马铃薯热激转录因子HsfA3基因的克隆及其耐热性功能分析     

唐锐敏  贾小云  朱文娇  印敬明  杨清 《作物学报》2021,(4):672-683

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Expression of Anti-Lipopolysaccharide Factor Isoform 3 in Chlamydomonas reinhardtii Showing High Antimicrobial Activity     

Anguo Li  Ruihao Huang  Chaogang Wang  Qunju Hu  Hui Li  Xiao Li 《Marine drugs》2021,19(5)

Antimicrobial peptides are a class of proteins with antibacterial functions. In this study, the anti-lipopolysaccharide factor isoform 3 gene (ALFPm3), encoding an antimicrobial peptide from Penaeus monodon with a super activity was expressed in Chlamydomonas reinhardtii, which would develop a microalga strain that can be used for the antimicrobial peptide production. To construct the expression cluster, namely pH2A-Pm3, the codon optimized ALFPm3 gene was fused with the ble reporter by 2A peptide and inserted into pH124 vector. The glass-bead method was performed to transform pH2A-Pm3 into C. reinhardtii CC-849. In addition to 8 μg/mL zeocin resistance selection, the C. reinhardtii transformants were further confirmed by genomic PCR and RT-PCR. Western blot analysis showed that the C. reinhardtii-derived ALFPm3 (cALFPm3) was successfully expressed in C. reinhardtii transformants and accounted for 0.35% of the total soluble protein (TSP). Furthermore, the results of antibacterial assay revealed that the cALFPm3 could significantly inhibit the growth of a variety of bacteria, including both Gram-negative bacteria and Gram-positive bacteria at a concentration of 0.77 μM. Especially, the inhibition could last longer than 24 h, which performed better than ampicillin. Hence, this study successfully developed a transgenic C. reinhardtii strain, which can produce the active ALFPm3 driven from P. monodon, providing a potential strategy to use C. reinhardtii as the cell factory to produce antimicrobial peptides.  相似文献   

NO对大鼠中脑腹侧被盖区DA神经元的调节     

孙碧英  张文慧 《动物医学进展》2003,24(4):66-68

实验观察了微量注射 NOS抑制剂 L -NAME及微量联合注射 NO的前体 L-精氨酸( L-Arg) L-NAME于大鼠中脑腹侧被盖区( VTA)对该部位多巴胺神经元的调节。发现VTA注射 L -NAME( 1 mg/ 5μL )后 ,伏隔核( Acb)多巴胺 ( DA )代谢产物—双羟苯乙酸( DOPAC)水平升高到注射前的 1 2 2 .5% ( P <0 .0 0 1 ) ,小剂量注射 L-NAME( 0 .2 mg/ 5μL)对伏隔核 DOPAC水平无明显影响 ;同样方法联合注射 L-Arg( 3 0 0 μg/ 5μL) L-NAME( 1 mg/5μL)后 ,伏隔核 DOPAC水平无明显变化。结论 :VTA微量注射 L-NAME兴奋了该部位的DA神经元 ,而 L -Arg L -NAME联合注射 ,却不能影响 DA神经元的活动 ,说明 NO可以通过L-Arg-NOS-NO途径参与 VTA多巴胺神经元的调节  相似文献   

茶多酚(TP)对家禽免疫功能的研究     

詹勇  李进昌  杨贤强 《浙江大学学报(农业与生命科学版)》1992,(4)

选用14日龄健康的海佩科内用仔鸡48羽,随机分成试验(24羽)和对照(24羽)两组.试验组在饲粮中添加0.25％的茶多酚(TP),研究TP对鸡免疫功能的作用.试验结果表明,TP对自然感染法氏囊病鸡,有明显提高(p<0.05)鸡血液中红细胞C_(3b)受体和免疫复合物的含量及保护鸡脾脏正常的免疫功能,同时也发现TP对公母鸡法氏囊的重量有不同影响.本试验表明,TP具有提高家禽免疫功能的作用.  相似文献   

菜用加工兼用栗永荆3号选育     

阮逸  李德静  叶茂宗 《浙江林业科技》2001,21(5):27-29

一般认为我国南方栗仅宜于菜用，加工极易开瓣和糊化，经过几年的筛选和加工试剂，永荆3号品种可生产B－E级标准的糖水栗子罐头。  相似文献   

基于模糊建模的混炼胶粘度测量     

樊绍胜  王耀南 《湖南农业大学学报(自然科学版)》2004,31(3)

提出了一种在线测量混炼胶粘度的新方法．该方法以模糊建模技术为基础，综合考虑混炼过程各因素对胶料粘度的影响，建立起胶料粘度的在线测量模型．在模糊建模中，采用T-S模型描述胶料粘度变化的非线性过程，提出了一种基于相似性判别的模糊聚类算法以自动确定合适的聚类组数目，并用实数编码的遗传算法优化全局参数，从而获得了规则简化的、具有较高精度的模糊模型．根据此方法，设计了测量装置，并进行了现场试验．试验结果表明模糊模型输出与实验室测量值基本一致，平均误差较低且最大误差未超过1门尼．该方法较大地提高了橡胶混炼的生产效率，为粘度最优控制奠定了基础．  相似文献   

High frequency embryogenic callus induction and plant regeneration from mature caryposis of big bluestem and little bluestem     

Yonghong Li  Junping Gao  Shui-zhang Fei 《Scientia Horticulturae》2009

Big bluestem (Andropogon gerardii Vitman) and little bluestem [Schizachyrium scoparium (Michaux) Nash.] are native to the North America and are important forage grasses and ornamental grasses. Both grasses are proposed as ideal biomass producers for cellulosic ethanol production. To apply genetic transformation, which is an important tool for incorporating desirable agronomic traits into plants to both species, however requires an efficient and reproducible regeneration protocol. We used mature caryopses from big and little bluestem as explants and tested the effect of various combinations of 2, 4-dichlorophenoxyacetic acid (2, 4-D) (1, 2, 3, 4 or 5 mg l⁻¹) and kinetin (KT) (0, 0.1 or 0.2 mg l⁻¹) on embryogenic callus induction with LS as the basal medium. The highest percentage of embryogenic calli induction occurred on medium containing 2, 4-D alone at 2 mg l⁻¹ for ‘Bison’ and on medium containing 4 mg l⁻¹ 2, 4-D alone for ‘Bonilla’ big bluestem. For little bluestem, the highest percentage of embryogenic callus induction occurred on medium containing 3 mg l⁻¹ 2, 4-D plus 0.1 mg l⁻¹ kinetin, suggesting that addition of KT is beneficial. Shoot regeneration took place on LS basal medium without any plant growth regulator for both species, although the addition of KT increased both regeneration frequency and the number of shoots produced per callus. Rooting of shoots reaching about 2 cm long occurred readily with or without α-naphthaleneacetic acid (NAA). Rooted plantlets were all successfully established in the soil.  相似文献   

管道3PE防腐涂层补口技术标准及规范对比分析     

赵君  侯宇  张丰  王德增  刘玲莉  丁峰 《油气储运》2009,28(3)

通过对比国内外热收缩带(套)补口技术规范及标准,分析了各项性能指标间的差异,包括材料性能指标、安装工艺及质量检验.指出管道表面处理及底漆涂装是影响热收缩带(套)安装系统性能的重要因素,提出了工程技术规范编制建议.  相似文献   

"数字农业"及其实现技术   总被引：13，自引：2，他引：11  

于淑惠 《农业图书情报学刊》2004,15(7):5-7,17

文章介绍了“数字农业”的含义、特点以及其所涉及的技术体系，说明了它对我国农业发展具有重大意义。  相似文献   

Boundary-fitted grid in landscape modeling     

Kang-Ren Jin  Yegang Wu 《Landscape Ecology》1997,12(1):19-26

Landscape modeling requires the delineation of system boundaries and interior features. Quite often, these components are complex and difficult to accurately represent. A rectangular grid is used to represent the study and adjacent non-study areas in most cases. When the non-study area occupies a large portion of the grid, computer memory is wasted, and computational time increases. An elliptical grid generator for non-orthogonal curvilinear coordinates is used to generate a boundary-fitted grid for a landscape model. In a boundary-fitted grid coordinate system, one coordinate axis follows the landscape domain boundary and is non-orthogonal to the second axis. The boundary-fitted grid uses elliptic partial differential equations to distribute grid points inside the landscape domain. Although the boundary-fitted grid follows the domain boundary, the grid pattern and point allocation remain structured. Thus, a landscape model can use a boundary-fitted grid without changing the model’s data structure or the computational scheme. In this study, a boundary-fitted grid and a raster-based grid were applied to the Everglades Landscape Fire Model. Use of the boundary-fitted grid decreased model simulation time by about one fifth and computer storage by 58% relative to the raster-based grid. Also, the linear characteristics of interior geographical features such as rivers and airboat trails were preserved by the boundary-fitted grid, but not by the raster-based grid. This preservation provided a more reasonable base map for simulating ecological processes, such as fire across heterogenous landscapes.  相似文献