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Duraisamy Saravanakumar Annalisa Ciavorella Davide Spadaro Angelo Garibaldi M. Lodovica Gullino 《Postharvest Biology and Technology》2008,49(1):121-128
A new strain of Metschnikowia pulcherrima (MACH1) was studied for its efficacy as a biocontrol agent against Botrytis cinerea, Penicillium expansum and Alternaria alternata on apples stored for 8 months at 1 °C. The results of two semi-commercial trials demonstrated the efficacy of the biocontrol strain MACH1. In order to understand the mechanism of action involved, the yeast strain was investigated for its competitive ability for iron against postharvest pathogens of apple. M. pulcherrima strain MACH1 was cultivated on PDA with different concentrations of iron (supplemented as FeCl3) against A. alternata and B. cinerea. The yeast strain MACH1 produced a wider pigmented inhibition zone against both pathogens in low iron amendments while less inhibition was measured with increased iron concentrations. At the coloured inhibition zone, B. cinerea and A. alternata conidia did not germinate and mycelial degeneration was observed. In addition, a high reduction in infection by both pathogens was recorded in apples treated with M. pulcherrima strain MACH1 supplemented with low iron amendments compared to higher iron concentrations. The same experiments were carried out in vivo and in vitro against P. expansum. M. pulcherrima strain MACH1 amended with low iron concentration (5 μg mL−1 FeCl3), showing modest lesion diameter reduction and no effect on P. expansum under increased iron and without iron amendments. This study illustrated that iron depletion by the yeast strain MACH1 under low iron conditions could reduce the growth of some postharvest pathogens in vitro and in vivo. Although, iron depletion seems to be a primary mode of action against the postharvest pathogens studied, other mechanisms of action cannot be excluded in the biocontrol employed by M. pulcherrima strain MACH1. 相似文献
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