全文获取类型
收费全文 | 5487篇 |
免费 | 242篇 |
国内免费 | 332篇 |
专业分类
林业 | 920篇 |
农学 | 357篇 |
基础科学 | 108篇 |
265篇 | |
综合类 | 2578篇 |
农作物 | 280篇 |
水产渔业 | 313篇 |
畜牧兽医 | 688篇 |
园艺 | 407篇 |
植物保护 | 145篇 |
出版年
2024年 | 7篇 |
2023年 | 52篇 |
2022年 | 78篇 |
2021年 | 92篇 |
2020年 | 113篇 |
2019年 | 142篇 |
2018年 | 81篇 |
2017年 | 171篇 |
2016年 | 199篇 |
2015年 | 157篇 |
2014年 | 244篇 |
2013年 | 251篇 |
2012年 | 399篇 |
2011年 | 456篇 |
2010年 | 386篇 |
2009年 | 379篇 |
2008年 | 339篇 |
2007年 | 357篇 |
2006年 | 295篇 |
2005年 | 263篇 |
2004年 | 214篇 |
2003年 | 170篇 |
2002年 | 167篇 |
2001年 | 135篇 |
2000年 | 119篇 |
1999年 | 101篇 |
1998年 | 107篇 |
1997年 | 75篇 |
1996年 | 70篇 |
1995年 | 67篇 |
1994年 | 72篇 |
1993年 | 53篇 |
1992年 | 43篇 |
1991年 | 30篇 |
1990年 | 31篇 |
1989年 | 52篇 |
1988年 | 30篇 |
1987年 | 19篇 |
1986年 | 7篇 |
1985年 | 7篇 |
1984年 | 8篇 |
1983年 | 7篇 |
1982年 | 2篇 |
1981年 | 4篇 |
1980年 | 4篇 |
1979年 | 6篇 |
排序方式: 共有6061条查询结果,搜索用时 15 毫秒
1.
为明确北京地区南瓜病毒病种类及其主要侵染病原,2016~2017年在北京周边采集疑似感染病毒的南瓜病样84份,并根据南瓜上的6种病毒特异性引物对其进行反转录PCR(RT-PCR)检测。结果表明:共有79份南瓜病样检测显示阳性,其中黄瓜花叶病毒(Cucumber mosaic virus,CMV)的检出率最高,为52.38%;其次是小西葫芦黄花叶病毒(Zucchini yellow mosaic virus,ZYMV)和西瓜花叶病毒(Watermelon mosaic virus,WMV),检出率分别为44.01%、14.29%,其他病毒暂未检出。此外,16.67%的样品受2种病毒复合侵染,CMV和ZYMV复合侵染占7.14%,ZYMV和WMV复合侵染占9.52%。北京地区南瓜上优势病毒种类为CMV,且存在病毒复合侵染现象。 相似文献
2.
凉山半细毛羊新品种的育成,是采用表型选择和基因型选择相结合产生的结果。在初生期、断奶期、育成期和成年期采取表型鉴定、逐步选择淘汰的制度。根据羔羊断奶期性状与育成期性状之间的显著相关性对羔羊单性状进行早期选择。应用同期发情与后裔测验相结合的方法,对种公羊基因型值用BLUP法进行选择。同时,加强育种工作的组织领导,组建独具特色的三级繁育体系,改善育种羊群的饲养管理条件,圆满完成了国家下达的半细毛羊新品种选育的任务,填补了国内粗档半细毛羊品种的空白。 相似文献
3.
4.
5.
Variations in parameters of liver function and plasma progesterone related to underfeeding and ketosis in a dairy herd 总被引:2,自引:0,他引:2
Twenty-eight Norwegian Red Cattle dairy cows were fed silage ad libitum and restricted amounts of concentrates. Blood samples were collected before morning feeding, once or twice weekly, from 2 weeks before to 12 weeks after calving. Parameters of liver function, carbohydrate status and fertility were recorded in order to assess their interrelationships. Eight cows were treated for clinical ketosis. Four of these had to be treated 2 or 3 times. Aspartate aminotransferase and bilirubin showed the highest within-animal coefficients of correlation with acetoacetate. Analysis of variance revealed a significant effect of carbohydrate status (indicated by plasma acetoacetate levels) on the levels of aspartate aminotransferase, glutamate dehydrogenase and sorbitol dehydrogenase, though only a small part of the total variation was explained by this factor. The estimated volume density of liver fat in the 4th week of lactation averaged 6.0 +/- 6.4% (+/- SD) ranging from 0.1-25.1%. Liver fat content at this stage of lactation was not significantly correlated with other indicators of liver function or carbohydrate status. Cows treated for clinical ketosis had significantly lower plasma progesterone values at the time of first ketosis treatment than untreated multiparous cows. The frequency of high progesterone values (greater than 3 ng/ml) being significantly lower in treated than in untreated cows during the period from 3-5 weeks post partum, though not at later stages. In conclusion, the results revealed a significant relationship between carbohydrate status and liver function, and also between clinical ketosis and luteal function. 相似文献
6.
用分离到的空肠弯曲菌制备鸡空肠弯曲菌甲醛灭活疫苗 ,并进行了该病的免疫保护实验。制备 2× 10 9个 / m L浓度的细菌悬液 ,4m L / L浓度的甲醛溶液作用 12 h灭活后制成空肠弯曲菌灭活苗 ,免疫 2周龄的 SPF雏鸡 ,14 d后 ,进行攻击实验 ,观察其临床症状 ;并分别于感染后 7d和 14 d捕杀 ,观察病理学变化及进行细菌分离实验以揭示其保护率。结果表明 ,鸡空肠弯曲菌灭活苗的保护率可达 10 0 % ,说明该苗经口免疫后安全可靠 ,无毒副作用 相似文献
7.
8.
DENG Zhi-rui ZHANG Rong-xian 《水稻科学》2004,11(1)
Two japonica rice varieties, Wuyujing 3 and 97-7, were used to study the changes in contents of soluble protein, free amino acids and endopeptidase activity during photosynthetic declination. The content of soluble protein in flag leaf of cv.Wuyujing 3 was higher than that of cv. 97-7, but decreased rapidly in Wuyujing 3. Free amino acids in flag leaf and the thirteenth leaf of Wuyujing 3 started to increase 10 days before the turning point of photosynthetic declination (TPPD), while it occurred just 1-2 days before TPPD in the flag leaf and the thirteenth leaf of 97-7. During reversible phase of photosynthetic declination,endopeptidase activity remained at a low level and increased slightly only in the later part of this phase. Then it rose up rapidly at irreversible decline phase and reached a very high level. For Wuyujing 3, the change in endopeptidase activity in the thirteenth leaf was parallel to that in flag leaf. However, for 97-7, the rapid increase of endopeptidase activity in the thirteenth leaf started later than that of flag leaf. The results implied that the rate of protein breakdown and conversion to transportable nitrogen in leaves of 97-7 was slower than that in leaves of Wuyujing 3 during photosynthetic declination and it led to relatively lower seed setting rate and fully filling grains rate in 97-7. This may be one of the important reasons why 97-7 could not bring the high yield potentiality into play and the findings may be taken into consideration while breeding for high potential varieties in future. 相似文献
9.
10.
选用1日龄肉用雏鸡300只,随机分为4组:I组为健康对照;Ⅱ、Ⅲ组喂100ppm锗─132(Ge-132);Ⅲ、Ⅳ组人工感染马立克氏病病毒(MDV)。经60d临床观察并每隔10d用电子自旋共振波谱(ESR)法测定胸腺、法氏囊及脾组织中自由基含量,同时检测全血GSH-Px、血清SOD活性及血清丙二醛含量。结果,健康雏鸡全血GSH-px活性均值为7.9387±1.2378U/mL血清SOD活性为80.4643±1.0987nU/mL血清丙二醛含量为2.9978±01760nmol/mL;健康雏鸡胸腺、法氏囊及脾组织中自由基平均含量分别为:0.27±0.04、0.34±0.08、0.28±0.06相对浓度(×1010spins/g湿重)。通过与健康对照组比较,人工感染MDV雏鸡全血GSH-px和血清SOD活性明显降低,而上述各组织中自由基和血清丙二醛含量则显著增多。Ⅲ、Ⅳ两组对比结果表明:Ⅲ组的全血GSH-px和血清SOD活性高于Ⅳ组,组织中自由基和血清丙二醛含量却低于Ⅳ组,从而证实Ge-132能增强人工感染MDV雏鸡的抗氧化功能。 相似文献