排序方式： 共有13条查询结果，搜索用时 15 毫秒
1.
Flos Chrysanthemi (the flower of Chrysanthemum morifolium Ramat.) is widely used in China as a food and traditional Chinese medicine for many diseases. Luteolin and apigenin are two main bioactive components in Flos Chrysanthemi, and chrysoeriol and diosmetin are two methylated metabolites of luteolin in vivo by cathechol-O-methyltransferase (COMT). However, there was lack of pharmacokinetic information of chrysoeriol and diosmetin after oral administration of Flos Chrysanthemi extract (FCE). The present study aimed to develop an HPLC-UV method for simultaneous determination of rat plasma concentration of luteolin, apigenin, chrysoeriol and diosmetin and utilize it in pharmacokinetic study of the four compounds after orally giving FCE to rats. The method was successfully validated and applied to the pharmacokinetic study when oral administration of FCE to rats with or without co-giving a COMT inhibitor, entacapone. Chrysoeriol and diosmetin were detected in rat plasma after oral administration of FCE and their concentrations were significantly decreased after co-giving entacapone. Furthermore, AUC of luteolin was significantly increased by entacapone, while that of chrysoeriol was decreased by entacapone, which revealed COMT might play an important role in the disposition of luteolin in rats after dosing of FCE. In conclusion, a sensitive, accurate and reproducible HPLC-UV method for simultaneous determination of luteolin, apigenin, chrysoeriol and diosmetin in rat plasma were developed, pharmacokinetics of chrysoeriol and diosmetin combined with luteolin and apigenin were characterized after oral administration of FCE to rats, which gave us more information on pharmacokinetics and potential pharmacological effects of FCE in vivo. 相似文献
2.
【目的】 研究伊犁马COMT基因第1外显子的多态性及其构建的单倍型,并分析不同基因型、单倍型组合与心率之间的关联程度,为研究COMT基因对马匹气质及生产性能的影响奠定前期基础。【方法】以35匹伊犁马为研究对象,采用酚—氯仿法、DNA测序等技术分析COMT第1外显子基因多态性位点及基因型,通过haploview 4.2 软件构建单倍型,采集马匹心率,并对其与不同基因型、单倍型组合进行关联分析。【结果】伊犁马COMT基因第1外显子中共发现8个突变位点,其中SNP1、SNP2、SNP4、SNP5、SNP7和SNP8为错意突变;除SNP7与其余各突变间均无较强连锁外,SNP1-6、SNP8均存在一定的强连锁关系,SNP1和SNP4完全连锁;构建出9种单倍型,其中H1为优势单倍型;进行不同 SNPs位点及单倍型组合与心率的关联分析发现SNP3和SNP5不同基因型在温和刺激心率上存在显著或极显著性差异(P<0.01或P<0.05),SNP2、SNP5、SNP6、SNP7和SNP8不同基因型在剧烈刺激心率上均有显著或极显著性差异(P<0.01或P<0.05),单倍型组合H1H2在剧烈刺激心率上显著性大于H1H3。【结论】伊犁马COMT第1外显子上存在多态性,且不同突变间存在一定程度的强连锁,不同基因型、单倍型组合个体在心率上存在差异,提示COMT基因可能是对伊犁马气质和生产性能影响的重要功能基因。 相似文献
3.
4.
以2×CTAB法从欧美杨107号嫩叶提取的基因组DNA为试材,建立了木质素合成酶COMT基因的PCR扩增体系。通过对PCR反应的影响因子——模板DNA用量、dNTP浓度、DNA聚合酶量、引物浓度和退火温度进行优化,最终建立了扩增COMT的PCR优化体系为:在20μL的反应体系中,dNTP为2.5 pmol,引物各为5 pmol,模板为5 ng,Red TaqTMDNA聚合酶1.25 U,退火温度为51℃,可以扩增出清晰的扩增带。 相似文献
5.
本研究利用半定量RT-PCR分析方法探索了陆地棉(Gossypium hirsutum L.)木质素合成途径中甲基化酶基因家族COMT和CCoAOMT在不同组织和不同发育时期纤维中的表达情况。结果表明COMT1,COMT2,COMT3和CCoAOMT1,CCoAOMT2在棉花的7个组织器官(根、茎、叶、子叶、花瓣、雄蕊、胚珠)中都有表达,其中COMT1和CCoAOMT1在各组织中的表达趋势类似,COMT2,COMT3和CCoAOMT2表达趋势各异。在5～25d的棉纤维发育过程中,COMT1和COMT3表达稳定,COMT2表达量逐渐增加。而CCoAOMT基因家族的2个基因的表达量变化相对明显,CCoAOMT1在25d出现表达高峰,CCoAOMT2则在10d和15d呈现高表达量。本研究表明甲基化酶基因家族COMT和CCoAOMT在棉花的根,茎等维管组织中具有相对一致的高表达量,而在其他组织和发育纤维中则呈现出明显的组织特异性和纤维发育时期特异性。 相似文献
6.
木质素生物合成关键酶咖啡酸-O-甲基转移酶基因(COMT)的研究进展 总被引：7，自引：0，他引：7
木质素是植物苯丙烷代谢途径的重要产物之一,其生物合成涉及到许多酶的参与。其中咖啡酸-O-甲基转移酶(caffeic acid-O-methyltransferase,COMT)是木质素特异途径中的一个关键酶,可催化咖啡酸、5-羟基松柏醛和5-羟基松柏醇甲基化分别生成阿魏酸、芥子醛和芥子醇,参与S-木质素的合成。本文主要对COMT基因的特征、COMT在木质素生物合成中的作用及COMT基因对植物木质素合成调控的研究现状进行了综述,并对COMT基因的研究方法和在生产中的应用作了展望。 相似文献
7.
以从茶树叶子中提取的总RNA为模板,结合RT-PCR利用RACE技术,得到咖啡酸氧甲基转移酶(caffeic acid o-methyltransferase,COMT)基因全长1 381bp,其开放阅读框为1 092bp,编码393个氨基酸,推测的蛋白分子量约为39.661 9ku,理论等电点为5.66,在GenBank的登录号为HM204933。其核苷酸序列与欧洲白桦、苎麻、中果咖啡和毛果杨的COMT基因相似性分别为80%、78%、77%、77%,氨基酸序列与毛果杨的COMT基因达100%。将该基因重组到表达载体pET-32a(+)中进行原核表达,经IPTG诱导,SDS-PAGE检测结果表明茶树COMT基因能在大肠杆菌BL21(DE3)中表达,电泳检测到一条大约60ku的外源蛋白,与预测的融合蛋白分子量相符。 相似文献
8.
以已经克隆到亚麻木质素合成关键酶咖啡酸-O-甲基转移酶基因(COMT)全长cDNA序列为基础,通过扩增RNAi顺式及反式片段(均为249 bp),先连接到中间载体pBSK,再连接表达载体pCAMBIA-1301-multi,构建成COMT基因的RNAi表达载体,通过农杆菌介导转化亚麻,获得转基因植株,通过GUS染色和PCR检测,表明干扰载体已经转入亚麻中。这为推进亚麻纤维品质改良的分子育种提供了基础。 相似文献
9.
C. Saligaut T. Bailhache G. Salbert B. Breton P. Jego 《Fish physiology and biochemistry》1990,8(3):199-205
Aminergic metabolism was studied in discrete brain regions of the postovulated female rainbow trout using a liquid chromatography
electrochemical detection method. 3 Methoxytyramine (3MT) was the major dopaminergic catabolite, suggesting that catechol-o-methyl
transferase is the main dopamine (DA) catabolic enzyme. Two populations of brain regions were found: one with a high DA content
and low 3MT/DA ratio (hypothalamus and telencephalon), suggesting that these regions could present a high density of DA perikarya;
the other with a high 3MT/DA ratio (pituitary, preoptic area, myelencephalon and optic tectum) suggesting that these regions
could present a high density of DA axonal endings. 5 Hydroxytryptamine (5HT) content differed, but an homogeneous distribution
of monoamine oxidase was found in different brain regions. High 5HT content was found in the hypothalamus and telencephalon;
5HT was however not detectable in the pituitary. 相似文献
10.
L. A. C. Van Asselt R. J. M. Timmers H. J. Th. Goos J. G. D. Lambert P. G. W. J. Van Oordt 《Fish physiology and biochemistry》1989,6(4):207-212
Isolated gonadotrops of the African catfish,Clarias gariepinus, were incubated with dopamine (DA) and/or catecholestrone and the activity of the enzyme catechol-O-methyltransferase (COMT) was determined by measuring the methylated products. From the apparent Km values for DA and catecholestrone of 0.4–1.3 M and 17.9–25.2 M respectively, it was concluded that catecholestrone is a better substrate for the enzyme COMT, compared to DA. Moreover, the methylation of DA is inhibited by comparatively low concentrations of catecholestrone. 相似文献