Mass Production of Freshwater Rotifer,Brachionus calyciflorus,Under Different Diets and Regimes     

《Journal Of Applied Aquaculture》2013,25(3):101-111

ABSTRACT

The production ability of freshwater rotifer, Brachionus calyciflorus, cultured with two algal species, Chlorella vulgaris, and Spirulina platensis, and a baker's yeast, Saccharomyces cerevisiae, were studied using semicontinuous culture method. B. calyciflorus was fed with the above three types of food at five different concentrations (125, 250, 500,750, and 1,000 μg/mL). Among the three different types of diet, maximum production of B. calyciflorus (489.20 ±10.91 individuals/mL; P < 0.05) was obtained with C. vulgaris, followed by S. cerevisiae, and S. platensis. Moreover, in each of the three diets, the maximum rotifer production was obtained at a particular concentration (C. vulgaris, 750 μg/mL; S. cerevisiae, 750 μg/mL; and S. platensis, 500 μg/mL) beyond which the rotifer production decreased. The peak production due to C. vulgaris (489 individuals/mL) was better than S. cerevisiae (321 individuals/mL) when the number of rotifers was considered. The present study indicates that the quantity and quality of food have a significant role on the rotifer production and that C. vulgaris at 750 μg/mL appears suitable to feed to rotifers for maximal production.  相似文献   

Dietary carotenoids and skin melanin content influence the coloration of farmed red porgy (Pagrus pagrus)     

S. CHATZIFOTIS  I. Vaz JUAN  P. KYRIAZI  P. DIVANACH  M. PAVLIDIS 《Aquaculture Nutrition》2011,17(2):e90-e100

The present study investigates the effects of dietary carotenoid sources on the coloration of the red porgy, Pagrus pagrus. Red porgies (131.9 ± 16.2 g; mean ± SD) were fed for 12 weeks on five different diets supplemented with red carotenoids (mainly astaxanthin esters) supplied from Haematococcus pluvialis algae and yellow carotenoids (mainly β‐carotene, lutein, and zeaxanthin) supplied from Alfalfa, Medicago sativa L. and Spirulina, Spirulina pacifica. The carotenoid‐supplemented diets did not have any marked effect on the growth rate, the feed conversion ratio, the daily feeding rate, or the hepatosomatic index of red porgy. The biochemical indices measured in plasma including cholesterol, total proteins, glucose, lactate, phospholipids, non‐esterified fatty acids, triglycerides, and thyroid hormones did not differ significantly among groups. Diet did not affect significantly the melanophore‐area coverage, the melanin skin concentration and skin lightness. Carotenoid‐supplemented diets affected significantly the carotenoid deposition in the skin, the presence and distribution of erythorphores and xanthophores, and skin hue and chroma. Overall, data have shown the efficacy of Haematococcus algae in promoting a reddish coloration in red porgy.  相似文献   

添加碳酸钙后螺旋藻中钙元素的分布及主要生化组成的变化     

郑江  高亚辉  韦青阳  欧阳丽佳  唐川宁  谢明艺 《中国水产科学》2004,11(5):462-466

通过向螺旋藻培养液中添加固体CaCO3粉末的方法,获得了富钙螺旋藻样品。采用原子吸收光谱法对螺旋藻的细胞壁和生化组分进行钙含量分析,结果表明,细胞壁在钙的富集过程中起了决定性的作用,螺旋藻细胞吸收富集的钙中,约有96%结合在细胞壁上;而糖类物质在有机钙的形成方面起了较为主要的作用,约有88%的有机钙与糖类物质结合,螺旋藻的生物富钙机制可能主要依靠细胞壁的吸附作用以及糖类物质的离子交换和主动运输来实现。对螺旋藻的生化分析显示,螺旋藻富集钙后,糖和蛋白质含量有所下降,而叶绿素a和类胡萝卜素含量则有较大地提高,前者可能与细胞壁的通透性及钙信号的刺激有关,后者则可能与螺旋藻自身的适应性调节作用有关。  相似文献   

基于nanoUPLC-MS/MS的螺旋藻游离肽组成分析     

褚宁宁  陈雨豪  陈娟娟  马斌  陈海敏  骆其君 《核农学报》2020,34(4):735-744

为了研究螺旋藻中的多肽成分,以钝顶螺旋藻为原料,采用超滤法提取螺旋藻中的游离肽,利用nanoUPLC-MS/MS和PEAKS Studio分析谱图信息,结合NCBI数据库进行比对和从头测序分析,获得游离肽的结构组成和百分含量信息。结果表明,利用数据库比对法和从头测序法,分别得到可信的游离肽4 485个和20 597个,匹配到的蛋白质有1 036种。数据库比对结果中的游离肽主要为七肽到二十一肽,少量为二十一肽以上;从头测序结果中游离肽主要为二肽到十六肽,少量为十六肽以上。数据库比对结果中百分含量最高的游离肽是十肽,为15.95%;从头测序结果中百分含量最高的是五肽,达到24.09%。本研究结果为螺旋藻蛋白资源的进一步开发和利用提供了依据。  相似文献   

无机与有机碳源混合营养培养对钝顶螺旋藻生长的影响   总被引：1，自引：0，他引：1  

田辉  谯顺彬  彭晓东  李林  张义明 《贵州农业科学》2011,(10):122-124

为深入考察碳源混合营养对螺旋藻生长的影响,采用响应面分析法对螺旋藻混合营养培养中的无机碳源(NaHCO3)与有机碳源(葡萄糖)进行2因素3水平试验.结果表明:单因素试验螺旋藻在基础培养基条件下,添加1.5g/L葡萄糖时,最终生物量为1.242 9 g/L;在以最佳葡萄糖添加浓度时,NaHCO3的最佳添加浓度为8.4g/...  相似文献   

钝顶螺旋藻藻蓝蛋白的提取及分子量研究   总被引：1，自引：0，他引：1  

张厚森  尹利端  赵厚民  贾涛  李新丽 《江西农业学报》2012,(10):102-103,126

以藻蓝蛋白(CPC)的提取率为指标,用冻融法对藻蓝蛋白进行提取,最后用SDS-PAGE和排阻色谱法对藻蓝蛋白的分子量分别进行了测定,结果表明,冻融法提取藻蓝蛋白的最优工艺参数为:料液比为1∶22,提取温度35℃,提取时间2.5h,藻蓝蛋白的提取率为3.19%。SDS-PAGE法测得藻蓝蛋白的分子量为40000 Da,排阻色谱法测得藻蓝蛋白的分子量为43200 Da,这2种方法所得的CPC蛋白分子量基本一致。  相似文献   

鄂尔多斯沙区碱湖与螺旋藻资源   总被引：15，自引：1，他引：15  

乔辰  李博生  曾昭琪 《干旱区资源与环境》2001,15(4):86-91

通过 1 995 - 2 0 0 1年长期对内蒙古鄂尔多斯高原毛乌素沙地和库布齐沙漠的八个盐碱湖进行了实地调研 ,在其中四个碱湖中发现有四种螺旋藻 ,其中两个为新种 ,另两个为中国新记录种。作者在巴彦淖尔碱湖畔建成半封闭跑道式养殖池 ,以当地的钝顶螺旋藻为藻种 ,利用湖水养殖螺旋藻获得了成功。藻粉产量达到 6 - 8g/d· m2 ,质量通过了内蒙古卫生防疫站“食准字”检验。作者在螺旋藻养殖上的成功 ,为其产业化奠定了基础。在鄂尔多斯沙区发展螺旋藻产业有着得天独厚的优势 :众多的碱湖资源 :特有的螺旋藻资源 ;丰富的光能资源 ;不占用耕地和牧场 ;不污染环境、可持续发展等。所以 ,在鄂尔多斯沙区开发螺旋藻是继化工产业化之后的又一项新兴产业  相似文献   

影响螺旋藻生长因素的研究进展   总被引：5，自引：0，他引：5  

田其英 《畜牧与饲料科学》2009,30(6):113-115

螺旋藻具有丰富和全面的营养,同时具有重要的医疗保健价值,有着广阔的应用前景.笔者主要论述了不同的螺旋藻培养条件对其生长和产物的影响.  相似文献   

Effect of Spirulina fusiformis on cyclophosphamide and mitomycin-C induced genotoxicity and oxidative stress in mice     

K. Premkumar  A. Pachiappan  Suresh K. Abraham  S. T. Santhiya  P. M. Gopinath  A. Ramesh   《Fitoterapia》2001,72(8):398-911

Spirulina fusiformis was tested for its possible in vivo protective effects against cyclophosphamide (CP) and mitomycin-C (MMC) induced genotoxicity and oxidative stress in mice. Pre-treatment with S. fusiformis (250, 500 and 1000 mg kg(-1), p.o., daily for 5 days) significantly reduced the chromosomal damage and lipid peroxidation with concomitant changes in antioxidants and detoxification systems. All the three tested doses were effective in exerting a protective effect against CP and MMC.  相似文献   

螺旋藻直线形变异藻株的生理特性研究     

赵洪新  陈盈  华秀英  张瑾  孙军德 《水产科学》2004,23(11):9-12

对钝顶螺旋藻进行研究和应用过程中分离筛选，获得一直线形变异藻株(Sp-L)。长期观察培养发现，分纯得到的直线形藻株能保持稳定遗传。对其形态、生长曲线、产量、上浮性、蛋白质含量以及常见金属元素(Fe、Mn、Cu、Zn、Ca、Mg)含量进行测定，结果表明直线形变异藻株具有藻丝体长、蛋白质和常见金属元素含量与正常螺旋藻接近及易于采收等特点，为研究直线形螺旋藻提供了基础性的理论依据。  相似文献