青霉素V钾滴丸的研制     

肖国君  陈力  易木林  陈波 《中国兽药杂志》2003,37(6):17-19

为减少青霉素V钾的水解并提高其疗效，用聚乙二醇400和聚乙二醇6000为基质制备青霉素V钾滴丸。体外抑菌试验表明，青霉素v钾滴丸抑菌作用略强于人药片剂。  相似文献   

The possibility of using oxidation–reduction potential to evaluate fish freshness     

Tri Winarni Agustini  Masaki Suzuki  Toru Suzuki  Tomoaki Hagiwara  Shoichi Okouchi  Rikuo Takai 《Fisheries Science》2001,67(3):547-549

  相似文献   

羊草草原枯枝落叶积累的研究：枯枝落叶积累对草原生态环境及生产力的作用   总被引：5，自引：0，他引：5  

郭继勋  祝廷成 《草业科学》1994,11(6):12-16

枯枝落叶积累可改变原生态环境，地表温度、土壤ＰＨ值积累量呈线性负相关，蒸发量、土壤容重与积累量呈指数负相关，土壤水分、土壤孔隙度与积累量呈线性正相关，枯枝落叶积累可为植物生长创造良好的生境。土壤中主要营养物质随着枯枝落叶积累均有明显提高当积累量达６００ｇ／ｍ＾２时，有机质、Ｎ、Ｐ、Ｋ含量分别比无枯枝落叶层提高１．８倍、８８．２％、２．１５倍和３７％。枯枝落叶积累对草原生产力有促进作用，在一定范围内  相似文献   

夏大豆亩产262.1kg生理指标研究   总被引：4，自引：1，他引：3  

卢增辉  赵俊杰 《大豆科学》1994,13(3):185-192

研究了夏大豆中油－８９Ｄ亩产２６２．１ｋｇ各项生理指标：叶面积系数，开花期、结荚期、鼓粒期分别为３．９８、５．８０、４．９１；总光合势１８．２万ｍ＾２·日，平均净光合生产率４．８１ｇ／ｍ＾２·日，氮磷总积累量分别为２２．２８ｋｇ和３．１３ｋｇ，每生产１００ｋｇ籽粒需吸收氮８．５０ｋｇ，磷１．１９ｋｇ，总干物质积累８７６．４７ｋｇ，粒茎比０．５１，经济系数０．３０。  相似文献   

表达大肠杆菌K88ac-ST1-LTB融合蛋白工程菌株的免疫原性研究   总被引：2，自引：0，他引：2  

许崇波  卫广森  王文成  王卓 《中国预防兽医学报》2003,25(1):9-12

已构建的能表达大肠杆菌K88ac-ST1-LTB融合蛋白的工程菌株BL21（DE3）（pXKST3LT5)及其表达产物经动物试验证实没有毒性反应。用从IPTG诱导的工程菌中提取的包涵体或经甲醛灭活的工程菌制成抗原，免疫小鼠，结果免疫小鼠至少能抵抗2MLD的大肠杆菌强毒株C83902（K88ac,ST^ ,L^ )的攻击，用提取的包涵体免疫家兔后，采集的血清能够中和天然ST1的毒性，这表明构建的工程菌株BL21（DE3）（pXKST3LT5)可以作为预防幼畜大肠杆菌性腹泻基因工程菌苗的候选株。  相似文献   

The effect of some Frescon analogs on the aquatic snail,Lymnaea stagnalis: II. Correlation of neurotoxicity to Molluscicidal effectiveness     

B.L. Brezden  D.R. Gardner 《Pesticide biochemistry and physiology》1980,13(2):178-188

Although the molluscicide Frescon is a strong neurotoxin to the Lymnaea stagnalis central nervous system in vitro, it is probable that the exposure of the whole animal to this molluscicide fails to result in central nervous system abnormalities: Frescon does not appear to reach the brain in sufficient quantity to disrupt its normal activity. However, only those Frescon analogs found to be neurotoxic were molluscicidal, suggesting some related mode, if not site, of action. Frescon and its analogs may act by affecting excitable tissues other than the nervous system (e.g., the snail musculature) by altering certain functional and/or structural membrane properties.  相似文献   

Antisense drug targeting with VEGF mRNA designed by computer aid and inhibitory effects on K562 cell line in vitro     

FEI Jia  ZHANG Huan 《园艺学报》2003,19(5):645-648

AIM:The effective antisense sequences targeted VEGF mRNA with computer software would be screened and designed, and effect of them on growth K562 cells and protein expression of VEGF were studied with experiments.METHODS:Seven antisense sequences were selected and synthesized, which consisted of 18-20 deoxynucleotide acid and were modified with phosphorothioate, according to principle of low free energy of overall △G₃₇ Overall. Cell growth was assayed by trypan blue dye exclusion assay and level of VEGF protien in the media was determined by ELISA.RESULTS:Six of seven sequences were capable of inhibing growth of K562 cells and downregulating the VEGF protein expression significantly, compared with Scrambed control group. It was found that there was a close correlation between low level of overall △G₃₇ and antisense effectiveness (r=0.887,P<0.01).CONCLUSION:VEGF mRNA antisense oliogdeoxynucleotides, which were designed by computer software of RNAstructure, were able to inhibit growth of K562 and its protein expression. The VEGF mRNA may be new target attached by drugs. At same time, the computer aided design is useful methods to obtain the effective antisense.  相似文献   

Protective effect of onychin on the human umbilical vein endothelial cells injured by menadione     

GUO Yu  ZHU Bing-yang  YAN Feng-xiang  HUANG Hong-lin  LIAO Duan-fang 《园艺学报》2003,19(1):10-13

AIM: To investigate the protective effect of onychin on the endothelial cells injured by oxidative stress. METHODS: The injured model was established by endothelial cells treated with menadione. The growth inhibitory rate of endothelial cell was determined by MTT assay; NO₂^-／NO₃^- concentration in the medium was determined by nitrate reductase assay; eNOS and caveolin-1 protein levels were determined by Western blot. RESULTS: Onychin significantly decreased the growth inhibitory rate of endothelial cells injured by menadione, increased NO₂^-／NO₃^- concentration in the medium and eNOS activity and up-regulated caveolin-1 expression. CONCLUSION: Onychin possesses a protective effect against endothelial cell injury induced by menadione via caveolin-1/eNOS pathway.  相似文献   

Bcl-2 antisense oligodeoxynucleotide increases the sensitivity of HL60 and K562 cells to daunorubicin     

LEI Xiao-yong  ZHANG Huan  HE Dong-mei 《园艺学报》2003,19(2):194-197

AIM:To investigate whether the bcl-2 antisense oligonucleotide increases the sensitivity of HL60 and K562 cell lines to daunorubicin.METHODS:IC50 for HL60 and K562 was determined with MTT method, the expression levels of Bcl-2 protein were assayed by immunofluorescence using fluoresce isothiocyanate labeling. In addition, apoptosis was detected by morphological observation and flow cytometric analysis of DNA fragmentation.RESULTS:It was found that the two oligonucleotides directed against the coding region and the translation initiation of bcl-2 mRNA, combined respectively with daunorubicin, inhibited expression of bcl-2 protein, increased apoptosis in HL60 and K562 cells, and decreased IC50 of daunorubicin significantly (P＜0.05). Compared to the antisense oligonucleotide directed against the translation initiation of bcl-2 mRNA, the antisense oligonucleotide directed against the coding region showed stronger effects in the aspects of increasing the sensitivity of HL60 cells to daunorubicin (P＜0.05).CONCLUSIONS:These two antisense sequences in the translation initiation and the coding region of bcl-2 mRNA increased the sensitivity of HL60 and K562 cell lines to daunorubicin in a sequence-specific manner.  相似文献   

Effect of Liucha extract on tumor K562 cell growth     

LIU Xin  PAN Xing-bin  LI Xing-yu 《园艺学报》2003,19(2):253-255

AIM:To investigate the effects of Liucha extract on the growth of tumor cells in vitro and its possible mechanism.METHODS:The capability of colony forming of human leukemia K562 cell in vitro and the cells metabolism were studied by semi-solid agar culture and MTT staining. Then , the changes in morphology in the tumor cells were examined using electronic microscope.RESULTS:Semi-solid agar culture and MTT colorimetric analysis showed that Liucha extrats could significantly inhibit the growth of the tumor cells and their capability of colony forming. Also,under the electronic microscope,it was found that the tumor K562 cell had a narrower perinuclear space,condensation of chromatin and an enlarged mitochondria , in which the cristase disappeared.CONCLUSION:The extract from Liucha possesses an inhibitory effect on K562 cell growth in vitro through affecting the metabolism of the tumor cells.  相似文献