芦笋茎枯病病原菌及其生物学研究   总被引：2，自引：1，他引：2  

苗华民  侯绪友  盂凡明  徐玉恒 《植物保护学报》1991,18(1):87-90

芦笋茎枯病是一种分布广、为害重的毁灭性病害。研究证实,临沂市芦笋茎枯病病原为Phoma asparagi Sacc.。该菌分生孢子发芽和菌丝生长适温为23—26℃,致死温度分别为50℃10分钟和60℃10分钟,发芽和生长最适pH为7。分生孢子生长在分生孢子器内,单个分生孢子常温下可活10天,菌块内可活25天,孢子器内则长达240天。分生孢子在蒸馏水中不发芽,加糖液发芽率略有提高,在文竹和芦笋茎煮液中发芽率最高;条件适宜时2小时产生芽突,5小时发芽率达98.21%。  相似文献   

矮生长豇豆的生育与产量形成   总被引：7，自引：1，他引：6  

关佩聪  刘厚诚 《中国蔬菜》1998,1(6):5-0

对春播和秋播矮生长豇豆的生长动态,叶面积形成与效能,结荚与豆荚产量形成特性等进行研究,结果表明,春播矮生长豇豆植株和各个器官的生长动态均呈Ｓ形曲线变化。秋播矮生长豇豆虽然无抽蔓期,但其生长动态与春播基本相同,而且植株生长和叶面积都较大,单株结荚稍多,但产量无明显差异。净同化率（ＮＡＲ）在营养生长过程较高,生殖生长过程较低。本文还讨论了矮生与蔓生长豇豆生育与产量形成的差异。  相似文献   

缺磷胁迫对长豇豆幼苗脱落酸含量的影响   总被引：1，自引：0，他引：1  

刘厚诚  邝炎华  陈日远 《植物营养与肥料学报》2004,10(6):629-632

研究耐缺磷程度不同的长豇豆品种在缺磷胁迫下脱落酸(ABA)含量的变化。结果表明缺磷胁迫下长豇豆幼苗根系ABA含量都增高,缺磷前期下降后升至1个高峰后再降低,高峰出现的迟早与品种耐缺磷性有密切关系,不耐缺磷的二芦白比耐缺磷的芦花白出现早,平均升幅香港青二芦白芦花白。缺磷胁迫下长豇豆幼苗老茎叶和最嫩完全展开叶ABA含量升高,平均升幅香港青二芦白芦花白,但缺磷后期ABA含量二芦白香港青芦花白;缺磷胁迫下幼苗嫩茎叶ABA含量各品种都升高,升幅芦花白二芦白香港青。缺磷胁迫下长豇豆幼苗茎叶ABA含量的增加可能促进磷和水分的运输、减轻膜质过氧化,是对缺磷胁迫的适应机制之一。  相似文献   

一种芦笋茎枯病防治喷头的试验研究     

高星星  姚世东  陈凯斌  田满洲  曾凡琮  刘海 《天津农业科学》2014,(9):62-64

为促进本地区芦笋种植业规模化发展,提高芦笋种植产量,减少芦笋茎枯病防治用工成本,提出一种喷雾器喷头。采用该喷头进行施药,可以代替人工涂抹施药方式。对该喷头进行了试验研究,研究结果显示,3喷头喷雾器能明显降低芦笋茎枯病防治用工投入,且具有一定的茎枯病防治能力,在一定程度上可以替代人工涂茎作业,为芦笋规模化种植提供了基础。  相似文献   

冬虫夏草水提取物与速溶芦笋粉抗焦虑活性研究     

汤建亚  胡玉婷  何晨梦  孙进  马淑凤  黄云祥 《安徽农业科学》2014,(14):4224-4225,4228

[目的]研究冬虫夏草水提取物与速溶芦笋粉的抗焦虑作用.[方法]取健康雄性SD大鼠70只,按体重随机分为7组,模型对照组,冬虫夏草低剂量组（8.4 mg/kg WECS-1）,冬虫夏草高剂量组（16.8 mg/kg WECS-h）,冬虫夏草水提取物低剂量＋速溶芦笋粉（8.4mg/kg WECS-1 ＋400 mg/kg IAP）,冬虫夏草高剂量＋速溶芦笋粉（16.8 mg/kg WECS-h ＋400 mg/kg IAP）,速溶芦笋粉（400 mg/kgIAP）,空白对照组.采用慢性情绪应激模型,从行为学观察和内分泌水平角度研究速溶芦笋粉的抗焦虑作用.[结果]行为学观察表明,与模型对照组比较,在此焦虑模型上WECS-h ＋IAP剂量组的攻击行为显著降低（P＜0.05）,WECS-1＋ IAP剂量组的探究行为和修饰行为显著降低（P＜0.05）,其他各个处理组与模型对照组比较统计结果均没有显著的差异（P＞0.05）.内分泌水平检测结果表明,与模型对照组比较,在此焦虑模型上冬虫夏草水提取物与速溶芦笋粉（WECS-h ＋IAP）剂量组能显著降低血清种皮质醇（CORT）、肾上腺素（E）和去甲肾上腺素（NA）浓度（P＜0.05）,并能显著升高血清中5-羟色氨酸（5-HT）和多巴胺（DA）水平（P＜0.05）.[结论]冬虫夏草水提取物与速溶芦笋粉联用具有增强抗焦虑作用.  相似文献   

RAPD and SCAR markers linked to the sex expression locus M in asparagus   总被引：13，自引：0，他引：13  

Chunxiao Jiang  Kenneth C. Sink 《Euphytica》1997,94(3):329-333

Bulk segregant analysis (BSA), random amplified polymorphic DNA (RAPD) and sequence characterized amplified region (SCAR) methods were used to map molecular markers to the sex locus M of asparagus. Two parents, A19 (male, Mm) and MW25 (female, mm), and 63 progeny were used for the study. Two DNA bulks, one male and one female, were made by pooling equal amounts of DNA from 10 randomly selected progeny of each sex type. A total of 760 arbitrary decamer oligonucleotide primers were used for RAPD analysis. Primer OPC15 produced two RAPD markers, OPC15-98 and OPC15-30, both of which were linked to the M locus at a distance of 1.6 cM. Subsequently, amplified RAPD fragment OPC15-98 was cloned and sequenced. The sequence was then used to design flanking 24-mer oligonucleotide SCAR primers SCC15-1 and SCC15-2. Both of these SCAR primers amplified a single 980 bp fragment; the same size as the cloned RAPD fragment. However, the SCAR marker was dominant as was the original OPC15-98 band from which it was derived. These RAPD and SCAR markers could be used for scoring male and female progeny in the mapping population, but were not found to be applicable to other asparagus germplasm studied. This revised version was published online in July 2006 with corrections to the Cover Date.  相似文献   

Chloroplast DNA diversity in eggplant (Solanum melongena) and its related species S. incanum and S. marginatum     

Yoshiteru Sakata  Richard N. Lester 《Euphytica》1994,79(1-2):1-4

Summary The potential of isozymes for distinguishing asparagus varieties was carried out by a survey on 21 varieties using 10 enzyme systems: GOT, SkDH, DIA, PGM, MDH, IDH, PGD, ACP, PGI, MR and ADH. Only 3 enzymes, SkDH, GOT and PGM, showed useful polymorphisms. The varieties were found heterogeneous according to their genetic structure: open pollinated varieties were more heterogeneous than clonal hybrids; the F1 hybrid and the vitroclones were homogeneous. As expected from the narrow genetic basis of the varieties, only a few alleles per isozyme locus were present. Moreover, for each enzyme, one allele or type was predominant so that the discriminating power of the method was low. However some of the varieties could be identified and different applications of the results are presented.Abbreviations D.U.S.- Distinction-Uniformity-Stability - ACO- aconitase - ACP- acid phosphatase - ADH- alcohol dehydrogenase - CAT- catalase - DIA- diaphorase - END- endopeptidase - GOT- glutamate oxaloacetate transaminase - IDH- isocitrate dehydrogenase - MDH- malate dehydrogenase - MR- menadione reductase - PGI- phosphoglucoisomerase - PGM- phosphoglucomutase - PGD- phosphoglucose dehydrogenase - POX- peroxidase - SkDH- shikimate dehydrogenase  相似文献   

酶法提取芦笋皮中高活性膳食纤维的研究   总被引：7，自引：3，他引：7  

冯翠萍  庞候英  常明昌  张信号 《农业工程学报》2004,20(3):188-191

以芦笋皮为原料，采用酶法水解制备高活性膳食纤维。试验结果表明：用0.54%的脂肪酶(100 U/mg)、0.80%的淀粉酶(25 U/mg)和0.80%的糖化酶(20 U/mg)、1.20%的蛋白酶(100 U/mg)可提取出高活性的膳食纤维。所得膳食纤维纯度高，总膳食纤维含量达83.08%，其中水溶性膳食纤维17.02%，不溶性膳食纤维66.06%。而且生理活性好，持水性和溶胀性分别为886%和4.5 mL/g。  相似文献   

蔬菜新害螨—吸腐薄口螨 Histiostoma sapromyzarum(Dufour)记述     

李云瑞 《西南大学学报(自然科学版)》1987,(1)

本文记述了为害石刁柏的一种新害螨吸腐薄口螨的形态和为害习性。  相似文献   

固态发酵芦笋老茎生产高蛋白反刍动物饲料的研究     

王琳琳  韩建荣  李欣欣  李艳婷 《饲料工业》2011,32(21)

通过单菌种固态发酵、复合菌种固态发酵以及不同配比的复合菌种固态发酵试验,对能够将芦笋老茎转化为反刍动物饲料的微生物菌种进行了筛选。结果表明,由青霉(Penicillium sp.)F-5、暗孢毛壳(Chaetomium atrosporum)F-21、曲霉(Aspergillus sp.)F-25菌株组成的配比为2:2:3的复合菌种是效果最好的菌种组合。芦笋老茎生料培养基经该复合菌种5 d固态发酵后,发酵产物中可溶性蛋白含量达到4.80 mg/g,与对照组相比增加了39.94%;粗蛋白含量达到11.18%,与对照组相比增加了38.54%;纤维素由22.74%降低到22.32%,降低了1.85%;木质素由12.90%降低到10.53%,降低了18.37%。这些结果证明芦笋老茎经微生物发酵后能转化为良好的高蛋白反刍动物饲料。  相似文献