抽穗后遮荫对龙粳31产量及籽粒灌浆的影响     

马瑞  黄成亮 《黑龙江农业科学》2017,(2)

对水稻龙粳31抽穗后进行遮光处理,研究遮荫处理与未遮荫处理(CK)在产量及其构成、功能叶片的SPAD值和灌浆特性等方面的差异。结果表明:龙粳31抽穗后进行遮荫,主要影响千粒重和结实率两个产量因素,产量降低达到极显著水平;胁迫功能叶片SPAD值升高;遮光处理的强、弱势粒籽粒最大灌浆速率GR_(max)和平均灌浆速率GR_(mean)均低于对照。  相似文献   

冷凉山区滇杂31“浅肥密嫩”丰产栽培配套技术规程     

周义  樊万刚  徐先捍 《耕作与栽培》2014,(2):61-62

为提高冷凉山区水稻生产水稻生产水平,对黔南州冷凉山区滇杂31“浅肥密嫩”丰产栽培配套技术规程进行了介绍.  相似文献   

流产布鲁氏菌BCSP31基因在大肠杆菌中的可溶性高效表达     

曹小安  邱昌庆  周继章  蔺国珍 《吉林农业大学学报》2007,29(6):683-686

设计1对引物,突变除去布鲁氏菌BCSP31(细胞表面蛋白31)基因的N端信号肽氨基酸序列,经NotⅠ和EcoRⅠ酶切后与相同处理的表达载体pGEX-4T-1连接,转化BL21(DE3)感受态细胞。酶切及PCR扩增鉴定,获得阳性克隆,测序证明其阅读框完全正确。用IPTG诱导表达构建成功的阳性克隆,对产物进行SDS-PAGE和Western-blotting检测,发现表达产物出现预期分子量57 kD的融合蛋白,该蛋白能与牛流产布鲁氏菌阳性血清发生反应,进一步证明目的蛋白主要以可溶性形式存在,表达量约占总蛋白的40%,应用GST琼脂糖凝胶FF纯化可得到纯度较高的表达产物。  相似文献   

T213 L31数值预报产品检验     

尚可政  秦春明  王式功  杨德保  佘峰 《安徽农业科学》2011,39(6):3537-3539,3558

利用国家气象中心T213 L31数值预报产品,对东亚范围内2003~2008年对流层各等压面的高度场、温度场、风场、垂直速度场和水汽场预报进行了检验。结果表明：①高度场预报,可信度较高（即气候距平相关系数大于0.6）的时效,对流层中上部6~7 d,对流层中下部5 d;②温度场预报,可信度较高的时效,对流层中上部4 d,对流层中下部5 d;③风场预报,可信度较高的时效,对流层上部5 d,对流层中部4 d,对流层下部3 d;④垂直速度场预报,可信度较高的时效仅为1~2 d;⑤水汽场预报,可信度较高的时效,对流层中部2 d,对流层下部3~4 d。由以上结果可以推论,T213 L31模式,天气形势和天气过程预报可信度较高的时效为5~7 d;近地面天气系统预报可信度较高的时效约为3~5 d;降水预报可信度较高的时效为1~3 d。  相似文献   

Major outer membrane proteins of Brucella spp.: past,present and future   总被引：16，自引：0，他引：16  

Cloeckaert A  Vizcaíno N  Paquet JY  Bowden RA  Elzer PH 《Veterinary microbiology》2002,90(1-4):229-247

The major outer membrane proteins (OMPs) of Brucella spp. were initially identified in the early 1980s and characterised as potential immunogenic and protective antigens. They were classified according to their apparent molecular mass as 36–38 kDa OMPs or group 2 porin proteins and 31–34 and 25–27 kDa OMPs which belong to the group 3 proteins. The genes encoding the group 2 porin proteins were identified in the late 1980s and consist of two genes, omp2a and omp2b, which are closely linked in the Brucella genome, and which share a great degree of identity (>85%). In the 1990s, two genes were identified coding for the group 3 proteins and were named omp25 and omp31. The predicted amino acid sequences of omp25 and omp31 share 34% identity. The recent release of the genome sequence of B. melitensis 16 M has revealed the presence of five additional gene products homologous to Omp25 and Omp31. The use of recombinant protein technology and monoclonal antibodies (MAbs) has shown that the major OMPs appear to be of little relevance as antigens in smooth (S) B. abortus or B. melitensis infections i.e. low or no protective activity in the mouse model of infection and low or no immunogenicity during host infection. However, group 3 proteins, in particular Omp31, appear as immunodominant antigen in the course of rough (R) B. ovis infection in rams and as important protective antigen in the B. ovis mouse model of infection. The major OMP genes display diversity and specific markers have been identified for Brucella species, biovars, and strains, including the recent marine mammal Brucella isolates for which new species names have been proposed. Recently, Omp25 has been shown to be involved in virulence of B. melitensis, B. abortus and B. ovis. Mutants lacking Omp25 are indeed attenuated in animal models of infection, and moreover provide levels of protection similar or better than currently used attenuated vaccine strain B. melitensis Rev.1. Therefore, these mutant strains appear interesting vaccine candidates for the future. The other group 3 proteins identified in the genome merit also further investigation related to the development of new vaccines.  相似文献   

小麦秆锈菌新小种Ug99及其对我国的影响分析   总被引：3，自引：0，他引：3       下载免费PDF全文

曹远银  韩建东  朱桂清  张璐 《植物保护》2007,33(6):86-89

Ug99(TTKS)是1999年在乌干达首次发现的对最重要小麦抗秆锈病基因Sr31有强毒力的秆锈菌新小种。大量证据显示:该小种不仅具有极其特殊的毒力组合而且传播十分迅速,除在中非乌干达、东非肯尼亚、埃塞俄比亚、苏丹流行外,Ug99现已越过红海、传到了阿拉伯国家也门,以及巴基斯坦的沿海地区,越来越逼近我国。Ug99堪称我国的超毒小种,我国最典型的秆锈菌小种只能分别克服或Sr5或Sr9 e或Sr11单基因抗性,而Ug99不仅具有Sr5、Sr9 e、Sr11的联合毒力,而且还具有Sr21、Sr31、Sr38的联合毒力,而我国从未有小种能克服后者的抗性。1B/1L(含Sr31)易位系曾是我国使用的重要秆锈抗源,必对Ug99高度脆弱,我国118份小麦品种在KARI的测定结果表明,高感品种频率98.3%。一旦Ug99入侵我国,其他流行条件也完全具备。因此,充分作好防范Ug99流行的准备十分必要。  相似文献   

Breed Differences in Natriuretic Peptides in Healthy Dogs     

K. Sjstrand  G. Wess  I. Ljungvall  J. Hggstrm  A‐C. Merveille  M. Wiberg  V. Gouni  J. Lundgren Willesen  S. Hans  A‐S. Lequarr  L. Mejer Srensen  J. Wolf  L. Tiret  M. Kierczak  S. Forsberg  K. McEntee  G. Battaille  E. Seppl  K. Lindblad‐Toh  M. Georges  Hannes Lohi  V. Chetboul  M. Fredholm  K. Hglund 《Journal of veterinary internal medicine / American College of Veterinary Internal Medicine》2014,28(2):451-457

Background

Measurement of plasma concentration of natriuretic peptides (NPs) is suggested to be of value in diagnosis of cardiac disease in dogs, but many factors other than cardiac status may influence their concentrations. Dog breed potentially is 1 such factor.

Objective

To investigate breed variation in plasma concentrations of pro‐atrial natriuretic peptide 31‐67 (proANP 31‐67) and N‐terminal B‐type natriuretic peptide (NT‐proBNP) in healthy dogs.

Animals

535 healthy, privately owned dogs of 9 breeds were examined at 5 centers as part of the European Union (EU) LUPA project.

Methods

Absence of cardiovascular disease or other clinically relevant organ‐related or systemic disease was ensured by thorough clinical investigation. Plasma concentrations of proANP 31‐67 and NT‐proBNP were measured by commercially available ELISA assays.

Results

Overall significant breed differences were found in proANP 31‐67 (P < .0001) and NT‐proBNP (P < .0001) concentrations. Pair‐wise comparisons between breeds differed in approximately 50% of comparisons for proANP 31‐67 as well as NT‐proBNP concentrations, both when including all centers and within each center. Interquartile range was large for many breeds, especially for NT‐proBNP. Among included breeds, Labrador Retrievers and Newfoundlands had highest median NT‐proBNP concentrations with concentrations 3 times as high as those of Dachshunds. German Shepherds and Cavalier King Charles Spaniels had the highest median proANP 31‐67 concentrations, twice the median concentration in Doberman Pinschers.

Conclusions and Clinical Importance

Considerable interbreed variation in plasma NP concentrations was found in healthy dogs. Intrabreed variation was large in several breeds, especially for NT‐proBNP. Additional studies are needed to establish breed‐specific reference ranges.  相似文献   

玉米新品种苏玉31的选育与栽培技术     

张志高  孙杰  王俊仁  吕以忠  施红卫  滕云  李夕峰 《现代农业科技》2014,(3)

介绍了玉米新品种苏玉31的选育过程及特征特性,对其种植密度、氮肥施用技术进行研究,并总结其高产栽培技术,以期为苏玉31的高产栽培提供参考。  相似文献   

Effect of microRNA-155 on regulation of angiogenesis in diabetic rats with cerebral ischemic injury     

HAN Jiang-quan  LU Jun-jiang  XIANG Can-hui  LIU Cheng-ling  WANG Zheng-yuan  LIU Ling  CHEN Ling  FAN Ya-dan 《园艺学报》2015,31(2):354-358

AIM: To evaluate the effect of microRNA-155(miRNA-155) on the regulation of angiogenesis in diabetic rats with cerebral ischemic injury. METHODS: Adult male Sprague-Dawley rats were randomly divided into 5 groups:sham group, cerebral ischemia group, diabetic cerebral ischemia group, diabetic cerebral ischemia+miRNA-155 inhibitors group and diabetic cerebral ischemia+scramble group. Diabetes model was made by injection of streptozocin and permanent cerebral ischemic model was developed by suture-occluded method. The scores of neurological deficit and infarct volume were estimated at 24 h after cerebral ischemia. miRNA-155 level was detected by real-time polymerase chain reaction. The expression of platelet endothelial cell adhesion molecule-1(PECAM-1/CD31) and vascular endothelial growth factor(VEGF) was detected by Western blotting. RESULTS: miRNA-155 inhibitor significantly reduced miRNA-155 levels in the ischemic cortex(P<0.05), improved the scores of neurological deficit, reduced infarction size and upregulated the levels of CD31 and VEGF(P<0.05). CONCLUSION: miRNA-155 has a critical role in the regulation of angiogenesis in diabetic rats with cerebral ischemia. Down-regulation of miRNA-155 using miRNA-155 inhibitor attenuates brain infarct injury in diabetic rats.  相似文献   

Can P NMR spectroscopy be used to indicate the origins of soil organic phosphates?     

M.I. Makarov  L. Haumaier  O.E. Marfenina 《Soil biology & biochemistry》2005,37(1):15-25

The relationship between organic P status of 4 soils, 20 microorganisms isolated from these soils (2 bacteria and 3 fungi for each soil) and 13 dominant plant species of typical natural ecosystems of these soils was evaluated. The soils used were represented by two pairs with different ratios of monoester and diester P, and of DNA and other diester P. A Dystric Podzoluvisol and an alpine Umbric Leptosol were characterized by a relatively high proportion of diester P including much DNA P, while a Calcic Chernozem and subalpine Umbric Leptosol had lower proportion of diesters containing relatively less DNA P. The proportions of P compounds in bacteria and plants were very similar on average, based on the monoester to diester P ratio and on the proportions of different diesters in alkaline extract, whereas fungi contained considerably higher proportions of monoesters and polyphosphates, and a higher proportion of phospholipids in the diester fraction. The results showed that the P_org composition of NaOH extracts from different soils was more similar to the composition of extracts from different groups of microorganisms. There was no clear correspondence between soil and microbial diester P proportion and composition. A high proportion of polyphosphate P including pyrophosphate P in soil extracts indicates a significant contribution of fungal P compounds in the soil while the monoester to diester P ratio, and DNA to non-DNA P ratio should be used with caution to interpret the origins of soil P_org. The relative contributions of microorganisms and plants to monoester and diester P in soils is only partially understood.  相似文献