Effects of three methionine sources in diets on temporal B0AT1 and ASCT2 mRNA expression in different intestinal segments of cobia,Rachycentron canadum     

Yuanfa He  Shuyan Chi  Beiping Tan  Xiaohui Dong  Qihui Yang  Hongyu Liu  Shuang Zhang 《Journal of the World Aquaculture Society》2021,52(1):171-183

A 4‐week feeding trial was conducted using five groups of hybrid grouper (61.15 ± 0.15 g) to explore the potential effects of three methionine (Met) sources. Five isonitrogenous and isolipidic diets were formulated that included a fishmeal (FM) diet; nonsupplemented (NS) diet; or NS diets with the addition of the L‐methionine (L‐Met), DL‐methionine (DL‐Met), or coated Met (Co‐Met) to obtain the same Met level as the FM diet. Fish were randomly distributed into sea cages (30 fish per cage). Weight gain and specific growth rate in the DL‐Met group and FM group were significantly higher than those in all other groups (p < .05). In the proximal and distal intestines, of the 20 gene and time combinations (10 per gene), there were 14 combinations (70%) in which there were no significant differences in gene expression levels between the FM and DL‐Met groups (p > .05). The main reason for the same growth effect between the DL‐Met and FM groups may be attributed to the synchronized absorption at most time points after feeding, which had similar expression patterns of B⁰AT1 and ASCT2 of the proximal and distal intestines between two groups.  相似文献   

饲粮精料水平和蛋氨酸铬添加剂量对舍饲滩羊生长性能、屠宰性能、肉品质和脂肪沉积的影响     

金亚东  贾柔  周玉香  马青 《动物营养学报》2021,33(2)

本试验旨在探究饲粮精料水平和蛋氨酸铬(Cr-Met)添加剂量对舍饲滩羊生长性能、屠宰性能、肉品质和脂肪沉积的影响。采用2×3双因素试验设计,2个因素分别为饲粮精料水平和Cr-Met添加剂量,其中饲粮精料水平分别设为35%(低精料饲粮,饲粮精粗比为35∶65)和55%(高精料饲粮,饲粮精粗比为55∶45),Cr-Met添加剂量分别设为0、0.75和1.50 g/(d·只)。将60只雄性滩羊羔羊[平均体重为(21±1)kg]随机分配到6个试验组,每组10只。试验期为80 d,其中预试期15 d,正试期65 d。结果显示:1)与低精料饲粮组相比,高精料饲粮组滩羊的平均日增重、屠宰率、背膘厚度、肌内脂肪含量和后腿肉比重显著增加(P<0.05),但料重比与肌肉蒸煮损失、剪切力、pH、亮度(L*)值、红度(a*)值和黄度(b*)值则显著降低(P<0.05)。2)低精料饲粮组滩羊背膘厚度、皮下脂肪厚度、肌内脂肪含量和后腿肉比重随Cr-Met添加剂量的增加而线性降低(P<0.05),但肌肉pH则线性升高(P<0.05)。3)高精料饲粮组滩羊肋肉比重、腰肉比重以及肌肉剪切力和pH随Cr-Met添加剂量的增加而线性升高(P<0.05),而肌内脂肪含量和肌肉a*值则线性降低(P<0.05)。综合本试验测定指标,建议在滩羊养殖中选择精粗比为55∶45的高精料饲粮,Cr-Met的适宜添加剂量为1.50 g/(d·只),且不建议在精粗比为35∶65的低精料饲粮中添加Cr-Met。  相似文献   

13～18周龄笼养蛋鸭适宜蛋白质和蛋氨酸水平的研究   总被引：4，自引：1，他引：3  

赵玉华  王安 《畜牧与兽医》2002,34(9):11-14

用 32 4羽满 1 2周龄的金定鸭 ,通过饲养试验、代谢试验、屠宰试验和对试鸭产蛋期 (1 9～ 2 8周龄 )产蛋性能的观察 ,研究了在等能条件下(ME :1 1 51MJ/kg) ,不同蛋白质水平 (CP :1 3 %、 1 5 %、 1 7% )和蛋氨酸水平 (Met/CP :0 0 1 7、 0 0 2 2、 0 0 2 7)组成的 9种饲粮对 1 3～ 1 8周龄笼养蛋鸭的生长性能、养分利用、体成分沉积和产蛋期产蛋性能的影响。结果表明 :该阶段饲粮粗蛋白、蛋氨酸水平对体增重影响不显著 (P >0 0 5) ;体增重与蛋氨酸 (% )呈一定的正相关 (r=0 4574) ;CP为 1 3%、Met/CP为 0 0 2 7组采食量一直较稳定、较高 ,且饲料效率又好。代谢试验 :不同粗蛋白、蛋氨酸水平饲粮对试鸭氮存留率和能量的利用率影响显著(P <0 0 5或P<0 0 1 ) ,且它们随饲粮CP水平的升高而降低 ,随蛋氨酸水平升高而升高 ;磷的利用率也分别以CP为 1 3%的水平和Met/CP为 0 0 2 7的水平极显著地高于其他水平对磷利用率 (P <0 0 1 )。屠宰试验 :当Met/CP为 0 0 2 7时 ,体氮沉积量和体磷沉积量最佳。产蛋性能 :开产日龄和高峰日龄与 1 3～ 1 8周龄CP食入量呈弱的负相关 (r=- 0 1 83 3和r=- 0 0 984) ,与蛋氨酸食入量呈正相关 (r =0 5780和r =0 435 0 )。饲粮粗蛋白、蛋氨酸水平对 1 9～ 2 8周龄  相似文献   

Growth,Meat Yield,and Economic Responses of Broilers Provided Three- and Four-Phase Schedules Formulated to Moderate and High Nutrient Density During a Fifty-Six-Day Production Period     

《The Journal of Applied Poultry Research》2006,15(2):312-325

This study examined growth responses and meat yield of broilers provided a 3- or 4-phase feeding schedule formulated to moderate or high nutrient density. Four dietary treatments were implemented consisting of high or moderate nutrient density diets that were provided for the duration of 3 phases (1 to 17, 18 to 35, and 36 to 56 d) or 4 phases (1 to 17, 18 to 35, 36 to 46, and 47 to 56 d).Provision of a 3-phase schedule did not alter growth performance, meat yield, or diet cost per final BW. Broilers receiving high nutrient diets had improved growth rate and feed conversion from 1 to 35 d. In the 3-phase schedule, birds given the high nutrient density diets had advantages in BW gain and pectoralis minor breast weight. Market changes in meat prices impact differences in gross feeding margins between diets formulated to high or moderate nutrient density more than variation in diet cost.  相似文献   

不同锌源对断奶小鼠生长及机体抗氧化能力的影响   总被引：9，自引：1，他引：9  

虞泽鹏  乐国伟  施用晖  侯丽 《畜牧与兽医》2005,37(4):1-3

选用断奶小鼠72只作为研究对象,随机分为3组(对照组,硫酸锌组和蛋氨酸锌组),每组设4个重复。测定体重、组织锌含量及相关生理生化指标,以研究硫酸锌、蛋氨酸锌2种不同锌供给形式对机体的生长效应及抗氧化能力的影响。结果表明,硫酸锌组和蛋氨酸锌组均能不同程度地提高小鼠体重,蛋氨酸锌组小鼠体重显著高于对照组与硫酸锌组(P<0.05)。饲粮中添加硫酸锌可提高小鼠肝脏和血清中的锌含量(P>0.05);蛋氨酸锌组小鼠肝脏和血清锌含量高于对照组(P<0.05)及硫酸锌组(P>0.05)。2种锌源均显著提高碱性磷酸酶(AKP)、谷胱甘肽过氧化物酶(GSHPx)活性(P<0.05),不同锌源间无明显差异(P>0.05)。添加不同的锌源不同程度地提高总抗氧化能力(TAOC)、总SOD(TSOD)、铜锌超氧化物歧化酶(CuZnSOD)活性(P<0.05),蛋氨酸锌组TAOC、TSOD显著高于硫酸锌组(P<0.05)。添加锌显著降低NO含量(P<0.05),蛋氨酸锌效果显著强于硫酸锌(P<0.05);一氧化氮合酶(NOS)活性表现出与NO相反的趋势(P>0.05)。  相似文献   

瘤胃保护性蛋氨酸稳定性的研究     

燕磊  杨维仁  杨在宾  姜淑贞  张桂国 《中国草食动物》2005,25(3):13-15

采用不同比例的动物油包被蛋氨酸制成瘤胃保护性蛋氨酸I(RPMetⅠ)、瘤胃保护性蛋氨酸Ⅱ(RPMetⅡ)和瘤胃保护性蛋氨酸III(RPMetⅢ)。通过人工唾液消化试验、体内尼龙袋试验和人工真胃液消化试验,检验DL蛋氨酸(DLMet)、商品过瘤胃蛋氨酸(BypassMet)和瘤胃保护性蛋氨酸(RPMetⅠ、RPMetⅡ和RPMetⅢ)的稳定性。试验结果表明,三种动物油包被蛋氨酸的稳定性均好于商品过瘤胃蛋氨酸和DL-蛋氨酸;不同比例的动物油包被对瘤胃保护性蛋氨酸稳定性的影响差异显著(P<0.05)。  相似文献   

A comparison of two sources of methionine supplemented at different levels on heat shock protein 70 expression and oxidative stress product of Peking ducks subjected to heat stress          下载免费PDF全文

L. Guo  R. Li  Y. F. Zhang  T. Y. Qin  Q. S. Li  X. X. Li  Z. L. Qi 《Journal of animal physiology and animal nutrition》2018,102(1):e147-e154

  相似文献   

过瘤胃氨基酸对泌乳早期奶牛生产性能、乳成分和血液生化指标的影响     

姜富贵  李德鹏  成海建  苏文政  张召坤  时光  宋恩亮 《中国乳业》2021,(7):25-31

为研究日粮添加过瘤胃氨基酸对泌乳早期奶牛生产性能、乳成分、血液生化指标和经济效益的影响,选择96 头体况良好、健康的泌乳早期荷斯坦牛,泌乳天数为（65±15）天。采用单因素随机区组试验设计,按照年龄、胎次和产奶量相近的原则,将96 头奶牛随机分为对照组和试验组,每组48 头。对照组为基础日粮,试验组在对照组日粮的基础上添加过瘤胃蛋氨酸（RPMet）和过瘤胃赖氨酸（RPLys）,添加量分别为18 g/头·天和35 g/头·天。结果表明,与对照组相比,添加RPMet和RPLys显著提高产奶量（P=0.034）,对干物质采食量和饲料转化率无显著影响（P>0.050）;添加RPMet和RPLys显著降低乳尿素氮含量（P=0.015）,对乳脂率有增加的趋势（P=0.096）,对乳蛋白率、乳体细胞数、乳糖率和总固形物含量无显著影响（P>0.050）;添加RPMet和RPLys显著提高血清总蛋白（TP）、白蛋白（ALB）和血糖（GLU）含量,显著降低尿素氮（BUN）含量（P=0.049）,对谷丙转氨酶（ALT）、谷草转氨酶（AST）、球蛋白（GLOB）、碱性磷酸酶（ALP）、甘油三酯（TG）和总胆固醇（TC）含量无显著影响（P>0.050）;添加RPMet和RPLys可提高经济效益3.60 元/头·天。综上所述,日粮添加RPMet和RPLys可提高泌乳早期奶牛的产奶量,增加经济效益。  相似文献   

Formulation challenges of organic poultry diets with readily available ingredients and limited synthetic methionine     

《The Journal of Applied Poultry Research》2016,25(3):443-454

Organic agriculture has increased greatly over the past several years as consumer demand for these products has risen. Organic meat production, including poultry, has grown especially quickly, resulting in increasingly stringent guidelines being put in place to ensure safe, uniform, and ideal products for these consumers. Feed formulation and manufacturing for organic poultry in the United States are strictly regulated by the United States Department of Agriculture National Organic Program. Currently, no synthetic amino acids are allowed to be used in organic poultry diets in the United States except for limited quantities of synthetic methionine since it is typically considered the first or second limiting amino acid in corn and soybean meal based poultry diets and it is a critical nutrient for maintaining bird growth and productive performance, especially regarding egg size in laying hens. The aim of the review herein is to discuss the impacts that current organic regulations on synthetic methionine have on diet formulation when using readily available ingredients. High levels of crude protein must be used to meet bird methionine requirements with this restriction in place, which both increases feed costs and can have negative impacts on bird health and on air quality and the surrounding environment due to increased ammonia emissions. Atypical feed formulation strategies have been explored to address this issue, including such methods as feeding alternative ingredients and providing forage materials. Another possibility would be to use an average lifetime maximum inclusion rate for synthetic methionine, which may allow for better flexibility to provide sufficient methionine throughout various phases of growth and production since the requirements of the bird change with age.  相似文献   

含蛋氨酸二肽影响奶牛乳腺上皮细胞乳蛋白合成相关基因表达   总被引：1，自引：0，他引：1  

陈璐  常晨城  史彬林  高民  赵艳丽  闫素梅 《动物营养学报》2016,(12):316-323

本试验旨在研究含蛋氨酸(Met)二肽对奶牛乳腺上皮细胞(BMECs)内乳蛋白合成相关基因表达的影响。试验分3部分,均采用单因子完全随机试验设计,Met的添加浓度及培养时间分别为60μg/m L(0.402 mmol/L)、48 h。第1部分,培养液添加8种含Met二肽[蛋氨酸-蛋氨酸(P-Met-Met)、蛋氨酸-赖氨酸(P-Met-Lys)、蛋氨酸-色氨酸(P-Met-Trp)、蛋氨酸-苯丙氨酸(P-Met-Phe)、蛋氨酸-苏氨酸(P-Met-Thr)、蛋氨酸-异亮氨酸(P-Met-Ile)、蛋氨酸-亮氨酸(P-Met-Leu)、蛋氨酸-缬氨酸(P-Met-Val)],以不添加二肽为对照,测定BMECs乳蛋白合成相关基因(αs1-酪蛋白、β-酪蛋白、κ-酪蛋白、β-乳球蛋白、Ⅱ型小肽转运载体和氨肽酶氮)的表达量;第2部分,培养液添加8种与上述二肽对应的游离氨基酸(F-Met-Met、F-Met-Lys、F-MetTrp、F-M et-Phe、F-M et-Thr、F-M et-Ile、F-M et-Leu、F-M et-Val),以不添加游离氨基酸为对照,测定BM ECs乳蛋白合成相关基因的表达量;第3部分,用二肽等物质的量替代相应游离氨基酸,测定BMECs乳蛋白合成相关基因的表达量以及细胞内外氨肽酶含量。结果表明:P-Met-Met和P-M et-Lys组较对照组和其他二肽组上调了αs1-酪蛋白和β-酪蛋白基因的表达量,且P-M et-M et组优于P-Met-Lys组。F-Met-Met和F-Met-Lys组较对照组和其他游离氨基酸组显著提高了αs1-酪蛋白基因的表达量(P0.05)。除P-Met-Val和P-Met-Leu组外,其他二肽替代游离氨基酸后均不同程度地提高了乳蛋白和Ⅱ型小肽转运载体基因的表达量,其中P-Met-Met表现出较好的促进效果。总之,含Met二肽等量替代对应的游离氨基酸能够促进乳蛋白基因的表达,其中尤以P-Met-Met的效果最好。  相似文献