紫花苜蓿受冻害后促进根颈枝条再生方法的研究     

张宝田  穆春生  李志坚  周道玮 《中国草地学报》2003,25(5):48-51

对蔚县苜蓿根颈冻害后切除冻伤部分处理的比较研究结果表明,处理区的根颈苗数、地上高度、地上生物量、分蘖丛数是对照区的1.6～2倍;处理区苜蓿的各个数量指标的b值大于对照处理苜蓿b值,说明除冻害根颈后苜蓿的生长比不除冻害根颈部分的苜蓿生长更为合理。通过除去苜蓿冻害部位,防止根系继续腐烂,保护了未冻害部分,增加了苜蓿发芽的有效部位。同时,使根系与地面的距离变短或露出地面,增加光照强度,提高地温,促使未冻害苜蓿根系发芽,返青提前增加产量。  相似文献   

实广义正定矩阵的若干性质     

袁德正 《内蒙古农业大学学报(自然科学版)》1990,(2)

矩阵∈AR~(n×n)称为实广义正定矩阵,如果对任意非零向量 X∈R~n,有XTAX>0成立。本文讨论了矩阵的kronecker积。Hadamard积和矩阵乘积的正定性,给出相应一些性质。  相似文献   

Bovine foetal sex determination—Different DNA extraction and amplification approaches for efficient livestock production          下载免费PDF全文

M Ristanic  Lj Stanisic  M Maletic  U Glavinic  V Draskovic  N Aleksic  Z Stanimirovic 《Reproduction in domestic animals》2018,53(4):947-954

Foetal sex determination using polymerase chain reaction (PCR) in mammals is based on the amplification of gender‐specific foetal DNA sequences circulating in maternal blood. The bovine synepitheliochorial placenta does not allow a direct contact between the trophoblast and the maternal blood, resulting in difficult passage of foetal DNA and, consequently, its very small amounts in maternal bloodstream. Circulating cell‐free foetal DNA (ccffDNA) encompasses short nucleotide fragments (300–600 bp) in maternal circulation. The aim of this study was to assess this non‐invasive method in accurate prenatal sexing in early and late gestational periods in comparison with ultrasound diagnostics. As various DNA isolation and amplification methods were tested, their success in obtaining reliable results was evaluated. Two groups were tested, each consisting of 20 pregnant cows. Blood of a bull and a non‐pregnant heifer was the controls. Extraction of foetal DNA was accomplished by three different methods: using tubes with silicone membranes, a single‐tube extraction without silicone membranes and phenol–chloroform extraction. Following each extraction method, foetal DNA was amplified using PCR and real‐time PCR with both bAML and TSPY primers in a separate reaction. Positive results were obtained only after amplification of foetal DNA extracted with a single‐tube extraction kit. In comparison with ultrasound examination results and foetal gender recorded at birth, the sensitivity of the PCR test was 90% in Group I, but the technique failed to detect male foetuses in Group II. The real‐time PCR test sensitivity in Group I was 90% and in Group II 91.6%.  相似文献   

Endocannabinoids as endometrial inflammatory markers in lactating Holstein cows          下载免费PDF全文

R Bonsale  R Seyed Sharifi  E Dirandeh  N Hedayat  A Mojtahedin  M Ghorbanalinia  A Abolghasemi 《Reproduction in domestic animals》2018,53(3):769-775

The objective of this study was to consider endocannabinoid system as inflammatory markers in bovine endometrium to better understand the role of this system in regulating many of the functions that are related to inflammatory condition. At day 26 post‐partum, fourteen cows were divided into two groups depending on the inflammatory condition: 1‐ subclinical endometritis (n = 7, with purulent or mucopurulent uterine discharge detectable in the vagina) and 2‐ healthy (n = 7, No (muco)) purulent discharge. Blood samples were collected at 26 and 30 days relative to calving to determine plasma tumour necrosis factor (TNF) and lipopolysaccharide‐binding protein (LBP) concentrations; moreover, uterine biopsy was carried out on day 26 post‐partum to measure mRNA abundance of TNF, interleukin‐1B (IL1B), interleukin‐6 (IL‐6), C‐X‐C motif chemokine ligand 8 (CXCL8), endocannabinoid receptor (CNR2), N‐acyl phosphatidylethanolamine phospholipase D (NAPEPLD), fatty acid amide hydrolase (FAAH), N‐acylethanolamine acid amidase (NAAA) and monoglyceride lipase (MGLL) by real‐time PCR. Results showed mean plasma concentrations of TNF and LBP were lower in healthy cows compared to subclinical endometritis cows (p < .05). Relative mRNA expression for NAAA and FAAH was decreased (p < .05), and relative mRNA expression for CNR2 and NAPEPLD increased in cows with subclinical endometritis compared to healthy cows. In conclusion, relative mRNA expression of TNF, IL1B and CXCL8 and plasma concentration of LBP increased during inflammatory condition along with decreased endocannabinoids hydrolyzing enzyme (NAAA and FAAH), increased enzymes that synthesize endocannabinoids (NAPEPLD) and relative gene expression of the endocannabinoid receptor; together, these contribute to increased endocannabinoids levels during inflammation. Overall, we provide evidence that endocannabinoid system is altered in endometrium tissue during inflammation through increased mRNA expression of CNR2 and synthesis enzyme and decreased mRNA expression of hydrolyzing enzymes interfere with pro‐cytokine production and signalling, which may interfere with the onset and progression of inflammation.  相似文献   

房地产销售系统的软件设计与实现   总被引：1，自引：0，他引：1  

李雯瑞 《信阳农业高等专科学校学报》2011,21(1):134-135,138

房地产业的飞速发展,给房地产销售管理带来了很多新的问题和任务,传统的管理模式已经不能适应现代房地产销售管理的需要,亟待建立一套房地产销售管理信息系统,以满足房地产管理信息化、科学化、现代化的需求。  相似文献   

我国开征房产税的可行性研究     

魏勇 《云南农业大学学报(自然科学版)》2011,5(3):59-63

随着房价的居高不下，房产税的征收在近期已成为热门话题。通过研究房产税开征的情况，追溯房产税的历史起源，考察征收房产税的原因，以及分析在现时的中国征收房产税的特殊情况，并综合运用比较法、例证法等各种方法，以期在众说纷纭中，得到一个清醒的认识，并最终说明房产税征收的时机并不成熟，现在的征收热还只是国家的造势之举  相似文献   

基于无线传感器网络的水质监测系统研究     

黄亦磊  郁炜  叶国聪  周凯杰  赵金鑫 《湖南农机》2015,(3):51+53

针对国内外水质环境研究中水质样品异地监测分析存在的巨大缺陷,研究了水质自动监测系统。文章介绍了基于无线通讯传感器网络的简易水质自动监测系统,实现水质数据自动采集和实时上传,可以实现对钱塘江源头水质（衢州水质）进行更好的监测及保护。  相似文献   

Assessing the within-herd prevalence of Coxiella burnetii milk-shedder cows using a real-time PCR applied to bulk tank milk     

Guatteo R  Beaudeau F  Joly A  Seegers H 《Zoonoses and public health》2007,54(5):191-194

Thirty-seven bulk tank milk (BTM) and individual milk samples of all contributing cows were tested for Coxiella burnetii detection by a real-time PCR assay and used to assess the relationship between the BTM PCR-response and (i) the within-herd prevalence of milk-shedder cows and (ii) the proportion of heavy milk-shedder cows. The within-herd prevalence of milk-shedder cows (i) was found to be significantly higher in herds with a positive BTM and (ii) increased significantly with the estimated titre in Coxiella burnetii obtained in positive BTM. The proportion of heavy milk-shedder cows among the milk-shedder cows increased significantly with an increased estimated titre in Coxiella burnetii in positive BTM. Therefore, a real-time PCR assay applied to BTM samples collected repeatedly over time appears to be a valuable tool to assess on a larger scale the status of herds towards Coxiella shedding, and to evaluate the efficiency of control actions aimed at controlling and/or preventing Coxiella shedding in dairy herds.  相似文献   

Research on Relationship between the Level of Urbanization and the Growth of Real Estate Investment          下载免费PDF全文

XIANG Wei -min  LI Jiao 《保鲜与加工》2007,(1):106-109

The population proportion index is usually introduced to measure the level of urbanization, which is an important index to the national economy. The fast development of urbanization would inevitably cause the increase of the investment in real estate industry. Introducing the Granger Test of Causality analytical method, in this paper the relationship between the level of urbanization and the investment in real estate industry from 1986 to 2003 in China is analyzed. It reveals that both of them are on the stage of constant up - rising, and the correlation index of 0. 205 shows that the relationship between them is a mutual promoting causality.  相似文献   

Study on Institutional Defect of Mortgage-Backed Securitization and Countermeasures in China     

REN Hong  XIA Gang  CHEN Lei 《保鲜与加工》2007,(2):122-127

Mortgage-backed securities(MBS),originated in the U.S.and introduced into China in 2005,is a vehicle for real estate finance.This paper describes their history,mechanism,development,theory and experiences in the USA and other countries.In China,the real estate finance is partitioned into four stages: first,non-finance stage(1949-1977);second,sprouting stage(1978-1997);third,inceptive stage(1998-2004) and the fourth,developing stage(2005-),and it is distinguished from the finance of Real Estate Development firm.The differences between them are in following aspects: object,objective,mode and risk.This paper analyzes the institutional defects in view of the policy environment,the primary market of MBS,the secondary market of MBS,and offers countermeasures: first,setting up National Housing Mortgage Management Institution,second,enlarging the service scope of housing mortgage-backed loan,third,differential loaning,fourth,regularly issuing the pricing of housing construction;fifth,normalizing the institutional investors.  相似文献