Selection and amplification of the liver stem cell subset from rat bone marrow cells with a medium containing cholestatic serum in vitro     

CAI Yun-feng  MIN Jun  FANG Tian-ling  CHU Zhong-hua  DENG Xiao-geng  HE Jing-song  CHEN Ji-sheng 《园艺学报》2003,19(8):1029-1033

AIM: To explore the feasibility of direct separat and selective enlargement of the bone marrow-derived liver stem cells (BDLSC) from bone marrow cells with a culture system containing cholestatic serum in vitro. METHODS: Bone marrow cells of rats were cultured with selective media containing 2%, 5%, 7% and 10% cholestatic rat serum, respectively. The BDLSC were then induced to proliferate with the addition of hepatocyte growth factor (HGF) on the firth day. BDLSC were characterized using immunocytochemistry and RT-PCR for lineage markers, glycogen staining and urea synthetic assay for functions 2 weeks later. RESULTS: Bone marrow cells were unble to form colony in the presence of 2% cholestatic serum and apopotosis appeared gradually in 7% or 10% cholestatic serum. The BDLSC survived in the medium containing 5% cholestatic serum while the other types of cells did not. The survival cells proliferated with a high speed during the second week and then formed hepatocyte-like colony-forming units (H-CFU). Cells in the H-CFU expressed the characteristic proteins of fetal hepatocytes. Furthermore, they had glycogen storage and urea synthesis functions, two of the critical features of hepatocytes. CONCLUSION: The selective micro-environment effectively selected BDLSC from the bone marrow cell, and will be a new way to provide an abundant source of donor hepatocytes for clinical cell therapy.  相似文献   

湖南猪致病性大肠杆菌的分离及药物敏感性监测   总被引：7，自引：0，他引：7  

黎满香  宁玲忠  董伟 《畜牧兽医杂志》2005,24(1):6-8

从湖南47个规模化猪场发生典型仔猪黄、白痢猪只的63份腹泻病猪分离出63株大肠杆菌，其中12株作致病力试验均有致病性，对25种抗菌药物药敏试验，大肠杆菌对胺卡那霉素和头孢唑啉敏感，敏感菌株分别占98．41％和92．06％；多种抗生素产生耐药性，且为多重耐药。  相似文献   

TFSQ型高效环保组合式粮食杂质清理筛的研制     

毛根武  杨健  周清伟  董德良  肖红  向飞 《粮食储藏》2014,(2):50-53

通过对振动筛理、垂直风选、旋风分离与沉降系统的研究,研制出集振动筛理、风选、旋风分离沉降、含尘气流循环再利用为一体的TFSQ型高效环保组合式粮食杂质清理筛。经应用测试和专家鉴定:该设备具有清理效率高、清理过程粉尘污染小,使用维护方便、可靠性强等优点,具有广阔的市场推广前景。  相似文献   

江浙地区葡萄霜霉病菌的致病性差异研究     

张眉  郭霞  吴斌  姜珊珊  王升吉  杨万海  辛相启 《中国农学通报》2020,36(31):103-109

为明确江苏、浙江不同地区葡萄霜霉病菌的致病性分化情况,采用单斑分离法和叶盘接种法,对分离得到的不同葡萄霜霉病菌单斑菌株进行致病性测定。结果表明,分离获得的80个葡萄霜霉病菌株都有致病性,且2个省份均有半数以上的菌株的病情指数在40~60之间。致病力聚类分析表明,2个省份的菌株都可以聚类为强、中、弱3种致病力类型,江苏以强致病力菌株为优势菌株,浙江以中等致病力菌株为优势菌株。对同一省份的菌株进行致病力分析发现,江苏树山和浙江永福的菌株致病力明显强于同省其他地区,而江苏新坊和浙江上钱的菌株与同省其他地区相比,致病力最弱。同时,构建菌株的系统发育树,结果显示,同源性相同的菌株间有致病力不同的情况,致病力相同的菌株间也存在rDNA-ITS序列有差异的情况。综上,江苏、浙江的葡萄霜霉病菌都存在致病力分化现象,这种分化现象与菌株的亲缘关系远近没有相关性,但同一省份不同地区间的菌株致病力差异与菌株的地理来源有一定的相关性。  相似文献   

诱导Paracin1.7生成的刺激因子的分离与纯化     

田甜  孙艳阳  康杰  宋刚  高冬妮  葛菁萍 《中国农学通报》2020,36(32):17-22

旨在分离纯化枯草芽孢杆菌(Bacillus subtilis)中能够诱导细菌素Paracin1.7分泌的刺激因子,并探明其对副干酪乳杆菌(Lactobacillus paracasei) HD1.7细菌素生成量及群体感应相关基因luxS表达的影响。本研究通过60%硫酸铵沉淀、CM Sepharose Fast Flow弱阳离子交换柱层析中度纯化及Superdex 75凝胶层析精度纯化,将获得的层析分离物与L. paracasei HD1.7共培养,检测共培养发酵液的抑菌活性及luxS基因的转录水平,并用SDS-PAGE与Native-PAGE检测分离物质。结果表明L. paracasei HD1.7抑菌活性为126.68%;luxS基因上调表达,为对照菌株的2.43倍;刺激因子的表观分子量约为30 kDa。本研究利用三步法初步分离纯化出诱导Paracin1.7生成的刺激因子,明确该刺激因子可以启动种间群体感应相关基因luxS,为L. paracasei HD1.7的群体感应研究奠定了理论和技术基础。  相似文献   

葡萄种植中土石分离混料机的设计——基于TRIZ理论     

赵威  尚欣 《农机化研究》2016,(11):166-170,176

针对宁夏贺兰山东麓葡萄种植基地,葡萄种植定值沟的挖掘作业步骤繁琐、耗费时间长,劳动强度大、工作效率低等现状,基于TRIZ理论并运用技术矛盾冲突设计了土石分离混料机。该机是集土石分离、土壤与农家肥混合并回填定植沟等作业同步进行的机械设备,彻底改变了人工捡石、施肥、旋耕、机械挖掘等传统的作业模式,大大节省了人力、物力,并提高了葡萄种植定植沟挖掘作业的工作效率。  相似文献   

三峡大学校园垃圾分类回收现状及回收机制的建立探讨     

徐志高  尹忠芹  汪佳其  尹家奇  高金芳 《绿色科技》2011,(10)

指出了垃圾分类回收具有重要的意义，有利于资源节约型和环境友好型社会的建设，有利于循环经济的可持续发展。分析了校园垃圾分类回收现状，对建立校园分类回收机制进行了探讨。  相似文献   

利用固液分离技术对规模化奶牛场的粪污治理     

张嫚  翟中葳  张克强  刘福元  杨鹏  刘鹤莹 《中国乳业》2021,(11):105-111

针对规模化奶牛场的粪污处理问题,利用了固液分离技术,对奶牛场内环境治理,尤其是圈舍内的清粪环节进行减量化改进。从技术角度上优化畜舍内的环境质量,从而弥补国内外现有畜禽养殖业中粪污治理模式中的弱项。本文以天津和润畜牧养殖有限责任公司的规模化奶牛场为试验点,对其产生的粪污进行技术化固液分离,分离后的固体粪便经发酵、晾晒后可作为奶牛的卧床垫料而重复利用。污水经稀释匀浆后储存于贮水池,在采用固液分离技术后,每天的产污水量下降了22%,粪污总量约下降21%。通过技术改进,固液分离处理后的粪污中,总悬浮颗粒物占比降低,污水中总氮和总磷含量降低,化学需氧量（COD）和生化需氧量（BOD）降低60%以上,水质得到提升。固液分离技术可为规模化养殖业提供更加便捷高效的处理粪污、降低污染物含量的产业化排污模式,使规模化养殖产品更加安全健康,同时也可以使人民的日常饮食得到安全保障。  相似文献   

梭锥管混浊流体分离装置倾角优化数值模拟研究     

王苗  李琳  谭义海 《节水灌溉》2015,(4)

为了研究锥圈倾角和下锥管倾角对梭锥管内水沙分离效果的影响,在数值模拟计算结果与PIV测试结果一致基础之上,建立了5个不同锥圈倾角和7个不同下锥管倾角的梭锥管模型,采用层流方程和简化的多相流Mixture模型通过数值模拟计算了浑水含沙浓度为5kg/m3时各个梭锥管内部的泥沙浓度和速度场,详细对比分析了不同倾角下梭锥管内的速度场和浓度分布特性。结果表明,在上锥管结构尺寸、锥圈间距不变的条件下,锥圈倾角β与下锥管倾角γ相等且等于45°时,梭锥管水沙分离效果最好,在该角度下锥圈上表面泥沙滑动至排沙通道所需要的时间最短,梭锥管水沙分离效率最高。  相似文献   

Evaluation of effects of olfactory and auditory stimulation on separation anxiety by salivary cortisol measurement in dogs     

Yoon-Joo Shin  Nam-Shik Shin 《Journal of veterinary science (Suw?n-si, Korea)》2016,17(2):153-158

Separation anxiety (SA) is a serious behavioral problem in dogs. In this study, salivary cortisol was studied to determine if the owner''s odor or voice could reduce SA in dogs. Twenty-eight dogs with SA were divided into three groups: group 1 (control), group 2 (with owner''s clothes during the separation period; SP) and group 3 (a recording of the owner''s voice was played during SP). The dog''s saliva was collected after the owner and their dog were in the experimental room for 5 min (PRE). The dog was then separated from the owner for 20 min and saliva collected four times at intervals of 5 min (SP1–4). Finally, the owner was allowed back into the room to calm the dog for 5 min, after which saliva was collected (POST). Evaluation of salivary cortisol concentrations by ELISA revealed that the ratios of SP1 concentration to PRE or POST concentrations were significantly higher in group 1 than in group 2 or 3. Additionally, the concentrations of SP1–PRE and SP1–POST among groups differed significantly. These findings indicate that the owner''s odor or voice may be helpful to managing stress in dogs with SA.  相似文献