排序方式： 共有94条查询结果，搜索用时 31 毫秒
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Vaidhyanathan Bhuvaneswari Balaiya Velmurugan Seetharaman Balasenthil Chinnamanoor R. Ramachandran Siddavaram Nagini 《Fitoterapia》2001,72(8)
The chemopreventive efficacy of lycopene on 7,12-dimethylbenz[a]anthracene (DMBA)-induced hamster buccal pouch (HBP) carcinogenesis was examined using lipid peroxidation, reduced glutathione (GSH), glutathione peroxidase (GPx), glutathione S-transferase (GST) and glutathione reductase (GR) as biomarkers of chemoprevention. Twenty four male Syrian hamsters were divided into four groups of six animals each. The right buccal pouches of the animals in group 1 were painted with a 0.5% solution of DMBA in liquid paraffin three times a week. The animals in group 2 were painted with DMBA as in group 1 and in addition received 2.5 mg/kg body weight lycopene orally three times a week on days alternate to DMBA application. Group 3 animals received lycopene as in group 2. Animals in group 4 received neither DMBA nor lycopene and served as control. The hamsters were killed after an experimental period of 14 weeks. Biochemical measurements were carried out in tumour and normal tissues. All hamsters painted with DMBA alone for 14 weeks developed well-differentiated squamous cell carcinomas. Diminished lipid peroxidation in the oral tumour tissue was accompanied by a significant increase in the levels of GSH, GPx, GST and GR. Administration of lycopene significantly suppressed DMBA-induced oral carcinogenesis as revealed by the absence of carcinomas. The results of the present study suggest that lycopene may exert its chemopreventive effects by modulating lipid peroxidation and enhancing the activities of the enzymes in the glutathione redox cycle. 相似文献
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[目的]介绍了红酵母分离纯化的方法,菌株系统发育分析及发酵产物番茄红素的测定。[方法]从土壤及落花样品中分离纯化出7株红酵母,对其进行18S rDNA序列分析,并用高效液相色谱法对7株野生菌株的胞内色素产物进行了初步分析鉴定。[结果]18SrDNA序列分析可初步鉴定这7株菌分别属于红冬孢酵母菌属和红酵母属。用高效液相色谱测定7株菌种胞内抽提物中的番茄红素含量,发现仅有菌株06944的胞内抽提物中含有番茄红素。将菌株06944发酵产番茄红素的HPLC图谱与番茄红素标准品的对比,可初步鉴定所筛出的菌株06944产番茄红素,产量为2.96μg/ml。[结论]菌株06944为红冬孢酵母属,无致病性,可直接作为工业生产菌种。 相似文献
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番茄红素油树脂混合环糊精包合研究 总被引：2，自引：0，他引：2
[目的]研究混合环糊精(β-环糊精、羟丙基-β-环糊精)对番茄红素油树脂的包合性能并验证其实用性。[方法]紫外法测定番茄红素与β-环糊精、羟丙基-β-环糊精、混合环糊精的包合常数;采用研磨法制备包合物并测定溶解度,考察其稳定性。[结果]番茄红素油树脂与混合环糊精包合常数为758~1 247 L/mol,大于与β-环糊精的416~608 L/mol;高性价比混合环糊精的包合物,其溶解度提高近70倍且稳定性提高。[结论]番茄红素油树脂/混合环糊精包合物实用性大大增强。 相似文献
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Lycopene β-cyclase (LCYb) and lycopene ?-cyclase (LCYe) are believed to be crucial genes to lycopene cyclization and downstream carotenoid accumulation. To illuminate the gene expression profiles and their regulation on downstream carotenoids, expression profiles of these two genes and contents of several corresponding carotenoids were determined by real-time PCR and HPLC, respectively, in relative shorter intervals during breaker and maturation stage of Guoqing No. 1 satsuma mandarin (Citrus unshiu Marcow) and Cara Cara navel orange (Citrus sinensis Osbeck). Data showed that the expression profiles of these two cyclase genes and their relative expression levels between the peels and the pulps at the same stage all varied with cultivars. But in general, accumulation of lutein and β-carotene experienced a similar tendency in both cultivars and were up-regulated by expression of LCYb and LCYe in spite of poor correlation in the pulps. Meanwhile, lycopene abundance was negatively correlated with the expression of LCYe as expected, but hardly correlated with LCYb in the pulps of Cara Cara navel orange, indicating that LCYe might exert a more prominent influence on lycopene cyclization and downstream carotenoids formation. Results of carotenoids accumulation suggest that the biosynthetic process of carotenoids would shift from upstream β, ?- or β, β-carotenoids such as lutein and β-carotene to downstream β, β-carotenoids during fruit development. 相似文献
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施用钾肥对西瓜番茄红素的影响研究 总被引：1，自引：0，他引：1
比较了0,150,300,450和600mg/L等5个钾水平下成熟西瓜果实中番茄红素含量的差异。研究结果表明,随着施钾水平的提高,番茄红素的含量线性上升,它们分别为2.94,3.65,4.19,4.62和5.31mg/100g,且R2=0.9935。 相似文献
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番茄红素抗氧化活性的研究 总被引：7，自引：0，他引：7
用化学发光法研究了纯度≥99%的番茄红素(Lycopene)对超氧阴离子、羟基自由基、DPPH.和由AAPH引发月笕草油产生的脂质过氧化自由基的清除作用,结果表明,Lyp对4种自由基均有较强的清除作用,并且具有显著的剂量-效应关系。EC50分别是11.95、13.34、52.65、13.38μg/m1。Lyp对超氧阴离子、羟基自由基的清除速度较快,反应在6 s内即可达到或接近最大值;对DPPH.、脂质过氧化自由基的清除率达到最大值所需的时间为12~18 s。 相似文献
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硒钴配施对番茄产量、番茄红素及硒、钴含量的影响 总被引：1，自引：0，他引：1
采用盆栽试验研究了硒钴配施对番茄产量、番茄红素、番茄果实中硒、钴含量以及土壤中有效硒、钴含量的影响,结果表明,单施硒对番茄产量无明显影响;单一施钴处理时,番茄产量随施钴量的增加而增加;Co2(16 mg/kg)与Se2(0.6 mg/kg)或Se3(3 mg/kg)配施可显著提高番茄的产量,与对照相比,分别提高了14.1%和15.9%。单一施硒和单一施钴以及硒钴配施条件下均可提高番茄中番茄红素的含量。单一施硒和硒钴配施均可明显地提高番茄中硒和土壤中有效硒的含量。单一施钴以及硒钴配施可明显提高番茄中和土壤中有效钴的含量。 相似文献
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番茄红素环化酶基因片段克隆及反义表达载体构建 总被引：1，自引：1，他引：0
根据已知的番茄红素环化酶基因,即13环化酶基因和s环化酶基因的保守序列设计特异性引物。提取番茄叶片的RNA,经RT—PCR反应,扩增出723bp和569bp的目的片段,将它们分别连接到pEASY—T1 Cloning Vector上,测序鉴定其正确性。克隆到载体上的13环化酶基因用BamHI和SmaI双酶切,将基因反向插入PROK2表达载体上,构建PROK2-LYCb反义表达载体。同样连接到克隆载体上的s环化酶基因用BamHI和XbaI双酶切,将基因反向插入PROK2表达载体上,构建PROK2-LYCe反义表达载体。 相似文献
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红肉脐橙树体不同部位果肉主要色素和糖含量的变化及其相关性 总被引：3，自引：0，他引：3
研究了红肉脐橙树体不同部位果实果肉中主要色素和糖含量的动态变化,并对其相关性进行了分析.结果表明:内膛和外围果实的番茄红素和β-胡萝卜素含量变化趋势仅在果实着色后期和成熟期(11月10日至12月25日)有所不同,果实成熟时内膛果实的番茄红素含量极显著低于对照,但β-胡萝卜素含量极显著高于对照;内膛与外围果实的各种糖含量的变化趋势基本一致,但果实着色后期和成熟期(11月10日至12月25日)内膛果实的各种糖含量都显著或极显著低于对照(12月25日的蔗糖含量除外);外围果实的番茄红素、β-胡萝卜素、葡萄糖、果糖、蔗糖和总糖两两之间呈显著或极显著正相关关系,内膛果实的β-胡萝卜素、葡萄糖、果糖、蔗糖和总糖两两之间也呈显著或极显著正相关关系,番茄红素含量与其他指标之间呈正相关关系但不显著. 相似文献