Detection of seven phytohormones in peanut tissues by ultra-highperformance liquid chromatography-triple quadrupole tandem mass spectrometry          下载免费PDF全文

WANG Hai-xia  WANG Ming-lun  WANG Xiu-zhong  DING Yu-long 《农业科学学报》2020,19(3):700-708

Development of highly sensitive and reliable method for detection of phytohormones is of great significance to study plant hormones and agricultural production.In this study,an ultra-high-performance liquid chromatography-mass spectrometry/mass spectrometry method was established for separation and quantification of trans-zeatin,trans-zeatin riboside,gibberellin A_3,indol-3-acetic acid,salicylic acid,abscisic acid,and jasmonic acid(JA) without any label.The sepa ration was performed on an Agilent Explus Plus C18 column by using methanol and water as mobile phases with gradient elution.The target compounds were confirmed and quantified by mass spectrum via positive electrospray ionization for trans-zeatin,transzeatin riboside,indole-3-acetic acid,and via negative electrospray ionization for gibberellin3,salicylic acid,abscisic acid,and JA.The limits of detection ranged from 0.0127 ng L~(-1) for gibberellin A_3(GA_3) to 33.26 ng L~(-1) for JA and were lower than the currently reported values in literature.The proposed method was applied for qualitative and quantitative analyses of phytohormones in peanut gynophores and pods.The recoveries of the spiked phytohormones ranged from 80.20 to102.56%.The contents of seven endogenous hormones varied specifically in different development stages of peanuts.This study provides a highly sensitive and selective detection method for hormones and elucidates the growth and development of the gynophore and peanut fruit,which are controlled by seven endogenous hormones.  相似文献   

Maize harvest index and water use efficiency can be improved by inhibition of gibberellin biosynthesis          下载免费PDF全文

B. W. Hütsch  S. Schubert 《Journal of Agronomy and Crop Science》2018,204(2):209-218

Possibilities to improve maize harvest index and nutrient utilization efficiency by application of plant growth regulators were investigated. In container experiments, the effects of different growth regulators on the development of the maize (Zea mays L.) cultivars Pioneer 3906 and Fabregas were tested. Paclobutrazol (PAC) and chlorocholine chloride (CCC), two inhibitors of gibberellin biosynthesis, as well as gibberellic acid (GA₃) were applied at growth stage V5. Three weeks after application of PAC, shoot growth of both maize cultivars was strongly affected with a significant decrease in plant height in the PAC treatment by 44% and 36% for Pioneer 3906 and Fabregas, respectively. The growth‐retarded plants had higher leaf areas and reduced transpiration rates. The higher shoot growth after GA₃ application was accompanied by a reduction in leaf area and an increase in transpiration rate during 1 week before anthesis. CCC treatment showed no significant effects on plant height, leaf area and transpiration rate. The PAC‐treated cultivar Pioneer 3906 produced several cobs per plant, which were mainly barren at maturity. However, PAC application to Fabregas resulted in just one cob per plant with good kernel development and a grain yield, which was not significantly reduced in comparison with the control. With this similar grain yield in combination with a straw yield decrease of 32%, the harvest index was significantly improved by 12%. In addition, with PAC‐treated Fabregas plants, a 19% increased water use efficiency of the grain (WUE_grain) during the critical period of kernel setting was achieved. In this maize cultivar, CCC application also improved harvest index by 5%, but no effect on WUE_grain occurred. GA₃ treatment decreased harvest index of both maize cultivars, and it either reduced WUE_grain (Pioneer 3906) or showed no effect (Fabregas). Utilization efficiencies of N, P and K were not increased with growth regulator application, even in the PAC‐treated Fabregas plants with a significantly improved allocation of assimilates to the grain, mirrored by the higher harvest index. The results indicate that fertilizer applications must be adjusted to the reduced demand of growth‐retarded plants, most likely leading to higher nutrient utilization efficiencies.  相似文献   

影响马铃薯块茎低温糖化的植物激素相关基因的表达     

阳芳  何高镜  郭圣军  刘清  胡新喜  贺利雄 《湖南农业大学学报(自然科学版)》2022,48(2):160-167

对低温贮藏5 d的低温糖化不敏感品种‘华薯三号’和敏感品种‘中薯五号’马铃薯块茎进行转录组测序,通过对差异表达基因的生物信息学分析,获得与植物激素相关的差异表达基因,其中6个与植物激素合成或信号转导有关的差异表达基因与糖分解代谢密切相关。结果表明：KOox2和GA3ox1是马铃薯块茎中赤霉素合成的关键酶基因,赤霉素可刺激淀粉酶和转化酶活性,加剧块茎还原糖的积累,是马铃薯低温糖化的正调控因子;ANT和AIL6属于参与乙烯响应的AP2转录因子家族;BRI1和BKI1是油菜素内酯信号通路中的转录因子,两者互作共同拮抗14–3–3蛋白质活性;ANT、AIL6、BRI1和BKI1这4个转录因子通过负调控转化酶基因的表达而影响还原糖的产生,是马铃薯低温糖化的负调控因子。  相似文献   

植物生长调节剂对树莓组培苗增殖生长的影响   总被引：2，自引：0，他引：2  

杨帆  张万博  朗贤波  廉美兰  朴炫春 《延边大学农学学报》2014,(1):12-15

以丰满红树莓组培苗为材料,研究了细胞分裂素、BA与生长素配比及赤霉素对树莓组培苗增殖生长的影响.结果表明,BA浓度为1.0 mg/L时,树莓的增殖效果显著好于KT（P＜0.05）;BA与IBA配合使用时效果更加明显,在BA 1.0 mg/L＋IBA 0.1 mg/L处理中,组培苗生长健壮且数量较多,增殖效果最佳;GA3浓度为4.0 mg/L时,组培苗鲜重和干重（156.4和34.1 mg）显著优于其它处理.  相似文献   

松树针叶束内源激素与单宁的变化     

郑彩霞  李凤兰  高荣孚  王瑞勤 《中国林学(英文版)》1996,(2)

本文分析和比较了油松、白皮松、华山松和樟子松各种针叶束内源激素与单宁含量的变化。结果表明，ＡＢＡ与单宁含量在树种间、树龄间和针叶束叶龄间均存在明显差异，针叶束经低温砂藏处理，其内源激素与单宁水平呈规律性变化，在处理前期，和树种针叶束的ＬＡＡ、ＧＡｓ、Ｚ和ＺＲ水平明显提高，然后随砂藏时间的延长而趋于降低；而ＡＢＡ与单宁水平则呈相反的变化，在砂藏过程中内源激素与单宁的变化规律与前期实验中经砂藏处理的离体针叶束不定根的诱导率紧密相关。结果表明，低温砂藏处理在一定时期可以促使松树针叶束内源激素与单宁水平的协调性变化，这种变化对离体针叶束不定根的诱导具有明显的促进作用  相似文献   

外源GA3、ABA对种子发芽与亲本穗上发芽内源激素变化影响研究     

周述波  贺立红  林伟 《种子》2011,30(5)

以外源GA3和ABA处理种子和水稻亲本,研究GA3和ABA对水稻种子萌发及亲本穗上发芽的影响,结果表明,外源GA3处理后引起两者种子内源GA3升高,降低种子内ABA、IAA水平,提高了GA3/ABA比值;外源ABA处理,引起种子内ABA含量升高,降低GA3、IAA水平,GA3/ABA比值减少;种子萌发和穗芽形成过程中GA3含量、GA3/ABA比值对比,发现前者小于后者,而种子萌发率与穗芽率相比,前者远远大于后者,表明穗上穗芽形成是一个更为复杂的生理变化过程.  相似文献   

钾对水稻籽粒发育中激素变化的影响   总被引：1，自引：0，他引：1  

袁玲  黄建国 《西南大学学报(自然科学版)》1993,(1)

在水稻籽粒成熟过程中,CTK、IAA、GA和ABA依次出现。其中,CTK的升降与初生胚和胚乳形成的时间基本吻合,ABA的高峰期与籽粒进入腊熟期的时间完全一致,说明籽粒的发育与成熟受内源激素的调节。钾显著降低了空壳率,可能是提高了CTK的含量,使受精卵的退化率降低;推迟了ABA出现的高峰期,使灌浆期延长,转移到籽粒中的碳水化合物增多,因而粒重加大。  相似文献   

ABA对杂交稻幼苗抗冷性机理的研究   总被引：5，自引：0，他引：5  

李光林  陈德万 《西南大学学报(自然科学版)》1994,16(2):138-143

杂交水稻幼苗３℃胁迫３６ｈ后，超氧物歧化酶活性和ＡＴＰ含量明显下降，膜脂过氧化作用加强，膜透性增大，当用１０＾５ｍｏｌ／ＬＡＢＡ预处理幼苗后，ＳＯＤ活性和ＡＴＰ含量下降受到抑制，膜脂过氧化作用减弱，膜透性降低，同位素示踪表明，ＡＢＡ使幼苗ＳＯＤ活性增强的原因是促进了ＳＯＤ的再合成，而不是激活其活性，同时证明，膜脂过氧化与ＳＯＤ活性的变化有着密切联系。  相似文献   

激素和多胺对苦瓜性别分化的影响   总被引：29，自引：2，他引：27  

汪俏梅  曾广文 《园艺学报》1997,24(1):48-52

研究了ＧＡ３（赤霉素）和ＣＣＣ（矮壮素）处理下株洲长白苦瓜植株，从苗龄８～７０天茎尖内源激素的变化。结果表明，第一雌花形成与茎尖内源ＧＡ３含量增高有关。开花盛期，茎尖内源ＩＡＡ含量下降越小，ＺＴ含量下降越大，植株的雌花数和♀／♂越大。分析表明，雌、雄花中内源多胺（ｃａｄ）含量都明显高于无性组织，可能与性别分化有关，内源亚精胺（ｓｐｄ）含量变化可能与雌花的发生和发育有关，内源腐胺（ｐｕｔ）含量的上升可能与雄花的分化有关  相似文献   

The Effectiveness of Vernalization of Immature Embryos of Winter Wheat var. Grana as Related to Age and Exogenous Phytohormones     

F. Dubert    I. Marci&#;ska    J. Biesaga-Ko&#;cielniak  I. Szmider 《Journal of Agronomy and Crop Science》1993,170(4):234-242

Starting from the 10th day after pollination, immature embryos of winter wheat var. Grana were isolated and then vernalized for 4 to 8 weeks on Murashige and Skoog nutrients containing BAP (0.5 or 2 mg/dm³), NAA (0.5 or 2 mg/dm³), or GA₃ (5 or 20 mg/dm³). Vernalized seedlings were cultivated in a greenhouse and the number of days to the heading of individual plants as well as the percentage of plants capable of generative development were estimated. The lower limit of size for 50 % survival of embryos strongly depended on the phytohormone used: from 0.9 mm in control, 1.1 mm in nutrient containing BAP, 1.2 mm for NAA, up to 1.7 mm in nutrient with GA₃. Exogenous GA₃ was lethal for embryos younger than 18 days but induced elongation of older embryos. Embryos isolated 2.5 to 4 weeks after pollination showed minimal requirements for the length of vernalization. BAP increased the percentage of heading plants originated from the youngest embryos. GA₃ improved partial vernalization, strongly increasing the percentage of heading plants, but did not change the time from the end of vernalization to heading. It has been postulated that GA₃ increases number of plants capable of overcoming the threshold of induction of generative development but does not accelerate the flowering process. Hormonized plants showed no deformation of generative development. As the effectiveness of vernalization increased, the heading of plants was faster but they were shorter, forming spikes with a smaller number of spikelets and producing fever lateral shoots. The very young embryos probably have in reserve sufficient amounts of auxins and gibberellins and therefore exogenous GA₃ decreases their viability or even exerts a deleterious effect. However, as the embryos' ageing, gibberellin starvation develops. This being especially pronounced during vernalization. The de novo synthesis or activation of gibberellins takes place during the second stage of vernalization. This is why exogenous hormone improves the effectiveness of partial vernalization, though it is not possible to substitute by gibberellins the vernalization requirements of immature embryos.  相似文献