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1.
AIM: To investigate the effects of astragaloside IV (AS-IV) on chemokine receptor 4 (CXCR4) and stromal cell-derived factor 1α (SDF-1α) in endothelial progenitor cells (EPCs) and its mechanism. METHODS: Rat bone marrow-derived EPCs were cultured in vitro. The proliferation, adhesion, migration, apoptosis and tube formation capacity of EPCs treated with AS-IV and AMD3100, a specific blocker of CXCR4, were observed. The effects of AS-IV on the expression of SDF-1α/CXCR4 at mRNA and protein levels and the protein level of p-CXCR4 in the EPCs were determined. RESULTS: AS-IV significantly enhanced the proliferation, adhesion, migration and tube formation abilities of EPCs, reduced the apoptosis of EPCs, and up-regulated the mRNA and protein expression of SDF-1α and CXCR4 and the p-CXCR4 protein level in the EPCs. On the other hand, AMD3100 blocked the up-regulating effect of AS-IV on the mRNA and protein expression of CXCR4 and the p-CXCR4 protein level in the EPCs, but did not affect the effect of AS-IV on the expression of SDF-1α. CONCLUSION: AS-IV might enhance the biological function of EPCs by regulating the expression of SDF-1α/CXCR in EPCs. 相似文献
2.
[目的]建立了采用蒸发光散射检测器测定香菊片中黄芪甲苷含量的高效液相色谱法.[方法]色谱柱采用十八烷基硅烷键合硅胶为填充剂(250 mm×4.6 mm,5 μm).流动相为乙腈∶水(32∶68),蒸发光散射检测器.[结果]以峰面积积分值的常用对数(X)对进样量的常用对数(Y)线性回归方程为:Y=1.3346X+5.929 3(r =0.997 7),线性范围为0.516 ～ 10.320μg,回收率为91.0％,RSD为1.8％.[结论]该方法操作简便、测定结果准确、重复性好,可用于香菊片中黄芪甲苷的含量测定. 相似文献
3.
用薄层扫描法测定并比较三种不同皂苷提取方法对黄芪甲苷含量的影响。分别采用大孔树脂吸附法、正丁醇萃取法和KOH回流提取法制备供试品。以氯仿-甲醇-水为展开系统,薄层扫描法测定黄芪甲苷含量。结果显示,大孔树脂吸附法、正丁醇萃取法和KOH回流提取法三种方法提取物中黄芪甲苷平均含量分别为0．07768％,0．05578％,0．06953％。结果显示大孔树脂法提取黄芪所得黄芪甲苷含量较高。 相似文献
4.
AIM To investigate the effects of astragaloside on the levels of sex hormone and oxidative stress in rats with polycystic ovary syndrome （PCOS）. METHODS Female SD rats （n =60） were randomly divided into normal control group, model group, Diane-35 （0.339 2 mg/kg） group, low dose astragaloside （12.5 mg/kg） group and high dose astragaloside （50 mg/kg） group, with 12 rats in each group. The PCOS model was induced by letrozole （1 mg/kg）, which was administered by gavage once a day for 3 weeks. After administration, the estrus cycle of the rats was observed by vaginal smear, and the ovarian index was calculated. HE staining was used to observe the histopathological changes of the ovaries. Serum levels of the sex hormones testosterone （T）, estradiol （E2）, luteinizing hormone （LH） and follicle-stimulating hormone （FSH） were measured by ELISA. The levels of superoxide dismutase （SOD）, malondialdehyde （MDA） and glutathione peroxidase （GSH-Px） in serum and ovarian tissue were detected by colorimetry, and the protein levels of steroidogenetic acute regulatory protein （StAR） and apoptosis-related proteins cleaved caspase-3, Bax and Bcl-2 in ovarian tissue were detected by Western blot. RESULT Compared with control group, the oestrous cycle of the rats in model group was disorder, and the ovarian index was increased, ovary was polycystic. The serum levels of T, LH and MDAwere significantly increased （P <0.05）, while the contents of E2, FSH and the activities of GSH-Px and SOD were significantly decreased （P <0.05）. The levels of MDA, StAR, cleaved caspase-3 and Bax proteins in ovarian tissue were significantly up-regulated （P <0.05）. GSH-Px and SOD activities and Bcl-2 protein levels were significantly down-regulated （P <0.05）. CONCLUSION Astragalosideeffectively balances the levels of sex hormone in PCOS rats and relieves the oxidative stress injury, the mechanism may be related to the inhibition of StAR expression. 相似文献
5.
黄芪是常用补气药之一,黄芪多糖和黄芪甲苷是其发挥药效的物质基础。以黄芪多糖和黄芪甲苷为评价指标,比较甘肃、内蒙古、山西三个不同产地的黄芪饮片的质量差异。结果表明:甘肃黄芪、内蒙古黄芪、山西黄芪的多糖含量分别为22.87%、20.02% 、22.29%;甘肃黄芪的黄芪甲苷含量最高,为0.049%;其次是山西黄芪,黄芪甲苷含量为0.026%,内蒙古黄芪中黄芪甲苷含量最低,仅为0.002 7%。仅有甘肃黄芪的甲苷含量测定结果符合药典标准。分析认为药材生长年限是导致内蒙古黄芪与甘肃黄芪、山西黄芪甲苷含量之间差异较大的主要原因。该研究为黄芪种植、资源开发及合理使用提供参考依据。 相似文献
6.
[目的]优化黄芪（RADIX ASTRAGALI）中黄芪甲苷的提取工艺。[方法]利用均匀设计法,采用小型工业设备TS-NS提取浓缩机组对黄芪进行提取浓缩,考察乙醇浓度、固液比、提取时间及提取次数对黄芪甲苷提取率的影响,并用HPLC-ELSD测定黄芪甲苷的含量,筛选最佳工艺。[结果]黄芪中黄芪甲苷的最佳提取工艺：乙醇浓度为80%,固液比为1∶8,提取次数为3次,提取时间为1 h。[结论]与现有文献报道的方法相比,优选的黄芪甲苷提取工艺简便、可靠,适合工业化生产。 相似文献
7.
[目的]研究低聚壳聚糖植物生长调节剂对黄芪生长及次生代谢物的影响。[方法]利用不同浓度低聚壳聚糖植物生长调节剂对黄芪进行叶面施肥,观察其对黄芪生长情况及次生代谢产物黄芪甲苷、黄芪多糖含量的影响。[结果]适宜浓度的低聚壳聚糖植物生长调节剂能促进黄芪植株及根系的生长,且苗和根粗壮,须根极少。根施叶喷组及根施拌种组的黄芪所含黄芪多糖及黄芪甲苷的含量明显高于空白对照组,说明低聚壳聚糖植物生长调节剂较显著地促进了黄芪多糖、黄芪甲苷含量的提高。[结论]对揭示低聚壳聚糖植物生长调节剂对活性成分的累积效应形成和积累机制,以完善中药材质量调控与药用植物栽培的理论和技术体系及建立优质黄芪的规范化栽培技术,对促进中药材规范化的优良种植都具有重要的价值和意义。 相似文献
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AIM:To study whether astragaloside affects the expression of ATP binding cassette transporter A1 (ABCA1) by regulating miR-33a and promotes the outflow of cholesterol in macrophages. METHODS:In the in vivo experiments, HE staining was used to detect the pathological damage of the cross section of aorta in the mice. The expression of ABCA1 at mRNA and protein levels in mouse aorta was determined by real-time PCR and Western blot. In the in vitro experiments, THP-1 macrophage-derived foam cells were established and then treated with astragaloside-containing serum. Real-time PCR was used to detect the expression of miR-33a. The cells were randomly divided into blank serum group, astragaloside serum group and astragaloside serum+miR-33a mimic group. The expression of ABCA1 at mRNA and protein levels was determined by real-time PCR and Western blot. Oil red O staining and high-performance liquid chromatography were used to detect intracellular lipid content. The method of[3H] incorporation was used to detect intracellular cholesterol outflow. RESULTS:In vivo experiments showed that the blood vessels of the mice in astragaloside group were structurally normal, with neat arrangement, localized small calcified particles, mild lesions, small plaques, reduced foam cells and li-pid, and basically complete elastic plates, indicating that the pathological changes were significantly lighter than those in model group. Compared with model group, the expression of miR-33a in the aorta of the mice in astragaloside group was decreased and the relative expression of ABCA1 at mRNA and protein levels was increased (P<0.05). In vitro experiments showed that astragaloside significantly up-regulated the expression of ABCA1 at mRNA and protein levels, but this effect was inhibited by the transfection of miR-33 mimic without affecting the cell viability. Astragaloside reduced the lipid accumulation in the cells, but this effect was attenuated by miR-33 mimic. Astragaloside reduced intracellular cholesterol accumulation in relation to its promotion of intracellular cholesterol efflux, and the transfection of miR-33a mimic in the cells inhibited cholesterol efflux. CONCLUSION:Astragaloside inhibits the production of miR-33a to increase the expression of ABCA1 and promote the outflow of cholesterol in macrophages. This may be one of the molecular mechanisms of astragaloside in preventing atherosclerosis. 相似文献
9.
Li-feng Huang Yong-ming Yao Jin-feng Li Shu-wen Zhang Wen-xiong Li Ning Dong Yan Yu Zhi-yong Sheng 《Fitoterapia》2012
High mobility group box 1 protein (HMGB1), a potent pro-inflammatory cytokine, contributes to the pathogenesis of diverse inflammatory and infectious disorders. Some studies have illustrated the potential effect of HMGB1 on regulatory T cells (Tregs). Astragaloside IV (AST IV) isolated from a Chinese herb, Astragalus mongholicus, is known to have a variety of immunomodulatory activities. However, it is not yet clear whether AST IV possesses potential regulatory effect on the pro-inflammatory ability of HMGB1 with subsequent activation of Tregs. This study was carried out to investigate the antagonistic effects of different doses of AST IV on the immune function of Tregs mediated by HMGB1 in vitro. Tregs isolated from the spleens of mice were co-cultured with HMGB1 and/or AST IV. Cell phenotypes of Tregs were analyzed, and the contents of various cytokines in the cell supernatants as a result of co-culture and the proliferation of CD4+CD25− T cells were determined. Results showed that HMGB1 stimulation resulted in significantly down-regulation of expressions of Tregs cell phenotypes. However, AST IV can rival the suppressing effect of HMGB1 on immune function of Tregs with a dose-dependent in vitro. These results indicate that AST IV has the potential therapeutic action on inflammation augmented by HMGB1. 相似文献
10.
目的探索黄茂甲葺和三七总皂葺中主要有效成分人参皂葺Rgl、人参皂葺Rbl、三七皂葺R1抗CoCh
诱导的PC12细胞氧化损伤的有效配伍剂量、方法采用CoCh诱导PC12细胞氧化损伤模型,以细胞乳酸脱氢酶
(LDH)漏出率为指标,研究4种有效成分对PC12细胞氧化损伤的有效机制浓度〔在此基础上按UH*(8s)均匀设计实
验,确定4种有效成分的有效配伍剂量〔结果4种有效成分都能抑制CoCI,诱导的PC12细胞LDH的漏出,与损伤组
相比差异有统计学意义(P<0.05 )、且随着药物浓度的增加,对LDH漏出率的抑制作用增强、黄茂甲葺和人参皂葺Rbl
在50 }e,mol儿,人参皂葺Rgl和三七皂葺R1在100 }e,mol儿浓度时LDH漏出抑制率约为80%} UH*(8s)均匀设计实验
多元逐步回归分析得:4种有效成分的8个不同浓度配伍都能抑制CoCI,诱导的PC12细胞LDH的释放,与损伤组相
比差异有统计学意义(P<0.05 )〔人参皂葺Rgl 50 }e,mol儿、黄茂甲葺0.781 25 }e,mol儿、人参皂葺Rbl和三七皂葺R1
各1.562 5 }e,mol/L配伍时抗PC12细胞氧化损伤的效应最强〔结论人参皂葺Rgl 50 }e,mol/L、黄茂甲葺0.781
25 }e,mol儿、人参皂葺Rbl和三七皂葺R1各1.562 5 }e,mol儿配伍组方为抗PC 12细胞氧化损伤的有效配伍剂量〔 相似文献