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排序方式: 共有139条查询结果,搜索用时 15 毫秒
1.
家蚕丝素蛋白对大鼠胆固醇代射的影响 总被引:1,自引:0,他引:1
研究了经酸解后制得的家蚕丝素蛋白氨基酸组成以及对高血脂实验鼠血清和肝脏内胆固醇及转氨酶的活性变化的影响。结果表明:所得的家蚕丝素蛋白粉氨基酸组成丰富,其中甘氨酸、丙氨酸、丝氨酸和酪氨酸含量占全部氨基酸总量的95%;家蚕丝素蛋白能显著地降低血清胆固醇含量和谷草转氨酶及谷丙转氨酶活性,提高动物肝组织中的胆固醇浓度和谷丙转氨酶的活性。 相似文献
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AIM:To explore the effects of oxidized low density lipoprotein (OxLDL) and one of its component— lysophosphatidylcholine (LPC) on cholesterol efflux from mouse macrophage foam cells.METHODS:(1) Cholesterol efflux induced by apoAI from mouse peritoneal macrophage foam cells loaded with OxLDL or acylated LDL(AcLDL) was measured. (2) Cholesterol efflux induced by LPC and apoAI from macrophage foam cells separated from normal or apoE gene deficient (E0) mouse loaded with AcLDL were measured.RESULTS:(1) When the macrophage foam cells were incubated with apoAI, cholesterol efflux from AcLDL-induced macrophage foam cells increased significantly compared to that of OxLDL-induced macrophage foam cells. (2) LPC promoted cholesterol efflux from macrophage foam cells in relation to both dosage and time. When LPC was incubated with E0 mouse macrophage foam cells, the released cholesterol mass was significantly lower than that of normal mouse macrophage foam cells. It was also found that cholesterol efflux induced by apoAI normally occurred in E0 mouse macrophage foam cells.CONCLUSIONS:(1) OxLDL accumulated cholesterol in macrophages and impair cholesterol efflux. (2) LPC induced cholesterol efflux from macrophage foam cells, which may occur via apoE pathway. 相似文献
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Spectrophotometry and Ultracentrifugation for Measurement of Plasma Lipids in Dogs with Diabetes Mellitus
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E.C. Seage K.J. Drobatz R.S. Hess 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2018,32(1):93-98
Background
There are conflicting reports of plasma lipoprotein lipid content in dogs with diabetes mellitus (DM).Objectives
To determine lipoprotein lipid content of plasma of dogs with DM by spectrophotometry and ultracentrifugation; to compare lipoprotein lipid content in diabetic and healthy dogs; and to quantify apolipoprotein B‐100 (ApoB) in dogs with DM.Animals
22 dogs with DM and 9 healthy dogs.Methods
Cross‐sectional study. Triglyceride (TG), total cholesterol (TC), and high‐density lipoprotein cholesterol (HDL‐C) concentrations were measured by spectrophotometry. Very low‐density lipoprotein cholesterol (VLDL‐C) and low‐density lipoprotein cholesterol (LDL‐C) concentrations were calculated after ultracentrifugation. Non‐HDL‐C cholesterol was calculated by subtracting HDL‐C from TC. ApoB was quantified by ELISA. The Mann‐Whitney test was used for comparison of median lipoprotein concentrations, and Spearman's correlation was used to assess associations between ApoB and lipoprotein fractions.Results
All values are reported in mg/dL. Median TG (122), TC (343.5), HDL‐C, (200), VLDL‐C, (27) LDL‐C (68), non‐HDL‐C (114), and ApoB (320) were significantly higher in dogs with DM, compared to healthy dogs (57, 197, 168, 12, 16, 31, and 258, respectively, P‐values 0.0079, <0.001, 0.029, 0.011, <0.001, <0.001, 0.025, respectively). A significant association was found between ApoB and LDL‐C (Spearman's rho = 0.41, P = 0.022) and between ApoB and non‐HDL‐C (Spearman's rho = 0.40, P = 0.027).Conclusions and Clinical Importance
Dyslipidemia of dogs with DM is characterized by pronounced increases in LDL‐C and non‐HDL‐C concentrations, although all lipoprotein fractions are significantly increased. Knowledge of specific lipoprotein fraction alterations in dogs with DM can enhance treatment options for diabetic dyslipidemia in dogs. 相似文献4.
AIM:To investigate the functional changes and histopathological basis of Oddi’s sphincter under hypercholesterolemia. METHODS:Twenty - four New Zealand rabbits were equally divided into be groups ran- domly. Experimental groups Ⅰ and Ⅱ fed with cholesterol - added forage for 4 and 8 weeks respectively before sacri- ficed. Images, functions and histopathological characteristics of Oddi’s sphincters of experimental groups were studied and compared with control group by cholangiography, catheter manometry, and quantitative analysis of nitric oxide synthase. RESULTS: The hasal pressures of proximal low - pressure segment of Oddi’s sphincter of hath experimen- tal groups Ⅰand Ⅱ (20.9±6.1 mmHg, 25 .6±9.1 mmHg, respectively) were higher than those of control group sig- nificantly (11.7±2.8 mmHg, P < 0.01, P < 0.01 ). The hasal pressures of distal high - pressure "valve" segment of both experimental pops (138 .4±45. 5 mmHg and 144. 5 ±40 .4 mmHg, respectively) were significantly higher than those of coned group (69.3±9.8 mmHg, P < 0.01 and P<0.01). The gallbladder volUmes of experimental group Ⅰ and Ⅱ (3.4±1.0 mL and 3.9±1.9 mL) were significantly larger than those of control group (2.0 ±1.9 mL, P < 0.05 and P < 0.01 ). Between groups there were no significant differences in nitric oxide synthase con- tents. Enlargement of the cell volume, wavy distortion of the cell membrane, loosening and disarrangement of myofila- ment, uneven in size and distribution of dense body, angulation of longitudinal axis between dense body and myofila- ment, swelling, congregated at one end of nucleoulus and disappearance of cristae of plasmosomes and concertration of chromosome at nucleolus margin were observed in experimental groups under electron-microscope. CONCLUSION: The diastole function of Oddi’s sphincter could be damaged by hyperecholesterolemia, inducing a functional disorder of Oddi’s sphincter with increased resistance in bile excretion. The result is retertion of bile in the gallbladder. 相似文献
5.
灵芝和菌糠降低鸡蛋胆固醇的试验研究 总被引:1,自引:0,他引:1
选取48周龄海兰褐商品蛋鸡120羽,随机分成3组,试验期为4周,对照组Ⅰ组喂基础日粮,试验Ⅱ组添加2.5g/kg灵芝,试验Ⅲ组添加2.5g/kg菌糠,在一致的背景下,探讨灵芝、菌糠对鸡蛋胆固醇的影响。结果表明:Ⅱ、Ⅲ组可降低蛋鸡血液甘油三酯(TG)、总胆固醇(TC)含量,增高血清高密度脂蛋白胆固醇(HDL-C)含量;3组之间料蛋比差异显著,Ⅱ、Ⅲ组料蛋比分别比对照组低6.4%(P<0.05)、11.0%(P<0.05)。Ⅱ、Ⅲ组平均产蛋率分别比对照组提高5.38%(P<0.05)和3.29%(P<0.05)。试验4周,Ⅱ、Ⅲ组鸡蛋胆固醇含量分别降低15.3%(P<0.05)和10.4%(P<0.05),且两组间差异显著(P<0.05),研究表明灵芝及菌糠均能提高蛋鸡生产性能,降低鸡蛋胆固醇含量。相比之下,灵芝是降低鸡蛋胆固醇较为理想的添加剂。 相似文献
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根据两年来的试验结果我们发现,杜×(长北)仔猪的早期生长速度与血清甘油三酯含量之间,存在着强的负相关性.这预示:甘油三酯作为杜×(长北)仔猪早期生长速度的鉴定指标,是存在着可能性的,试验结果进一步表明,甘油三酯不仅在杜×(长北)仔猪的生长势上有预测价值,而且在三元杂交猪亲本的选择上和瘦肉型商品猪肉质的鉴定上,也具有一定的指导作用。欲改善瘦肉型商品猪的肉质,生产出甘油三酯和胆固醇含量更低、而蛋白质含量更高的三元杂交商品猪,我们认为,杜洛克母猪是首选的亲本之一。 相似文献
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关于人的胆汁中存在液晶已见报道,有人推测液晶的出现与胆结石有关。我们用正交的偏振光显微镜观察了猪、鸡的胆汁,并将观察结果和人的胆红素结石伴生胆汁及肌同醇结石伴生胆汁的观察结果进行比较,发现了他们的异同之处.从而使我们对胆汁液晶的产生及其性质,以及胆汁液晶和胆结石之间的关系有了进一步的了解. 相似文献