Inhibition of mycelial growth and sporidial formation in the wheat pathogenNeovossia indica by a polyamine biosynthetic inhibitor     

P. P. Singh  S. Singh  Amarjit S. Basra  P. S. Bedi 《Phytoparasitica》1989,17(4):323-326

DL-α-Difluoromethylornithine (DFMO), a specific suicide inhibitor of the polyamine biosynthetic enzyme ornithine decarboxylase (EC 4.1.1.17), strongly inhibited mycelial growth and sporidial formation of the wheat pathogen,Neovossia indica, in vitro, while DL-α-difluoromethylarginine (DFMA), the analogous suicide inhibitor of arginine decarboxylase (EC 4.1.1.19), did not. The inhibited mycelial growth and sporidial formation were not only restored by putrescine (polyamine) addition, but were actually enhanced in the putrescine + DFMO cultures. Besides altering mycelial growth and morphology, DFMO also reduced the cell size drastically. The inhibition of fungal polyamine biosynthesis is discussed in relation to selective control of plant disease.  相似文献   

柑桔多胺代谢及其对生长结果调控的研究　Ⅰ.柑桔离体叶片NH_3-NH_4~+,精氨酸与腐胺代谢关联的分析     

张秋明  肖浪涛  郑玉生  刘昆玉  谢深喜 《湖南农业大学学报(自然科学版)》1997,(5)

以华盛顿脐橙（Ｃｉｔｒｕｓｓｉｎｅｎｓｉｓ）离体春梢１月龄叶片为试材，在控光（０．５μｍｏｌ／ｍ２ｓ及控温（３０±１）℃条件下进行２４ｈ５０ｍｍｏｌ／ＬＮＨ４Ｃｌ饲喂处理并对相关代谢产物进行了测定．结果表明，叶片ＮＨ３－ＮＨ＋４、游离精氨酸及腐胺含量均直线上升，且呈平行变化；对精氨酸生物合成强度及精氨酸脱羧酶活性的１４Ｃ示踪测定证实了二者的加强是导致精氨酸和腐胺含量增加的主要原因；进一步的直线回归分析显示，上述代谢产物之间及其与精氨酸生物合成强度和精氨酸脱羧酶活性之间存在密切的关联．  相似文献   

腐胺对白桦悬浮细胞中激素和黄酮积累影响的初步研究     

周文洋  孙菲菲  梁佳  詹亚光  范桂枝 《中国农学通报》2015,31(13):23-29

为明确腐胺对白桦悬浮细胞中激素和黄酮累积的影响,在白桦悬浮细胞生长的第8 天添加不同浓度的腐胺、脱落酸(ABA)、茉莉酸甲酯(MeJA)、生长素(IAA)和玉米素核苷(ZR),采用紫外分光光度计法分析白桦悬浮细胞中黄酮含量和利用酶联免疫方法测定ABA、JA、IAA和ZR含量。外源腐胺和激素的添加均不同程度地提高了白桦悬浮细胞中黄酮的累积量,其中1 mmol/L 腐胺处理24 h 时增幅最高,增加了97.90%;激素中1 mg/LABA处理后增幅最高,增加了103.40%。腐胺和激素共同处理后黄酮含量的增幅均较其单独处理提高。其中1 mg/L 腐胺与5 mg/L JA 处理后黄酮含量增幅最高,比对照和腐胺处理分别增加了197.43%和103.57%。同时腐胺处理后增加了IAA 和ZR的含量,而降低了ABA和JA 含量。进一步分析其相关性发现,腐胺处理后激素、激素比与黄酮含量的相关性增幅不同,其中JA与黄酮含量的相关性增幅最大,由对照的-0.883 变为0.903。腐胺与ABA、JA、IAA和ZR协同促进了白桦悬浮细胞中黄酮的累积。  相似文献   

Changes in activity of polyamine biosynthetic enzymes and in polyamine contents in developing fruit tissues of ‘Murcott’ mandarin     

R. Nathan  A. Altman  S.P. Monselise 《Scientia Horticulturae》1984,22(4):359-364

Arginine decarboxylase (ADC), ornithine decarboxylase (ODC), putrescine (Put) and spermidine (Spd) contents were analysed in growing ‘Murcott’ mandarin fruits from bloom to full development. Enzymes of polyamine synthesis and, to a lesser extent, polyamine contents roughly followed patterns of growth activity in fruit tissues, with a maximum at the early stages, a minimum at mid-summer and intermediate values at full fruit development. ADC/ODC and Put/Spd ratios were 2–8 and 2–30, respectively. Possible links between polyamine metabolism and citrus fruit set and development are suggested.  相似文献   

腐胺和脯氨酸对哺乳期仔猪空肠绒毛-隐窝轴上皮细胞的多胺代谢及Wnt信号通路的影响     

王小城  熊霞  杨焕胜  高巍  龚敏  印遇龙 《中国农业科学》2015,48(8):1609-1615

【目的】研究腐胺和脯氨酸对哺乳期仔猪空肠绒毛-隐窝轴上皮细胞的多胺（腐胺、亚精胺和精胺）代谢、Wnt信号通路关键基因的mRNA相对表达量的影响。【方法】选取18头0日龄的刚出生的三元杂交（长白×大白×杜洛克）仔猪,随机配对分成3组,每组6个重复,每个重复1头猪,对照组,腐胺组和脯氨酸组,分别灌喂等体积的生理盐水,5 mg·kg^-1体重添加剂量的腐胺和25 mg·kg^-1体重添加剂量的脯氨酸,到14日龄断奶,断奶后3 d屠宰,分离空肠绒毛-隐窝轴的3个不同分化程度（绒毛顶端,绒毛中段和隐窝）的细胞,分别为F1,F2,F3。高效液相色谱法测定F1,F2,F3中的多胺浓度,RT-PCR测定多胺代谢途径中的相关基因以及Wnt信号通路中关键基因的mRNA相对表达量。【结果】1、在绒毛顶端细胞F1中,与对照组相比,脯氨酸组的腐胺、亚精胺、精胺的浓度均显著增加（P＜0.05）,而腐胺组的多胺均无显著差异（P＞0.05）;在绒毛中段细胞F2中,除了腐胺的含量无显著差异外,脯氨酸组和腐胺组的亚精胺、精胺的浓度均显著高于对照组（P＜0.05）,且脯氨酸组的亚精胺和精胺的浓度均显著高于腐胺组（P＜0.05）;在隐窝底端细胞F3中,腐胺组和脯氨酸组的腐胺、亚精胺、精胺浓度与对照组的浓度差异均不显著（P＞0.05）。2、鸟氨酸脱羧酶(ODC)基因在绒毛顶端上皮细胞F1中的mRNA表达量,腐胺组显著高于脯氨酸组和对照组（P＜0.05）;精氨酸酶（arginase）在F2中的mRNA表达量,脯氨酸组显著高于腐胺组和对照组（P＜0.05）;sFRP3在绒毛中段上皮细胞F2中的mRNA表达量,脯氨酸组的显著高于腐胺组（P＞0.05）;sFRP4在隐窝上皮细胞F3中的mRNA表达量,腐胺组显著高于对照组和脯氨酸组（P＜0.05）。【结论】添加了外源腐胺和脯氨酸,促进了哺乳期仔猪的空肠绒毛-隐窝轴上皮细胞的分化细胞的多胺浓度及促进了多胺的代谢,而对未分化的隐窝细胞没显著影响,同时通过Wnt信号通路调控空肠绒毛-隐窝轴细胞的分化成熟。  相似文献   

Effects of Exogenous Putrescine Application on Hormone Content of Citrus aurantium L. Seedling under Salt Stress     

Muslim Abd Ali Abdulhussein 《农业科学与技术》2014,(6):521-528

A pot experiment was conducted in lath house to study the response of six month-old seedlings of sour orange （Citrus aurantium L.） irrigated with salinity water （1, 2 or 4 ds/m） for four month. The seedling was sprayed with putrescine at 0, 150 or 300 mg/L concentrations for three times with month interval, for investigation the effects of putrrescine spraying on hormonal changes in sour orange seedlings under salt stress. One month after the last putrescine application, the leaves of the vegetative shoots were picked for endogenous plant hormone determination. The results indicate that increasing level of irrigation water salinity to 4 ds/m significantly increased ABA, Put and SA; whereas, decreasing IAA and GA in leave. Spraying vegetative canopy with putreseine at 300 mg/L increased 1AA, SA, ABA and Put, while decreased GA. It can be concluded that the adverse effects of high salinity irrigated water on endogenous plant hormone can be ameliorated, to some extent, by exogenous application of Put at 150 mg/L or 300 mg/L concentration.  相似文献   

盐胁迫下ABA对刺槐幼苗体内腐胺,脯氨酸和保护酶系统的影响   总被引：2，自引：0，他引：2  

沈惠娟  李梅枝 《浙江林学院学报》1992,9(3):290-296

盐胁迫下刺槐幼苗的生长明显受抑,相对含水量(RWC)降低,质膜透性变大,超氧物歧化酶(SOD)和过氧化氢酶(CAT)活性变小,同时大量积累游离脯氨酸和腐胺含量。外源ABA能缓和盐胁迫对生长的抑制,减少质膜透性,提高RWC,SOD和CAT活性,脯氨酸量被继续增加,但腐胺量却被减少,表明腐胺量的变化与幼苗的水分状况和遭受胁迫的程度一致,腐胺积累的多少可作为植物遭受胁迫程度的生理指标。  相似文献   

Protective Effects of Polyamines against Oxidative Stress Induced by Water and Cold Stress in Chickpea   总被引：9，自引：1，他引：9  

H. Nayyar  S. Chander 《Journal of Agronomy and Crop Science》2004,190(5):355-365

Chickpea (Cicer arietinum L.) yields are drastically reduced by water and cold stress that occur individually or simultaneously in northern region of India. The comparative effects of both the stresses were investigated at the metabolic level by examining the endogenous status of polyamines (PAS), active oxygen species and antioxidants. Chickpea plants (15‐day old) growing hydroponically under controlled conditions (light/ dark; 24/21 °C, 16/8 h; irradiance 250 μmol m^?2 s^?1) were subjected to water deficit stress (Ψ_s of ?0.2 to ?1.0 MPa) and cold stress (5–25 °C) for 4 days. LD₅₀ in terms of root growth rate (RGR), electrolyte leakage and triphenyl tetrazolium chloride (TTC) reduction activity was observed at ?0.6 MPa and 10 °C for water and cold stress, respectively. In a subsequent experiment, 15‐day‐old plants were exposed to these stress levels under the above‐mentioned growth conditions for 7 days and analysed for various parameters. In cold‐stressed plants (CS), putrescine (PUT) was observed to be relatively higher while water‐stressed plants (WS) had more of spermidine (SPD). Spermine (SPM) levels increased more rapidly in WS and declined on the fourth day of stress while in CS, a gradual increase occurred that decreased on the seventh day. The accumulation of PAs was short‐lived under the combined presence of both the stresses. Hydrogen peroxide elevated abruptly in WS and remained higher than CS while the latter showed a marked increase in malondialdehyde content. Ascorbic acid increased sharply in WS that decreased on the fourth day while CS showed a relatively gradual increase that reached its maximum on the fourth day and declined subsequently. Glutathione was significantly higher in CS plants in comparison with WS and CS + WS plants. The activity levels of superoxide dismutase were higher up to 4 days and declined subsequently while those of WS stayed higher till the last day of stress. Ascorbate peroxidase levels were significantly higher in CS plants while catalase activity was comparatively more in WS. Exogenous application of PAs reduced the level of hydrogen peroxide, malondialdehyde content and raised the level of antioxidants. Put caused 44 and 32 % increase in RGR in CS and WS, respectively, while SPD resulted in 110 and 25 % enhancement in WS and CS, respectively. Under combination of stresses, RGR increased by 21, 53 and 10 % by Put, SPD and SPM, respectively. The effects of PAs could be reversed largely by their biosynthetic inhibitors. α‐difluoromethylornithine (a biosynthetic inhibitor of putrescine) caused more damage to CS while cyclohexylamine (inhibitor of SPD and SPM biosynthesis) was more inhibitory in WS.  相似文献   

外源腐胺对干旱胁迫下苹果幼苗生理特性的影响     

张媛  韩龙慧  徐继忠 《贵州农业科学》2013,41(7)

为探明干旱胁迫下外源腐胺(Put)对苹果幼苗生理特性的影响,为减轻干旱胁迫对园艺作物的伤害提供理论依据,采用双因素试验设计,即设置不同干旱胁迫水平(轻度和重度)和不同浓度外源Put(1×10-4mol/L和1×10-5mol/L),在盆栽控水方式下,对干旱胁迫下外源Put对苹果幼苗叶片光合特性和可溶性蛋白含量进行研究.结果表明:外源Put提高了干旱胁迫下光饱和时的Pn、Gs,降低了Ci;遮荫〔全天PAR在0～1 000 μmol/(m2·s)〕、干旱胁迫时外源Put对Pn日变化的影响效果不显著;外源Put延缓了干旱胁迫造成的可溶性蛋白含量的下降.综合各指标,1×10-4 mol/L时外源Put对提高苹果幼苗抗旱性的效果较佳.  相似文献   

Putrescine Plays a Positive Role in Salt-Tolerance Mechanisms by Reducing Oxidative Damage in Roots of Vegetable Soybean          下载免费PDF全文

ZHANG Gu-wen  XU Sheng-chun  HU Qi-zan  MAO Wei-hua  GONG Ya-ming 《农业科学学报》2014,13(2):349-357

Polyamines play important roles in plant tolerance to environmental stress. With the aim of investigating the possible involvement of putrescine （Put） in salt-tolerance mechanisms in vegetable soybean roots, exogenous Put （10 mmol L＂） and its biosynthetic inhibitor D-arginine （D-Arg） （0.5 mmol L-1） were added to nutrient solution when vegetable soybean （Glycine max L. cv. Huning 95-1） seedlings were exposed to 100 mmol L^-11 sodium chloride （NaCl）. The results showed that Put ameliorated but D-Arg aggravated the detrimental effects of NaCl on plant growth and biomass production. Under NaCl stress, levels of free, soluble conjugated and insoluble bound types of Put in roots of vegetable soybean were reduced, whereas those of free, soluble conjugated, and insoluble bound types of spermidine （Spd） and spermine （Spm） were increased. Exogenous Put eliminated the decrease in Put but promoted the increase of Spd and Spm. However, these changes could be reversed by D-Arg. Under NaCl stress, activities of arginine decarboxylase （ADC）, S-adenosylmethionine decarboxylase （SAMDC）, diamine oxidase （DAO）, and polyamine oxidase （PAO） were induced, with exogenous Put promoting and D-Arg reversing these changes. Furthermore, NaCl stress decreased activities of antioxidant enzymes. Exogenous Put alleviated but D-Arg exaggerated these effects of NaCl stress, resulting in the same changes in membrane damage and reactive oxygen species （ROS） production. These results indicated that Put plays a positive role in vegetable soybean roots by activating antioxidant enzymes and thereby attenuating oxidative damage.  相似文献