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Thomas K. Day DVM MS Susan Holcombe VMD William W. Muir II DVM PhD 《Journal of Veterinary Emergency and Critical Care》1993,3(2):90-95
A six-year-old arabian stallion was admitted to The Ohio State University Veterinary Hospital for evaluation and repair of a comminuted fracture of the second phalanx. The horse developed impaired arterial oxygenation during surgey and pulmonary edema post-operatively. We postulate that impaired arterial oxygenation resulted from atelectasis of the dependent lung during annesthesia, and the pulmonary edema occurred following re-expansion of the atelectatic of the initiating cause of the edema, removal of excess lung water from the alveoli, and restoration of normal arterial oxygenation. The horse was fully recovered within 12 hours of initiation of clinical signs of respiratory compromise. The horse was fully recovered within 12 hours of initiation of clinical signs of respiratory compromise. This report describes re-expansion pulmonary edema due to reperfusion injury in a horse, treatment of the condition, and a possible explanation of the pathogenesis of this pulmonary pathology. 相似文献
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新城疫(ND)—减蛋综合征(EDS76)蜂胶二联灭活疫苗的研制Ⅰ.制苗工艺、疫苗安全性和免疫原性试验 总被引:6,自引:0,他引:6
将新城疫病毒(NDV)Colon-30株和减蛋综合征病毒(EDS76AV)-127株分别接种于非免疫鸡胚和鸭胚,制备抗原液。抗原液经甲醛灭活后,与纯化的天然免疫增强剂蜂胶乳化,制成鸡新城疫(ND)-减蛋综合征(EDS76)蜂胶二联灭活疫苗,对疫苗的安全性和免疫原性进行了测定。对3-6周龄易感雏鸡以2倍使用剂量肌内注射,安全性良好;对1日龄雏鸡接种1个使用剂量进行急性毒性试验。15d生长曲线表明,对雏难 影响。对6周龄雏鸡注射1个使用剂量,每批疫苗免疫10只鸡,免疫后5-32d,鸡血清中EDS76血凝抑制(HI)抗体的几何平均滴度(GMT)为6.5-11.8log2,ND血凝抑制(HI)抗体的几何平均滴度(GMT)为4.8-9.8log2;对ND免疫攻毒试验表明,3批疫苗,每羽份含有的半数保护量(PD50)分别为≥127、≥131和≥127。 相似文献
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橡胶树低温伤害的生理反应 总被引:4,自引:1,他引:3
在人工零上低温下,巴西橡胶树(Hevea brasiliensis)叶质膜透性随低温处理时间的延长而持续上升,抗冷品系的上升速率比不抗冷品系慢。呼吸强度和ATP含量均随处理时间的延长而持续下降,抗冷品系下降速率比不抗冷品系慢。叶绿体Mg~(++)—ATPase活性也表现出明显抗性差异。可见,低温下叶组织的质膜透性、呼吸强度、ATP含量以及Mg~(++)—ATPase活性的变化与品系抗冷性有关。低温下呼吸强度、ATP含量与质膜透性变化呈负相关,质膜透性的变化与供能有关。 相似文献
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苹果采前落果与内源激素的关系 总被引:1,自引:0,他引:1
为了探讨苹果采前落果与内源激素之间的关系,在采前8周间定期测定了不同苹果品种的果柄、果台和离层形成部位组织中IAA、ABA含量;在采收前20d内定期测定了离层部位组织中细胞壁分解酶(Cellulase)的活性;在收获期测定了果实乙烯的发生量。结果表明:不同品种果柄、果台和离层部位组织中IAA和ABA含量变化有差异,但它们变化的总趋势相似,都是随着果实成熟IAA含量下降,而ABA含量上升;不同品种的成熟果实中乙烯发生量有很大差异,以落果多的品种显著大于落果少的品种;采前落果重的品种离层部位组织中细胞壁分解酶的活性在果实成熟期急剧增加。由于果实进入成熟阶段后,IAA含量下降,ABA含量升高,ABA/IAA之间的相对平衡被打破,高ABA/IAA以及高乙烯会刺激离层组织中细胞壁分解酶的活性增高,进而促进离层形成,这可能是导致落果发生的原因。 相似文献
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AIM:To study influence of ischemia-reperfusion(IR) on apoptosis and expression of apoptosis-related genes Fas-L, Bax and Bcl-2 of sinoatrial node(SAN) cells in rabbits in vivo. METHODS:Ninety healthy adult rabbits were divided randomly into control group, ischemia groups (I10 min, I30 min, I60 min and I120 min) and IR groups (I10 minR4h, I30 minR4h, I60 minR4hand I120 minR4h). IR injury model of SAN was established by occluding and loosening the start section of right coronary artery. The apoptosis of SAN cells was detected by TUNEL staining. The expression of Fas-L, Bax and Bcl-2 of SAN cells was detected by immunohistochemistry. RESULTS:①No obvious apoptosis of SAN cells was observed in control group, I10 min and I30 min groups. Apoptosis of different degrees in SAN cells were found in 68.3%(41/60) rabbits in I60 min, I120 min and 4 subgroups of IR. ②The highest expression of Fas-L and Bax was observed in I120 min group and that of Bcl-2 was in I60 min group. ③The highest expression of Fas-L and Bax was observed in I60 minR4h group. The peak level of Bcl-2 was observed in I30 minR4h group. ④The expression of Fas-L and Bax was significant higher in IR group than that in ischemic group at the same time point. CONCLUSION:Ischemia and IR induced apoptosis of SAN cells in rabbit in vivo. Fas-L、Bax、Bcl-2 may participate in the regulation of apoptosis and the injury during IR aggravates the apoptosis of SAN cells. 相似文献
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AIM:To study the relationship between myocardial HSP70 and PKC during myocardial ischemic preconditioning(IPC). METHODS: PKC inhibitor, polymyxin B(PMB) and PKC activator, phorbol 12-myristate 13-acetate(PMA) were applied to the models of myocardial ischemia/reperfusion in vivo and in vitro in rabbits, respectively, and the ventricular functions, PLA2 in the serum, and the expression of mycardial HSP70 mRNA were examined. RESULTS:IPC decreased PLA2 activity, improved the left ventricular function and increased the expression of myocardial HSP70 mRNA. Howerer, all these effects of IPC could be blocked by PMB. Interestingly, PMA mimicked IPC with attenuating the injuries of cardial myocytes and increasing myocardial HSP70 mRNA expression. CONCLUSION:PKC is involved in the myocardial HSP70 expression in case of ischemic preconditioning. 相似文献
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AIM: To investigate the effect of Sini decoction (SND)on the ultrastructure of small intestinal epithelial cells in rats with intestinal ischemia-reperfusion. METHODS: Thirty-two Sprague-Dawley rats of both sexes were randomly divided into 4 groups: (1) Control group in which sham operation was performed; (2) Model group in which intestinal I/R was produced by clamping super mesenteric artery(SMA) for 1 hour and declamping SMA for 3 hours; (3) SND1 group in which SD (0.6 g/200 g rat) was given via stomach tube 3 d before intestinal I/R; (4) SND2 group in which SD (1.2 g/200 g rat)was given via stomach tube 3 d before intestinal I/R. A strip of small intestine was taken from distal end of ileum for electron microscopic examination. The two-dimensional structural parameters and three-dimensional structural parameters of mitochondria were calculated. RESULTS: (1)Morphological changes of small intestine: In control group, epithelial cells were orderly arranged, with normal mitochondria and intestinal villi. In model group, the gaps between epithelial cells widened. There were a lot of apoptotic cells. Microvilli were short and swelled. Mitochondria were swelled obviously with broken ridges. Endoplasmatic reticulum was severely dilated. In SND1 and SND2 groups, microvilli and epithelial cells were orderly arranged relatively, mitochondria was slightly swelled. (2) Structural parameters of mitochondria: In model group, there were the least mitochondria and the swelling of mitochondria was severe. In SND1 and SND2 groups, the mitochondria was more than that of model group and the swelling were slight. CONCLUSION: Sini decoction can protect small intestine from ischemia-reperfusion injury without dose-dependent effect. 相似文献