九种根际促生菌最适培养条件初探   总被引：1，自引：3，他引：1  

汤春梅  陈秀蓉  姚拓  龙瑞军 《草原与草坪》2005,(3):27-30

测定了分离自燕麦和小麦根际九株促生菌对温度、pH值、光照及培养方式的需求,结果表明:虽然九株促生菌适应范围较广,即在5~45℃、pH4~9、3种光照条件(12h黑暗12h光照、24h光照和24h黑暗)和3种培养方式(全天振荡、间歇振荡和静止培养)下均能较好生长,但不同菌株的最适培养条件不尽相同。通过对各菌株的最适培养条件进行了测定和分析,为后续确定促生菌的最佳发酵条件和生态适应性评价提供了依据。  相似文献   

Enhancement in Nutrient Accumulation and Growth of Oil Palm Seedlings Caused by PGPR Under Field Nursery Conditions     

《Communications in Soil Science and Plant Analysis》2012,43(15-16):2059-2066

Abstract

Plant growth promoting rhizobacteria (PGPR) (e.g., Azospirillum and Bacillus spp.) have been reported to enhance growth and fix N₂ with several nonleguminous crops. These rhizobacteria have the potential to be applied to oil palm seedlings and, consequently, reduce the cost of nitrogenous fertilizer. The rhizobacteria are also known as a bioenhancer for the ability to increase root growth and enhanced water and nutrient absorption by the host plants. An experiment was carried out in the field nursery station, Federal Land Development Authorities (FELDA), Bukit Mendi, Pahang, Malaysia, to observe the effects of PGPR inoculation on enhanced nutrient accumulation and plant growth (tops and roots) of oil palm seedlings under field nursery conditions. The inoculation process showed positive response in enhancing higher accumulation of nitrogen (N), phosphorus (P), and potassium (K) in the plant tissues, enhanced root dry weight and top growth (dry matter and leaf chlorophyll content) of the host plants under field nursery conditions.  相似文献   

黄瓜根际促生菌的促生效应与防病作用   总被引：3，自引：0，他引：3  

饶毅萍 《长江蔬菜》2009,(14)

对黄瓜植物根际促生菌(PGPR)菌株的分离筛选、分类鉴定以及人们对其促生防病作用的应用和防病机制的研究现状进行了综述,以期为新型PGPR制剂的进一步研制、开发和应用提供参考.  相似文献   

番茄早疫病菌拮抗细菌的筛选及其抑制作用   总被引：2，自引：0，他引：2  

孙广正  侯栋  岳宏忠  赵桂琴  姚拓  柴晓虹  陈龙 《草原与草坪》2015,(1)

为了筛选对番茄早疫病菌具有生防作用的菌株,利用前期研究获得的17株促生菌,采用平板对峙法测定其对病原真菌的拮抗作用及对菌丝生长的抑制作用。结果发现,可有效拮抗番茄早疫病菌的生防菌有2株,其中,菌株FX2的抑菌活性较好,抑制率达到70.52%,菌株LHS11的抑制率为65.34%。菌株FX2和LHS11可通过次生代谢物抑制病原真菌,其中菌株LHS11的发酵液对番茄早疫病菌的抑制率达到65.40%,菌株FX2的发酵液的抑制率为47.26%。菌株FX2和LHS11能使病原菌菌丝发生不同程度扭曲变形。  相似文献   

PGPR对灰枣土壤养分及微生物数量影响的主成分分析     

秦韵婷  李建贵  郭艺鹏  王先奎  韩超  刘隋赟昊 《经济林研究》2015,(3)

为了得到有益于灰枣生长发育的优势菌种,通过田间试验,研究了灰枣根际促生复合菌株P13K7、P13K24和单一功能菌株P13、P15、K7、K24以及对照(CK)7个处理对灰枣根际土壤养分和微生物数量的影响。应用主成分分析法,将8个指标(p H值、有机质含量、碱解氮含量、速效磷含量、速效钾含量、细菌数量、放线菌数量和真菌数量)简化成2个主成分,2个主成分所提供的信息量占全部信息量的90.78%。结果表明:与对照相比,施用PGPR能够改善灰枣土壤养分和提高微生物总量。经过土壤指标的综合分析得出,复合菌株P13K24的应用效果最好。  相似文献   

Characterization of Burkholderia sp. XTB‐5 for Phenol Degradation and Plant Growth Promotion and Its Application in Bioremediation of Contaminated Soil     

《Land Degradation \u0026amp; Development》2017,28(3):1091-1099

A key issue when researching land degradation is the pollution of soils. For bioremediation of contaminated soil, Burkholderia sp. XTB‐5 cells were obtained from soil and grown on mineral salt medium with initial phenol concentrations of 650 mg L⁻¹ and 850 mg L⁻¹, which were found to degrade more than 98% of phenol content in less than 4 days. About 90% of phenol content (with initial concentration of 250 mg kg⁻¹ soil) was removed from soil inoculated with XTB‐5 cells in 6 days. More than 90% of phenol content was removed within 20 days after co‐introduction of XTB‐5 cells and plants to sterilized soil in a greenhouse or to natural soil in field trials. But under the same conditions, individual introduction of plants to sterilized soil in the greenhouse reduced phenol content by about 50% and introduction to natural soil in field trials reduced phenol content by about 38%, suggesting that phytoremediation of phenol is often inefficient and microorganisms can efficiently degrade this pollutant. In addition, strain XTB‐5 was found to solubilize phosphate and produce 1‐Aminocyclopropane‐1‐Carboxylate (ACC) deaminase and siderophore. Strain XTB‐5 promoted plant growth in both phenol‐absent and phenol‐spiked soil under greenhouse and field conditions. Considering that ACC deaminase is beneficial to plant growth under adverse environmental conditions, plant growth promotion by XTB‐5 in phenol‐contaminated soil is not only due to XTB‐5 cell‐degradation of phenol and reduced phytotoxicity but also to production of ACC deaminase. Hence, Burkholderia sp. XTB‐5 presents an attractive microorganism for phytoremediation of contaminated soil and agronomic application. Copyright © 2016 John Wiley & Sons, Ltd.  相似文献   

Effect of Azospirillum brasilense inoculation on rhizobacterial communities analyzed by denaturing gradient gel electrophoresis and automated ribosomal intergenic spacer analysis     

Anat Lerner  Ezekiel Baudoin  Yvan Moenne-Loccoz  Edouard Jurkevitch 《Soil biology & biochemistry》2006,38(6):1212-1218

Nucleic acid-based techniques allow the exploration of microbial communities in the environments such as the rhizosphere. Azospirillumbrasilense, a plant growth promoting rhizobacterium (PGPR), causes morphological changes in the plant root system. These changes in root physiology may indirectly affect the microbial diversity of the rhizosphere. In this study, the changes in the rhizobacterial structure following A. brasilense inoculation of maize (Zea mays) plants was examined by PCR-denaturating gradient gel electrophoresis (DGGE) and automated ribosomal intergenic spacer analysis (ARISA), using two universal primers sets for the 16S rRNA gene, and an intergenic 16S-23S rDNA primer set, respectively. Similar results were obtained when using either ARISA or DGGE performed with these different primer sets, and analyzed by different statistical methods: no prominent effect of A. brasilense inoculation was observed on the bacterial communities of plant roots grown in two different soils and in different growth systems. In contrast, plant age caused significant shifts in the bacterial populations.  相似文献   

水稻施用植物生长促进菌效果初探     

薛成  张金志  童良军  孔祥森 《黑龙江八一农垦大学学报》2007,19(4):37-40

通过田间试验,对一种植物生长促进菌(PGPR)在水稻上的应用效果作了初步探讨。试验结果表明,PGPR制剂与化肥配合施用对水稻生长发育产生了显著的影响。与单施氮、磷、钾化肥相比,PGPR与化肥处理显著提高了水稻的株高、穗数、穗粒数、千粒重,比单施化肥和不施肥对照分别增产26.0%和53.9%。同时,PGPR处理提高了水稻的根系活力,并对灌浆速率产生了积极的影响。  相似文献   

Inoculation of root microorganisms for sustainable wheat-rice and wheat-black gram rotations in India     

Paul Mäder  Franziska Kaiser 《Soil biology & biochemistry》2011,43(3):609-619

The scarcity of non-renewable resources such as soils and fertilizers and the consequences of climate change can dramatically influence the food security of future generations. Mutualistic root microorganisms such as plant growth-promoting rhizobacteria (PGPR) and arbuscular mycorrhizal fungi (AMF) can improve plant fitness. We tested the growth response of wheat (Triticum aestivum [L.]), rice (Oriza sativa [L.]) and black gram (Vigna mungo [L.], Hepper) to an inoculation of AMF and PGPR alone or in combination over two years at seven locations in a region extending from the Himalayan foothills to the Indo-Gangetic plain. The AMF applied consisted of a consortium of different strains, the PGPR of two fluorescent Pseudomonas strains (Pseudomonas jessenii, R62; Pseudomonas synxantha, R81), derived from wheat rhizosphere from one test region. We found that dual inoculation of wheat with PGPR and AMF increased grain yield by 41% as compared to un-inoculated controls. Yield responses to the inoculants were highest at locations with previously low yields. AMF or PGPR alone augmented wheat grain yield by 29% and 31%, respectively. The bio-inoculants were effective both at Zero and at farmers’ practice fertilization level (70 kg N ha⁻¹, 11 kg P ha⁻¹ in mineral form to wheat crop). Also raw protein (nitrogen × 5.7) and mineral nutrient concentration of wheat grains (phosphorus, potassium, copper, iron, zinc, manganese) were higher after inoculation (+6% to +53%). Phosphorus use efficiency of wheat grains [kg P grain kg⁻¹ P fertilizer] was increased by 95%. AMF and PGPR application also improved soil quality as indicated by increased soil enzyme activities of alkaline and acid phosphatase, urease and dehydrogenase. Effects on rice and black gram yields were far less pronounced over two cropping seasons, suggesting that AMF and PGPR isolated from the target crop were more efficient. We conclude that mutualistic root microorganisms have a high potential for contributing to food security and for improving nutrition status in southern countries, while safeguarding natural resources such as P stocks.  相似文献   

玉米根际细菌中PGPR的筛选及初步鉴定   总被引：6，自引：0，他引：6  

丁延芹  杜秉海 《中国土壤与肥料》2001,(3):41-43

通过离体拮抗试验和检测菌株产生铁载体的情况从玉米根际细菌中筛选出 8株拮抗菌和2 6株铁载体产生菌 ,其中 DC0 1、DC0 9、DC2 2、PY313既具有真菌抗性又能产生铁载体 ,并对部分菌株进行了初步鉴定  相似文献