Detect unique molecular structure associated with physiochemical properties in CDC varieties of oat grain with unique nutrient traits [Feed Type vs. Milling Type] in comparison with barley grain using advanced molecular spectroscopy as a non-destructive biological tool     

《Journal of Cereal Science》2017

The objective of this study was to determinate grain unique protein inherent molecular structure that are related physiochemical and nutrient profiles in CDC developed oat varieties [CDC Nasser (Feed Type) and CDC Seabiscuit (Milling Type)] grown in cool climate condition in western Canada in comparison with conventional barley variety of CDC Meredith as a control using advanced molecular spectroscopy. Multivariate analyses, including an agglomerative hierarchical cluster analysis (CLA) and principal component analysis (PCA), were performed to identify protein molecular structural differences among the grains. The results revealed that CDC Seabiscuit contained greater (P < 0.05) protein structural Amide I and II than CDC Nasser and CDC Meredith, while the greater (P < 0.005) structural Amide I to II area and height ratios was detected in CDC Meredith. New oat grains had greater (P < 0.05) β-sheet height than barley grains, however, there was no difference in α-helix to β-sheet ratio values among the varieties. In conclusion, CDC Nasser and CDC Meredith had no difference in protein molecular structural features, while CDC Seabiscuit contains different protein structural characteristics as compared to CDC Meredith grain. The molecular structure features are highly associated with physiochemical and nutrient profiles in grains, which indicate that it also affect nutrient utilization and availability.  相似文献   

Identification of an H1N1 subtype of swine influenza virus and serological analysis     

Fa-chao SUN  Min TAN  Yuan-chao ZHANG  Yu-chao WANG  Sheng-liang CAO  Guo-fei DING  Fang-yuan CONG  Li-hong GUO  Si-dang LIU  Yi-hong XIAO 《农业科学学报》2019,18(7):1436-1442

To investigate the epizootic of swine influenza virus(SIV), 60 nasal swabs were collected from a clinical cases of pig farm in Tai'an City, Shandong Province of China in April 2017. SIV was isolated by inoculating into 10-day-old Special Pathogen Free embryonated eggs and the whole genome was sequenced. An H1N1 subtype SIV was isolated and designated as A/swine/Shandong/TA04/2017(H1N1). Phylogenetic analysis showed that apart from the polymerase A(PA) fragment belonging to the 2009 pandemic H1N1 branch, seven genome segments belonged to avian-like H1N1 influenza virus lineage. The cleavage site sequence of the hemagglutinin(HA) protein was PSIQSR↓G, which is a typical molecular biological characteristic. Five potential N-glycosylation sites(N14, N26, N277, N484 and N543) were found in the HA gene. To further investigate the epidemiology of SIV in this farm, the 995 serum samples were assessed with EAH1N1 2009 pandemic H1N1 and H3 N2 antigens. The results showed that the total positive rate was 65.43%. The positive rates of single virus infection detected by EAH1N1, 2009 pdmH1N1 and H3 N2 for serum HI(Hemagglutination inhibition) were 48.35, 30.85 and 7.47%, respectively. The results showed that SIV in Shandong Province has been reassorted in some segments and the SIV-positive rate was high on the SIV outbreak farm. These data provide evidence of an epizootic of SIV.  相似文献   

小叶杨基因资源异地保存及苗期生物学特性分析     

付贵生  杨自湘  马金昌  张军红 《林业科技》2005,30(1):1-4

分别从东北、西北和华北收集小叶杨各类资源。并建立了异地保存基因库。同时，对小叶杨资源苗期的生长、物候期、形态特征以及同工酶谱进行了观测和分析。  相似文献   

初冬早春南昌郊区灯下常见蛾类活动情况纪实     

章士美  赵凤霞 《江西农业大学学报》1994,16(1):47-51

  相似文献   

作物活体多探头测量仪的研制     

罗时石  龚荐  葛才林  张浩  彭永欣  封超年  郭文善 《扬州大学学报(农业与生命科学版)》1991,(4)

采用TP—801单板机制作作物活体多探头测量仪。该机可在田间对生长状态下的农作物进行多点体内养分积累、运输、分配等测定,且结果可靠。  相似文献   

新疆农田土壤有机质含量及组成特征   总被引：3，自引：0，他引：3  

姜益娟  郑德明  吕双庆  朱朝阳  伍维模  柳维杨 《干旱地区农业研究》2003,21(4):61-64

目前新疆农田土壤有机质含量在5．30～24．21g／kg之间，平均14．65g／kg，与第二次土壤普查相比，新疆农田土壤有机质含量呈上升趋势；胡敏素碳含量占到有机质碳量的53％～70％，平均61％，腐殖酸碳量只占39％。  相似文献   

蔬菜作物多倍体育种研究进展   总被引：19，自引：0，他引：19  

刘文革  王鸣  阎志红 《长江蔬菜》2003,(1):29-33

对蔬菜作物多倍体育种研究的主要进展进行了综述。重点对蔬菜多倍体的 获得途径、多倍体的植物学特征、同源三倍体的不育性、四倍体的低稔性、抗逆性研究进行了综述，介绍了多倍体在蔬菜育种上的应用，并提出了蔬菜多倍体育种尚待解决 的关键问题。  相似文献   

二丁酰环腺苷酸对猪胴体组成和肉品质的影响   总被引：1，自引：0，他引：1  

张曦  高士争  程美玲  刘勇  严达伟 《中国畜牧杂志》2004,40(5):20-22

通过皮下注射和饲料添加不同剂量dbcAMP进行饲养试验 ,研究其对肥育猪胴体组成和肉品质的影响。结果表明 :皮下注射 1.0mg/kg和饲料添加 2 0mg/kg对改善肥育猪的胴体组成和肉品质效果最好 ,眼肌面积分别提高31.99%和 36 .39% (P <0 .0 5 ) ;瘦肉率分别提高 12 .4 2 %和 11.94 % (P <0 .0 5 ) ;背膘厚分别降低 19.95 %和 2 0 .97%(P <0 .0 5 )。肌肉粗蛋白含量分别提高 10 .87%和 16 .5 4 % (P <0 .0 5 )。  相似文献   

封闭型植物工厂发展现状与展望     

余锡寿  刘跃萍 《农业展望》2015,(7)

经过多年探索和发展,封闭型植物工厂无论在材料、装置和技术都取得了快速进步,生产产量成数倍增长。在介绍发展封闭型植物工厂必要性的基础上,着重分析了封闭型植物工厂的发展现状、设施分类、栽培形式选择、栽培技术选择,最后指出了存在的问题,并对未来的封闭型植物工厂外部设施及其栽培技术进行了展望。  相似文献   

基于GF-6卫星影像多特征优选的酿酒葡萄精准识别   总被引：2，自引：2，他引：0  

李文杰  郭晓雷  杨玲波  闫鸣  邹晨曦  方亚华  孙涵  黄敬峰 《农业工程学报》2020,36(18):165-173

多源遥感信息和特征优选是提高农作物识别精度的重要支撑，高分六号（GF-6）卫星作为首次引入红边波段的国产卫星，其丰富的光谱信息为作物识别提供了新的思路和解决途径。该研究基于宁夏回族自治区银川市永宁县2018年6月-2019年3月的GF-6数据，充分利用红边优势提取光谱特征、纹理特征和植被指数特征，构建多种特征组合方案，并根据随机森林算法对特征重要性进行度量，选取最优特征组合对酿酒葡萄进行精准识别。结果表明，与单一特征相比，多源遥感特征的增加显著改善了酿酒葡萄分类效果，其中，植被指数贡献程度最大，光谱特征次之；基于随机森林的优选特征组合分类效果最佳，其中，总体分类精度为94.15%，酿酒葡萄用户精度为94.23%，制图精度为92.59%；以实地调查的4个酒庄为验证区，将酿酒葡萄提取结果与统计数据进行对比，面积相对精度均在70%以上，其中优选特征结果相对精度在90%以上，研究结果将为国产卫星红边波段在植被分类和识别方面的应用提供数据参考。  相似文献