柑桔体细胞杂种扦插繁殖及其有关性状研究     

邓秀新  马湘涛  孙中海  官守国 《果树学报》1997,(2)

对３个由原生质体融合产生的柑桔体细胞杂种繁殖特性的研究表明，金柑与甜橙属间体细胞杂种扦插生根成活率较低。甜橙＋宜昌橙以及甜橙＋粗柠檬种间体细胞杂种扦插萌发和生根能力与权及粗柠檬没有显著差异；插条发根平均为２．０条和１．８条，一年生植株根系总长分别为１２２．２ｃｍ和８０．７ｃｍ，与积和粗柠檬均无显著差异；粗柠檬＋甜橙杂种的须根数为３９条，株－１，与权（５４．５条·株－１）差异显著，而宜昌橙＋甜橙种间杂种（４７．７条·株－１）与积差异不显著；体细胞杂种的抗寒性有介于双亲之间的趋势  相似文献   

盐分对碱茅种子发芽影响的机制   总被引：15，自引：0，他引：15  

阎顺国 Bake.  DA 《草地学报》1994,2(2):12-19

耐碱茅种子在盐（NaCl与Na2SO4）及干旱（PEG－6000模拟）胁迫下发芽及其机制的比较研究表明，种子发芽率随溶液渗透势的降低而下降，在等渗条件下三种溶液中的发芽率次序为Na2SO4＞NaCl＞PEG。NaCl与Na2SO4主要通过影响种子吸水量及对膜的毒性影响发芽，根据相关及通径分析结果，其机制为：（1）溶液渗透势及Na+浓度决定的种子吸水对发芽的直接正效应（通径系数0．73）；（2）溶液Na+浓度引起的膜毒性对发芽的直接负效应（－0．51）；（3）种子从溶液中吸收Na使种子吸水增加对发芽的间接正效应（0．30）及使膜毒性增大对发芽的间接负效应（－0．24）。  相似文献   

稀释液渗透压对绵羊精液冷冻效果的影响   总被引：3，自引：0，他引：3  

曾培坚  王立铭 《中国畜牧杂志》1992,28(4):17-19

本试验对绵羊冻精采用两次稀释时,从调整Ⅱ液中葡萄糖含量和降低甘油浓度两方面,改变稀释液的渗透压,测定冻精效果。先增减糖的比例,配制9-2(对照), 84-1和84-2等试验组,其稀释Ⅱ液渗透压1191,1026、1499mmol/kg,获冻精情期产羔母羊率61.69, 63.31, 54.55(P<0.05)。随后, 以84-1为对照,降低甘油浓度,设3个试验组,即87-2,88-1和88-1a,渗透压相应为747,456,463mmol/kg,冻精情期受胎率1987年84-1,87-2为65.37,67.37;1988年87-2,88-1,88-1a为63.16,62.50,46.55(P<0.05)。试验说明,绵羊冻精采取2次稀释法,第Ⅱ液以4％甘油,渗透压为747mmol/kg时,冻精效果最佳。  相似文献   

Performance and endocrine responses of group housed weaner pigs exposed to the air quality of a commercial environment     

C. Lee  L.R. Giles  J.L. Downing  A.C. Kirby 《Livestock Production Science》2005,93(3):255-262

The growth performance and endocrine responses of male weaner pigs (3 to 8 weeks of age) was evaluated in two different environments (clean and dirty) and housing (single or groups of 10 pigs/pen) conditions. The dirty environment contained significantly elevated ammonia, carbon dioxide and dust levels compared with the clean environment. Pigs grew faster and consumed more feed in the clean environment and this was associated with reduced plasma cortisol concentrations compared with pigs in the dirty environment. Pigs housed in groups in the dirty environment had increased β-endorphin and decreased IGF-I concentrations compared to group housed pigs in the clean environment. Feed conversion efficiency did not differ due to environment or group housing. Plasma concentration of cortisol, β-endorphin, IGF-I and IGF-II did not differ between single and group housed pigs. Activity of the hypothalamic-pituitary-adrenal (HPA) axis was greater in response to environmental conditions than group housing, and this was associated with reduced growth in weaner pigs.  相似文献   

Stress parameters and immune response of layers under different cage floor and density conditions     

《Livestock Production Science》2005,95(3):255-263

The aim of the experiment was to investigate the effects of cage floor and cage density on stress parameters of laying hens. A total of 162 brown laying hens (Hyline Brown), aged 34 weeks, were used in the experiment. Compact-type battery cages, with three floors, were used. Hens were allocated as one, three or five hens in each of 18 cages to obtain three different cage density groups of 1968, 656 and 393.8 cm² floor area per hen, respectively. The same number of cages with different cage density were allocated to three different battery floors (first floor=top, second=middle, third=bottom) systematically. Values for body weight, mortality rate, egg weight, egg production, egg quality characteristics, egg yolk cholesterol content, the levels of blood plasma corticosterone, serum glucose, total cholesterol and triglycerides, the ratio of heterophils to lymphocytes (H-L ratio), antibody titers, claw length score, foot health score, plumage score and throat skin injuries were taken as indicators of stress. The values for egg weight (P<0.01) at the first floor were greater than the other floor levels. The group with five hens per cage had significantly lower mean estimates (P<0.01) than other groups with respects to body weight (P<0.001), egg production (P<0.001), egg weight (P<0.001) and plumage score (P<0.01), while significantly higher mean estimates for egg albumen index (P<0.01), Haugh unit (P<0.01), serum glucose (P<0.001), and H-L ratio (P<0.001). Serum cholesterol was higher in cages with one hen than that with five hens, whereas plasma corticosterone was lower. Antibody titers in cages with one hen was similar to that with three or five hens; however, those with three hens had higher titers than those with five hens. Values for egg breaking strength, yolk index, egg cholesterol content, and foot health score were not affected by cage density or floor. The results suggest that the allocation of three hens per cage had no measurable effect on health and welfare.  相似文献   

多胺在果实生长发育中的作用研究进展   总被引：1，自引：0，他引：1  

李建勇  卢钢  任彦 《果树学报》2005,22(3):256-260

多胺是广泛存在于植物体内的具有生物学活性的低分子量脂肪族含氮碱。它在植物的胚胎发育、花和果实的发育等生长发育过程具有重要的调控作用,同时在延缓植物衰老和抗逆境等方面也具有重要意义。近几年的研究表明多胺代谢同植物开花坐果、果实发育甚至果实品质特性密切相关。概述了多胺对果实坐果,膨大与衰老等果实发育生理过程的影响,并对多胺在果实逆境发育中的作用进行了探讨,对多胺在果实发育过程中可能的调控作用机理以及外源多胺在植物开花坐果上的应用潜力进行了讨论,并展望了多胺作用分子机理的研究前景。  相似文献   

The basic mechanism of increased microvascular permeability     

ZHAO Ke-sen  HUANG Qiao-bing 《园艺学报》2003,19(4):549-553

The increased microvascular permeability appears mainly in venule during inflammation, shock, and burns. Endothelial cells play an important role in venule permeability enhancement. There are two kinds of pathway for macromolecule extravasation. One is paracellular pathway and another is transcellular pathway, which are related to the formation of endothelial gap or transcellular openings seperately. The alteration of intercellular related protein, such as occludin, claudin, zona occludens (ZO), junctional adhesion molecule (JAM), VE cadherin, catenin, integrin, etc, and the alteration of endothelial cytoskeleton, such as rearrangement of actin filament, formation of stress fiber and focal adhesion, etc, involve in the pathogenesis of increased microvascular permeability.  相似文献   

Effect of lansoprazole on gastric ulceration in rats     

XU Hua  YU Li-ping  WEN Jie  ZHAO Wen  HUANG Ya-dong  LI Xiao-kun 《园艺学报》2002,18(6):698-700

AIM:To investigate the effect of lansoprazole on gastric ulceration in rats. METHODS:Using the gastric ulcer model induced by hemorrhagic shock, restraint water-immersion stress and pylorus-ligature, the protective effect of lansoprazole (iv) on gastric ulceration was observed. RESULTS:Pretreatment with lansoprazole (7.5-60 mg/kg) significantly inhibited the formation of gastric ulcer in the three models in a dose-dependent manner. The autiulcer efficacy of lansoprazole was similar to that of omeprazole in the equal dose, but stronger than that of omeprazole for ulcer induced by water-immersion stress.CONCLUSION:The intravenously administered lansoprazole inhibited formation of experimental gastric ulcer in rats.  相似文献   

Effect of Jiawei Sini Decoction on glucocorticoid receptor in thymocytes of chronic psychological stress rats     

YAN Can  SHI Ya-fei  LI Yan  WU Zhi-wei  WU Li-li  PAN Yi 《园艺学报》2002,18(12):1494-1496

AIM: To observe the effect of Jiawei Sini Decoction (JWSND) on glucocorticoid receptor (GCR) in thymocytes of chronic psychological stress rats. METHODS: The rats were randomly divided into 4 groups, control group (C), model group (M), group treated by JWSND C₁, group treated by ginsenosides C₂. The number of thymocyte GCR sites and the GCR nuclear thanslocation rate were detected by radioimmunoassay. RESULTS: Compared with the control group, in the model group, the thymocyte weight index lowered significantly ( P<0.05 ), and the GCR nuclear thanslocation rate was increased significantly ( P<0.01 ), but the number of thymocyte GCR sites was unchanged. Compared with the model group, thymus gland weight indexes of C₁ and C₂ were increased significantly ( P<0.05 ), while the GCR nuclear thanslocation rate lowered significantly ( P<0.01 ). Moreover, no significant difference was found in all indexes between C₁ and control group. CONCLUSION: The inhibitory effect of glucocorticoid on the thymus could be significantly reversed by JWSND via suppressing the thanslocation of GCR from cytoplasm to nucleus in chronic psychological stress rats.  相似文献   

Osmolarity,cell volume and proliferation in nasopharyngeal carcinoma cells     

WANG Li-wei  CHEN Li-xin  MAO Jian-wen  ZHU Lin-yan  NIE Si-huai  ZHONG Ping  SUN Xue-rong  CAI Bo  LI Pan 《园艺学报》2004,20(8):1349-1352

AIM: To investigate the relationship between osmolarity, cell volume and cell proliferation in nasopharyngeal carcinoma cells. METHODS: MTT method was applied to detect the proliferation ability of the poorly-differentiated nasopharyngeal carcinoma cell (CNE-2Z) under various osmolarity conditions. The flow cytometry was used to analyse cell cycle distribution. Cell volume was obtained by the image analysis of living cells and cell viability was determined by the trypan blue assay. RESULTS: Cultivation of cells under the hypertonic conditions of 370 and 440 mOsmol/L increased cell volume by 8.7% and 27.8% and facilitated cell proliferation by 22.2% and 33.9%, respectively. However, hypotonic incubation of cells with osmolarity of 160 and 230 mOsmol/L decreased cell volume by 12.8% and 4.1% and inhibited cell proliferation by 34.0% and 15.6%, respectively. Cell volume was positively correlated with cell proliferation rate. Long-term cultivation of cells under anisotonic conditions did not significantly alter cell cycle distribution, but hypotonic cultivation decreased cell viability. CONCLUSION: Proliferation of nasopharyngeal carcinoma cells was closely correlated with the osmolarity of culture medium and cell volume. Hypotonic cultivation may inhibit cell proliferation by decreasing cell volume to facilitate cell death mechanisms.  相似文献