狗舌草提取物诱导淋巴细胞性白血病L1210细胞分化的研究     

陈进军  王建华 《中国兽医科技》2005,35(11):892-894

以单猪屎豆碱（MO）、槲皮素（QU）和环磷酰胺（CY）为参照，观察了狗舌草600mL／L乙醇提取物（EX）对淋巴细胞性白血病L1210细胞体外试验的形态变化；利用流式细胞术，从DNA分子水平上检查了EX对L1210细胞各周期相的影响，探讨EX对L1210细胞的分化机理。结果发现，EX能够使L1210细胞向淋巴细胞方向发展；经EX作用24h后，L1210细胞G0＋G1期的百分比较对照组明显升高。提示EX对L1210细胞增殖的抑制作用可能是由于G1期的阻滞所致。  相似文献   

Increased expression of CD40 ligand by peripheral blood mononuclear cells in patients with systemic lupus erythematosus     

LI Yu-jie  LI You-ji  YE Ren-gao  KONG Qing-yu  DONG Xiu-qing  YU Xue-qing 《园艺学报》2003,19(4):514-517

AIM:To investigate expression and function of CD40 ligand by peripheral blood mononuclear cells (PBMCs) in patients with systemic lupus erythematosus (SLE).METHODS:Expression of CD40 ligand by PBMCs in patients with SLE and control were examined by flow cytometric analysis before and after stimulated by phytohemagglutinin(PHA)and depressed by Dexamethasone(Dex). The correlation between expression of CD40 ligand and SLE activity index(SLEDAI) was analysed in patients with SLE.RESULTS:The expression of CD40 ligand by PBMCs in patients with active SLE was higher than that in patients with inactive SLE and control. Though the expression of CD40 ligang by PBMCs could be stimulated by PHA in three groups, it was the highest in patients with active SLE. Dex depressed the expression of CD40 ligand by PBMCs significantly in patients with SLE, but not in control. There was high positive correlation between expression of CD40 ligand and SLEDAI in patients with active and inactive SLE.CONCLUSION:Increased expression of CD40L by PBMCs in patients with SLE may play an important role in pathogenesis of SLE.  相似文献   

Effect of cocaine on caspase-3 in myocardiac cells of male rats in different age     

WANG Hong-ju  HUANG Yuan-wei  YANG Guo-sheng  ZHANG Lei 《园艺学报》2003,19(9):1193-1196

AIM: To study the effect of cocaine on caspase-3 in myocardiac cells of male rats in different age. METHODS: Three-week-old(n=16), six-week-old(n=16) and twelve-week-old (n=16) male Sprague-Dawley rats were all divided into control groups and experiment groups randomly, each group had eight animals, experiment groups were given cocaine hydrochloride (15 mg·kg^-1 body weight) subcutaneously daily for four weeks. The ratio of heart weight to body weight (HW/BW, mg/g) were measured. DNA fragmentation of myocardia cells was determined by gel electrophoresis, and caspase-3 activity in myocardia cells was tested by flow cytometry (FCM). RESULTS: In three experiment groups, the DNA isolated from myocardial cells displayed clear ladder pattern. The HW/BW and the caspase-3 activity were increased significantly than those of control groups (P<0.01). CONCLUSIONS: Cocaine induced apoptosis in rat myocardial cells. The increase in caspase-3 activity may be the one of important pathways related to the induction of apoptosis.  相似文献   

The expression of IFN-γ and IL-4 on T lymphocytes that infiltrate in nasal polyps     

CHEN Rong  SHI Jian-bo  XU Geng  DI Jing-fang  ZENG Shan  ZENG Yao-ying 《园艺学报》2003,19(12):1623-1626

AIM:To investigate the expression of Th1-typed cytokine IFN-γ and Th2-typed cytokine IL-4 on T lymphocytes that infiltrate in nasal polyps for searching the pathogenesis of nasal polyps. METHODS:Nasal polyps tissue samples and peripheral blood were obtained from 21 patients. Normal human inferior turbinate mucosa and peripheral blood were obtained as well. Flow cytometry was adopted to detect the expression of IFN-γ and IL-4 of T lymphocytes. RESULTS: Th cytokines were rarely detected in inferior turbinate from normal human. Nasal polyps tissue consisted of abundant T lymphocytes. The expression of IL-4 and IFN-γ increased in peripheral blood from patients compared with normal human (P<0.05). The expression of IL-4 increased but the expression of IFN-γ decreased in nasal polyps compared with that of peripheral blood from the same patient (P＜0.05). CONCLUSION:There were generous of T lymphocytes infiltrating in nasal polyps. There was abnormal immune status in the local nasal mucosa from the patients, and the predomination of Th cytokine secretion changed compared with peripheral blood from the same patients, which resulted in the change of microenvironment of nasal mucosa and possibly close related to the formation of nasal polyps.  相似文献   

Relationship between expression of somatostatin and DNA ploidy in breast cancer     

SUN Xin  GUAN Wen-zeng  LI Nai-qing 《园艺学报》2002,18(11):1394-1396

AIM: To investigate the relationship between somatostatin and the pathologic type, estrogen receptor,DNA ploidy of nuclei in tumor cells of breast cancer.METHODS: 67 cases of primary breast cancer and 25 cases of benign breast tumor were examined by immunohistochemical stretomyces avidin peroxidase method. 26 cases of breast cancer selected at random were analyzed by flow cytometry.RESULTS: Somatostatin expressed significantly higher in low malignant breast cancer than that in high malignant breast cancer (P<0.05). Most of cancers with positive staining of somatostatin were diploidy,most of cancers with negative staining were aneuploidy,there had significant difference between two groups (P<0.05).CONCLUSION: Somatostatin may delay the progress of breast cancer,and somatostatin levels in cancer tissues may become a useful indicator for assessing prognosis of patients with breast cancer.  相似文献   

两种血清抗性检测方法的比较分析     

徐旺烨  樊琛  李一经 《湖北农业科学》2016,(21):5571-5573

采用流式细胞术法对大肠杆菌的血清抗性进行检测,并与传统涂板法相比较,探讨流式细胞术在血清抗性检测方法中的应用。结果表明,两者呈正相关关系(P=0.0340.05),流式细胞术法可以代替涂板法对大肠杆菌的血清抗性进行检测。该方法可以减少由于涂板不均匀、人工计数等造成的误差,且易操作、计算少、耗时少,数据更准确。  相似文献   

The Fluorescent Characterization and Analysis of Two Brucella Attenuated Vaccine Infecting Mouse Macrophagocyte     

HU Meng-wei  LIU Peng-tao  YAN Guo  GAO Jian-feng 《中国畜牧兽医》2016,43(8):1944-1950

The experiment was conducted to discuss the difference of binding time of green fluorescent protein B.melitensis M5 (GFP-M5) and B.abortus S19 (GFP-S19) infecting the mouse macrophagocyte (RAW264.7),lysosome,endoplasmic reticulum and golgi body in the initial stage and compare the binding rate of GFP-M5,GFP-S19 with organelle in different timeline,respectively,by confocal laser scanning microscope (CLSM) and flow cytometry.The result showed that GFP-M5 and GFP-S19 were successfully constructed.The intracellular survival ability of Brucella M5,Brucella S19,GFP-M5 and GFP-S19 were not obvisouly affected after infecting RAW264.7.GFP-M5 and GFP-S19 could enter the macrophagocyte in 30 mins,and in 2 h the Brucella could reach lysosome,endoplasmic reticulum and golgi body.In addition,the binding time for two attenuated vaccine did not show differences in 1,2,3 and 4 h.The content of GFP⁺ cell produced by RAW264.7 infected by GFP-M5 and GFP-S19 did not show significant differences (P>0.05).Therefore,the two strains did not have significant differences in the invasion ability in the initial stage of infecting host cell.  相似文献   

野生老芒麦种质资源鉴定与基部叶鞘绒毛变异分析     

李明峰  李欣瑞  李英主  陈丽丽  王艳  李春艳  常丹  雷雄  闫利军  游明鸿  季晓菲  张昌兵  吴婍  苟文龙  李达旭  陈仕勇  白史且  鄢家俊 《草地学报》2023,31(4):1026-1035

为更好地利用老芒麦(Elymus sibiricus L.)野生资源,本研究从我国青藏高原、西北、华北、东北地区以及国外部分地区共采集了1 723份野生披碱草属种质资源,通过表型特征观测和流式细胞仪检测,共鉴定出了990份野生老芒麦种质,246份垂穗披碱草种质,并测得老芒麦的DNA含量在5.86～7.30 Gb之间,平均DNA含量为6.66 Gb;垂穗披碱草材料的DNA含量在9.50～10.36 Gb之间,平均DNA含量为9.97 Gb。研究结果显示,在西北、华北以及东北地区的部分野生老芒麦材料在苗期基部叶鞘出现了绒毛,分析发现该表型出现的概率与经度和纬度呈极显著正相关关系(P<0.01),与海拔、年年均气温以及年平均降雨量呈极显著负相关关系(P<0.01)。表明老芒麦苗期基部叶鞘绒毛的有无与环境因子之间存在着必然的联系,可能是老芒麦种质应对不同环境类型的适应性机制之一。本研究为老芒麦的形态鉴定提供了新的依据,为老芒麦种质资源挖掘和育种应用提供了材料基础。  相似文献   

Evaluation of a novel moving threshold gating strategy for assessment of reticulated platelets in dogs using the ADVIA 2120 analyzer     

Anja Kuhn  Kristina Weiler  Graham Gibbs  Gottfried Prechtl  Natali Bauer  Andreas Moritz 《Veterinary clinical pathology / American Society for Veterinary Clinical Pathology》2023,52(1):11-21

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高雷诺数对无量纲参数的影响     

赵新顺  舒歌群  刘书亮  户东 《拖拉机与农用运输车》2006,33(1):44-46,49

研究了高雷诺数对气体滚流强度和流量系数的影响。试验表明,气门升程和气道压差小时,难以形成充分发展的湍流,随着气门升程的增大,无量纲滚流强度和流量系数呈上升趋势,并且波动较大;充分发展时,可压缩气体的滚流强度和流量系数恒定不变,不可压缩气体的滚流强度比真实值偏大,而流量系数比真实值偏小,误差大小只与压力比有关,并随着压力比的增大而增大。  相似文献