum wheat is a genotype that produces unreduced gametes. Our objective was to test the recovery of pentaploid hybrids in crosses of the mutant with rye and Triticum monococcum L. Compared with check crosses, the mutant had a two‐third reduction in percent seed set for rye crosses, but had only a slight decrease in crossability with T. monococcum. Pentaploid hybrids were associated with plump seeds of the mutant/rye cross, and with shrivelled seeds of the mutant/T. monococcum cross. We suggest that the endosperm balance number hypothesis explains the association of pentaploid hybrids with endosperm type. This association made for easy recovery of pentaploid hybrids from crosses to both species. Mature, plump seeds from the mutant/rye cross were germinated and pentaploid hybrids were recovered. One pentaploid hybrid was recovered for every 50.5 and 15.1 florets pollinated with rye and T. monococcum, respectively. Unreduced gametes in the multiploid mutant will facilitate interspecific hybridization by reducing the time to produce pentaploid plants.  相似文献   

Wheat starch biosynthesis     

M.K. Morell  S. Rahman  A. Regina  R. Appels  Z. Li 《Euphytica》2001,119(1-2):55-58

Starch biosynthesis in plants involves the concerted action of a numberof enzymes, including ADPglucose pyrophosphorylase, starch synthases,branching enzymes and debranching enzymes. We report on the cloningand characterisation of genes encoding these enzymes from wheat and ontheir chromosomal locations. The prospects for manipulating wheat starchstructure and functionality using these genes is discussed.  相似文献   

稻米胚乳直链淀粉含量的基因剂量效应   总被引：4，自引：0，他引：4  

孙义伟  刘宜柏 《江西农业大学学报》1989,(1)

用不同直链淀粉含量的品种杂交获得的 F_1、F_2及亲本为材料,测定了单个胚乳的直链淀粉含量,以研究稻米胚乳直链淀粉含量的遗传。结果表明,蜡质位点的遗传由1个主效基因控制,F_1和 F_2的直链淀粉含量存在 Wx 基因剂量效应,有2个 Wx 等位基因对直链淀粉合成起着不同的作用,分离世代出现超亲个体,说明还存在修饰基因作用。  相似文献   

利用三倍体胚乳遗传模型定位爆裂玉米膨爆特性QTL   总被引：6，自引：0，他引：6  

李玉玲  董永彬  崔党群  牛素贞  王延召  余永亮 《中国农业科学》2006,39(3):448-455

 【目的】探讨爆裂玉米膨爆特性的分子遗传基础，为遗传改良和分子标记辅助选择提供有益信息。【方法】以普通玉米自交系丹232和爆裂玉米自交系N04为亲本构建259个F2﹕3家系群体，利用SSR标记构建分子标记遗传图谱，利用三倍体胚乳遗传模型和区间作图法对F2﹕3群体在两种环境条件下的膨爆特性进行了QTL定位和效应分析。【结果】构建的爆裂玉米SSR遗传连锁图谱包含183个标记，覆盖玉米基因组1762.2 cM，标记间平均距离为9.63 cM。爆裂玉米的膨爆特性是由效应不等的多基因控制的数量性状，同时受环境条件的影响。两种环境条件下共检测到18个爆花率QTL，17个膨化体积QTL和21个膨化倍数QTL。单个QTL可解释的表型变异介于4.4%～28.0%，可解释的表型总变异为65.8%～96.6%，基因的作用方式为加性、部分显性、显性和超显性的QTL数目分别为2、1、4、20和1、5、1、22，超显性在膨爆特性的遗传中起着最重要的作用。【结论】与以往膨爆特性以加性效应遗传为主的研究结果不一致的主要原因在于三倍体胚乳模型对显性效应的细分和对显性效应较小QTL的显性效应的高估。  相似文献   

水稻胚乳细胞计数方法研究   总被引：10，自引：0，他引：10  

张祖建  朱庆森  王志琴 《扬州大学学报(农业与生命科学版)》1996,(2)

在改进Ｓｉｎｇｈ小麦胚乳细胞计数方法的基础上，研究提出水稻单个籽粒胚乳细胞的计数方法，进行了细胞计数过程中各环节的误差分析，并对计数方法鉴别样品差异的能力做出统计推断。证明是一种可用于较大样本量、简便易行的水稻胚乳细胞计数方法。  相似文献