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Yue-peng Song Xi-bing Jiang Man Zhang Ze-liang Wang Wen-hao Bo Xin-min An De-qiang Zhang Zhi-yi Zhang 《中国林学(英文版)》2012,14(1):1-7
We analyzed the genetic differences of 16 poplar clones between genomic-SSR and EST-SSR markers.The statistical results show that the average number of alleles detected by genomic-SSR was 4.1,Shannon’s index 1.0646,observed heterozygosity 0.4427 and expected heterozygosity 0.5523,while for the EST-SSR,the average number of alleles was 2.8,Shannon’s index 0.6985,observed heterozygosity 0.2330 and expected heterozygosity 0.4684.Cluster analysis indicated that the EST-SSR capacity of genotypic identification was more precise than that of genomic-SSR.These results reveal that EST-SSR and genomic-SSR have statistically significant genetic differences in polymorphism detection and genotypic identification.These differences could provide a theoretical basis for the rational use of SSR markers in species diversity and other related research. 相似文献
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为探讨Genomic-SSR与EST-SSR标记在桉树种间的遗传差异,本研究利用两种不同来源的SSR标记对6种桉树进行遗传差异性分析.分析结果表明:Genomic-SSR标记在6种桉树中具有高的遗传多样性,EST-SSR标记在揭示不同基因型间遗传关系方面更具优势.对两种标记进行聚类分析发现,EST-SSR标记比Genomic-SSR标记能更准确地鉴别基因型.两种不同来源SSR标记的遗传差异性,为更有效利用SSR标记在桉树遗传多样性分析、种质鉴定等相关研究提供了理论支持. 相似文献
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Simple sequence repeat (SSR) markers have been shown to be a powerful tool for varieties identification in plants. However, SSR fingerprinting of sweetpotato varieties has been a little reported. In this study, a total of 1294 SSR primer pairs, including 1215 genomic-SSR and 79 expressed sequence tag (EST)-SSR primer pairs, were screened with sweetpotato varieties Zhengshu 20 and Luoxushu 8 and their 2 F1 individuals randomly sampled, and 273 and 38 of them generated polymorphic bands, respectively. Four genomic-SSR and 3 EST-SSR primer pairs, which showed good polymorphism, were selected to amplify 203 sweetpotato varieties and gave a total of 172 bands, 85 (49.42%) of which were polymorphic. All of the 203 sweetpotato varieties showed unique fingerprint patterns, indicating the utility of SSR markers in variety identification of this crop. Polymorphism information content (PIC) ranged from 0.5824 to 0.9322 with an average of 0.8176. SSR-based genetic distances varied from 0.0118 to 0.6353 with an average of 0.3100 among these varieties. Thus, these sweetpotato varieties exhibited high levels of genetic similarity and had distinct fingerprint profiles. The SSR fingerprints of the 203 sweetpotato varieties have been successfully constructed. The highly polymorphic SSR primer pairs developed in this study have the potential to be used as core primer pairs for variety identification, genetic diversity assessment and linkage map construction in sweetpotato and other plants. 相似文献
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