Construction of Brucella abortus A19 VirB Promoter Deletion Strain and Study on Its Biological Characteristics     

DENG Xiaoyu  HE Jinke  YANG Qin  YI Jihai  WANG Yueli  XI Jing  WANG Zhen  CHEN Chuangfu 《中国畜牧兽医》2020,47(3):864-872

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EPS364, a Novel Deep-Sea Bacterial Exopolysaccharide,Inhibits Liver Cancer Cell Growth and Adhesion     

Yun Wang  Ge Liu  Rui Liu  Maosheng Wei  Jinxiang Zhang  Chaomin Sun 《Marine drugs》2021,19(3)

The prognosis of liver cancer was inferior among tumors. New medicine treatments are urgently needed. In this study, a novel exopolysaccharide EPS364 was purified from Vibrio alginolyticus 364, which was isolated from a deep-sea cold seep of the South China Sea. Further research showed that EPS364 consisted of mannose, glucosamine, gluconic acid, galactosamine and arabinose with a molar ratio of 5:9:3.4:0.5:0.8. The relative molecular weight of EPS364 was 14.8 kDa. Our results further revealed that EPS364 was a β-linked and phosphorylated polysaccharide. Notably, EPS364 exhibited a significant antitumor activity, with inducing apoptosis, dissipation of the mitochondrial membrane potential (MMP) and generation of reactive oxygen species (ROS) in Huh7.5 liver cancer cells. Proteomic and quantitative real-time PCR analyses indicated that EPS364 inhibited cancer cell growth and adhesion via targeting the FGF19-FGFR4 signaling pathway. These findings suggest that EPS364 is a promising antitumor agent for pharmacotherapy.  相似文献   

甜瓜新品种-中甜1号的选育     

徐永阳  徐志红  刘君璞 《果树学报》2005,22(3):300-301,F002

中甜1号为优质、高产、耐湿、早熟厚皮甜瓜品种,果实发育期25-28d,果实椭圆形,果皮黄色,上有10条银白色纵沟,果肉白色,单瓜重1.0-1.5kg,可溶性固形物含量13.5%-15.5%。适应性广,在保护地、露地条件下均可栽培。  相似文献   

辣椒新品种亚华205的选育     

张海斌  张章  粟建文  刘虎  刘福清 《长江蔬菜》2003,(1):44-45

亚华205辣椒是由母本2105和父本K6242配制的一代杂种。该品种中早熟，丰产，果实长牛角形，果长18－25cm，横径3．5cm,平均单果重45g，果皮黄色，果面光滑，果条顺直，竦味中等，肉厚，空腔小，耐贮运，商品性好，一般667m^2产量3500－4500kg，适于华南、吕南及华北地区露地丰产栽培。  相似文献   

SLeX和CK19表达与甲状腺乳头状癌浸润转移的关系     

尚培中  柳勇  张星光  张进权  南润玲  谷化平 《河北北方学院学报(自然科学版)》2015,(1):68-70

目的探讨SLeX和CK19蛋白在甲状腺乳头状癌中的表达及其与癌组织浸润转移的关系。方法应用免疫组化染色EliVisionTMplus两步法检测127例甲状腺乳头状癌组织和30例癌旁甲状腺组织中SLeX和CK19蛋白的表达状况。结果甲状腺乳头状癌组织SLeX和CK19总体阳性表达率分别为70.1%和96.1%,显著高于癌旁甲状腺组织(P0.05);存在浸润和伴有淋巴结转移者,SLeX和CK19阳性表达率显著高于无浸润和无淋巴结转移者(P0.05);SLeX与CK19表达具有一定正相关性(r=0.30)。结论 SLeX和CK19在甲状腺乳头状癌组织中多数呈强阳性表达,两者与癌组织浸润转移密切相关。  相似文献   

瓠瓜新品种浙蒲8号的选育     

吴晓花  汪宝根  鲁忠富  徐沛  吴新义  邹宜静  李国景 《中国蔬菜》2015,1(6):61

浙蒲8号是以自交系G7-4-3-1-2-1为母本、自交系J63-1-6-3-2-1为父本配制而成的耐热瓠瓜一代杂种。植株生长势较强,早中熟,以侧蔓结瓜为主,坐瓜性较好。商品瓜中棒形,上下粗细较均匀,平均瓜长约30cm,横径约5cm,瓜皮绿色,具光泽,单瓜质量0.4~0.5kg。耐高温性较强,高温期商品瓜率较对照杭州长瓜高6个百分点;品质佳,游离氨基酸总量1304.193μg·g-1,其中谷氨酸含量85.663μg·g-1;田间对枯萎病和白粉病的抗性强于对照杭州长瓜。适宜作露地栽培和夏秋季设施避雨栽培,夏秋季栽培每667m2产量2600kg以上。  相似文献   

Effect of miR-19a on lipid catabolism in hepatocyte LO2     

TIAN Xiao-ling  LIN Feng-ping  LI Wei-min  LIU Xiu-fen 《园艺学报》2015,31(3):481-484

AIM: To observe the effect of microRNA-19a (miR-19a) on the lipid catabolism of hepatocyte LO2, and to explore the potential mechanism. METHODS: miR-19a was over-expressed or silenced by transfection of miR-19a mimics or miR-19a inhibitor into LO2 cells, then the mRNA level of miR-19a was detected by real-time PCR. The potential target of miR-19a was found by the method of bioinformatics through internet website. The effect of miR-19a on the 3' UTR of peroxisome proliferator-activated receptor α (PPARα) was measured by dual luciferase reporter assay, and the protein level of PPARα and its 2 major downstream rate-limiting enzymes involved in lipid catabolism, acyl-coenzyme a dehydrogenase (ACADM) and carnitine palmitoyltransferase 1A (CPT1A), were detected by Western blotting. Meanwhile, the effect of miR-19a on the generation of ketone body was measured by beta-hydroxybutyric acid (β-OHB) detection assay. RESULTS: The mRNA level of miR-19a was dramatically elevated by the transfection of miR-19a mimics, and sharply decreased by the transfection of miR-19a inhibitor (P<0.05). PPARα was found as a potential target of miR-19a, and dual luciferase reporter assay and Western blotting confirmed the regulatory effect of miR-19a on the expression of PPARα, with the protein level changes of ACADM and CPT1A. miR-19a mimics down-regulated, while miR-19a inhibitor up-regulated the concentration of β-OHB in LO2 cells (P<0.05). CONCLUSION: miR-19a regulates the lipid catabolism of hepatocytes by targeting the PPARα and its 2 downstream rate-limiting enzymes.  相似文献   

Evaluation of a real-time RT-PCR panel for detection of SARS-CoV-2 in bat guano     

Eman Anis  Greg Turner  Julie C. Ellis  Andrew Di Salvo  Amanda Barnard  Susan Carroll  Lisa Murphy 《Journal of veterinary diagnostic investigation》2021,33(2):331

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新冠肺炎疫情与猪肉价格波动初探     

马琳 《猪业科学》2021,38(2):46-49

基于2020年3月-2020年10月猪肉价格及新冠肺炎疫情指数,借助VAR模型,定量研究新冠肺炎疫情对于猪肉价格的影响。研究结果表明:新冠肺炎疫情对猪肉价格存在影响,促使猪肉价格上涨,在此基础上文章提出相应措施。  相似文献   

Evaluation of Safety Measures at a Medical Summer Camp During the SARS-CoV-2 Pandemic     

《The Forestry Chronicle》2023,34(1):77-81

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