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This study was undertaken to investigate the feasibility of using Paecilomyces lilacinus NRRL 13 866, chitin, and cellulose amendments, in order to reduce survival and prevent germination of sclerotia from Aspergillus flavus NRRL 6556, NRRL 13 048, and A. parasiticus NRRL 13 005, NRRL 13 539 buried in sandy soil in Georgia and Illinois (April–October, 1990). The number of sclerotia that germinated sporogenically in moist chambers following burial in Illinois was twice that of sclerotia buried in Georgia and varied among the four sclerotium-producing Aspergillus spp. strains. Sclerotium viability, as measured by Aspergillus spp. colony formation on potato dextrose agar, was high (>84%) for all strains and treatments (e.g. amendment, location). Even so, A. parasiticus NRRL 13 539 sclerotia were apparently more susceptible to P. lilacinus colonization than sclerotia from other strains. Treatment with Paecilomyces sp. caused a small but significant reduction in germination among sclerotia buried in Georgia. 相似文献
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油菜菌核病菌菌核室内诱导子囊盘 总被引:2,自引:0,他引:2
油菜菌核病菌(Sclerotinia sclerotiorum L.) 菌核经过6周以上时间的保湿冷冻处理后,放入温室里,诱导萌发和产生子囊盘.初步试验结果表明:在诱导期间,如果不冲洗,一个月后菌核也不会萌发.隔10天或20天,用灭菌水冲洗一次,冲洗后20天左右,菌核萌发产生子囊盘,并能释放出大量的子囊孢子.隔30天后冲洗,25天后菌核才萌发,但产生异常子囊盘,所释放的子囊孢子数也较少,冷冻处理6周至24周对菌核萌发和子囊盘的产生无显著影响.在室内人工接种时,根据这些结果如何使子囊孢子的释放与油菜盛花期相吻合进行了分析讨论. 相似文献
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桦褐孔菌的人工驯化栽培 总被引:10,自引:0,他引:10
对由野生桦褐孔菌茵核分离得到的纯茵种,采用不同配方进行母种、原种、栽培袋培养基对比试验及菌核形成条件的研究。结果表明:母种培养基以葡萄糖、黄豆粉综合培养基为最好;原种培养基以玉米粒为最好;茵核人工代料栽培培养料以桦木屑52%、玉米芯26%、麦麸20%、白糖1%、石膏1%、水分65%为最好,生物学效率达30%。 相似文献
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The use of Contans (Coniothyrium minitans) in Belgian commercial lettuce greenhouses has so far not always fulfilled the expectations of growers. With the aim of improving the efficacy of control of lettuce drop (Sclerotinia sclerotiorum), Contans applied alone and Contans combined with Radix (Trichoderma asperellum and Trichoderma gamsii), Kraft pine lignin and Rovral was compared in three successive lettuce crops in a naturally infested greenhouse. At harvest of each crop, the effect on disease incidence, and the effect on viability and fungal infection of sclerotia buried prior to each crop was evaluated. Contans applied alone negatively affected the sclerotial viability, but did not reduce lettuce drop symptoms. The combination of Contans with Kraft pine lignin, on the other hand, reduced sclerotial viability and lettuce drop compared with the untreated control in the last crop. Furthermore, when Contans was combined with Kraft pine lignin, a higher number of infected sclerotia was observed compared with Contans applied alone. When Radix was added to the combination, there was no extra benefit for the control of lettuce drop. The combination of one Rovral application and Contans resulted in a significant control of lettuce drop together with a negative effect on sclerotial survival. Two Rovral applications, by contrast, reduced the ability of C. minitans to parasitize and kill the sclerotia. To conclude, integration of Contans with a reduced Rovral application and with Kraft pine lignin has potential to improve the control of lettuce drop in commercial greenhouses. 相似文献
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大通县甘蓝型油菜菌核病重发原因及防治对策 总被引:1,自引:0,他引:1
从气候条件、种植方式和田间管理等方面分析了大通县油菜菌核病重发的原因,提出了防治对策。 相似文献
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菌核病是呼伦贝尔盟和呼和浩特市郊蔬菜生产上的重要病害。通过1987~1988年的调查和研究,明确该地区蔬菜菌核病的蔬菜寄主分属6科16种。通过对菌核形态,菌核萌发和子囊盘产生、子囊、子囊孢子和子囊盘尺度,病菌培养性状的比较,肯定呼盟和呼市郊区蔬菜菌核病的病原均为Sclerotinia sclerotiorum(Lib.)de Bary。该病在呼市郊区保护地黄瓜上发病株率常年可达0.24~40.0%,在呼盟保护地青椒上发病株率常年可达10.0——15.0% 相似文献
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研究了两个羊肚菌种在6种栽培料上菌丝的生长情况和菌核的培养特征。结果表明,普通羊肚菌在菌丝生长和菌核形成方面都优于黑脉羊肚菌;以北芪渣为辅料的栽培料效果最佳,菌丝生长快,菌核形成多,其次是杨树根土和草木灰栽培料。 相似文献