全文获取类型
收费全文 | 969篇 |
免费 | 83篇 |
国内免费 | 142篇 |
专业分类
林业 | 152篇 |
农学 | 84篇 |
基础科学 | 148篇 |
173篇 | |
综合类 | 379篇 |
农作物 | 27篇 |
水产渔业 | 60篇 |
畜牧兽医 | 110篇 |
园艺 | 19篇 |
植物保护 | 42篇 |
出版年
2024年 | 17篇 |
2023年 | 41篇 |
2022年 | 53篇 |
2021年 | 54篇 |
2020年 | 47篇 |
2019年 | 55篇 |
2018年 | 33篇 |
2017年 | 59篇 |
2016年 | 71篇 |
2015年 | 47篇 |
2014年 | 47篇 |
2013年 | 66篇 |
2012年 | 77篇 |
2011年 | 58篇 |
2010年 | 64篇 |
2009年 | 50篇 |
2008年 | 46篇 |
2007年 | 55篇 |
2006年 | 36篇 |
2005年 | 36篇 |
2004年 | 25篇 |
2003年 | 31篇 |
2002年 | 26篇 |
2001年 | 22篇 |
2000年 | 13篇 |
1999年 | 13篇 |
1998年 | 5篇 |
1997年 | 8篇 |
1996年 | 4篇 |
1995年 | 2篇 |
1994年 | 7篇 |
1993年 | 6篇 |
1992年 | 3篇 |
1991年 | 2篇 |
1990年 | 7篇 |
1989年 | 3篇 |
1988年 | 3篇 |
1986年 | 1篇 |
1977年 | 1篇 |
排序方式: 共有1194条查询结果,搜索用时 281 毫秒
1.
Abstract – Foraging juvenile fish with relatively high food demands are usually vulnerable to various aquatic and avian predators. To compromise between foraging and antipredator activity, they need exact and reliable information about current predation risk. Among direct predator-induced cues, visual and olfactory signals are considered to be most important. Food intake rates and prey-size selectivity of laboratory-reared, naive young-of-the-year (YOY) perch, Perca fluviatilis , were studied in experiments with Daphnia magna of two size classes: 2.8 and 1.3 mm as prey and northern pike, Esox lucius , as predator. Neither total intake rate nor prey-size selectivity was modified by predator kairomones alone (water from an aquarium with a pike was pumped into the test aquaria) under daylight conditions. Visual presentation of pike reduced total food intake by perch. This effect was significantly more pronounced (synergistic) when visual and olfactory cues were presented simultaneously to foraging perch. Moreover, the combination of cues caused a significant shift in prey-size selection, expressed as a reduced proportion of large prey in the diet. Our observations demonstrate that predator-induced olfactory cues alone are less important modifiers of the feeding behaviour of naive YOY perch than visual cues under daylight conditions. However, pike odour acts as a modulatory stimulus enhancing the effects of visual cues, which trigger an innate response in perch. 相似文献
2.
由专家群体对不同品种、种植密度、树龄、产量进行柑橘园产量等级经验评估,应用回归方法筛选出柑橘园产量等级的统一量化模式,为相关应用研究提供可比参考指标. 相似文献
3.
4.
T. Batey 《Soil Use and Management》2015,31(1):60-66
Since the 1970s a network of underground pipes, up to 1200 mm diameter, has been installed in the UK to transmit crude oil to refineries and gas from onshore terminals for distribution. Chosen routes are subject to significant constraints. Current techniques for pipe installation involve topsoil removal and storage. Trench depth is set to allow a cover of 1200 mm overburden after pipes have been installed. The heavy machinery involved results in severe compaction of exposed subsoil. Subsoil is loosened comprehensively and topsoil replaced. Existing field drains are reconnected, and, if necessary, new drains with gravel backfill installed. Pipe installation usually takes place between April and October. Preventative measures to limit compaction during installation are not a practical option. If subsequent arable crops or grass are poorer or drainage more defective than before disturbance, the loss is assessed and compensation paid. To determine the cause of the loss >60, investigations have been made throughout the UK. Soil physical properties were assessed in an open trench using visual and tactile methods. Consistently, severe compaction in the subsoil was identified as the cause of poor crop growth or drainage; it was not observed in the topsoil. Specific recommendations for remedial action were made based on location, depth and severity of the compaction. These included the installation of additional land drains, increasing the amount of gravel above the drains, or further subsoil loosening orientated to cross gravel backfill. After appropriate remedial action, net compensation for crop losses was frequently small or negligible. 相似文献
5.
6.
骨形态发生蛋白15(bone morphogenetic protein 15,BMP15)基因突变对绵羊排卵率、产仔数以及繁殖力有很大影响,本研究旨在建立快速可视化检测BMP15基因B2突变的技术。将改良的扩增阻滞突变系统(amplification refractory mutation system,ARMS)与核酸染料SYBR Green Ⅰ结合,针对BMP15基因B2突变设计ARMS特异性引物,使引物下游3'端的最后一个碱基为A,并在下游引物3'端的第3位碱基处设计额外的错配,以提高引物的特异性;经ARMS PCR扩增后,向产物中加入SYBR Green Ⅰ,通过肉眼观察PCR管内的颜色变化来检测BMP15基因的B2突变。经测序发现所采集的样本均为野生型,因此,利用重叠延伸PCR构建BMP15基因B2突变型模板,测序结果显示BMP15基因B2突变型模板构建成功。ARMS PCR结果显示,ARMS特异性引物能够只扩增突变型模板,而不会扩增野生型模板,且扩增效果良好。ARMS PCR扩增后加入SYBR Green Ⅰ发现已知野生型模板与阴性对照一致,呈SYBR Green Ⅰ原始颜色橙黄色,而已知突变型模板呈亮绿色,且色差明显,肉眼可辨。用建立的可视化检测BMP15基因B2突变的技术检测50个小尾寒羊基因组DNA (随机向其中20个样本中加入构建的BMP15基因B2突变型模板),将可视化检测的突变型样本编号与混合样本时记录的编号对比,结果表明该技术能够准确检测绵羊BMP15基因的B2突变,且准确率高达100%。将构建的BMP15基因B2突变型的模板进行梯度稀释,对本试验可视化检测体系的灵敏度进行检测,发现ARMS可视化检测技术的灵敏度达到0.006 ng/μL。因此,本研究建立的技术能够可视化地检测绵羊BMP15基因B2突变,且准确率高,有望为高繁殖力绵羊的选育提供技术支持。 相似文献
7.
8.
Filippo Scarpa Silvia Sabattini Giuliano Bettini 《Veterinary and comparative oncology》2016,14(3):245-251
A cytological grading for mast cell tumours (MCTs) would be highly desirable, allowing to select the most appropriate therapeutic intervention prior to surgery. This study evaluates the applicability on fine‐needle aspirations (FNAs) of the novel Kiupel grading system, based on number of mitoses, multinucleated cells, bizarre nuclei and presence of karyomegaly. Fifty consecutive cases with pre‐operative cytological diagnosis were included. In cytological specimens, approximately 1000 cells were evaluated, and the histological grade was assessed on the corresponding resected specimens. On cytology, the above parameters were significantly different between histologically low‐grade and high‐grade tumours (P < 0.001). The cytograding correctly predicted the histological grade in 47 cases (accuracy, 94%; sensitivity, 84.6%; specificity, 97.3%). Two high‐grade MCTs (4%) were not detected on cytology. The cytograding can provide helpful insights to assist clinical decisions in most cases. However, the risk of underestimation in a minority of patients represents a limit to the overall utility of the technique. 相似文献
9.
Eugenia Fezza Joe M. Roberts Toby J. A. Bruce Lael E. Walsh Michael T. Gaffney Tom W. Pope 《Pest management science》2023,79(11):4635-4643
BACKGROUND
Monitoring is an integral component of integrated pest management (IPM) programmes used to inform crop management decisions. Vine weevil, Otiorhynchus sulcatus F. (Coleoptera: Curculionidae), continues to cause economically significant losses in horticultural crops due to an inability to reliably detect the presence of this species before crop damage occurs. To improve vine weevil monitoring we investigated the behavioural responses of adult vine weevils to visual (monitoring tool shade/colour, height and diameter as well as the effect of monitoring tool and plant density) and olfactory (host plant and conspecifics) cues under glasshouse conditions.RESULTS
Monitoring tool shade, height and diameter all influenced monitoring tool efficacy, with individuals exhibiting a preference for black, tall and wide monitoring tools. The total number of individuals recorded in monitoring tools increased with monitoring tool density. By contrast, plant density did not influence the number of individuals recorded in monitoring tools. Yew-baited monitoring tools retained a larger number of individuals compared to unbaited ones. Similarly, more vine weevils were recorded in monitoring tools baited with yew and conspecifics than in unbaited monitoring tools or those baited with only yew. Baiting monitoring tools with conspecifics alone did not enhance the number of vine weevils recorded in monitoring tools.CONCLUSIONS
Our study confirms that visual and olfactory cues influence vine weevil behaviour. This provides information on key factors that influence vine weevil monitoring tool efficacy and can be used to inform the development of a new monitoring tool for this pest. © 2023 The Authors. Pest Management Science published by John Wiley & Sons Ltd on behalf of Society of Chemical Industry. 相似文献10.