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Esdras M. Carbajal M. Carolina Zuleta Luellen Swayzer Brian M. Schwartz Maria Carolina Chavarro A. Carolina Ballen‐Taborda Susana R. Milla‐Lewis 《Plant Breeding》2019,138(6):958-966
St. Augustinegrass is well suited for lawns and commercial landscapes. While many genotypes are cross‐fertile, all cultivars are propagated vegetatively in sod production. To ensure varietal purity, development of sterile triploid hybrids by crossing tetraploid and diploid genotypes has been successfully used in other warm‐season turfgrasses. Applying this model in St. Augustinegrass would be beneficial to sod producers and turf managers who require purity for certification and uniformity for performance, respectively. This study was conducted to develop colchicine‐induced tetraploid lines of St. Augustinegrass. Seeds of cultivar ‘Raleigh’ were treated with four colchicine concentrations at four exposure times. A non‐treated control was included among the treatments. Seedlings that germinated were screened for genome size changes using flow cytometry. Line DSA 13005 and two progeny lines derived through selfing, DSA 16001 and DSA 16016, were corroborated as tetraploids (2n = 4x = 36) through chromosome counts. These lines will be used in future breeding efforts to attempt development of sterile triploid cultivars of St. Augustinegrass. 相似文献
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ABSTRACT Research was conducted to verify the current fertilization recommendations for St. Augustinegrass (Stenotaphrum secundatum Walt. Kuntze) lawns. Currently, a range from 100 to 300 kg nitrogen (N) ha? 1 per growing season is suggested, based on factors such as location in the state, desired maintenance level, soil conditions, etc. Research was conducted at the G.C. Horn Turfgrass Field Laboratory in Gainesville, FL from 2000 to 2002, utilizing medium to high management levels and at a sod farm in Bradenton, FL from 2001 and 2002, under low management conditions. Nitrogen (N) and potassium (K) treatments were arranged in a randomized complete block design with three replications in both locations. In Gainesville, best visual quality was generally obtained with medium (MN) or high nitrogen rates (HN). In Bradenton, MN, and HN rates provided best visual quality responses in 2002 and 2003, but higher rates of N were required than in Gainesville to maintain acceptable quality. This higher N requirement could be due to the longer growing season in Bradenton or to poor turfgrass growth resulting from inadequate pest control. In the absence of biotic or abiotic stresses, the highest rates of N were not necessary to maintain an acceptable quality lawn. 相似文献
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Somaclonal variation has been observed in many plant species and is an alternative way to create variants and expand the germplasm pool. A large scale tissue culture experiment was conducted with St. Augustinegrass, an important turfgrass species for the southern USA, to induce somaclonal variation to enlarge the germplasm pool for breeding efforts. Using an improved protocol, approximately 7900 St. Augustinegrass plants were regenerated from cv. 'Raleigh', and 119 morphological variants were identified. Among the variants, 115 had a semi-dwarf growth habit with shorter and narrower leaves, and shortened internodes and stolons. However, 100 of them showed little vigour, which either grew very slowly or did not survive. The remaining 15 showed reasonable growth vigour and were further investigated in the field. Among them, 13 were semi-dwarf and 2 had longer leaves. In addition, 2 other variants, with variegated (yellow striping) leaves, or significantly thicker stems were also observed and characterized. The altered traits in the variant lines were stable during vegetative propagation and when grown in different environments. 相似文献
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