影响芥蓝×甘蓝RAPD标记稳定性的外部条件优化研究     

陈书霞  房玉林  王晓武 《西北农林科技大学学报(自然科学版)》2005,33(8):103-106

通过对影响RAPD标记稳定性的引物浓度梯度、退火温度梯度、dNTP浓度梯度、Taq酶浓度梯度等外部条件的优化研究,确定了RAPD反应适宜的引物浓度为0.1~0.2pmol/μL,退火温度为48~52℃,dNTP浓度为0.3~0.15mmol/L及Taq酶浓度为0.3U/μL,提高了该反应体系中RAPD的稳定性。  相似文献   

基于CEQ遗传分析仪的AFLP条件的优化试验   总被引：1，自引：0，他引：1  

宋春艳  楚鹰  陈明杰  谭琦  潘迎捷 《食用菌》2006,28(3):14-15

对AFLP选择性扩增反应的dNTP和MgCl2浓度进行了优化试验，产物检测与分析在CEQ遗传分析仪上进行。结果表明：在0．2mMdNTP和2．0mMMgCl2的条件下获得的扩增结果最佳，即AFLP条带最多，毛细管电泳的信号强度最高。  相似文献   

Interspecific hybridization in Lonicera caerulea and Lonicera gracilipes: The occurrence of green/albino plants by reciprocal crossing     

Tomomi Miyashita  Yoichiro Hoshino 《Scientia Horticulturae》2010

Lonicera caerulea L. var. emphyllocalyx (Maxim.) Nakai is a berry crop cultivated in cold regions. So far, commercial cultivars have been mainly introduced from selection of wild plants. Therefore, fruit traits and other agricultural characteristics have been limited. In this study, interspecific crosses between L. caerulea var. emphyllocalyx and Lonicera gracilipes var. glabra Miquel were examined to increase genetic variability of L. caerulea var. emphyllocalyx. Seedlings were obtained from reciprocal crosses between L. caerulea var. emphyllocalyx and L. gracilipes var. glabra. The hybrid nature of seedlings was confirmed with random amplified polymorphic DNA analysis. Viable green plants were obtained efficiently from L. gracilipes var. glabra × L. caerulea var. emphyllocalyx. In contrast, all plants produced from L. caerulea var. emphyllocalyx × L. gracilipes var. glabra were albino. These albino plants were very weak and only survived in culture condition. The chlorophyll deficiency was unilaterally observed, suggesting the occurrence of nuclear–cytoplasmic incompatibility. Viable F₁ hybrids obtained from L. gracilipes var. glabra × L. caerulea var. emphyllocalyx are amphidiploid (2n = 4x = 36) as showing same to both parents. The hybrid plants are expected to increase the variability of fruit traits, and may have heat tolerance from L. gracilipes var. glabra.  相似文献