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Fernando A. Muoz Sergio Estrada-Parra Andres Romero-Rojas Thierry M. Work Erik Gonzalez-Ballesteros Iris Estrada-Garcia 《Veterinary immunology and immunopathology》2009,131(3-4):211-217
To understand the role of the immune system with respect to disease in reptiles, there is the need to develop tools to assess the host's immune response. An important tool is the development of molecular markers to identify immune cells, and these are limited for reptiles. We developed a technique for the cryopreservation of peripheral blood mononuclear cells and showed that a commercially available anti-CD3 epsilon chain antibody detects a subpopulation of CD3 positive peripheral blood lymphocytes in the marine turtle Chelonia mydas. In the thymus and in skin inoculated with phytohemagglutinin, the same antibody showed the classical staining pattern observed in mammals and birds. For Western blot, the anti-CD3 antibodies identified a 17.6 kDa band in membrane proteins of peripheral blood mononuclear cell compatible in weight to previously described CD3 molecules. This is the first demostration of CD3+ cells in reptiles using specific antibodies. 相似文献
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提升机是采矿企业广泛使用的大功率、高耗能的生产设备之一,矿井提升系统普遍采用电力电子器件构成的整流装置对其直流电机进行供电和控制。提升机在采矿过程中需要频繁地启动、停机,对电网的无功冲击和谐波污染使供电系统的安全稳定性受到严重影响。本文以国内某铅锌矿盲主井提升系统为研究对象,提出晶闸管控制电抗器(Thyristor Controlled Reactors,TCR)和固定电容器组(Fixed Capacitors,FC)相结合的无功补偿与谐波治理方法。针对提升机运行过程的特殊性和复杂性,给出无功补偿装置的具体电路设计、参数计算等的方法。提出的无功补偿与谐波治理技术对无功补偿装置的工程研究和开发提供一定的参考和指导,对提高电网的供电质量,保障矿井安全、稳定、高效生产具有十分重要的意义。 相似文献
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In this study, we found that an intramuscular injection of Japanese flounder (Paralichthys olivaceus, 60–80 g in weight and 15–20 mL in length) with 5 μg of a DNA vaccine (pEGFP‐N2‐LCDV‐cn‐MCP 0.6 kb, containing lymphocystis disease virus major capsid protein gene) induced a strong immune response. Subsequent real‐time polymerase chain reaction showed that the expression of immune‐related genes [e.g., major histocompatibility complex (MHC) class I α, MHC II α, T‐cell receptor (TCR), tumour necrosis factor (TNF), tumour necrosis factor receptor (TNFR), Mx, interleukin (IL)‐1β, CXC and IL‐8R] was significantly changed after DNA vaccination. The most remarkable alternation was the expression of MHC I α and MHC II α genes: MHC II α reached the maximum on day 8 in different tissues, and MHC I α on day 2 in the intestine and gills. The expression of TCR increased and reached a plateau in 2 days in the spleen, gills, kidney and liver after vaccination and then decreased after day 8. In contrast, the expression of TCR in the intestine increased and reached a plateau in 8 days. The expression of IL‐8R reached the maximum on day 2 in different tissues and then decreased on day 8. Mx increased in the gills, kidney, spleen and liver on days 2, 8, 2 and 2, but decreased in the intestine, gills, spleen and liver on days 2, 8, 8 and 8 respectively. The TNFR expression increased in the spleen, kidney and gills on days 2, 8 and 8, but decreased in intestine, liver and gills on days 2, 8 and 8 respectively. The expression of TNF, CXC and IL‐1β increased 2 and 8 days after the injection of DNA vaccine. However, the expression of TNF, CXC and IL‐1β altered on days 2 and 8 with different patterns in different tissues respectively. The fish responded to the DNA vaccine by yielding a specific immunoglobulin against lymphocystis disease virus (LCDV) as observed with indirect ELISA. The DNA vaccine induced a unique humoral response, suggesting that the DNA vaccine activated both cellular and humoral defences of the specific immune system of Japanese flounder. 相似文献
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YIN Qing-song TAN Huo YANG Li-jian CHEN Shao-hua ZHA Xian-feng YE Jing-mei LI Yang-qiu 《园艺学报》2011,27(2):260-267
AIM: To analyze the cytotoxicity of TCR Vα23 - Vβ13 gene-modified T-cells specific to diffuse large B-cell lymphoma(DLBCL) associated antigens in vitro. METHODS: The identified full-length TCR Vβ13 and Vα23 genes of monoclonally expanded T-cells in the peripheral blood from a DLBCL patient were amplified and cloned. The bicistronic recombinant plasmid TCR Vβ13 -IRES-TCR Vα23 was constructed and transfected into T-cells isolated from a healthy individual by NucleofectorTM technology. The mRNA expression of antigen-specific TCR Vα23 and Vβ13 genes and the corresponding proteins were determined by real-time PCR and Western blotting, respectively. The specific cytotoxicity of TCR gene-transferred T-cells in vitro was detected. RESULTS: The recombinant plasmid was constructed successfully and verified by restriction enzyme digestion and nucleotide sequencing. The co-expression of antigen-specific TCR Vα23 and Vβ13 genes at mRNA and protein levels in the transfected healthy T-cells were observed in vitro. Three days after transfection, the cytotoxicity of TCR gene-transduced CD3+T-cells against Toledo cells was significantly higher than that against Raji cells or Molt-4 cells. The DLBCL-specific cytotoxic T-lymphocytes(CTL) were inducted. CONCLUSION: The bicistronic eukaryotic expression plasmid TCR Vβ13 -IRES-TCR Vα23 specific to DLBCL-associated antigens is constructed successfully. The TCR gene-transferred T-cells display the ability of DLBCL-specific cytotoxicity. 相似文献
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马立克氏病病毒感染鸡TCR1与TCR2T细胞动态变化观察 总被引：1，自引：0，他引：1
为深入探讨鸡马立克氏病Ｔ细胞受体亚群的变化与疾病发生与发展的相互关系,本实验以马立克氏病强毒株人工感染１日龄ＳＰＦ雏鸡,在不同的感染期,以流式细胞仪分析脾脏、胸腺、外周血淋巴细胞中ＴＣＲ１＋Ｔ和ＴＣＲ２＋Ｔ细胞的动态变化。结果表明,感染鸡脾脏中ＴＣＲ１＋Ｔ细胞异常增高,而ＴＣＲ２＋Ｔ细胞在脾脏和外周血中表现为暂短的升高,其后迅速下降。ＴＣＲ１＋Ｔ细胞与ＴＣＲ２＋Ｔ细胞的这种异常变化是ｖＭＤＶ感染雏鸡后Ｔ细胞表型变化的重要特征之一 相似文献
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中国北方寒冷地区广泛采用保温措施进行渠道防冻胀,在苯板保温防冻胀设计中铺设厚度一般根据半理论半经验确定,并没有考虑衬砌板与保温板接触热阻及交错布置对保温性能与削减冻胀的影响。该文依据固体材料接触热阻(thermal contact resistance,TCR)原理与压力相关的传热本构模型,提出了混凝土复合保温衬砌新型式。通过ABAQUS有限元软件采用热力耦合模拟将其与普通型式的苯板保温渠道进行比较。结果表明:与普通保温衬砌渠道相比,外界负温时,复合保温衬砌的保温及消减冻胀力效果显著。理论上复合保温衬砌冻胀量消减40%以上,法向冻胀力减少66%,切向冻结力减小58%。对寒区衬砌渠道保温防冻胀设计提供了相应的理论参考。 相似文献
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