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三环唑抑制稻瘟病菌MIC的测定及菌株产黑色素能力比较 总被引：2，自引：0，他引：2
测定了三环唑抑制黑龙江省116个稻瘟病菌菌株黑色素化的MIC值、黑色素合成量及三环唑抑制菌丝黑色素生物合成的有效中浓度(EC50-H),测定结果表明,黑龙江省稻瘟病菌菌株产黑色素的能力差异较大,产黑色素能力最强的菌株与产黑色素能力最弱的菌株的黑色素产值相差7.26倍。从三环唑抑制稻瘟病菌黑色素合成来看,绝大多数供试菌株对三环唑表现为较敏感。 相似文献
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Metalaxyl and tricyclazole are two fungicides widely used in Spain in vineyard and rice crops respectively. In this study an investigation has been made of the effect of three organic amendments [two commercial amendments, solid fertiormont (SF) and liquid fertiormont (LF), and a residue from the olive oil production industry, alperujo (OW)] on fungicide fate in soils. Changes in soil porosity on amendment were studied by mercury intrusion porosimetry, sorption-desorption studies were performed by the batch equilibration method, dissipation of metalaxyl and tricyclazole in the soil was studied at - 33 kPa moisture content and 20 degrees C and leaching was studied in hand-packed soil columns. Amendments with SF and LF reduced soil porosity, while OW increased porosity through an increase in pore volume in the highest range studied. Tricyclazole sorbed to soils to a much higher extent than metalaxyl. With some exceptions, sorption of both fungicides increased on amendment, especially in the case of SF-amended soils, which rendered the highest K(oc) values. In soils amended with the liquid amendment LF, sorption either remained unaffected or decreased, and this decrease was much higher in the case of metalaxyl and a soil with 70% clay. In this clay soil, amendment with OW, of very high soluble organic matter content, also decreased metalaxyl sorption. Tricyclazole is more persistent in soil than metalaxyl, and both fungicides were found to be more persistent in amended soils than in unamended soils. Leaching of metalaxyl and tricyclazole in soil columns was inversely related to sorption capacity. The low recoveries of tricyclazole in leachates and in soil columns when compared with metalaxyl, a less persistent fungicide, were attributed to diffusion into micropores and to increase in sorption with residence time in the soil, both processes favoured by the low mobility of tricyclazole. 相似文献
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为探究三环唑与二氯喹啉酸对稻田土壤细菌多样性和群落结构的影响,2021年在吉林省吉林市永吉县布设田间试验,利用高通量测序技术,研究推荐施用量（低剂量）和2倍推荐施用量（高剂量）对稻田土壤细菌群落的影响。结果表明：高剂量或低剂量的三环唑与二氯喹啉酸对细菌α多样性均无显著影响（P>0.05）,但对细菌群落结构产生显著影响（P<0.05）,且高剂量施用的影响大于低剂量;门水平注释结果显示,丰度前5的菌门为Proteobacteria（丰度占比为33.54%）、Chloroflexi(20.75%)、Acidobacteria(13.81%)、Bacteroidetes(9.86%)、Actinobacteria(8.48%),高剂量施用对菌门相对丰度的影响大于低剂量;对丰富类群和稀有类群的分析发现,施用不同剂量的三环唑和二氯喹啉酸均造成稀有类群减少,条件稀有类群增加;特化种分析结构显示,不同处理对共有特化种所属菌门丰度均造成影响,普遍规律为高剂量施用的影响大于低剂量施用,与二氯喹啉酸相比,三环唑的施用产生了更多的特化种,且其所属菌门分布更广泛,其中高剂量施用三环唑处理产生的特化种... 相似文献
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为明确DHN黑色素合成抑制剂三环唑对玉米大斑病菌Setosphaeria turcica致病力的影响,通过含毒介质系统研究了三环唑作用下玉米大斑病菌的黑色素生物合成量、附着胞的细胞孔径、膨压和病菌的致病力。结果显示,当三环唑浓度≤5μg/mL时,玉米大斑病菌菌落生长量和产孢量变化不显著,但黑色素的生成量降至对照菌株的43%;三环唑处理的病菌附着胞细胞壁孔径增大至2.7～3.3 nm,膨压降至3.75 MPa,侵染率下降37.5%,同时病斑面积显著减小。表明三环唑通过抑制玉米大斑病菌DHN黑色素合成,降低了病菌附着胞的膨压,从而导致致病力下降。 相似文献
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分析了三环唑和多菌灵对水稻稻瘟病毒力及对品种抗病性的影响,结果表明三环唑和多菌灵对水稻品种与稻瘟病菌互作有重要影响。当喷雾0.2μg/mL三环唑于13个日本已知抗性基因品种时,原对ZE3和ZG1混合菌株表现抗性的爱知旭表现感病,当喷雾2μg/mL三环唑时,爱知旭则表现抗病,而在0、0.2μg/mL三环唑时均表现抗病的露明则在2μg/mL三环唑时表现感病;喷雾0.2μg/mL多菌灵于13个日本已知抗性基因品种时,ZB1、ZE3、ZG1混合菌株在0μg/mL多菌灵时表现抗性的爱知旭则表现感病,当多菌灵浓度为2μg/mL时,露明又表现为抗病,而在0、0.2μg/mL多菌灵时,对ZG1表现为抗病的草笛则表现感病;13个已知抗性基因品种中其他品种在使用了三环唑和多菌灵后抗性也发生了变化,这说明稻瘟病菌的分布和组成与这类杀菌剂的长期使用可能有一定关系。 相似文献
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稻瘟病菌对异稻瘟净、多菌灵和三环唑的敏感性检测及抗药性变异研究 总被引：22，自引：0，他引：22
用区分剂量法检测了2000年采自中国广东、广西、安徽和江苏四省129株稻瘟病菌对异稻瘟净和多菌灵的抗药性频率。稻瘟病菌对生产上已停用10年左右的异稻瘟净的抗药性频率仍高达79.1%;在广东省高要市检测到了一例多菌灵抗药性菌株GY5,频率为0.78%。利用抑制菌丝黑色素化的最小抑制浓度（MIC）离体检测了稻瘟病菌对三环唑的敏感性,结果表明稻瘟病菌黑色素的生物合成对三环唑存在着不同的敏感性基因水平,但没有观察到上述MIC值与活体条件下测定的防治稻瘟病的[i]EC[/i][sub]50[/sub]间存在相关性。用药剂驯化和紫外诱变的方法都获得了稻瘟病菌抗异稻瘟净突变体和抗多菌灵突变体,但未能获得抗三环唑突变体。 相似文献
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Sensitivity Detection Technique and Resistance Risk Assessment of Magnaporthe grisea to Tricyclazole 总被引：1，自引：0，他引：1
ZHANGChuan-qing ZHOUMin_g-guo XUENa 《水稻科学》2005,12(1):68-74
In vitro detection method for the sensitivity of Magnaporthe grisea to tricyclazole was studied, and the potential resistance risk of blast disease to tricyclazole was assessed. Both EC5o of hyphal melanization (ECso-H) and minimum inhibitive concentration of melanization in appressorial (MIC-A) by inhibitor tricyclazole showed positive correlation to the EC5o of tricyclazole against blast disease tested in vivo, with relative co-efficiency (R2) of 0.8995 and 0.8244, respectively. However, stability and reproducibility of ECso-H were better than those of MIC-A, suggesting that it could be used to detect the sensitivity of M. grisea to tricyclazole in vitro. Tricyclazole sensitivity of the progenies derived from single spores of the most sensitive isolate DY2 and the least sensitive isolate GY6 detected in sensitivity monitoring in 2000 was not stable, with mean EC50 values of 4.4968μg/mL and 5.4010μg/mL, respectively, indicating that the difference in EC5o between DY2 and GY6 was not caused probably by resistance variation. EC5o of GY6 did not increase significantly when continuously selected for twenty generations under the selection pressure of tricyclazole in vivo. However, the sensitivity of DY2 was decreased by 10-fold after selected for twenty generations.The results suggested that tricyclazole was still low resistance risk for M. grisea in China. 相似文献
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稻瘟病菌对稻瘟灵、异稻瘟净和三环唑的敏感性 总被引：3，自引：1，他引：2
采用菌丝生长速率法分别测定了57株稻瘟病菌对稻瘟灵和79株稻瘟病菌对异稻瘟净的敏感性,分别建立了敏感基线,并检测了141株大田稻瘟病菌对稻瘟灵和异稻瘟净的抗药性水平;采用活体植株法测定了223株稻瘟病菌对三环唑的敏感性。57株稻瘟病菌对稻瘟灵的平均EC50值为1.4312±0.0867μg/mL,其敏感性频率分布呈连续性单峰曲线,1.4312μg/mL可作为稻瘟病菌对稻瘟灵的敏感基线,在141株大田稻瘟病菌中敏感、低抗和中抗菌株出现的频率分别为21.99%、73.76%和4.26%;79株稻瘟病菌对异稻瘟净的平均EC50值为6.6393±0.2662μg/mL,其敏感性频率分布也呈连续性单峰曲线,6.6393μg/mL可作为稻瘟病菌对异稻瘟净的敏感基线,141株大田稻瘟病菌中敏感、低抗和中抗菌株出现的频率分别为90.07%、7.80%和2.13%。稻瘟病菌对三环唑的EC50值最大为47.6144μg/mL,最小为1.0850μg/mL,供试菌株的EC50值呈近似正态分布,未出现敏感性下降的抗药性亚群体。 相似文献
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采用菌落生长抑制率法,分析了黑龙江省不同地区稻瘟病菌菌株对三环唑的敏感性.结果表明:三环唑对黑龙江省不同地区稻瘟病菌的平均抑制中浓度(Ec50)有差异,大多数分布在160～190 mg·L1,且400 mg·L-以上的三环唑才能有效抑制稻瘟病菌的生长.同时还发现尚志、富锦地区、佳木斯、萝北、连珠山、北林区、宁安江南地区... 相似文献